Basic Information:

  VIRBase ID:  

VHID00467926

  Virus:  

Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)

  Host:  

Homo sapiens

  Confidence Score:  

0.5908

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      kshv-miR-K12-3-5p:  MIMAT0002193          EIF3D:  NR_156418     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	kshv-miR-K12-3-5p	EIF3D
miRBase Accession/Entrez ID	MIMAT0002193	8664
Organism	Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	eIF3-p66//eIF3-zeta//EIF3S7

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	EIF3D	chr22:36909543(-) A-I      intronic chr22:36913989(-) A-I      intronic chr22:36913966(-) A-I      intronic chr22:36914498(-) A-I      intronic chr22:36909548(-) A-I      intronic chr22:36913968(-) A-I      intronic chr22:36913932(-) A-I      intronic chr22:36911513(-) A-I      intronic chr22:36911512(-) A-I      intronic chr22:36911492(-) A-I      intronic chr22:36911443(-) A-I      intronic chr22:36911435(-) A-I      intronic chr22:36911431(-) A-I      intronic chr22:36911393(-) A-I      intronic chr22:36909577(-) A-I      intronic chr22:36909525(-) A-I      intronic chr22:36909482(-) A-I      intronic chr22:36909446(-) A-I      intronic chr22:36909379(-) A-I      intronic chr22:36909354(-) A-I      intronic chr22:36913841(-) A-I      intronic chr22:36914333(-) A-I      intronic chr22:36913910(-) A-I      intronic chr22:36914009(-) A-I      intronic chr22:36913977(-) A-I      intronic chr22:36914020(-) A-I      intronic chr22:36914381(-) A-I      intronic chr22:36911573(-) A-I      intronic chr22:36914294(-) A-I      intronic chr22:36914067(-) A-I      intronic chr22:36913971(-) A-I      intronic chr22:36923631(-) A-I      intronic chr22:36913824(-) A-I      intronic chr22:36909582(-) A-I      intronic chr22:36914436(-) A-I      intronic chr22:36911488(-) A-I      intronic chr22:36914424(-) A-I      intronic chr22:36909870(-) A-I      intronic chr22:36914320(-) A-I      intronic chr22:36913906(-) A-I      intronic chr22:36911475(-) A-I      intronic chr22:36914388(-) A-I      intronic chr22:36913847(-) A-I      intronic chr22:36909862(-) A-I      intronic chr22:36914474(-) A-I      intronic chr22:36911319(-) A-I      intronic chr22:36911413(-) A-I      intronic chr22:36911331(-) A-I      intronic chr22:36913919(-) A-I      intronic chr22:36913972(-) A-I      intronic chr22:36909631(-) A-I      intronic chr22:36914522(-) A-I      intronic chr22:36913825(-) A-I      intronic chr22:36914458(-) A-I      intronic chr22:36922769(-) A-I      intronic chr22:36913965(-) A-I      intronic chr22:36911572(-) A-I      intronic chr22:36913963(-) A-I      intronic chr22:36914370(-) A-I      intronic chr22:36909452(-) A-I      intronic chr22:36914414(-) A-I      intronic chr22:36911372(-) A-I      intronic chr22:36914453(-) A-I      intronic chr22:36913973(-) A-I      intronic chr22:36909532(-) A-I      intronic

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	EIF3D	chr22:36913825(-) A-I intron -    22327324 chr22:36913974(-) A-I intron -    22327324 chr22:36913977(-) A-I intron -    22327324 chr22:36914381(-) A-I intron -    22327324 chr22:36914522(-) A-I intron -    22327324 chr22:36909792(-) A-G intron -    22484847 chr22:36911572(-) A-G intron -    22484847 chr22:36911573(-) A-G intron -    22484847 chr22:36913825(-) A-G intron -    22484847 chr22:36913965(-) A-G intron -    22484847 chr22:36913971(-) A-G intron -    22484847 chr22:36913972(-) A-G intron -    22484847 chr22:36913973(-) A-G intron -    22484847 chr22:36913974(-) A-G intron -    22484847 chr22:36913977(-) A-G intron -    22484847 chr22:36914020(-) A-G intron -    22484847 chr22:36914381(-) A-G intron -    22484847 chr22:36914436(-) A-G intron -    22484847 chr22:36914522(-) A-G intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	EIF3D	chr22:36906994-36906995(-) m6A 3'UTR//exon       - chr22:36907032-36907033(-) m6A 3'UTR//exon       - chr22:36907038-36907039(-) m6A 3'UTR//CDS//exon       - chr22:36907099-36907100(-) m6A 3'UTR//intron       - chr22:36907139-36907140(-) m6A 3'UTR//intron       - chr22:36907650-36907651(-) m6A CDS//exon       27773535//22575960 chr22:36907806-36907807(-) m6A 3'UTR//CDS//exon       22575960 chr22:36908614-36908615(-) m6A 3'UTR//CDS//exon       22575960 chr22:36912939-36912940(-) m6A exon//intron       26404942 chr22:36913457-36913458(-) m6A 3'UTR//CDS       26404942//27773535 chr22:36913477-36913478(-) m6A CDS       27773535 chr22:36914823-36914824(-) m6A CDS       27773535 chr22:36914843-36914844(-) m6A CDS       27773535 chr22:36914947-36914948(-) m6A CDS       27773535 chr22:36916625-36916626(-) m6A CDS       26404942//27773535 chr22:36916724-36916725(-) m6A CDS       27773535 chr22:36916732-36916733(-) m6A CDS       24981863//27773535 chr22:36919294-36919295(-) m6A CDS//exon       24981863//27773535//22575960 chr22:36919952-36919953(-) m6A CDS//exon       24981863//26404942//27773535//22575960 chr22:36919995-36919996(-) m6A CDS//exon       24981863//26404942//27773535//22575960 chr22:36920010-36920011(-) m6A CDS//exon       24981863//26404942//27773535//22575960 chr22:36920022-36920023(-) m6A CDS//exon       24981863//26404942//27773535//22575960 chr22:36921727-36921728(-) Am CDS//exon       - chr22:36921743-36921744(-) m6A CDS//exon       27773535 chr22:36921748-36921749(-) m6A CDS//exon       27773535 chr22:36921804-36921805(-) m6A exon//intron       - chr22:36921853-36921854(-) m6A exon//intron       - chr22:36921865-36921866(-) m6A exon//intron       - chr22:36922136-36922137(-) m6A CDS//exon       24981863//27773535 chr22:36922942-36922943(-) m6A 5'UTR//intron       24981863 chr22:36924805-36924806(-) m6A 5'UTR//intron       24981863 chr22:36925037-36925038(-) m6A 5'UTR//intron       24981863 chr22:36925072-36925073(-) m6A 5'UTR//intron       24981863

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	kshv-miR-K12-3-5p	Exosome B cell line (Raji)|Primary dendritic cells|T cell line (J77)     21505438 Exosome KSHV-infected lymphoma cell lines (BCBL-1|TY-1)     27611973 Exosome K562 cells     24460325
	EIF3D	Chromatin K562 cells     - Exosome Blood     - Nucleolus K562 cells     - Nucleoplasm K562 cells     - Nucleus HeLa-S3 cells|K562 cells     - Ribosome Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: kshv-miR-K12-3-5p
Interactor2: EIF3D

Evidence Support:

Weak-Evidence	Microarray
Prediction-Evidence	miRanda//Targetscan
Support Database	ViRBase//VmiReg

References:

PMID	22174674	Target region	None
Source	ViRBase//VmiReg	Interactor1 expression	None
Tissue or cell line	DG-75 cells	Interactor2 expression	Downregulation
Description	As KSHV putative direct targets we extracted transcripts that were significantly down-regulated significantly in the replicate experiments, and which contained at least one seed-match to one of the KSHV miRNAs. We identified 704 putative direct targets in DG-75 cells (Figure 3B), and 980 putative direct targets in EA.hy926 cells (Figure 3C). A complete list of putative direct targets can be found in Dataset S2 for DG-75 cells and in Dataset S3 for EA.hy926 cells. The overlap between the two datasets contained 153 putative direct targets (Dataset S4).