Basic Information:

  VIRBase ID:  

VHID00465070

  Virus:  

Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)

  Host:  

Homo sapiens

  Confidence Score:  

0.6787

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      kshv-miR-K12-4-3p:  MIMAT0002192          MSL2:  NM_001145417     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	kshv-miR-K12-4-3p	MSL2
miRBase Accession/Entrez ID	MIMAT0002192	55167
Organism	Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	MSL-2//MSL2L1//RNF184

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	MSL2	chr3:135910507(-) A-I      intronic chr3:135898229(-) A-I      intronic chr3:135898220(-) A-I      intronic chr3:135898207(-) A-I      intronic chr3:135898199(-) A-I      intronic chr3:135898204(-) A-I      intronic chr3:135907164(-) A-I      intronic chr3:135907163(-) A-I      intronic chr3:135904530(-) A-I      intronic chr3:135904526(-) A-I      intronic chr3:135904525(-) A-I      intronic chr3:135903993(-) A-I      intronic chr3:135903419(-) A-I      intronic chr3:135903406(-) A-I      intronic chr3:135903026(-) A-I      intronic chr3:135882062(-) A-I      intronic chr3:135882032(-) A-I      intronic chr3:135882017(-) A-I      intronic chr3:135882015(-) A-I      intronic chr3:135881982(-) A-I      intronic chr3:135880957(-) A-I      intronic chr3:135880956(-) A-I      intronic chr3:135880949(-) A-I      intronic chr3:135880842(-) A-I      intronic chr3:135878942(-) A-I      intronic chr3:135878931(-) A-I      intronic chr3:135878929(-) A-I      intronic chr3:135878922(-) A-I      intronic chr3:135876300(-) A-I      intronic chr3:135876287(-) A-I      intronic chr3:135898243(-) A-I      intronic chr3:135898267(-) A-I      intronic chr3:135898271(-) A-I      intronic chr3:135898652(-) A-I      intronic chr3:135898691(-) A-I      intronic chr3:135911145(-) A-I      intronic chr3:135898704(-) A-I      intronic chr3:135898710(-) A-I      intronic chr3:135899359(-) A-I      intronic chr3:135899479(-) A-I      intronic chr3:135899480(-) A-I      intronic chr3:135899482(-) A-I      intronic chr3:135902155(-) A-I      intronic chr3:135902237(-) A-I      intronic chr3:135902893(-) A-I      intronic chr3:135902894(-) A-I      intronic chr3:135907193(-) A-I      intronic chr3:135907194(-) A-I      intronic chr3:135910143(-) A-I      intronic chr3:135910198(-) A-I      intronic chr3:135910426(-) A-I      intronic chr3:135911215(-) A-I      intronic chr3:135911277(-) A-I      intronic chr3:135876269(-) A-I      intronic chr3:135876164(-) A-I      intronic chr3:135871960(-) A-I      intronic chr3:135888485(-) A-I      intronic chr3:135888532(-) A-I      intronic chr3:135888774(-) A-I      intronic chr3:135888785(-) A-I      intronic chr3:135888786(-) A-I      intronic chr3:135889112(-) A-I      intronic chr3:135889456(-) A-I      intronic chr3:135889479(-) A-I      intronic chr3:135888967(-) A-I      intronic chr3:135889489(-) A-I      intronic chr3:135890926(-) A-I      intronic chr3:135891870(-) A-I      intronic chr3:135891875(-) A-I      intronic chr3:135891943(-) A-I      intronic chr3:135897440(-) A-I      intronic chr3:135897545(-) A-I      intronic chr3:135898039(-) A-I      intronic chr3:135888402(-) A-I      intronic chr3:135888399(-) A-I      intronic chr3:135888348(-) A-I      intronic chr3:135885173(-) A-I      intronic chr3:135885143(-) A-I      intronic chr3:135883969(-) A-I      intronic chr3:135883803(-) A-I      intronic chr3:135883755(-) A-I      intronic chr3:135884983(-) A-I      intronic chr3:135904026(-) A-I      intronic chr3:135911293(-) A-I      intronic chr3:135898252(-) A-I      intronic chr3:135889067(-) A-I      intronic chr3:135911185(-) A-I      intronic chr3:135882018(-) A-I      intronic chr3:135888471(-) A-I      intronic chr3:135883936(-) A-I      intronic chr3:135881979(-) A-I      intronic chr3:135911031(-) A-I      intronic chr3:135889519(-) A-I      intronic chr3:135898275(-) A-I      intronic chr3:135903051(-) A-I      intronic chr3:135908417(-) A-I      intronic chr3:135889571(-) A-I      intronic chr3:135883860(-) A-I      intronic chr3:135878857(-) A-I      intronic chr3:135903350(-) A-I      intronic chr3:135879074(-) A-I      intronic chr3:135885148(-) A-I      intronic chr3:135891942(-) A-I      intronic chr3:135890845(-) A-I      intronic chr3:135904077(-) A-I      intronic chr3:135902895(-) A-I      intronic chr3:135883788(-) A-I      intronic chr3:135903186(-) A-I      intronic chr3:135883783(-) A-I      intronic chr3:135889095(-) A-I      intronic chr3:135902372(-) A-I      intronic chr3:135881276(-) A-I      intronic chr3:135881989(-) A-I      intronic chr3:135898705(-) A-I      intronic chr3:135897472(-) A-I      intronic chr3:135896327(-) A-I      intronic chr3:135908322(-) A-I      intronic chr3:135880877(-) A-I      intronic chr3:135908828(-) A-I      intronic chr3:135888940(-) A-I      intronic chr3:135911158(-) A-I      intronic chr3:135881168(-) A-I      intronic chr3:135890811(-) A-I      intronic chr3:135911087(-) A-I      intronic chr3:135910553(-) A-I      intronic chr3:135899895(-) A-I      intronic chr3:135885013(-) A-I      intronic chr3:135899980(-) A-I      intronic chr3:135883837(-) A-I      intronic chr3:135910499(-) A-I      intronic chr3:135888495(-) A-I      intronic chr3:135903323(-) A-I      intronic chr3:135897669(-) A-I      intronic chr3:135888770(-) A-I      intronic chr3:135890904(-) A-I      intronic chr3:135883778(-) A-I      intronic chr3:135888356(-) A-I      intronic chr3:135885081(-) A-I      intronic chr3:135888758(-) A-I      intronic chr3:135907084(-) A-I      intronic chr3:135879006(-) A-I      intronic chr3:135898125(-) A-I      intronic chr3:135890816(-) A-I      intronic chr3:135885082(-) A-I      intronic chr3:135889073(-) A-I      intronic chr3:135910411(-) A-I      intronic chr3:135888333(-) A-I      intronic chr3:135897471(-) A-I      intronic chr3:135910187(-) A-I      intronic chr3:135882049(-) A-I      intronic chr3:135908917(-) A-I      intronic chr3:135906443(-) A-I      intronic chr3:135890825(-) A-I      intronic chr3:135878278(-) A-I      intronic chr3:135904446(-) A-I      intronic chr3:135903200(-) A-I      intronic chr3:135899962(-) A-I      intronic chr3:135883821(-) A-I      intronic chr3:135910117(-) A-I      intronic chr3:135889518(-) A-I      intronic chr3:135899797(-) A-I      intronic chr3:135872024(-) A-I      intronic chr3:135889422(-) A-I      intronic chr3:135891998(-) A-I      intronic chr3:135903954(-) A-I      intronic chr3:135883910(-) A-I      intronic chr3:135903107(-) A-I      intronic chr3:135879031(-) A-I      intronic chr3:135890858(-) A-I      intronic chr3:135904471(-) A-I      intronic chr3:135911100(-) A-I      intronic chr3:135889154(-) A-I      intronic chr3:135898808(-) A-I      intronic chr3:135883848(-) A-I      intronic chr3:135902429(-) A-I      intronic chr3:135876342(-) A-I      intronic chr3:135910253(-) A-I      intronic chr3:135878996(-) A-I      intronic chr3:135910430(-) A-I      intronic chr3:135880945(-) A-I      intronic chr3:135903094(-) A-I      intronic chr3:135888496(-) A-I      intronic chr3:135898314(-) A-I      intronic chr3:135881341(-) A-I      intronic chr3:135888776(-) A-I      intronic chr3:135888736(-) A-I      intronic chr3:135898824(-) A-I      intronic chr3:135885026(-) A-I      intronic chr3:135889083(-) A-I      intronic chr3:135899379(-) A-I      intronic chr3:135888387(-) A-I      intronic chr3:135881256(-) A-I      intronic chr3:135911159(-) A-I      intronic chr3:135903955(-) A-I      intronic chr3:135910365(-) A-I      intronic chr3:135888939(-) A-I      intronic chr3:135889071(-) A-I      intronic chr3:135882001(-) A-I      intronic chr3:135910493(-) A-I      intronic chr3:135877032(-) A-I      intronic

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	MSL2	chr3:135899980(-) A-I intron -    21960545 chr3:135868443(-) C-T exon 3'UTR    21725310 chr3:135877032(-) A-G intron -    22484847 chr3:135880981(-) A-G intron -    22484847 chr3:135880983(-) A-G intron -    22484847 chr3:135882049(-) A-G intron -    22484847 chr3:135885013(-) A-G intron -    22484847 chr3:135885082(-) A-G intron -    22484847 chr3:135888333(-) A-G intron -    22484847 chr3:135888356(-) A-G intron -    22484847 chr3:135888387(-) A-G intron -    22484847 chr3:135888388(-) A-G intron -    22484847 chr3:135888471(-) A-G intron -    22484847 chr3:135888495(-) A-G intron -    22484847 chr3:135888496(-) A-G intron -    22484847 chr3:135888541(-) A-G intron -    22484847 chr3:135888669(-) A-G intron -    22484847 chr3:135888736(-) A-G intron -    22484847 chr3:135888758(-) A-G intron -    22484847 chr3:135888770(-) A-G intron -    22484847 chr3:135888776(-) A-G intron -    22484847 chr3:135888939(-) A-G intron -    22484847 chr3:135888940(-) A-G intron -    22484847 chr3:135888949(-) A-G intron -    22484847 chr3:135889067(-) A-G intron -    22484847 chr3:135889071(-) A-G intron -    22484847 chr3:135889073(-) A-G intron -    22484847 chr3:135889083(-) A-G intron -    22484847 chr3:135889095(-) A-G intron -    22484847 chr3:135889111(-) A-G intron -    22484847 chr3:135889154(-) A-G intron -    22484847 chr3:135889507(-) A-G intron -    22484847 chr3:135889518(-) A-G intron -    22484847 chr3:135889519(-) A-G intron -    22484847 chr3:135889573(-) A-G intron -    22484847 chr3:135890811(-) A-G intron -    22484847 chr3:135890845(-) A-G intron -    22484847 chr3:135891942(-) A-G intron -    22484847 chr3:135896327(-) A-G intron -    22484847 chr3:135898115(-) T-C intron -    22484847 chr3:135898125(-) A-G intron -    22484847 chr3:135898314(-) A-G intron -    22484847 chr3:135898705(-) A-G intron -    22484847 chr3:135898808(-) A-G intron -    22484847 chr3:135899849(-) T-A intron -    22484847 chr3:135899851(-) G-T intron -    22484847 chr3:135899895(-) A-G intron -    22484847 chr3:135899962(-) A-G intron -    22484847 chr3:135899980(-) A-G intron -    22484847 chr3:135902458(-) A-C intron -    22484847 chr3:135903038(-) A-G intron -    22484847 chr3:135903051(-) A-G intron -    22484847 chr3:135903186(-) A-G intron -    22484847 chr3:135903200(-) A-G intron -    22484847 chr3:135903954(-) A-G intron -    22484847 chr3:135903997(-) A-G intron -    22484847 chr3:135904026(-) A-G intron -    22484847 chr3:135904077(-) A-G intron -    22484847 chr3:135908322(-) A-G intron -    22484847 chr3:135910117(-) A-G intron -    22484847 chr3:135910187(-) A-G intron -    22484847 chr3:135910253(-) A-G intron -    22484847 chr3:135910430(-) A-G intron -    22484847 chr3:135910493(-) A-G intron -    22484847 chr3:135910499(-) A-G intron -    22484847 chr3:135910553(-) A-G intron -    22484847 chr3:135910558(-) A-G intron -    22484847 chr3:135911158(-) A-G intron -    22484847 chr3:135911159(-) A-G intron -    22484847 chr3:135911185(-) A-G intron -    22484847 chr3:135911224(-) A-G intron -    22484847 chr3:135911293(-) A-G intron -    22484847 chr3:135911294(-) A-G intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	MSL2	chr3:135868324-135868325(-) m6A 3'UTR       27773535 chr3:135868518-135868519(-) Y 3'UTR       26075521 chr3:135868550-135868551(-) m6A 3'UTR       24209618//24981863 chr3:135868558-135868559(-) m6A 3'UTR       24209618//24981863 chr3:135868571-135868572(-) m6A 3'UTR       24209618//24981863 chr3:135868770-135868771(-) m6A 3'UTR       24981863//26404942 chr3:135868776-135868777(-) m6A 3'UTR       24981863//26404942 chr3:135868832-135868833(-) m6A 3'UTR       24981863 chr3:135869726-135869727(-) m6A 3'UTR       26404942 chr3:135869759-135869760(-) m6A 3'UTR       26404942 chr3:135869775-135869776(-) m6A 3'UTR       26404942 chr3:135869970-135869971(-) m6A 3'UTR       - chr3:135870007-135870008(-) m6A CDS       22608085 chr3:135870155-135870156(-) m6A CDS       24284625//24209618//24981863//27773535//27371828//22575960//22608085 chr3:135870223-135870224(-) m6A CDS       24284625//24209618//24981863//28052920//27773535//27371828//22575960//22608085 chr3:135870357-135870358(-) m6A CDS       24284625//24209618//24981863//28052920//26404942//27773535//27371828//22575960//22608085 chr3:135870371-135870372(-) m6A CDS       24284625//24209618//24981863//28052920//26404942//27773535//27371828//22575960//22608085 chr3:135870430-135870431(-) m6A CDS       24284625//24209618//24981863//28052920//27773535//27371828//22575960//22608085 chr3:135870455-135870456(-) m6A CDS       24284625//24209618//24981863//28052920//27773535//27371828//22575960//22608085 chr3:135870483-135870484(-) m6A CDS       24284625//24209618//24981863//28052920//27773535//27371828//22575960//22608085 chr3:135870524-135870525(-) m6A CDS       24284625//24209618//24981863//28052920//27773535//27371828//22575960//22608085 chr3:135870819-135870820(-) m6A CDS       24284625//25456834//24981863//28052920//27773535//27371828//22575960//22608085 chr3:135870844-135870845(-) m6A CDS       24284625//24209618//25456834//24981863//28052920//27773535//27371828//22575960//22608085 chr3:135870887-135870888(-) m6A CDS       24284625//24209618//25456834//24981863//28052920//27773535//27371828//22575960//22608085 chr3:135870931-135870932(-) m6A CDS       24284625//24209618//25456834//24981863//28052920//27773535//27371828//22575960//22608085 chr3:135870939-135870940(-) m6A CDS       24284625//24209618//25456834//24981863//28052920//27773535//27371828//22575960//22608085 chr3:135871040-135871041(-) m6A CDS       24284625//24209618//24981863//26404942//27773535//22575960//22608085 chr3:135871083-135871084(-) m6A CDS       24284625//24209618//24981863//26404942//27773535//22575960//22608085 chr3:135871227-135871228(-) m6A CDS       24284625//24209618//24981863//27773535//22575960//22608085 chr3:135871287-135871288(-) m6A CDS       24284625//24209618//24981863//27773535//22575960//22608085 chr3:135871295-135871296(-) m6A CDS       24284625//24209618//24981863//27773535//22575960//22608085 chr3:135871385-135871386(-) m6A CDS       24284625//24209618//24981863//26404942//27773535//27371828//22575960//22608085 chr3:135871407-135871408(-) m6A CDS       24284625//24209618//24981863//26404942//27773535//27371828//22575960//22608085 chr3:135871420-135871421(-) m6A CDS       24284625//24209618//24981863//26404942//27773535//27371828//22575960//22608085 chr3:135871444-135871445(-) m6A CDS       24284625//24209618//24981863//28052920//26404942//27773535//27371828//22575960//22608085 chr3:135871465-135871466(-) m6A CDS       24284625//24209618//24981863//28052920//26404942//27773535//27371828//22575960//22608085 chr3:135871488-135871489(-) m6A CDS       24284625//24209618//24981863//27773535//27371828//22575960//22608085 chr3:135871517-135871518(-) m6A 5'UTR//CDS       24284625//24209618//24981863//27773535//27371828//22575960//22608085 chr3:135913329-135913330(-) m6A 5'UTR//intron       24284625//27773535 chr3:135913341-135913342(-) m6A 5'UTR//intron       24284625//27773535 chr3:135913356-135913357(-) m6A 5'UTR//intron       24284625 chr3:135913393-135913394(-) m6A 5'UTR//intron       24284625 chr3:135913884-135913885(-) m6A CDS//intron       24284625//24209618//27773535//22608085 chr3:135913950-135913951(-) m6A CDS//intron       24284625//24209618//24981863//27773535//22608085 chr3:135914105-135914106(-) m6A 5'UTR//intron       24284625//24209618//25456834//24981863//28052920//27773535//27371828//22575960//22608085 chr3:135914205-135914206(-) m6A 5'UTR//intron       24284625//24209618//25456834//24981863//28052920//27773535//27371828//27773536//22575960//22608085 chr3:135914274-135914275(-) m6A 5'UTR//intron       24284625//24209618//25456834//24981863//27371828//27773536//22575960//22608085 chr3:135914624-135914625(-) m6A 5'UTR//intron       24284625//27371828//27773536 chr3:135914652-135914653(-) m6A 5'UTR//intron       24284625//27371828//27773536

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	MSL2	Chromatin HCC cell line (HepG2)|K562 cells     - Exosome Blood     - Nucleoplasm K562 cells     - Nucleus HeLa-S3 cells|K562 cells     -

Related Drug Information:

Resource	Symbol	Drug Name	PubChem ID
RNAactDrug	kshv-miR-K12-4-3p	Panobinostat       6918837 Nilotinib       644241 Irinotecan       60838 Topotecan       60700 l-685,458       5479543 Palbociclib       5330286 Dovitinib       135398510 Crizotinib       11626560 Nvp-aew541       11476171 Pha-665752       10461815

Interaction Network (The top 100 interactions):

Interactor1: kshv-miR-K12-4-3p
Interactor2: MSL2

Evidence Support:

Weak-Evidence	Microarray//PAR-CLIP
Prediction-Evidence	cRep//miRanda//PARalyzer//Rep//Targetscan
Support Database	ViRBase//VIRmiRNA//VmiReg

References:

[1]PMID	22100165	Target region	3'UTR
Source	ViRBase//VIRmiRNA//VmiReg	Interactor1 expression	None
Tissue or cell line	BC-1 cells	Interactor2 expression	None
Description	Using PAR-CLIP, a technology which allows the direct and transcriptome-wide identification of miRNA targets, we delineate the target sites for all viral and cellular miRNAs expressed in PEL cell lines. The resulting data set revealed that KSHV miRNAs directly target more than 2000 cellular mRNAs, including many involved in pathways relevant to KSHV pathogenesis. Moreover, 58% of these mRNAs are also targeted by EBV miRNAs, via distinct binding sites.Clusters with seed matches to expressed miRNAs were considered candidate target sites. Of the clusters mapping to human 3'UTRs, 69% (BC-1) and 70% (BC-3) had seed matches to expressed miRNAs (Tables S6).Table S6A BC-1 derived 3'UTR clusters with seed matches to expressed miRNAs.

[2]PMID	22174674	Target region	None
Source	ViRBase//VIRmiRNA//VmiReg	Interactor1 expression	None
Tissue or cell line	DG-75 cells//EA.hy926 cells	Interactor2 expression	Downregulation
Description	As KSHV putative direct targets we extracted transcripts that were significantly down-regulated significantly in the replicate experiments, and which contained at least one seed-match to one of the KSHV miRNAs. We identified 704 putative direct targets in DG-75 cells (Figure 3B), and 980 putative direct targets in EA.hy926 cells (Figure 3C). A complete list of putative direct targets can be found in Dataset S2 for DG-75 cells and in Dataset S3 for EA.hy926 cells. The overlap between the two datasets contained 153 putative direct targets (Dataset S4).