Basic Information:

  VIRBase ID:  

VHID00466929

  Virus:  

Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)

  Host:  

Homo sapiens

  Confidence Score:  

0.4752

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      kshv-miR-K12-3-5p:  MIMAT0002193          CPS1:  NR_163592     

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Interactor Information:

	Interactor1	Interactor2
Symbol	kshv-miR-K12-3-5p	CPS1
miRBase Accession/Entrez ID	MIMAT0002193	1373
Organism	Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	CPSASE1//PHN

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	CPS1	chr2:211385082(+) A-I      intronic chr2:211429531(+) A-I      intronic chr2:211429622(+) A-I      intronic chr2:211529120(+) A-I      intronic chr2:211510335(+) A-I      intronic chr2:211539142(+) A-I      intronic

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	CPS1	chr2:211421293-211421294(+) m6A 5'UTR//intron       24981863 chr2:211421348-211421349(+) m6A 5'UTR//intron       24284625//24981863//26404942 chr2:211444459-211444460(+) m6A CDS//exon       22575960 chr2:211444480-211444481(+) Tm CDS//exon       - chr2:211447402-211447403(+) m6A CDS       22575960 chr2:211452806-211452807(+) m6A CDS       22575960 chr2:211452830-211452831(+) m6A CDS       22575960 chr2:211452845-211452846(+) m6A CDS       22575960 chr2:211454860-211454861(+) m6A CDS       22575960 chr2:211454909-211454910(+) m6A CDS       22575960 chr2:211454917-211454918(+) m6A CDS       22575960 chr2:211454933-211454934(+) m6A CDS       22575960 chr2:211456631-211456632(+) m6A CDS       22575960 chr2:211456656-211456657(+) m6A CDS       22575960 chr2:211456684-211456685(+) m6A CDS       22575960 chr2:211457622-211457623(+) m6A CDS       22575960 chr2:211457672-211457673(+) m6A CDS       22575960 chr2:211464232-211464233(+) m6A CDS       22575960 chr2:211464269-211464270(+) m6A CDS       22575960 chr2:211464279-211464280(+) m6A CDS       22575960 chr2:211465285-211465286(+) m6A CDS       22575960 chr2:211465302-211465303(+) Tm CDS       - chr2:211465334-211465335(+) m6A CDS       22575960 chr2:211465365-211465366(+) m6A CDS       22575960 chr2:211465387-211465388(+) m6A CDS       22575960 chr2:211466950-211466951(+) m6A CDS       22575960 chr2:211470193-211470194(+) Am intron       - chr2:211473270-211473271(+) Am CDS       - chr2:211476958-211476959(+) Tm CDS       - chr2:211517185-211517186(+) Am intron       - chr2:211518815-211518816(+) Am CDS       - chr2:211521271-211521272(+) m6A CDS       22575960 chr2:211521293-211521294(+) Cm CDS       - chr2:211521329-211521330(+) m6A CDS       22575960 chr2:211525341-211525342(+) m6A CDS//exon       26404942 chr2:211532929-211532930(+) Y CDS       26075521 chr2:211539660-211539661(+) m6A CDS//exon       22575960 chr2:211540476-211540477(+) m6A CDS//exon       22575960 chr2:211542717-211542718(+) m6A 3'UTR//exon       24981863//26404942 chr2:211542768-211542769(+) m6A 3'UTR//exon       24981863 chr2:211542809-211542810(+) m6A 3'UTR//exon       24981863 chr2:211543435-211543436(+) m6A 3'UTR//exon       22575960 chr2:211543482-211543483(+) m6A 3'UTR//exon       22575960 chr2:211543502-211543503(+) m6A 3'UTR//exon       22575960

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	kshv-miR-K12-3-5p	Exosome B cell line (Raji)|Primary dendritic cells|T cell line (J77)     21505438 Exosome KSHV-infected lymphoma cell lines (BCBL-1|TY-1)     27611973 Exosome K562 cells     24460325
	CPS1	Cytoplasm Nasopharyngeal carcinoma cells     20498841 Cytoplasm Nasopharyngeal carcinoma cells     20498841 Cytoplasm Nasopharyngeal carcinoma cells     20498841 Endoplasmic reticulum Human myelogenous leukemia cell line (K-562)     21613539 Exosome Blood     - Membrane HCC cell line (HepG2)     - Nucleoplasm K562 cells     - Nucleus Nasopharyngeal carcinoma cells     20498841 Nucleus Nasopharyngeal carcinoma cells     20498841 Nucleus Nasopharyngeal carcinoma cells     20498841 Nucleus HCC cell line (HepG2)|HeLa-S3 cells     -

Interaction Network (The top 100 interactions):

Interactor1: kshv-miR-K12-3-5p
Interactor2: CPS1

Evidence Support:

Weak-Evidence	Microarray
Support Database	ViRBase

References:

PMID	22174674	Target region	None
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	EA.hy926 cells	Interactor2 expression	Downregulation
Description	As KSHV putative direct targets we extracted transcripts that were significantly down-regulated significantly in the replicate experiments, and which contained at least one seed-match to one of the KSHV miRNAs. We identified 704 putative direct targets in DG-75 cells (Figure 3B), and 980 putative direct targets in EA.hy926 cells (Figure 3C). A complete list of putative direct targets can be found in Dataset S2 for DG-75 cells and in Dataset S3 for EA.hy926 cells. The overlap between the two datasets contained 153 putative direct targets (Dataset S4).