Basic Information:

  VIRBase ID:  

VHID00447132

  Virus:  

Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)

  Host:  

Homo sapiens

  Confidence Score:  

0.8925

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      kshv-miR-K12-7-3p:  MIMAT0002187          BNIP3:  NM_004052     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	kshv-miR-K12-7-3p	BNIP3
miRBase Accession/Entrez ID	MIMAT0002187	664
Organism	Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	NIP3

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	BNIP3	chr10:133789718(-) A-I      intronic chr10:133786663(-) A-I      intronic chr10:133790597(-) A-I      intronic chr10:133790651(-) A-I      intronic chr10:133788139(-) A-I      intronic chr10:133788083(-) A-I      intronic chr10:133788030(-) A-I      intronic chr10:133790690(-) A-I      intronic chr10:133788046(-) A-I      intronic chr10:133790533(-) A-I      intronic chr10:133787973(-) A-I      intronic chr10:133790661(-) A-I      intronic chr10:133788156(-) A-I      intronic chr10:133787970(-) A-I      intronic chr10:133790614(-) A-I      intronic chr10:133788157(-) A-I      intronic chr10:133790372(-) A-I      intronic

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	BNIP3	chr10:133788046(-) A-I intron -    22327324 chr10:133787970(-) A-G intron -    22484847 chr10:133788046(-) A-G intron -    22484847 chr10:133788083(-) A-G intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	BNIP3	chr10:133780036-133780037(-) m6A 3'UTR       - chr10:133780047-133780048(-) m6A 3'UTR       - chr10:133780062-133780063(-) m6A 3'UTR       - chr10:133780073-133780074(-) m6A 3'UTR       - chr10:133780090-133780091(-) m6A 3'UTR       - chr10:133780173-133780174(-) m6A 3'UTR       - chr10:133782327-133782328(-) m6A 3'UTR//intron       - chr10:133782353-133782354(-) m6A 3'UTR//intron       - chr10:133782406-133782407(-) m6A 3'UTR//intron       - chr10:133782468-133782469(-) m6A 3'UTR//intron       - chr10:133782474-133782475(-) m6A 3'UTR//intron       - chr10:133782493-133782494(-) m6A 3'UTR//intron       - chr10:133783080-133783081(-) m6A 3'UTR//intron       - chr10:133783184-133783185(-) m6A 3'UTR//intron       - chr10:133783197-133783198(-) m6A 3'UTR//intron       - chr10:133783225-133783226(-) m6A 3'UTR//intron       - chr10:133783737-133783738(-) m6A 3'UTR//intron       24284625 chr10:133783742-133783743(-) m6A 3'UTR//intron       24284625 chr10:133783983-133783984(-) m6A 3'UTR//intron       24284625//25456834 chr10:133784014-133784015(-) m6A 3'UTR//intron       24284625//25456834 chr10:133784240-133784241(-) m6A CDS       27773535 chr10:133784275-133784276(-) m6A CDS       27773535 chr10:133784418-133784419(-) m6A CDS       24981863//28052920//27773535 chr10:133786547-133786548(-) m6A CDS       24981863//27773535 chr10:133786575-133786576(-) m6A CDS       24981863//27773535 chr10:133786605-133786606(-) m6A CDS       24981863//27773535 chr10:133787368-133787369(-) m6A CDS//exon       24981863//27773535 chr10:133787433-133787434(-) m6A CDS//exon       27773535//27371828 chr10:133795340-133795341(-) m6A CDS//exon       24981863//27371828//27773536

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	kshv-miR-K12-7-3p	Exosome Jurkat-derived T cell line (J77cl20)     21505438
	BNIP3	Chromatin K562 cells     - Endoplasmic reticulum Human fibrosarcoma cells (HT1080)     25753659 Exosome Blood     - Membrane HCC cell line (HepG2)     - Nucleolus K562 cells     - Nucleoplasm K562 cells     - Nucleus HCC cell line (HepG2)|HeLa-S3 cells|K562 cells     - Ribosome Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: kshv-miR-K12-7-3p
Interactor2: BNIP3

Evidence Support:

Strong-Evidence	3'UTR indicator assay
Weak-Evidence	PAR-CLIP
Prediction-Evidence	miRanda//PARalyzer//Targetscan
Support Database	ViRBase//VmiReg

References:

PMID	22100165	Target region	3'UTR
Source	ViRBase//VmiReg	Interactor1 expression	None
Tissue or cell line	BC-3 cells	Interactor2 expression	None
Description	We focused this analysis mostly on 3'UTRs of mRNAs encoding proteins with functions of potential relevance to KSHV biology and pathogenesis (Table S11). Thirty-six combinations of 3'UTR indicators and KSHV miRNAs with PAR-CLIP interactions were tested for regulation. Nine of these interactions were independently also predicted by microarray analysis of the BJAB cell pools expressing miR-K1, miR-K4-3p, or miR-K11 described above (i.e. for miR-K1: NMI, RAD21, BCL11A, RFXAP; for miR-K4-3p: TPD52, GRB2, MCC, YWHAB; for miR-K11: WEE1). Targets were representative of the full range of seed match types observed in the libraries (Tables S6 and S11).