Each method has inherent advantages and limitations, but the combination of these approaches have been successful in identifying many miRNA targets (Table 1). Table 1 List of experimentally validated cellular targets of KSHV- and EBV- encoded miRNAs. The normal cellular functions of these proteins and the effect of their knockdown during viral infection are also listed. The targets that are underlined are repressed by both KSHV- and EBV-encoded miRNAs.
Table S10B Genes that contribute to the enrichments of PAR-CLIP targets of miR-K4-3p from BC-1 libraries in downregulated mRNAs in BJAB/miR-K4-3p cells.Table S10E Genes that contribute to the enrichments of PAR-CLIP targets of miR-K4-3p from BC-3 libraries in downregulated mRNAs in BJAB/miR-K4-3p cells.
Among them, we validated by luciferase reporter assays, quantitative PCR and western blotting caspase 3 (Casp3), a critical factor for the control of apoptosis. Using site-directed mutagenesis, we found that three KSHV miRNAs, miR-K12-1, 3 and 4-3p, were responsible for the targeting of Casp3.
