Basic Information:

  VIRBase ID:  

VHID00468519

  Virus:  

Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)

  Host:  

Homo sapiens

  Confidence Score:  

0.5908

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      kshv-miR-K12-3-5p:  MIMAT0002193          PLCB1:  NM_015192     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	kshv-miR-K12-3-5p	PLCB1
miRBase Accession/Entrez ID	MIMAT0002193	23236
Organism	Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	DEE12//EIEE12//PI-PLC//PLC-154//PLC-beta-1//PLC-I//PLC154//PLCB1A//PLCB1B

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	PLCB1	chr20:8693955(+) A-I      intronic chr20:8417523(+) A-I      intronic chr20:8416776(+) A-I      intronic chr20:8693922(+) A-I      intronic chr20:8693916(+) A-I      intronic chr20:8693915(+) A-I      intronic chr20:8565438(+) A-I      intronic chr20:8565431(+) A-I      intronic chr20:8478890(+) A-I      intronic chr20:8478874(+) A-I      intronic chr20:8416766(+) A-I      intronic chr20:8407689(+) A-I      intronic chr20:8392470(+) A-I      intronic chr20:8390249(+) A-I      intronic chr20:8207415(+) A-I      intronic chr20:8478871(+) A-I      intronic chr20:8508127(+) A-I      intronic chr20:8163322(+) A-I      intronic chr20:8163281(+) A-I      intronic chr20:8122016(+) A-I      intronic chr20:8210755(+) A-I      intronic chr20:8801140(+) A-I      intronic chr20:8508126(+) A-I      intronic chr20:8236676(+) A-I      intronic chr20:8236724(+) A-I      intronic chr20:8784607(+) A-I      intronic chr20:8742737(+) A-I      intronic chr20:8742734(+) A-I      intronic chr20:8508119(+) A-I      intronic chr20:8742712(+) A-I      intronic chr20:8567502(+) A-I      intronic chr20:8508108(+) A-I      intronic chr20:8507230(+) A-I      intronic chr20:8388216(+) A-I      intronic chr20:8388094(+) A-I      intronic chr20:8563539(+) A-I      intronic chr20:8785518(+) A-I      intronic chr20:8563536(+) A-I      intronic chr20:8784614(+) A-I      intronic chr20:8372807(+) A-I      intronic chr20:8244334(+) A-I      intronic chr20:8244333(+) A-I      intronic chr20:8236594(+) A-I      intronic chr20:8429718(+) A-I      intronic chr20:8478870(+) A-I      intronic chr20:8244313(+) A-I      intronic chr20:8784626(+) A-I      intronic chr20:8476162(+) A-I      intronic chr20:8462992(+) A-I      intronic chr20:8462896(+) A-I      intronic chr20:8462890(+) A-I      intronic chr20:8462855(+) A-I      intronic chr20:8742707(+) A-I      intronic chr20:8742706(+) A-I      intronic chr20:8236619(+) A-I      intronic chr20:8244292(+) A-I      intronic chr20:8244229(+) A-I      intronic chr20:8388217(+) A-I      intronic chr20:8236639(+) A-I      intronic chr20:8461684(+) A-I      intronic chr20:8461666(+) A-I      intronic chr20:8461665(+) A-I      intronic chr20:8461658(+) A-I      intronic chr20:8461604(+) A-I      intronic chr20:8461600(+) A-I      intronic chr20:8742687(+) A-I      intronic chr20:8742674(+) A-I      intronic chr20:8742672(+) A-I      intronic chr20:8563533(+) A-I      intronic chr20:8236660(+) A-I      intronic chr20:8563516(+) A-I      intronic chr20:8563542(+) A-I      intronic chr20:8563557(+) A-I      intronic chr20:8563564(+) A-I      intronic chr20:8693959(+) A-I      intronic chr20:8563515(+) A-I      intronic chr20:8563499(+) A-I      intronic chr20:8693956(+) A-I      intronic chr20:8163417(+) A-I      intronic chr20:8163264(+) A-I      intronic chr20:8122189(+) A-I      intronic chr20:8122166(+) A-I      intronic chr20:8122159(+) A-I      intronic chr20:8122150(+) A-I      intronic chr20:8122120(+) A-I      intronic chr20:8584636(+) A-I      intronic chr20:8558026(+) A-I      intronic chr20:8804022(+) A-I      intronic chr20:8803904(+) A-I      intronic chr20:8794357(+) A-I      intronic chr20:8791563(+) A-I      intronic chr20:8791554(+) A-I      intronic chr20:8746474(+) A-I      intronic chr20:8746464(+) A-I      intronic chr20:8746452(+) A-I      intronic chr20:8618717(+) A-I      intronic chr20:8618708(+) A-I      intronic chr20:8478769(+) A-I      intronic chr20:8477011(+) A-I      intronic chr20:8476890(+) A-I      intronic chr20:8455991(+) A-I      intronic chr20:8455977(+) A-I      intronic chr20:8451261(+) A-I      intronic chr20:8435814(+) A-I      intronic chr20:8416757(+) A-I      intronic chr20:8411498(+) A-I      intronic chr20:8411484(+) A-I      intronic chr20:8384286(+) A-I      intronic chr20:8384270(+) A-I      intronic chr20:8210683(+) A-I      intronic chr20:8210632(+) A-I      intronic chr20:8210628(+) A-I      intronic chr20:8803909(+) A-I      intronic chr20:8435811(+) A-I      intronic chr20:8794344(+) A-I      intronic chr20:8476884(+) A-I      intronic chr20:8160687(+) A-I      intronic chr20:8478884(+) A-I      intronic chr20:8314701(+) A-I      intronic chr20:8210666(+) A-I      intronic chr20:8667799(+) A-I      intronic chr20:8212747(+) A-I      intronic chr20:8416942(+) A-I      intronic chr20:8498863(+) A-I      intronic chr20:8451292(+) A-I      intronic chr20:8794331(+) A-I      intronic chr20:8477095(+) A-I      intronic chr20:8534447(+) A-I      intronic chr20:8409041(+) A-I      intronic chr20:8609461(+) A-I      intronic chr20:8383479(+) A-I      intronic chr20:8476318(+) A-I      intronic chr20:8388939(+) A-I      intronic chr20:8162531(+) A-I      intronic chr20:8606596(+) A-I      intronic chr20:8416914(+) A-I      intronic chr20:8784580(+) A-I      intronic chr20:8526815(+) A-I      intronic chr20:8784606(+) A-I      intronic chr20:8477007(+) A-I      intronic chr20:8240995(+) A-I      intronic chr20:8260904(+) A-I      intronic chr20:8554870(+) A-I      intronic chr20:8210421(+) A-I      intronic chr20:8209312(+) A-I      intronic chr20:8207608(+) A-I      intronic chr20:8861696(+) A-I      intronic chr20:8478941(+) A-I      intronic chr20:8502270(+) A-I      intronic chr20:8552049(+) A-I      intronic chr20:8495121(+) A-I      intronic chr20:8383345(+) A-I      intronic chr20:8338201(+) A-I      intronic chr20:8534570(+) A-I      intronic chr20:8570375(+) A-I      intronic chr20:8240921(+) A-I      intronic chr20:8494402(+) A-I      intronic chr20:8462831(+) A-I      intronic chr20:8477030(+) A-I      intronic chr20:8785404(+) A-I      intronic chr20:8574093(+) A-I      intronic chr20:8534594(+) A-I      intronic chr20:8557822(+) A-I      intronic chr20:8453258(+) A-I      intronic chr20:8609417(+) A-I      intronic chr20:8407684(+) A-I      intronic chr20:8411560(+) A-I      intronic chr20:8610917(+) A-I      intronic chr20:8417457(+) A-I      intronic chr20:8461630(+) A-I      intronic chr20:8462851(+) A-I      intronic chr20:8411422(+) A-I      intronic chr20:8574618(+) A-I      intronic chr20:8502165(+) A-I      intronic chr20:8785427(+) A-I      intronic chr20:8240213(+) A-I      intronic chr20:8207399(+) A-I      intronic chr20:8278875(+) A-I      intronic chr20:8574076(+) A-I      intronic chr20:8383321(+) A-I      intronic chr20:8476885(+) A-I      intronic chr20:8161745(+) A-I      intronic chr20:8417598(+) A-I      intronic chr20:8278848(+) A-I      intronic chr20:8212764(+) A-I      intronic chr20:8390372(+) A-I      intronic chr20:8462806(+) A-I      intronic chr20:8609434(+) A-I      intronic chr20:8388937(+) A-I      intronic chr20:8498576(+) A-I      intronic chr20:8160692(+) A-I      intronic chr20:8122226(+) A-I      intronic chr20:8477044(+) A-I      intronic chr20:8357481(+) A-I      intronic chr20:8209272(+) A-I      intronic chr20:8667776(+) A-I      intronic chr20:8423756(+) A-I      intronic chr20:8455978(+) A-I      intronic chr20:8764990(+) A-I      intronic chr20:8162494(+) A-I      intronic chr20:8436276(+) A-I      intronic chr20:8451286(+) A-I      intronic chr20:8570320(+) A-I      intronic chr20:8240797(+) A-I      intronic chr20:8494576(+) A-I      intronic chr20:8392590(+) A-I      intronic chr20:8210413(+) A-I      intronic chr20:8278862(+) A-I      intronic chr20:8785517(+) A-I      intronic

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	PLCB1	chr20:8212710(+) A-I intron -    21960545 chr20:8212733(+) A-I intron -    21960545 chr20:8212760(+) A-I intron -    21960545 chr20:8212764(+) A-I intron -    21960545 chr20:8236554(+) A-I intron -    21960545 chr20:8244292(+) A-I intron -    21960545 chr20:8416897(+) A-I intron -    21960545 chr20:8423157(+) A-I intron -    21960545 chr20:8423158(+) A-I intron -    21960545 chr20:8451005(+) A-I intron -    21960545 chr20:8453281(+) A-I intron -    21960545 chr20:8462851(+) A-I intron -    21960545 chr20:8468703(+) A-I intron -    21960545 chr20:8468864(+) A-I intron -    21960545 chr20:8469371(+) A-I intron -    21960545 chr20:8469374(+) A-I intron -    21960545 chr20:8469459(+) A-I intron -    21960545 chr20:8476885(+) A-I intron -    21960545 chr20:8476893(+) A-I intron -    21960545 chr20:8477030(+) A-I intron -    21960545 chr20:8493279(+) A-I intron -    21960545 chr20:8493281(+) A-I intron -    21960545 chr20:8493298(+) A-I intron -    21960545 chr20:8493335(+) A-I intron -    21960545 chr20:8493356(+) A-I intron -    21960545 chr20:8508060(+) A-I intron -    21960545 chr20:8600225(+) A-I intron -    21960545 chr20:8600251(+) A-I intron -    21960545 chr20:8600252(+) A-I intron -    21960545 chr20:8600732(+) A-I intron -    21960545 chr20:8815720(+) A-I intron -    21960545

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	PLCB1	chr20:8632305-8632306(+) m6A 3'UTR//intron       24981863//27773535//22608085 chr20:8632321-8632322(+) m6A 3'UTR//intron       24981863//27773535//22608085 chr20:8632342-8632343(+) m6A 3'UTR//intron       24284625//24981863//27773535//22608085 chr20:8632378-8632379(+) m6A 3'UTR//intron       24284625//24209618//24981863//27773535//22608085 chr20:8632405-8632406(+) m6A 3'UTR//intron       24284625//24209618//24981863//27773535//22608085 chr20:8632413-8632414(+) m6A 3'UTR//intron       24284625//24209618//24981863//27773535//22608085 chr20:8632455-8632456(+) m6A 3'UTR//intron       24209618//24981863//27773535//22608085 chr20:8632520-8632521(+) m6A 3'UTR//intron       24981863//27773535//22608085 chr20:8632547-8632548(+) m6A 3'UTR//intron       27773535//22608085 chr20:8632570-8632571(+) m6A 3'UTR//intron       - chr20:8639325-8639326(+) m6A CDS//exon       24981863 chr20:8741076-8741077(+) m6A CDS//exon       24981863 chr20:8745799-8745800(+) m6A CDS//exon       24981863//27773535 chr20:8745810-8745811(+) m6A CDS//exon       24981863//27773535 chr20:8745837-8745838(+) m6A CDS//exon       24981863//27773535 chr20:8745849-8745850(+) m6A CDS//exon       24981863//27773535 chr20:8745869-8745870(+) m6A CDS//exon       24981863//27773535 chr20:8745882-8745883(+) m6A CDS//exon       24981863//27773535 chr20:8745895-8745896(+) m6A CDS//exon       24981863//27773535 chr20:8745915-8745916(+) m6A CDS//exon       24981863//27773535 chr20:8745992-8745993(+) m6A CDS//exon       24981863 chr20:8755191-8755192(+) m6A CDS//exon       24981863 chr20:8755232-8755233(+) m6A CDS//exon       24981863 chr20:8755274-8755275(+) m6A CDS//exon       24981863 chr20:8755353-8755354(+) m6A CDS//exon       27773535 chr20:8755362-8755363(+) m6A CDS//exon       27773535 chr20:8769135-8769136(+) m6A CDS//exon       24981863//27773535 chr20:8769350-8769351(+) m6A CDS       24981863//27773535//22608085 chr20:8770171-8770172(+) m6A CDS       24981863//27773535//22608085 chr20:8770853-8770854(+) m6A CDS       24981863//27773535//22608085 chr20:8770865-8770866(+) m6A CDS       24981863//27773535//22608085 chr20:8862299-8862300(+) m6A 3'UTR//CDS//intron       24284625//25456834//24981863//22608085 chr20:8862312-8862313(+) m6A 3'UTR//CDS//intron       24284625//25456834//24981863//22608085 chr20:8862371-8862372(+) m6A 3'UTR//CDS//intron       24284625//25456834//24981863//22608085 chr20:8862410-8862411(+) m6A 3'UTR//CDS//intron       24284625//25456834//24981863//22608085 chr20:8862425-8862426(+) m6A 3'UTR//CDS//intron       24284625//25456834//24981863//22608085 chr20:8862433-8862434(+) m6A 3'UTR//CDS//intron       24284625//25456834//24981863//22608085 chr20:8862461-8862462(+) m6A 3'UTR//CDS//intron       24284625//25456834//24981863//22608085 chr20:8862546-8862547(+) m6A 3'UTR//intron       24284625//25456834//24981863//22608085 chr20:8862578-8862579(+) m6A 3'UTR//intron       24284625//25456834//24981863//22608085 chr20:8862685-8862686(+) m6A 3'UTR//intron       24284625//22608085 chr20:8862782-8862783(+) m6A 3'UTR//intron       24981863//22608085 chr20:8862796-8862797(+) m6A 3'UTR//intron       24981863//22608085 chr20:8863167-8863168(+) m6A 3'UTR//intron       25456834//22608085 chr20:8863225-8863226(+) m6A 3'UTR//intron       25456834//24981863//22608085 chr20:8864566-8864567(+) m6A 3'UTR//intron       22608085 chr20:8864578-8864579(+) m6A 3'UTR//intron       22608085 chr20:8864603-8864604(+) m6A 3'UTR//intron       22608085

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	kshv-miR-K12-3-5p	Exosome B cell line (Raji)|Primary dendritic cells|T cell line (J77)     21505438 Exosome KSHV-infected lymphoma cell lines (BCBL-1|TY-1)     27611973 Exosome K562 cells     24460325
	PLCB1	Chromatin K562 cells     - Cytosol HCC cell line (HepG2)     - Exosome Blood     - Insoluble cytoplasm HCC cell line (HepG2)     - Membrane HCC cell line (HepG2)     - Nucleolus K562 cells     - Nucleoplasm K562 cells     - Nucleus HCC cell line (HepG2)|K562 cells     -

Interaction Network (The top 100 interactions):

Interactor1: kshv-miR-K12-3-5p
Interactor2: PLCB1

Evidence Support:

Weak-Evidence	Microarray
Prediction-Evidence	miRanda//Targetscan
Support Database	ViRBase//VmiReg

References:

PMID	22174674	Target region	None
Source	ViRBase//VmiReg	Interactor1 expression	None
Tissue or cell line	EA.hy926 cells	Interactor2 expression	Downregulation
Description	As KSHV putative direct targets we extracted transcripts that were significantly down-regulated significantly in the replicate experiments, and which contained at least one seed-match to one of the KSHV miRNAs. We identified 704 putative direct targets in DG-75 cells (Figure 3B), and 980 putative direct targets in EA.hy926 cells (Figure 3C). A complete list of putative direct targets can be found in Dataset S2 for DG-75 cells and in Dataset S3 for EA.hy926 cells. The overlap between the two datasets contained 153 putative direct targets (Dataset S4).