Basic Information:

  VIRBase ID:  

VHID00464053

  Virus:  

Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)

  Host:  

Homo sapiens

  Confidence Score:  

0.9530

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      kshv-miR-K12-4-3p:  MIMAT0002192          ROCK2:  NM_001321643     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	kshv-miR-K12-4-3p	ROCK2
miRBase Accession/Entrez ID	MIMAT0002192	9475
Organism	Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	ROCK-II

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	ROCK2	chr2:11411360(-) A-I      intronic chr2:11321978(-) A-I      3'UTR chr2:11321960(-) A-I      3'UTR chr2:11321958(-) A-I      3'UTR chr2:11425915(-) A-I      intronic chr2:11425871(-) A-I      intronic chr2:11321941(-) A-I      3'UTR chr2:11328347(-) A-I      intronic chr2:11328342(-) A-I      intronic chr2:11411306(-) A-I      intronic chr2:11411333(-) A-I      intronic chr2:11411331(-) A-I      intronic chr2:11411364(-) A-I      intronic chr2:11411363(-) A-I      intronic chr2:11411326(-) A-I      intronic chr2:11434095(-) A-I      intronic chr2:11409522(-) A-I      intronic chr2:11411324(-) A-I      intronic chr2:11411355(-) A-I      intronic chr2:11411321(-) A-I      intronic chr2:11411354(-) A-I      intronic chr2:11411353(-) A-I      intronic chr2:11411348(-) A-I      intronic chr2:11411347(-) A-I      intronic chr2:11411343(-) A-I      intronic chr2:11411334(-) A-I      intronic chr2:11321975(-) A-I      3'UTR chr2:11411372(-) A-I      intronic chr2:11478253(-) A-I      intronic chr2:11425697(-) A-I      intronic chr2:11425773(-) A-I      intronic chr2:11425805(-) A-I      intronic chr2:11425319(-) A-I      intronic chr2:11425302(-) A-I      intronic chr2:11425226(-) A-I      intronic chr2:11425223(-) A-I      intronic chr2:11422329(-) A-I      intronic chr2:11422314(-) A-I      intronic chr2:11434161(-) A-I      intronic chr2:11435022(-) A-I      intronic chr2:11435078(-) A-I      intronic chr2:11435081(-) A-I      intronic chr2:11435090(-) A-I      intronic chr2:11422310(-) A-I      intronic chr2:11422202(-) A-I      intronic chr2:11435204(-) A-I      intronic chr2:11408045(-) A-I      intronic chr2:11409421(-) A-I      intronic chr2:11409446(-) A-I      intronic chr2:11409455(-) A-I      intronic chr2:11409906(-) A-I      intronic chr2:11409982(-) A-I      intronic chr2:11415226(-) A-I      intronic chr2:11415937(-) A-I      intronic chr2:11418876(-) A-I      intronic chr2:11418845(-) A-I      intronic chr2:11418831(-) A-I      intronic chr2:11418824(-) A-I      intronic chr2:11418807(-) A-I      intronic chr2:11435880(-) A-I      intronic chr2:11389294(-) A-I      intronic chr2:11388079(-) A-I      intronic chr2:11384614(-) A-I      intronic chr2:11378959(-) A-I      intronic chr2:11372352(-) A-I      intronic chr2:11360554(-) A-I      intronic chr2:11346063(-) A-I      intronic chr2:11360475(-) A-I      intronic chr2:11360448(-) A-I      intronic chr2:11360181(-) A-I      intronic chr2:11359513(-) A-I      intronic chr2:11359443(-) A-I      intronic chr2:11359421(-) A-I      intronic chr2:11352206(-) A-I      intronic chr2:11352193(-) A-I      intronic chr2:11352062(-) A-I      intronic chr2:11346086(-) A-I      intronic chr2:11346072(-) A-I      intronic chr2:11478064(-) A-I      intronic chr2:11441865(-) A-I      intronic chr2:11329985(-) A-I      intronic chr2:11328882(-) A-I      intronic chr2:11467968(-) A-I      intronic chr2:11325692(-) A-I      intronic chr2:11325772(-) A-I      intronic chr2:11360250(-) A-I      intronic chr2:11328812(-) A-I      intronic chr2:11328861(-) A-I      intronic chr2:11328855(-) A-I      intronic chr2:11328846(-) A-I      intronic chr2:11476487(-) A-I      intronic chr2:11477826(-) A-I      intronic chr2:11477927(-) A-I      intronic chr2:11328373(-) A-I      intronic chr2:11425876(-) A-I      intronic chr2:11328515(-) A-I      intronic chr2:11328509(-) A-I      intronic chr2:11328429(-) A-I      intronic chr2:11476508(-) A-I      intronic chr2:11330077(-) A-I      intronic chr2:11464433(-) A-I      intronic chr2:11328487(-) A-I      intronic chr2:11328400(-) A-I      intronic chr2:11389186(-) A-I      intronic chr2:11328482(-) A-I      intronic chr2:11359389(-) A-I      intronic chr2:11329925(-) A-I      intronic chr2:11418967(-) A-I      intronic chr2:11407936(-) A-I      intronic chr2:11345991(-) A-I      intronic chr2:11422355(-) A-I      intronic chr2:11328309(-) A-I      intronic chr2:11425796(-) A-I      intronic chr2:11330023(-) A-I      intronic chr2:11425947(-) A-I      intronic chr2:11329141(-) A-I      intronic chr2:11359829(-) A-I      intronic chr2:11415963(-) A-I      intronic chr2:11328374(-) A-I      intronic chr2:11418963(-) A-I      intronic chr2:11330045(-) A-I      intronic chr2:11412952(-) A-I      intronic chr2:11329040(-) A-I      intronic chr2:11328449(-) A-I      intronic chr2:11328297(-) A-I      intronic chr2:11328455(-) A-I      intronic chr2:11360281(-) A-I      intronic chr2:11360343(-) A-I      intronic chr2:11419031(-) A-I      intronic chr2:11410159(-) A-I      intronic chr2:11412755(-) A-I      intronic chr2:11328437(-) A-I      intronic chr2:11419012(-) A-I      intronic chr2:11478413(-) A-I      intronic chr2:11328304(-) A-I      intronic chr2:11442127(-) A-I      intronic chr2:11329079(-) A-I      intronic chr2:11321776(-) A-I      3'UTR chr2:11325795(-) A-I      intronic chr2:11425870(-) A-I      intronic chr2:11423136(-) A-I      intronic chr2:11442140(-) A-I      intronic chr2:11330115(-) A-I      intronic chr2:11360075(-) A-I      intronic chr2:11360272(-) A-I      intronic chr2:11461593(-) A-I      intronic chr2:11360213(-) A-I      intronic chr2:11425810(-) A-I      intronic chr2:11442544(-) A-I      intronic chr2:11387985(-) A-I      intronic chr2:11412874(-) A-I      intronic chr2:11410079(-) A-I      intronic chr2:11388723(-) A-I      intronic chr2:11388752(-) A-I      intronic chr2:11467827(-) A-I      intronic chr2:11442069(-) A-I      intronic chr2:11389227(-) A-I      intronic chr2:11477972(-) A-I      intronic chr2:11415999(-) A-I      intronic chr2:11325650(-) A-I      intronic chr2:11329093(-) A-I      intronic chr2:11418916(-) A-I      intronic chr2:11345987(-) A-I      intronic chr2:11412814(-) A-I      intronic chr2:11373283(-) A-I      intronic chr2:11352218(-) A-I      intronic chr2:11388687(-) A-I      intronic chr2:11329857(-) A-I      intronic chr2:11478466(-) A-I      intronic chr2:11442070(-) A-I      intronic chr2:11442169(-) A-I      intronic chr2:11359515(-) A-I      intronic chr2:11329941(-) A-I      intronic chr2:11435187(-) A-I      intronic chr2:11325651(-) A-I      intronic chr2:11477924(-) A-I      intronic chr2:11418922(-) A-I      intronic chr2:11425853(-) A-I      intronic chr2:11388599(-) A-I      intronic chr2:11329080(-) A-I      intronic chr2:11346194(-) A-I      intronic chr2:11435052(-) A-I      intronic chr2:11352322(-) A-I      intronic chr2:11477870(-) A-I      intronic chr2:11384802(-) A-I      intronic chr2:11425791(-) A-I      intronic chr2:11425770(-) A-I      intronic chr2:11477247(-) A-I      intronic chr2:11442572(-) A-I      intronic chr2:11329086(-) A-I      intronic chr2:11330082(-) A-I      intronic chr2:11372486(-) A-I      intronic chr2:11325874(-) A-I      intronic chr2:11360362(-) A-I      intronic chr2:11476620(-) A-I      intronic chr2:11477892(-) A-I      intronic chr2:11425349(-) A-I      intronic chr2:11342903(-) A-I      intronic chr2:11389197(-) A-I      intronic chr2:11412788(-) A-I      intronic chr2:11410015(-) A-I      intronic chr2:11329149(-) A-I      intronic chr2:11478267(-) A-I      intronic chr2:11360249(-) A-I      intronic chr2:11328279(-) A-I      intronic chr2:11415217(-) A-I      intronic chr2:11328888(-) A-I      intronic chr2:11434028(-) A-I      intronic chr2:11330018(-) A-I      intronic chr2:11329137(-) A-I      intronic chr2:11435068(-) A-I      intronic chr2:11329062(-) A-I      intronic chr2:11388713(-) A-I      intronic chr2:11442562(-) A-I      intronic chr2:11415261(-) A-I      intronic chr2:11467888(-) A-I      intronic chr2:11425694(-) A-I      intronic chr2:11425877(-) A-I      intronic chr2:11389176(-) A-I      intronic chr2:11447708(-) A-I      intronic chr2:11388065(-) A-I      intronic chr2:11409580(-) A-I      intronic chr2:11384800(-) A-I      intronic chr2:11335916(-) A-I      intronic chr2:11477249(-) A-I      intronic

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	ROCK2	chr2:11323239(-) A-I exon 3'UTR    19478186 chr2:11397668(-) A-I intron -    22327324 chr2:11328279(-) A-I intron -    22028664 chr2:11328297(-) A-I intron -    22028664 chr2:11328304(-) A-I intron -    22028664 chr2:11328309(-) A-I intron -    22028664 chr2:11328310(-) A-I intron -    22028664 chr2:11328373(-) A-I intron -    22028664 chr2:11328374(-) A-I intron -    22028664 chr2:11328400(-) A-I intron -    22028664 chr2:11328429(-) A-I intron -    22028664 chr2:11328437(-) A-I intron -    22028664 chr2:11328449(-) A-I intron -    22028664 chr2:11328455(-) A-I intron -    22028664 chr2:11328482(-) A-I intron -    22028664 chr2:11328487(-) A-I intron -    22028664 chr2:11328509(-) A-I intron -    22028664 chr2:11328515(-) A-I intron -    22028664 chr2:11329086(-) A-G intron -    22484847 chr2:11329137(-) A-G intron -    22484847 chr2:11329925(-) A-G intron -    22484847 chr2:11329991(-) A-G intron -    22484847 chr2:11329995(-) A-G intron -    22484847 chr2:11330071(-) A-G intron -    22484847 chr2:11330107(-) A-G intron -    22484847 chr2:11330115(-) A-G intron -    22484847 chr2:11359389(-) A-G intron -    22484847 chr2:11389159(-) A-G intron -    22484847 chr2:11389176(-) A-G intron -    22484847 chr2:11389186(-) A-G intron -    22484847 chr2:11389197(-) A-G intron -    22484847 chr2:11389227(-) A-G intron -    22484847 chr2:11412755(-) A-G intron -    22484847 chr2:11412788(-) A-G intron -    22484847 chr2:11415217(-) A-G intron -    22484847 chr2:11415261(-) A-G intron -    22484847 chr2:11418963(-) A-G intron -    22484847 chr2:11418967(-) A-G intron -    22484847 chr2:11425266(-) A-G intron -    22484847 chr2:11425349(-) A-G intron -    22484847 chr2:11442069(-) A-G intron -    22484847 chr2:11442070(-) A-G intron -    22484847 chr2:11442127(-) A-G intron -    22484847 chr2:11442140(-) A-G intron -    22484847 chr2:11442544(-) A-G intron -    22484847 chr2:11442562(-) A-G intron -    22484847 chr2:11447708(-) A-G intron -    22484847 chr2:11477247(-) A-G intron -    22484847 chr2:11477924(-) A-G intron -    22484847 chr2:11478267(-) A-G intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	ROCK2	chr2:11319898-11319899(-) m6A 3'UTR       27773535 chr2:11320056-11320057(-) m6A 3'UTR       24284625//27773535 chr2:11320163-11320164(-) m6A 3'UTR       24284625 chr2:11320185-11320186(-) m6A 3'UTR       24284625 chr2:11320315-11320316(-) m6A 3'UTR       24284625//24981863//22575960//22608085 chr2:11320337-11320338(-) m6A 3'UTR       24284625//24981863//22575960//22608085 chr2:11320343-11320344(-) m6A 3'UTR       24284625//24981863//22575960//22608085 chr2:11320434-11320435(-) m6A 3'UTR       24284625//24981863 chr2:11321297-11321298(-) m6A 3'UTR       27773535 chr2:11323047-11323048(-) m6A 3'UTR       26404942//27773535 chr2:11323106-11323107(-) m6A 3'UTR       24284625//24981863//26404942//27773535//22608085 chr2:11323236-11323237(-) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//27773535//27371828//22575960//22608085 chr2:11323315-11323316(-) m6A 3'UTR       24284625//24209618//24981863//26404942//27773535//27371828//22575960//22608085 chr2:11323341-11323342(-) m6A 3'UTR       24284625//24209618//24981863//26404942//27773535//22575960//22608085 chr2:11323363-11323364(-) m6A 3'UTR       24284625//24209618//24981863//26404942//27773535//22575960//22608085 chr2:11323451-11323452(-) m6A 3'UTR       24284625//24209618//24981863//26404942//27773535//22575960//22608085 chr2:11323502-11323503(-) m6A 3'UTR       24284625//24209618//24981863//26404942//27773535//22575960//22608085 chr2:11323507-11323508(-) m6A 3'UTR       24284625//24209618//24981863//26404942//27773535//22575960//22608085 chr2:11332278-11332279(-) m6A CDS       24284625//24209618//24981863//27773535//22575960 chr2:11332282-11332283(-) m6A CDS       24284625//24209618//24981863//26404942//27773535//22608085 chr2:11332321-11332322(-) m6A CDS       24284625//24209618//24981863//26404942//27773535//22608085 chr2:11332343-11332344(-) m6A CDS       24284625//24209618//26404942//27773535 chr2:11332369-11332370(-) m6A CDS       24284625//27773535 chr2:11332745-11332746(-) m6A CDS       26404942 chr2:11332802-11332803(-) m6A CDS//exon       24981863//26404942//27773535 chr2:11333951-11333952(-) m6A CDS//exon       24981863//27773535//22575960 chr2:11334413-11334414(-) m6A CDS//exon       26404942 chr2:11334425-11334426(-) m6A CDS//exon       26404942 chr2:11335111-11335112(-) m6A CDS//exon       26404942 chr2:11337415-11337416(-) m6A CDS       24981863//26404942//27773535 chr2:11337424-11337425(-) m6A CDS       24981863//26404942//27773535//22575960 chr2:11337441-11337442(-) m6A CDS       24981863//26404942//27773535//22575960 chr2:11337669-11337670(-) m6A CDS       24981863//26404942//27773535//22575960 chr2:11337792-11337793(-) m6A CDS       24981863//27773535//22575960 chr2:11338621-11338622(-) m6A CDS       24981863//27773535//22575960 chr2:11338630-11338631(-) m6A CDS       24981863//27773535//22575960 chr2:11338881-11338882(-) m6A CDS       24981863//27773535//22575960 chr2:11338890-11338891(-) m6A CDS       24981863//27773535//22575960 chr2:11341112-11341113(-) m6A CDS       24981863//27773535//22575960 chr2:11341118-11341119(-) m6A CDS       24981863//27773535//22575960 chr2:11341124-11341125(-) m6A CDS       24981863//27773535//22575960 chr2:11341165-11341166(-) Am CDS       - chr2:11341190-11341191(-) m6A CDS       24981863//27773535 chr2:11341266-11341267(-) m6A CDS       24981863//27773535 chr2:11341455-11341456(-) m6A CDS       24981863//27773535 chr2:11341482-11341483(-) m6A CDS       24981863//27773535//22575960 chr2:11341487-11341488(-) m6A CDS       24981863//27773535//22575960 chr2:11341526-11341527(-) m6A CDS       24981863//27773535//22575960 chr2:11342189-11342190(-) m6A CDS       24981863//27773535//22575960 chr2:11347859-11347860(-) m6A CDS       24981863//27773535//22575960 chr2:11347871-11347872(-) m6A CDS       24981863//27773535//22575960 chr2:11347881-11347882(-) m6A CDS       24981863//27773535//22575960 chr2:11347960-11347961(-) m6A CDS       24981863//27773535//22608085 chr2:11348002-11348003(-) m6A CDS       24981863//27773535//22608085 chr2:11348028-11348029(-) m6A CDS       24284625//24981863//27773535//22608085 chr2:11348436-11348437(-) m6A CDS       24284625//24981863//27773535//22608085 chr2:11348469-11348470(-) m6A CDS       24284625//24981863//27773535//22608085 chr2:11348482-11348483(-) m6A CDS       24284625//24981863//27773535//22608085 chr2:11348521-11348522(-) m6A CDS       24284625//24981863//27773535//22608085 chr2:11348535-11348536(-) m6A CDS       24284625//24981863//27773535//22608085 chr2:11348543-11348544(-) m6A CDS       24284625//24981863//27773535//22608085 chr2:11351888-11351889(-) m6A CDS       24284625//24981863//27773535//22575960//22608085 chr2:11351903-11351904(-) m6A CDS       24284625//24981863//27773535//22575960//22608085 chr2:11354515-11354516(-) m6A 5'UTR//CDS       24981863//27773535//22575960//22608085 chr2:11354527-11354528(-) m6A 5'UTR//CDS       24981863//27773535//22575960//22608085 chr2:11354998-11354999(-) m6A 5'UTR//CDS       24981863//27773535 chr2:11355056-11355057(-) m6A 5'UTR//CDS       24981863//27773535 chr2:11355070-11355071(-) m6A 5'UTR//CDS       24981863//27773535 chr2:11355084-11355085(-) m6A 5'UTR//CDS       24981863//27773535 chr2:11355131-11355132(-) m6A 5'UTR//CDS       24981863 chr2:11355151-11355152(-) m6A 5'UTR//CDS       24981863 chr2:11355184-11355185(-) m6A 5'UTR//CDS       24981863 chr2:11355202-11355203(-) m6A 5'UTR//CDS       24981863 chr2:11355449-11355450(-) m6A 5'UTR//CDS       24981863 chr2:11355489-11355490(-) m6A 3'UTR//5'UTR//CDS       24981863 chr2:11355661-11355662(-) m6A 3'UTR//5'UTR//CDS       24981863 chr2:11355735-11355736(-) m6A 3'UTR//5'UTR//CDS       24981863//22575960 chr2:11356306-11356307(-) m6A 3'UTR//5'UTR//CDS       24981863//22575960 chr2:11357226-11357227(-) m6A 3'UTR//5'UTR//CDS       24981863//22575960 chr2:11357232-11357233(-) m6A 3'UTR//5'UTR//CDS       24981863//22575960 chr2:11357265-11357266(-) m6A 3'UTR//5'UTR//CDS       24981863//22575960 chr2:11357283-11357284(-) m6A 3'UTR//5'UTR//CDS       24981863//22575960 chr2:11367490-11367491(-) m6A 5'UTR//CDS       22575960 chr2:11484155-11484156(-) m6A 5'UTR//CDS//intron       27773536 chr2:11484216-11484217(-) m6A 5'UTR//CDS//intron       24209618//27371828//27773536 chr2:11484304-11484305(-) m6A 5'UTR//intron       24284625//24209618//27371828//27773536//22575960 chr2:11484399-11484400(-) m6A 5'UTR//intron       24284625//24209618//27371828//27773536//22575960 chr2:11484411-11484412(-) m6A 5'UTR//intron       24284625//24209618//27371828//27773536//22575960 chr2:11484536-11484537(-) m6A 5'UTR//intron       24209618//24981863//27371828//27773536//22575960//22608085 chr2:11484544-11484545(-) m6A 5'UTR//intron       24209618//24981863//27371828//27773536//22575960//22608085 chr2:11484567-11484568(-) m6A 5'UTR//intron       24209618//24981863//27371828//27773536//22575960//22608085 chr2:11488322-11488323(-) m6A exon       24209618//24981863//27371828//27773536//22575960 chr2:11488340-11488341(-) m6A exon       24209618//24981863//22575960

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	ROCK2	Chromatin HCC cell line (HepG2)|K562 cells     - Cytosol Human myelogenous leukemia cell line (K-562)     21613539 Cytosol HCC cell line (HepG2)|K562 cells     - Exosome Blood     - Insoluble cytoplasm HCC cell line (HepG2)     - Membrane HCC cell line (HepG2)|K562 cells     - Nucleoplasm K562 cells     - Nucleus Colon cancer cells     24393600 Nucleus HCC cell line (HepG2)|HeLa-S3 cells|K562 cells     - Ribosome HEK-293 cells     22199352

Related Drug Information:

Resource	Symbol	Drug Name	PubChem ID
RNAactDrug	kshv-miR-K12-4-3p	Panobinostat       6918837 Nilotinib       644241 Irinotecan       60838 Topotecan       60700 l-685,458       5479543 Palbociclib       5330286 Dovitinib       135398510 Crizotinib       11626560 Nvp-aew541       11476171 Pha-665752       10461815

Interaction Network (The top 100 interactions):

Interactor1: kshv-miR-K12-4-3p
Interactor2: ROCK2

Evidence Support:

Strong-Evidence	Luciferase reporter assay//Western blot
Weak-Evidence	PAR-CLIP
Prediction-Evidence	cRep//miRanda//PARalyzer//Rep//Targetscan
Support Database	RepTar//ViRBase//VIRmiRNA//VmiReg

References:

PMID	22100165	Target region	3'UTR
Source	RepTar//ViRBase//VIRmiRNA//VmiReg	Interactor1 expression	None
Tissue or cell line	BC-1 cells	Interactor2 expression	None
Description	Using PAR-CLIP, a technology which allows the direct and transcriptome-wide identification of miRNA targets, we delineate the target sites for all viral and cellular miRNAs expressed in PEL cell lines. The resulting data set revealed that KSHV miRNAs directly target more than 2000 cellular mRNAs, including many involved in pathways relevant to KSHV pathogenesis. Moreover, 58% of these mRNAs are also targeted by EBV miRNAs, via distinct binding sites.Clusters with seed matches to expressed miRNAs were considered candidate target sites. Of the clusters mapping to human 3'UTRs, 69% (BC-1) and 70% (BC-3) had seed matches to expressed miRNAs (Tables S6).Table S6A BC-1 derived 3'UTR clusters with seed matches to expressed miRNAs.