Basic Information:

  VIRBase ID:  

VHID00447466

  Virus:  

Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)

  Host:  

Homo sapiens

  Confidence Score:  

0.9942

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      kshv-miR-K12-7-3p:  MIMAT0002187          MICB:  NM_001289160     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	kshv-miR-K12-7-3p	MICB
miRBase Accession/Entrez ID	MIMAT0002187	4277
Organism	Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	PERB11.2

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	MICB	chr6:31471991(+) A-I      intronic chr6:31470045(+) A-I      intronic chr6:31470010(+) A-I      intronic chr6:31472130(+) A-I      intronic chr6:31469983(+) A-I      intronic chr6:31470008(+) A-I      intronic chr6:31470005(+) A-I      intronic chr6:31472098(+) A-I      intronic chr6:31469992(+) A-I      intronic chr6:31472092(+) A-I      intronic chr6:31469911(+) A-I      intronic

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	MICB	chr6:31473469-31473470(+) m6A CDS       - chr6:31473532-31473533(+) m6A CDS       25456834 chr6:31473563-31473564(+) m6A CDS       25456834 chr6:31473570-31473571(+) m6A CDS       25456834 chr6:31473578-31473579(+) m6A CDS       25456834 chr6:31473585-31473586(+) m6A CDS       24284625//25456834//24981863 chr6:31473606-31473607(+) m6A CDS       24284625//25456834//24981863 chr6:31473617-31473618(+) m6A CDS       24284625//25456834//24981863 chr6:31473636-31473637(+) m6A CDS       24284625//25456834//24981863 chr6:31473715-31473716(+) m6A exon//intron       24284625//25456834//24981863 chr6:31478073-31478074(+) m6A 3'UTR       24981863

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	kshv-miR-K12-7-3p	Exosome Jurkat-derived T cell line (J77cl20)     21505438
	MICB	Cytosol HCC cell line (HepG2)     - Exosome Blood     - Nucleoplasm K562 cells     - Nucleus HeLa-S3 cells     - Ribosome Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: kshv-miR-K12-7-3p
Interactor2: MICB

Evidence Support:

Strong-Evidence	Dual luciferase reporter assay//Luciferase reporter assay//qRT-PCR//Western blot
Weak-Evidence	PAR-CLIP//RIP-Chip
Prediction-Evidence	cRep//miRanda//PARalyzer//PITA//RNAHybrid//Targetscan
Support Database	RepTar//ViRBase//VIRmiRNA//VmiReg

References:

[1]PMID	20580307	Target region	3'UTR
Source	RepTar//ViRBase	Interactor1 expression	None
Tissue or cell line	293T cells//RKO cells	Interactor2 expression	Downregulation
Description	From the targets identified to date (Table 1, Figure 1) it is apparent that viral miRNAs play an important role in immune evasion by inhibiting immune surveillance and extending the life of the infected host cell. Table 1 Immunomodulatory viral miRNAs.

[2]PMID	21277611	Target region	3'UTR
Source	RepTar//ViRBase	Interactor1 expression	None
Tissue or cell line	293T cells//RKO cells	Interactor2 expression	Downregulation
Description	In a surprising twist, by systematic functional testing of EBV and KSHV miRNAs, Nachmani et al. later showed that EBV as well as KSHV also express miRNAs with the ability to suppress MICB expression (Nachmani et al., 2009).

[3]PMID	23170179	Target region	3'UTR
Source	RepTar//ViRBase	Interactor1 expression	None
Tissue or cell line	293T cells//RKO cells	Interactor2 expression	Downregulation
Description	The major histocompatibility complex class I-related chain B (MICB) is a stress-induced protein recognized by the NKG2D receptor on NK-cells and CD8+ T-lymphocytes. Interaction between MICB and NKG2D generates a cytolytic response by NK- and T-cells. Nachmani et.al. demonstrated that the 3'UTR of MICB is targeted by EBV miRNA, BART2-5p and by the KSHV miRNA, miR-K12-7.

[4]PMID	19380116	Target region	3'UTR
Source	RepTar//ViRBase//VIRmiRNA//VmiReg	Interactor1 expression	None
Tissue or cell line	293T cells//RKO cells	Interactor2 expression	Downregulation
Description	Here, we show functional conservation among diverse microRNAs derived from different herpesviruses, including HCMV, Kaposi's sarcoma-associated herpesvirus (KSHV), and Epstein-Barr virus (EBV), in their ability to directly target MICB mRNA and reduce its expression. Although the various viral microRNAs share no sequence homology, they are functionally similar and target MICB at different yet adjacent sites during authentic viral infection.

[5]PMID	20413099	Target region	3'UTR
Source	RepTar//ViRBase//VIRmiRNA//VmiReg	Interactor1 expression	None
Tissue or cell line	293 cells//BCBL-1 cells//BL41 cells//DG75 cells//HEK293 cells//Jijoye cells	Interactor2 expression	Downregulation
Description	Recently, the activating NK cell ligand MICB was found to be targeted by both ebv-miR-BART2-5p, kshv-miR-K12-7 and hcmvmiR-UL112-1.

[6]PMID	21616184	Target region	3'UTR
Source	RepTar//ViRBase//VIRmiRNA//VmiReg	Interactor1 expression	None
Tissue or cell line	293T cells//RKO cells	Interactor2 expression	Downregulation
Description	Flow analysis of 293 cells transfected with KSHV miRNAs revealed miR-K12-7 inhibited MICB protein expression, but mRNA levels were not significantly changed. MICB is a surface marker that can be upregulated by viral infection and recognized by NK cells.

[7]PMID	22100165	Target region	3'UTR
Source	RepTar//ViRBase//VIRmiRNA//VmiReg	Interactor1 expression	None
Tissue or cell line	PEL cells	Interactor2 expression	Downregulation
Description	Our dataset also confirmed 12 out of 29 validated KSHV miRNA targets with expression in our dataset(>40%). Confirmed interactions include those with BACH1, FOS, CDKN1A(p21), TNFRSF12A(TWEAKR), RAD21 and RBL2 mRNAs, whose regulation had previously been validated at the level of protein expression. A list summarizing the recovery of previously published targets of the KSHV and EBV miRNAs is presented in Table S9. NIHMS333942-supplement-01 Table S9 Recovery of previously published targets for KSHV or EBV miRNAs.