Basic Information:

  VIRBase ID:  

VHID00471336

  Virus:  

Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)

  Host:  

Homo sapiens

  Confidence Score:  

0.6040

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      kshv-miR-K12-12-5p:  MIMAT0003712          AP2M1:  NM_001311198     

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Interactor Information:

	Interactor1	Interactor2
Symbol	kshv-miR-K12-12-5p	AP2M1
miRBase Accession/Entrez ID	MIMAT0003712	1173
Organism	Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	AP50//CLAPM1//MRD60//mu2

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	AP2M1	chr3:183897499(+) A-I      intronic chr3:183897518(+) A-I      intronic chr3:183897670(+) A-I      intronic chr3:183897671(+) A-I      intronic

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	AP2M1	chr3:183892690-183892691(+) m6A 5'UTR//exon       24284625//24209618//25456834//24981863//27371828//22575960//22608085 chr3:183892740-183892741(+) m6A 5'UTR//exon       24284625//24209618//25456834//24981863//27371828//22575960//22608085 chr3:183894767-183894768(+) m6A 5'UTR//exon       24284625//24209618//24981863//22575960 chr3:183896852-183896853(+) Am CDS//exon       - chr3:183896868-183896869(+) m6A CDS//exon       27773535 chr3:183896877-183896878(+) m6A CDS//exon       27773535 chr3:183896956-183896957(+) m6A exon//intron       27773535 chr3:183897991-183897992(+) m6A CDS//exon       27371828 chr3:183898006-183898007(+) m6A CDS//exon       27371828 chr3:183898056-183898057(+) m6A exon//intron       27371828 chr3:183898751-183898752(+) m6A CDS//exon//intron       22575960 chr3:183899003-183899004(+) m6A CDS//exon       22575960 chr3:183899009-183899010(+) Am CDS//exon       - chr3:183899716-183899717(+) m6A CDS//exon       22575960 chr3:183899771-183899772(+) m6A CDS//exon       22575960 chr3:183899807-183899808(+) m6A CDS//exon       22575960 chr3:183899841-183899842(+) m6A CDS//exon//intron       26404942//22575960 chr3:183899960-183899961(+) m6A CDS//exon       26404942//22575960 chr3:183900648-183900649(+) m6A CDS//exon       22575960 chr3:183901319-183901320(+) m6A CDS//exon       24284625 chr3:183901330-183901331(+) m6A CDS//exon       24284625 chr3:183901392-183901393(+) m6A CDS//exon       24284625//26404942//22575960 chr3:183901565-183901566(+) m6A 3'UTR//exon       24284625//22575960

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	kshv-miR-K12-12-5p	Exosome Primary dendritic cells     21505438
	AP2M1	Chromatin K562 cells     - Cytosol HeLa-S3 cells     - Exosome Blood     - Nucleolus K562 cells     - Nucleoplasm K562 cells     - Nucleus HeLa-S3 cells|K562 cells     - Ribosome Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: kshv-miR-K12-12-5p
Interactor2: AP2M1

Evidence Support:

Weak-Evidence	PAR-CLIP
Prediction-Evidence	miRanda//PARalyzer//RNAHybrid//Targetscan
Support Database	ViRBase//VmiReg

References:

PMID	22100165	Target region	3'UTR
Source	ViRBase//VmiReg	Interactor1 expression	None
Tissue or cell line	BC-3 cells	Interactor2 expression	None
Description	Using PAR-CLIP, a technology which allows the direct and transcriptome-wide identification of miRNA targets, we delineate the target sites for all viral and cellular miRNAs expressed in PEL cell lines. The resulting data set revealed that KSHV miRNAs directly target more than 2000 cellular mRNAs, including many involved in pathways relevant to KSHV pathogenesis. Moreover, 58% of these mRNAs are also targeted by EBV miRNAs, via distinct binding sites.Clusters with seed matches to expressed miRNAs were considered candidate target sites. Of the clusters mapping to human 3'UTRs, 69% (BC-1) and 70% (BC-3) had seed matches to expressed miRNAs (Tables S6).Table S6B BC-3 derived 3'UTR clusters with seed matches to expressed miRNAs.