Basic Information:

  VIRBase ID:  

VHID00465815

  Virus:  

Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)

  Host:  

Homo sapiens

  Confidence Score:  

0.6775

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      kshv-miR-K12-4-3p:  MIMAT0002192          EAF1:  NM_033083     

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Interactor Information:

	Interactor1	Interactor2
Symbol	kshv-miR-K12-4-3p	EAF1
miRBase Accession/Entrez ID	MIMAT0002192	85403
Organism	Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	-

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	EAF1	chr3:15478503(+) A-I      intronic chr3:15483105(+) A-I      3'UTR chr3:15483094(+) A-I      3'UTR chr3:15483134(+) A-I      3'UTR

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	EAF1	chr3:15469049-15469050(+) m6A 5'UTR       24981863 chr3:15469131-15469132(+) m6A 5'UTR       24981863 chr3:15469143-15469144(+) m6A 5'UTR       24284625//24981863 chr3:15469295-15469296(+) m6A CDS       - chr3:15469302-15469303(+) m6A CDS       - chr3:15469314-15469315(+) m6A CDS       - chr3:15469324-15469325(+) m6A CDS       - chr3:15473725-15473726(+) m6A CDS//intron       24981863 chr3:15475890-15475891(+) m6A 3'UTR//CDS       24981863//27773535 chr3:15475980-15475981(+) m6A 3'UTR//CDS       24981863//27773535 chr3:15475987-15475988(+) m6A 3'UTR//CDS       24981863//27773535 chr3:15476019-15476020(+) m6A 3'UTR//CDS       24981863//27773535 chr3:15477876-15477877(+) m6A 3'UTR//CDS       27773535 chr3:15477911-15477912(+) m6A 3'UTR//CDS       - chr3:15477965-15477966(+) m6A 3'UTR//CDS       24209618//24981863 chr3:15478039-15478040(+) m6A 3'UTR//CDS       24284625//24209618//24981863//22608085 chr3:15478073-15478074(+) m6A 3'UTR//CDS       24284625//24209618//24981863//22608085 chr3:15480678-15480679(+) m6A 3'UTR       24284625//24209618//25456834//24981863//22608085 chr3:15480698-15480699(+) m6A 3'UTR       24284625//24209618//25456834//24981863//27773535//22608085 chr3:15480710-15480711(+) m6A 3'UTR       24284625//24209618//25456834//24981863//27773535//22608085 chr3:15480792-15480793(+) m6A 3'UTR       24284625//24209618//25456834//24981863//27773535//22608085 chr3:15480854-15480855(+) m6A 3'UTR       24209618//25456834//24981863//27773535//22608085 chr3:15480882-15480883(+) m6A 3'UTR       24209618//24981863//22608085 chr3:15483144-15483145(+) m6A 3'UTR//intron       25456834 chr3:15483167-15483168(+) m6A 3'UTR//intron       25456834//22608085 chr3:15483209-15483210(+) m6A 3'UTR//intron       25456834//22608085 chr3:15483251-15483252(+) m6A 3'UTR//intron       25456834//22608085 chr3:15483294-15483295(+) m6A 3'UTR//intron       25456834//22608085 chr3:15483324-15483325(+) m6A 3'UTR//intron       25456834 chr3:15483334-15483335(+) m6A 3'UTR//intron       25456834 chr3:15483857-15483858(+) m6A 3'UTR//intron       27773535 chr3:15483885-15483886(+) m6A 3'UTR//intron       27773535 chr3:15483890-15483891(+) m6A 3'UTR//intron       27773535

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	EAF1	Exosome Blood     - Nucleolus K562 cells     - Nucleoplasm K562 cells     - Nucleus HCC cell line (HepG2)|K562 cells     - Ribosome Colon cancer cells     24393600

Related Drug Information:

Resource	Symbol	Drug Name	PubChem ID
RNAactDrug	kshv-miR-K12-4-3p	Panobinostat       6918837 Nilotinib       644241 Irinotecan       60838 Topotecan       60700 l-685,458       5479543 Palbociclib       5330286 Dovitinib       135398510 Crizotinib       11626560 Nvp-aew541       11476171 Pha-665752       10461815

Interaction Network (The top 100 interactions):

Interactor1: kshv-miR-K12-4-3p
Interactor2: EAF1

Evidence Support:

Weak-Evidence	Microarray//PAR-CLIP
Prediction-Evidence	cRep//miRanda//PARalyzer//Targetscan
Support Database	RepTar//ViRBase//VmiReg

References:

[1]PMID	22100165	Target region	3'UTR
Source	RepTar//ViRBase//VmiReg	Interactor1 expression	None
Tissue or cell line	BC-1 cells//BJAB cells	Interactor2 expression	Downregulation
Description	Table S10E Genes that contribute to the enrichments of PAR-CLIP targets of miR-K4-3p from BC-3 libraries in downregulated mRNAs in BJAB/miR-K4-3p cells.

[2]PMID	22174674	Target region	None
Source	RepTar//ViRBase//VmiReg	Interactor1 expression	None
Tissue or cell line	DG-75 cells//EA.hy926 cells	Interactor2 expression	Downregulation
Description	As KSHV putative direct targets we extracted transcripts that were significantly down-regulated significantly in the replicate experiments, and which contained at least one seed-match to one of the KSHV miRNAs. We identified 704 putative direct targets in DG-75 cells (Figure 3B), and 980 putative direct targets in EA.hy926 cells (Figure 3C). A complete list of putative direct targets can be found in Dataset S2 for DG-75 cells and in Dataset S3 for EA.hy926 cells. The overlap between the two datasets contained 153 putative direct targets (Dataset S4).