Basic Information:

  VIRBase ID:  

VHID00491016

  Virus:  

Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)

  Host:  

Homo sapiens

  Confidence Score:  

0.6002

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      kshv-miR-K12-8-5p:  MIMAT0015216          RPL27A:  NM_000990     

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Interactor Information:

	Interactor1	Interactor2
Symbol	kshv-miR-K12-8-5p	RPL27A
miRBase Accession/Entrez ID	MIMAT0015216	6157
Organism	Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	L27A

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	RPL27A	chr11:8708852(+) A-I      3'UTR chr11:8708760(+) A-I      3'UTR chr11:8708858(+) A-I      3'UTR chr11:8708873(+) A-I      3'UTR chr11:8708751(+) A-I      3'UTR chr11:8708787(+) A-I      3'UTR chr11:8708791(+) A-I      3'UTR chr11:8708848(+) A-I      3'UTR chr11:8712331(+) A-I      intronic chr11:8719437(+) A-I      intronic chr11:8718387(+) A-I      intronic chr11:8716349(+) A-I      intronic chr11:8716472(+) A-I      intronic chr11:8716473(+) A-I      intronic chr11:8713657(+) A-I      intronic chr11:8718379(+) A-I      intronic chr11:8719420(+) A-I      intronic chr11:8719443(+) A-I      intronic chr11:8719398(+) A-I      intronic chr11:8708815(+) A-I      3'UTR chr11:8708842(+) A-I      3'UTR chr11:8708831(+) A-I      3'UTR chr11:8707872(+) A-I      3'UTR chr11:8707919(+) A-I      3'UTR chr11:8707930(+) A-I      3'UTR chr11:8707939(+) A-I      3'UTR chr11:8708770(+) A-I      3'UTR chr11:8708794(+) A-I      3'UTR chr11:8708817(+) A-I      3'UTR chr11:8707895(+) A-I      3'UTR chr11:8707834(+) A-I      3'UTR chr11:8708782(+) A-I      3'UTR chr11:8708799(+) A-I      3'UTR chr11:8709429(+) A-I      3'UTR chr11:8709240(+) A-I      3'UTR chr11:8709293(+) A-I      3'UTR chr11:8709305(+) A-I      3'UTR chr11:8709428(+) A-I      3'UTR chr11:8709302(+) A-I      3'UTR chr11:8713324(+) A-I      intronic chr11:8708761(+) A-I      3'UTR chr11:8707958(+) A-I      3'UTR chr11:8709357(+) A-I      3'UTR chr11:8709245(+) A-I      3'UTR chr11:8708771(+) A-I      3'UTR chr11:8708803(+) A-I      3'UTR chr11:8708867(+) A-I      3'UTR chr11:8708786(+) A-I      3'UTR chr11:8708757(+) A-I      3'UTR chr11:8707908(+) A-I      3'UTR chr11:8708785(+) A-I      3'UTR chr11:8709315(+) A-I      3'UTR chr11:8708884(+) A-I      3'UTR chr11:8707961(+) A-I      3'UTR chr11:8708889(+) A-I      3'UTR chr11:8708864(+) A-I      3'UTR chr11:8709382(+) A-I      3'UTR chr11:8708825(+) A-I      3'UTR chr11:8708891(+) A-I      3'UTR chr11:8707952(+) A-I      3'UTR chr11:8707853(+) A-I      3'UTR chr11:8708835(+) A-I      3'UTR chr11:8707907(+) A-I      3'UTR chr11:8707843(+) A-I      3'UTR chr11:8708899(+) A-I      3'UTR chr11:8707822(+) A-I      3'UTR chr11:8707917(+) A-I      3'UTR chr11:8709342(+) A-I      3'UTR chr11:8707944(+) A-I      3'UTR chr11:8707840(+) A-I      3'UTR chr11:8708795(+) A-I      3'UTR chr11:8708796(+) A-I      3'UTR chr11:8708789(+) A-I      3'UTR chr11:8707943(+) A-I      3'UTR

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	RPL27A	chr11:8707907(+) A-I intron -    22327324 chr11:8707908(+) A-I intron -    21960545   //22327324 chr11:8707917(+) A-I intron -    22327324 chr11:8708799(+) A-I intron -    22327324 chr11:8708889(+) A-I intron -    22327324 chr11:8708891(+) A-I intron -    22327324 chr11:8708782(+) A-I intron -    21960545 chr11:8708825(+) A-I intron -    21960545 chr11:8708826(+) A-I intron -    21960545 chr11:8707396(+) C-A intron -    21725310 chr11:8707840(+) A-G intron -    21725310   //22484847 chr11:8707822(+) A-G intron -    22484847 chr11:8707907(+) A-G intron -    22484847 chr11:8707908(+) A-G intron -    22484847 chr11:8707917(+) A-G intron -    22484847 chr11:8707944(+) A-G intron -    22484847 chr11:8707952(+) A-G intron -    22484847 chr11:8707958(+) A-G intron -    22484847 chr11:8707961(+) A-G intron -    22484847 chr11:8708757(+) A-G intron -    22484847 chr11:8708771(+) A-G intron -    22484847 chr11:8708782(+) A-G intron -    22484847 chr11:8708785(+) A-G intron -    22484847 chr11:8708795(+) A-G intron -    22484847 chr11:8708796(+) A-G intron -    22484847 chr11:8708799(+) A-G intron -    22484847 chr11:8708825(+) A-G intron -    22484847 chr11:8708864(+) A-G intron -    22484847 chr11:8708884(+) A-G intron -    22484847 chr11:8708889(+) A-G intron -    22484847 chr11:8708891(+) A-G intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	RPL27A	chr11:8704321-8704322(+) m6A 5'UTR       - chr11:8704405-8704406(+) m6A 5'UTR//intron       - chr11:8704675-8704676(+) m6A 5'UTR//intron       - chr11:8704756-8704757(+) m6A 5'UTR//CDS//exon       - chr11:8704766-8704767(+) m6A 5'UTR//CDS//exon       - chr11:8704774-8704775(+) m6A 5'UTR//CDS//exon       22575960 chr11:8705583-8705584(+) Y 3'UTR//5'UTR//CDS//exon       26075521 chr11:8705642-8705643(+) m6A exon//intron       27773535 chr11:8705696-8705697(+) m6A exon//intron       27773535 chr11:8705872-8705873(+) Gm 5'UTR//exon//intron       - chr11:8706358-8706359(+) m6A 3'UTR//CDS//intron       27773535 chr11:8706372-8706373(+) m6A 3'UTR//CDS//intron       27773535 chr11:8706398-8706399(+) m6A 3'UTR//CDS//intron       27773535 chr11:8706403-8706404(+) m6A 3'UTR//CDS//intron       27773535 chr11:8706487-8706488(+) m6A 3'UTR//intron       27773535 chr11:8706542-8706543(+) m6A 3'UTR//intron       27773535 chr11:8707049-8707050(+) Tm intron       - chr11:8708442-8708443(+) m6A 3'UTR       - chr11:8708449-8708450(+) m6A 3'UTR       - chr11:8708469-8708470(+) m6A 3'UTR       - chr11:8708531-8708532(+) m6A 3'UTR       - chr11:8708549-8708550(+) m6A 3'UTR       - chr11:8708563-8708564(+) m6A 3'UTR       24981863 chr11:8708656-8708657(+) m6A 3'UTR       24981863 chr11:8708672-8708673(+) m6A 3'UTR       24981863 chr11:8709597-8709598(+) m6A 3'UTR       24981863 chr11:8709699-8709700(+) m6A 3'UTR       24981863//22608085 chr11:8709757-8709758(+) m6A 3'UTR       24209618//24981863//22608085 chr11:8709765-8709766(+) m6A 3'UTR       24209618//24981863//22608085 chr11:8710326-8710327(+) m6A 3'UTR       24981863//22608085 chr11:8710359-8710360(+) m6A 3'UTR       24284625//25456834//24981863//22608085 chr11:8710374-8710375(+) m6A 3'UTR       24284625//25456834//24981863//22608085 chr11:8710484-8710485(+) m6A 3'UTR       24284625//25456834//24981863//22608085 chr11:8710489-8710490(+) m6A 3'UTR       24284625//25456834//24981863//22608085 chr11:8710517-8710518(+) m6A 3'UTR       24284625//25456834//24981863//22608085 chr11:8710573-8710574(+) m6A 3'UTR       25456834//24981863//22608085 chr11:8710594-8710595(+) m6A 3'UTR       24981863 chr11:8710780-8710781(+) m6A 3'UTR//exon       24981863//22608085 chr11:8710819-8710820(+) m6A 3'UTR//exon       24981863//22608085 chr11:8710931-8710932(+) m6A 3'UTR//intron       24981863//22608085

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	RPL27A	Chromatin K562 cells     - Cytoplasm HEK-293 cells     28476776 Cytoplasm Nasopharyngeal carcinoma cells     20498841 Cytosol Human myelogenous leukemia cell line (K-562)     21613539 Cytosol HCC cell line (HepG2)     - Exosome Blood     - Nucleus Nasopharyngeal carcinoma cells     20498841 Nucleus HCC cell line (HepG2)|HeLa-S3 cells|K562 cells     - Ribosome-free cytosol Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: kshv-miR-K12-8-5p
Interactor2: RPL27A

Evidence Support:

Weak-Evidence	PAR-CLIP
Prediction-Evidence	miRanda//PARalyzer//Targetscan
Support Database	ViRBase//VIRmiRNA//VmiReg

References:

PMID	22100165	Target region	3'UTR
Source	ViRBase//VIRmiRNA//VmiReg	Interactor1 expression	None
Tissue or cell line	BC-1 cells//BC-3 cells	Interactor2 expression	None
Description	Using PAR-CLIP, a technology which allows the direct and transcriptome-wide identification of miRNA targets, we delineate the target sites for all viral and cellular miRNAs expressed in PEL cell lines. The resulting data set revealed that KSHV miRNAs directly target more than 2000 cellular mRNAs, including many involved in pathways relevant to KSHV pathogenesis. Moreover, 58% of these mRNAs are also targeted by EBV miRNAs, via distinct binding sites.Clusters with seed matches to expressed miRNAs were considered candidate target sites. Of the clusters mapping to human 3'UTRs, 69% (BC-1) and 70% (BC-3) had seed matches to expressed miRNAs (Tables S6).Table S6A BC-1 derived 3'UTR clusters with seed matches to expressed miRNAs.