Basic Information:

  VIRBase ID:  

VHID00467757

  Virus:  

Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)

  Host:  

Homo sapiens

  Confidence Score:  

0.4752

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      kshv-miR-K12-3-5p:  MIMAT0002193          UBE2D1:  NM_003338     

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Interactor Information:

	Interactor1	Interactor2
Symbol	kshv-miR-K12-3-5p	UBE2D1
miRBase Accession/Entrez ID	MIMAT0002193	7321
Organism	Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	E2(17)KB1//SFT//UBC4/5//UBCH5//UBCH5A

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	UBE2D1	chr10:60105144(+) A-I      intronic chr10:60105124(+) A-I      intronic chr10:60124982(+) A-I      intronic chr10:60105131(+) A-I      intronic chr10:60105002(+) A-I      intronic chr10:60105174(+) A-I      intronic chr10:60105104(+) A-I      intronic chr10:60104976(+) A-I      intronic chr10:60098978(+) A-I      intronic chr10:60098995(+) A-I      intronic chr10:60105137(+) A-I      intronic chr10:60120543(+) A-I      intronic chr10:60104649(+) A-I      intronic chr10:60120509(+) A-I      intronic chr10:60126530(+) A-I      intronic chr10:60124992(+) A-I      intronic chr10:60126581(+) A-I      intronic chr10:60120604(+) A-I      intronic chr10:60105114(+) A-I      intronic chr10:60104975(+) A-I      intronic chr10:60120460(+) A-I      intronic chr10:60120689(+) A-I      intronic chr10:60104834(+) A-I      intronic chr10:60120430(+) A-I      intronic

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	UBE2D1	chr10:60094736-60094737(+) m6A 5'UTR       25456834//24981863//27371828//27773536 chr10:60094808-60094809(+) m6A 5'UTR       24284625//25456834//24981863//27371828//27773536 chr10:60094847-60094848(+) m6A 5'UTR       25456834//24981863//27371828//27773536 chr10:60127766-60127767(+) m6A CDS//exon       27773535 chr10:60128488-60128489(+) m6A CDS//exon       24981863//22608085 chr10:60128504-60128505(+) m6A CDS//exon       24981863//22608085 chr10:60128534-60128535(+) m6A 3'UTR//exon       24981863//27773535//22608085 chr10:60128628-60128629(+) m6A 3'UTR//exon       24981863//27773535//22608085 chr10:60128671-60128672(+) m6A 3'UTR//exon       24981863//27773535//22608085 chr10:60128676-60128677(+) m6A 3'UTR//exon       24981863//27773535//22608085 chr10:60128701-60128702(+) m6A 3'UTR//exon       24981863//27773535//22608085 chr10:60128743-60128744(+) m6A 3'UTR//exon       24981863//22608085 chr10:60128772-60128773(+) m6A 3'UTR//exon       24981863//22608085 chr10:60128840-60128841(+) m6A 3'UTR//exon       22608085

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	kshv-miR-K12-3-5p	Exosome B cell line (Raji)|Primary dendritic cells|T cell line (J77)     21505438 Exosome KSHV-infected lymphoma cell lines (BCBL-1|TY-1)     27611973 Exosome K562 cells     24460325
	UBE2D1	Exosome Blood     - Nucleoplasm K562 cells     -

Interaction Network (The top 100 interactions):

Interactor1: kshv-miR-K12-3-5p
Interactor2: UBE2D1

Evidence Support:

Weak-Evidence	Microarray
Support Database	ViRBase

References:

PMID	22174674	Target region	None
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	DG-75 cells	Interactor2 expression	Downregulation
Description	As KSHV putative direct targets we extracted transcripts that were significantly down-regulated significantly in the replicate experiments, and which contained at least one seed-match to one of the KSHV miRNAs. We identified 704 putative direct targets in DG-75 cells (Figure 3B), and 980 putative direct targets in EA.hy926 cells (Figure 3C). A complete list of putative direct targets can be found in Dataset S2 for DG-75 cells and in Dataset S3 for EA.hy926 cells. The overlap between the two datasets contained 153 putative direct targets (Dataset S4).