Basic Information:

  VIRBase ID:  

VHID00450418

  Virus:  

Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)

  Host:  

Homo sapiens

  Confidence Score:  

0.5908

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      kshv-miR-K12-6-5p:  MIMAT0002188          KRAS:  NM_001369787     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	kshv-miR-K12-6-5p	KRAS
miRBase Accession/Entrez ID	MIMAT0002188	3845
Organism	Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	'C-K-RAS//C-K-RAS//c-Ki-ras//c-Ki-ras2//CFC2//K-Ras//K-Ras 2//K-RAS2A//K-RAS2B//K-RAS4A//K-RAS4B//KI-RAS//KRAS1//KRAS2//NS//NS3//OES//RALD//RASK2

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	KRAS	chr12:25364397(-) A-I      intronic chr12:25384258(-) A-I      intronic chr12:25384200(-) A-I      intronic chr12:25372046(-) A-I      intronic chr12:25384290(-) A-I      intronic chr12:25374360(-) A-I      intronic chr12:25384190(-) A-I      intronic chr12:25374361(-) A-I      intronic chr12:25384240(-) A-I      intronic chr12:25374327(-) A-I      intronic chr12:25372011(-) A-I      intronic chr12:25374275(-) A-I      intronic chr12:25384234(-) A-I      intronic chr12:25372052(-) A-I      intronic chr12:25372051(-) A-I      intronic chr12:25372048(-) A-I      intronic chr12:25372037(-) A-I      intronic chr12:25372042(-) A-I      intronic chr12:25372043(-) A-I      intronic chr12:25383424(-) A-I      intronic chr12:25383462(-) A-I      intronic chr12:25374331(-) A-I      intronic chr12:25383413(-) A-I      intronic chr12:25383360(-) A-I      intronic chr12:25382978(-) A-I      intronic chr12:25382867(-) A-I      intronic chr12:25382827(-) A-I      intronic chr12:25381858(-) A-I      intronic chr12:25374975(-) A-I      intronic chr12:25384132(-) A-I      intronic chr12:25401045(-) A-I      intronic chr12:25401203(-) A-I      intronic chr12:25365438(-) A-I      intronic chr12:25374971(-) A-I      intronic chr12:25383515(-) A-I      intronic chr12:25370746(-) A-I      intronic chr12:25381830(-) A-I      intronic chr12:25372001(-) A-I      intronic chr12:25374258(-) A-I      intronic chr12:25383499(-) A-I      intronic chr12:25374264(-) A-I      intronic chr12:25381750(-) A-I      intronic chr12:25382964(-) A-I      intronic chr12:25382915(-) A-I      intronic chr12:25374961(-) A-I      intronic chr12:25370728(-) A-I      intronic chr12:25383418(-) A-I      intronic chr12:25382779(-) A-I      intronic chr12:25383510(-) A-I      intronic chr12:25402183(-) A-I      intronic chr12:25369459(-) A-I      intronic chr12:25399380(-) A-I      intronic chr12:25374252(-) A-I      intronic chr12:25370742(-) A-I      intronic chr12:25365781(-) A-I      intronic chr12:25381735(-) A-I      intronic chr12:25371969(-) A-I      intronic chr12:25370776(-) A-I      intronic chr12:25374400(-) A-I      intronic chr12:25374342(-) A-I      intronic chr12:25383306(-) A-I      intronic chr12:25374234(-) A-I      intronic chr12:25383493(-) A-I      intronic chr12:25383365(-) A-I      intronic chr12:25369311(-) A-I      intronic chr12:25374263(-) A-I      intronic chr12:25384160(-) A-I      intronic chr12:25365830(-) A-I      intronic chr12:25369468(-) A-I      intronic chr12:25365829(-) A-I      intronic chr12:25374304(-) A-I      intronic chr12:25364479(-) A-I      intronic chr12:25374395(-) A-I      intronic chr12:25365845(-) A-I      intronic chr12:25365441(-) A-I      intronic chr12:25365803(-) A-I      intronic chr12:25365735(-) A-I      intronic chr12:25382886(-) A-I      intronic chr12:25384213(-) A-I      intronic chr12:25382928(-) A-I      intronic chr12:25384174(-) A-I      intronic chr12:25374256(-) A-I      intronic chr12:25374423(-) A-I      intronic chr12:25383480(-) A-I      intronic chr12:25384289(-) A-I      intronic chr12:25365741(-) A-I      intronic chr12:25381825(-) A-I      intronic chr12:25373896(-) A-I      intronic chr12:25374290(-) A-I      intronic chr12:25365415(-) A-I      intronic chr12:25365746(-) A-I      intronic chr12:25370828(-) A-I      intronic chr12:25382935(-) A-I      intronic chr12:25383479(-) A-I      intronic chr12:25374935(-) A-I      intronic chr12:25374371(-) A-I      intronic chr12:25369347(-) A-I      intronic chr12:25384184(-) A-I      intronic chr12:25374340(-) A-I      intronic chr12:25374895(-) A-I      intronic chr12:25401160(-) A-I      intronic chr12:25384248(-) A-I      intronic chr12:25381776(-) A-I      intronic chr12:25374372(-) A-I      intronic chr12:25364422(-) A-I      intronic chr12:25401321(-) A-I      intronic chr12:25383557(-) A-I      intronic chr12:25383559(-) A-I      intronic chr12:25374295(-) A-I      intronic chr12:25372145(-) A-I      intronic chr12:25401161(-) A-I      intronic chr12:25382940(-) A-I      intronic

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	KRAS	chr12:25365741(-) A-I intron -    21960545   //22327324 chr12:25365781(-) A-I intron -    21960545   //22327324 chr12:25365415(-) A-I intron -    21960545 chr12:25365416(-) A-I intron -    21960545 chr12:25365829(-) A-I intron -    21960545 chr12:25365830(-) A-I intron -    21960545 chr12:25365833(-) A-I intron -    21960545 chr12:25369307(-) A-I intron -    21960545 chr12:25369341(-) A-I intron -    21960545 chr12:25369347(-) A-I intron -    21960545 chr12:25370623(-) A-I intron -    21960545 chr12:25370728(-) A-I intron -    21960545 chr12:25370746(-) A-I intron -    21960545 chr12:25370776(-) A-I intron -    21960545 chr12:25370828(-) A-I intron -    21960545 chr12:25382901(-) A-I intron -    21960545 chr12:25383306(-) A-I intron -    21960545 chr12:25383424(-) A-I intron -    21960545 chr12:25383557(-) A-I intron -    21960545 chr12:25360448(-) G-T exon 3'UTR    22484847 chr12:25360449(-) G-T exon 3'UTR    22484847 chr12:25365741(-) A-G intron -    22484847 chr12:25365781(-) A-G intron -    22484847 chr12:25365958(-) T-C intron -    22484847 chr12:25374244(-) T-C intron -    22484847 chr12:25382928(-) A-G intron -    22484847 chr12:25382935(-) A-G intron -    22484847 chr12:25383480(-) A-G intron -    22484847 chr12:25369303(-) T-C intron -    22484847 chr12:25370828(-) A-G intron -    22484847 chr12:25372145(-) A-G intron -    22484847 chr12:25374395(-) A-G intron -    22484847 chr12:25374400(-) A-G intron -    22484847 chr12:25374423(-) A-G intron -    22484847 chr12:25374895(-) A-G intron -    22484847 chr12:25382780(-) T-C intron -    22484847 chr12:25383365(-) A-G intron -    22484847 chr12:25383479(-) A-G intron -    22484847 chr12:25383493(-) A-G intron -    22484847 chr12:25383510(-) A-G intron -    22484847 chr12:25384160(-) A-G intron -    22484847 chr12:25384184(-) A-G intron -    22484847 chr12:25401160(-) A-G intron -    22484847 chr12:25401161(-) A-G intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	KRAS	chr12:25362748-25362749(-) m6A 3'UTR//CDS       24981863//27773535 chr12:25362800-25362801(-) m6A 3'UTR//CDS       24981863//27773535//27371828 chr12:25368415-25368416(-) m6A CDS//intron       27773535//27371828 chr12:25378550-25378551(-) m6A CDS//intron       24981863//27773535//27371828//22608085 chr12:25378555-25378556(-) m6A CDS//intron       24981863//27773535//27371828//22608085 chr12:25378567-25378568(-) m6A CDS//intron       24981863//27773535//27371828//22608085 chr12:25378602-25378603(-) m6A CDS//intron       24981863//27773535//27371828//22608085 chr12:25378613-25378614(-) m6A CDS//intron       24981863//27773535//27371828//22608085 chr12:25378620-25378621(-) m6A CDS//intron       24981863//27773535//27371828//22608085 chr12:25378627-25378628(-) m6A CDS//intron       24981863//27773535//27371828//22608085 chr12:25378683-25378684(-) m6A CDS//intron       24981863//27773535//27371828//22608085 chr12:25378703-25378704(-) m6A CDS//intron       24981863//27773535//27371828//22608085 chr12:25380237-25380238(-) m6A CDS//intron       24981863//26404942//27773535 chr12:25380251-25380252(-) m6A CDS//intron       24981863//26404942//27773535 chr12:25380309-25380310(-) m6A CDS//intron       24981863//26404942//27773535 chr12:25387487-25387488(-) m6A 3'UTR//intron       27773535 chr12:25387524-25387525(-) m6A 3'UTR//intron       27773535 chr12:25398303-25398304(-) m6A CDS       24209618//27371828 chr12:25403761-25403762(-) m6A 5'UTR       24209618//24981863//27371828

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	KRAS	Chromatin K562 cells     - Exosome Serum     - Mitochondrion HDF cells     16178658 Nucleoplasm K562 cells     - Nucleus K562 cells     - Ribosome Colon cancer cells     24393600

Related Drug Information:

Resource	Symbol	Drug Name	PubChem ID
RNAactDrug	kshv-miR-K12-6-5p	l-685,458       5479543

Interaction Network (The top 100 interactions):

Interactor1: kshv-miR-K12-6-5p
Interactor2: KRAS

Evidence Support:

Weak-Evidence	Microarray
Prediction-Evidence	miRanda//Targetscan
Support Database	ViRBase//VmiReg

References:

PMID	22174674	Target region	None
Source	ViRBase//VmiReg	Interactor1 expression	None
Tissue or cell line	EA.hy926 cells	Interactor2 expression	Downregulation
Description	As KSHV putative direct targets we extracted transcripts that were significantly down-regulated significantly in the replicate experiments, and which contained at least one seed-match to one of the KSHV miRNAs. We identified 704 putative direct targets in DG-75 cells (Figure 3B), and 980 putative direct targets in EA.hy926 cells (Figure 3C). A complete list of putative direct targets can be found in Dataset S2 for DG-75 cells and in Dataset S3 for EA.hy926 cells. The overlap between the two datasets contained 153 putative direct targets (Dataset S4).