Basic Information:

  VIRBase ID:  

VHID00446814

  Virus:  

Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)

  Host:  

Homo sapiens

  Confidence Score:  

0.5908

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      kshv-miR-K12-8-3p:  MIMAT0002186          RNF25:  NM_022453     

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Interactor Information:

	Interactor1	Interactor2
Symbol	kshv-miR-K12-8-3p	RNF25
miRBase Accession/Entrez ID	MIMAT0002186	64320
Organism	Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	AO7

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	RNF25	chr2:219531815(-) A-I      intronic chr2:219531839(-) A-I      intronic chr2:219531869(-) A-I      intronic chr2:219531775(-) A-I      intronic chr2:219532142(-) A-I      intronic chr2:219532170(-) A-I      intronic

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	RNF25	chr2:219531869(-) A-G intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	RNF25	chr2:219528660-219528661(-) m6A 3'UTR//exon       24209618//25456834//24981863//27773535//27371828//27773536//22608085 chr2:219528674-219528675(-) m6A 3'UTR//exon       24209618//25456834//24981863//27773535//27371828//27773536//22608085 chr2:219528780-219528781(-) m6A CDS//exon       24284625//24209618//25456834//24981863//27773535//27371828//27773536//22575960//22608085 chr2:219528812-219528813(-) m6A CDS//exon       24284625//24209618//25456834//24981863//27773535//27371828//27773536//22575960//22608085 chr2:219528819-219528820(-) m6A CDS//exon       24284625//24209618//25456834//24981863//27773535//27371828//27773536//22575960//22608085 chr2:219528895-219528896(-) m6A CDS//exon       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//27773536//22575960//22608085 chr2:219528908-219528909(-) m6A CDS//exon       24284625//24209618//25456834//24981863//28052920//26404942//27773535//27371828//27773536//22575960//22608085 chr2:219528946-219528947(-) m6A CDS//exon       24284625//24209618//25456834//24981863//26404942//27773535//27371828//27773536//22575960//22608085 chr2:219529065-219529066(-) m6A CDS//exon       24284625//24209618//25456834//24981863//27773535//27371828//27773536//22575960//22608085 chr2:219529095-219529096(-) m6A CDS//exon       24284625//24209618//24981863//27773535//27371828 chr2:219529180-219529181(-) m6A CDS//exon       24284625//24981863//27773535//27371828 chr2:219529240-219529241(-) m6A CDS//exon       24284625//24981863//27773535//27371828 chr2:219529486-219529487(-) m6A CDS//exon       24284625//24981863//27773535//27371828 chr2:219529892-219529893(-) m6A 3'UTR//CDS//exon       27773535//27371828 chr2:219530641-219530642(-) m6A 3'UTR//CDS//exon       27773535//27371828 chr2:219530668-219530669(-) m6A 3'UTR//CDS//exon       27773535//27371828 chr2:219530680-219530681(-) m6A 3'UTR//CDS//exon       27773535//27371828 chr2:219536655-219536656(-) m6A CDS//exon       24981863 chr2:219536834-219536835(-) m6A 5'UTR       24981863 chr2:219536844-219536845(-) m6A 5'UTR       24981863 chr2:219536867-219536868(-) m6A 5'UTR       24981863 chr2:219536901-219536902(-) m6A 5'UTR       24981863 chr2:219536921-219536922(-) m6A 5'UTR       24981863

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	kshv-miR-K12-8-3p	Exosome Jurkat-derived T cell line (J77cl20)     21505438
	RNF25	Cytosol Human myelogenous leukemia cell line (K-562)     21613539 Exosome Blood     - Nucleus K562 cells     - Ribosome Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: kshv-miR-K12-8-3p
Interactor2: RNF25

Evidence Support:

Weak-Evidence	Microarray
Prediction-Evidence	miRanda//Targetscan
Support Database	ViRBase//VmiReg

References:

PMID	22174674	Target region	None
Source	ViRBase//VmiReg	Interactor1 expression	None
Tissue or cell line	EA.hy926 cells	Interactor2 expression	Downregulation
Description	As KSHV putative direct targets we extracted transcripts that were significantly down-regulated significantly in the replicate experiments, and which contained at least one seed-match to one of the KSHV miRNAs. We identified 704 putative direct targets in DG-75 cells (Figure 3B), and 980 putative direct targets in EA.hy926 cells (Figure 3C). A complete list of putative direct targets can be found in Dataset S2 for DG-75 cells and in Dataset S3 for EA.hy926 cells. The overlap between the two datasets contained 153 putative direct targets (Dataset S4).