Basic Information:

  VIRBase ID:  

VHID00450754

  Virus:  

Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)

  Host:  

Homo sapiens

  Confidence Score:  

0.6040

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      kshv-miR-K12-6-5p:  MIMAT0002188          PURA:  NM_005859     

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Interactor Information:

	Interactor1	Interactor2
Symbol	kshv-miR-K12-6-5p	PURA
miRBase Accession/Entrez ID	MIMAT0002188	5813
Organism	Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	MRD31//PUR-ALPHA//PUR1//PURALPHA

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	PURA	chr5:139487519-139487520(+) m6A exon       24284625//25456834//24981863 chr5:139487681-139487682(+) m6A exon       24284625 chr5:139492731-139492732(+) m6A exon//intron       24209618//25456834 chr5:139493773-139493774(+) m6A CDS//exon       24209618//24981863//27773536//22575960 chr5:139493779-139493780(+) m6A CDS//exon       24209618//24981863//27773536//22575960 chr5:139493965-139493966(+) m6A CDS       24284625//24209618//25456834//24981863//22575960//22608085 chr5:139493974-139493975(+) m6A CDS       24284625//24209618//25456834//24981863//22575960//22608085 chr5:139494157-139494158(+) m6A CDS       24284625//24209618//25456834//24981863//22575960//22608085 chr5:139494220-139494221(+) m6A CDS       24284625//25456834//24981863//22575960//22608085 chr5:139494250-139494251(+) m6A CDS       24284625//25456834//24981863//22575960//22608085 chr5:139494324-139494325(+) m6A CDS       24284625//24209618//25456834//24981863//22575960//22608085 chr5:139494445-139494446(+) m6A CDS       24284625//24209618//25456834//24981863//22575960//22608085 chr5:139494569-139494570(+) m6A CDS       24284625//24209618//25456834//24981863//22575960//22608085 chr5:139494603-139494604(+) m1A CDS       26863196 chr5:139494681-139494682(+) m6A CDS       24284625//24209618//25456834//24981863//27773535//22575960//22608085 chr5:139494718-139494719(+) m1A CDS       26863196 chr5:139494727-139494728(+) m1A CDS       26863196 chr5:139494739-139494740(+) m6A 3'UTR       24284625//24209618//25456834//24981863//27773535//22575960//22608085 chr5:139494749-139494750(+) m6A 3'UTR       24284625//24209618//25456834//24981863//27773535//22575960//22608085 chr5:139494860-139494861(+) m6A 3'UTR       24284625//24209618//25456834//24981863//27773535//22575960//22608085 chr5:139494893-139494894(+) m6A 3'UTR       24284625//24209618//24981863//27773535//22575960//22608085 chr5:139494970-139494971(+) m6A 3'UTR       24284625//24209618//24981863//27773535//22575960//22608085 chr5:139494990-139494991(+) m6A 3'UTR       24284625//24209618//24981863//27773535//22575960//22608085 chr5:139495025-139495026(+) m6A 3'UTR       24284625//24209618//24981863//27773535//22608085 chr5:139495059-139495060(+) m6A 3'UTR       24284625//24209618//24981863//22608085 chr5:139495090-139495091(+) m6A 3'UTR       24284625//24981863//22608085

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	PURA	Cytosol Human myelogenous leukemia cell line (K-562)     21613539 Exosome Blood     - Ribosome HEK-293 cells     22199352

Related Drug Information:

Resource	Symbol	Drug Name	PubChem ID
RNAactDrug	kshv-miR-K12-6-5p	l-685,458       5479543

Interaction Network (The top 100 interactions):

Interactor1: kshv-miR-K12-6-5p
Interactor2: PURA

Evidence Support:

Weak-Evidence	PAR-CLIP
Prediction-Evidence	miRanda//PARalyzer//Rep//Targetscan
Support Database	RepTar//ViRBase//VIRmiRNA//VmiReg

References:

PMID	22100165	Target region	3'UTR
Source	RepTar//ViRBase//VIRmiRNA//VmiReg	Interactor1 expression	None
Tissue or cell line	BC-1 cells	Interactor2 expression	None
Description	Using PAR-CLIP, a technology which allows the direct and transcriptome-wide identification of miRNA targets, we delineate the target sites for all viral and cellular miRNAs expressed in PEL cell lines. The resulting data set revealed that KSHV miRNAs directly target more than 2000 cellular mRNAs, including many involved in pathways relevant to KSHV pathogenesis. Moreover, 58% of these mRNAs are also targeted by EBV miRNAs, via distinct binding sites.Clusters with seed matches to expressed miRNAs were considered candidate target sites. Of the clusters mapping to human 3'UTRs, 69% (BC-1) and 70% (BC-3) had seed matches to expressed miRNAs (Tables S6).Table S6A BC-1 derived 3'UTR clusters with seed matches to expressed miRNAs.