Basic Information:

  VIRBase ID:  

VHID00446753

  Virus:  

Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)

  Host:  

Homo sapiens

  Confidence Score:  

0.6002

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      kshv-miR-K12-8-3p:  MIMAT0002186          RFWD3:  NM_001370542     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	kshv-miR-K12-8-3p	RFWD3
miRBase Accession/Entrez ID	MIMAT0002186	55159
Organism	Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	FANCW//RNF201

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	RFWD3	chr16:74659326(-) A-I      intronic chr16:74664308(-) A-I      intronic chr16:74685422(-) A-I      intronic chr16:74658522(-) A-I      intronic chr16:74680615(-) A-I      intronic chr16:74676294(-) A-I      intronic chr16:74676264(-) A-I      intronic chr16:74676193(-) A-I      intronic chr16:74676159(-) A-I      intronic chr16:74675768(-) A-I      intronic chr16:74675715(-) A-I      intronic chr16:74675649(-) A-I      intronic chr16:74675641(-) A-I      intronic chr16:74675249(-) A-I      intronic chr16:74675209(-) A-I      intronic chr16:74675205(-) A-I      intronic chr16:74675199(-) A-I      intronic chr16:74687169(-) A-I      intronic chr16:74674785(-) A-I      intronic chr16:74674777(-) A-I      intronic chr16:74658462(-) A-I      intronic chr16:74658379(-) A-I      intronic chr16:74658361(-) A-I      intronic chr16:74674754(-) A-I      intronic chr16:74674699(-) A-I      intronic chr16:74682897(-) A-I      intronic chr16:74681717(-) A-I      intronic chr16:74681614(-) A-I      intronic chr16:74680789(-) A-I      intronic chr16:74680777(-) A-I      intronic chr16:74682880(-) A-I      intronic chr16:74682558(-) A-I      intronic chr16:74682317(-) A-I      intronic chr16:74682254(-) A-I      intronic chr16:74674679(-) A-I      intronic chr16:74674226(-) A-I      intronic chr16:74681981(-) A-I      intronic chr16:74681913(-) A-I      intronic chr16:74674631(-) A-I      intronic chr16:74674298(-) A-I      intronic chr16:74680501(-) A-I      intronic chr16:74680492(-) A-I      intronic chr16:74679897(-) A-I      intronic chr16:74674279(-) A-I      intronic chr16:74679890(-) A-I      intronic chr16:74679824(-) A-I      intronic chr16:74679783(-) A-I      intronic chr16:74689214(-) A-I      intronic chr16:74682927(-) A-I      intronic chr16:74682930(-) A-I      intronic chr16:74682971(-) A-I      intronic chr16:74685259(-) A-I      intronic chr16:74685286(-) A-I      intronic chr16:74686774(-) A-I      intronic chr16:74687129(-) A-I      intronic chr16:74674109(-) A-I      intronic chr16:74671381(-) A-I      intronic chr16:74680560(-) A-I      intronic chr16:74671345(-) A-I      intronic chr16:74670718(-) A-I      intronic chr16:74690598(-) A-I      intronic chr16:74692855(-) A-I      intronic chr16:74693441(-) A-I      intronic chr16:74694239(-) A-I      intronic chr16:74694251(-) A-I      intronic chr16:74694269(-) A-I      intronic chr16:74696940(-) A-I      intronic chr16:74696941(-) A-I      intronic chr16:74669282(-) A-I      intronic chr16:74668989(-) A-I      intronic chr16:74668792(-) A-I      intronic chr16:74668559(-) A-I      intronic chr16:74699852(-) A-I      intronic chr16:74699413(-) A-I      intronic chr16:74699393(-) A-I      intronic chr16:74699389(-) A-I      intronic chr16:74699264(-) A-I      intronic chr16:74699152(-) A-I      intronic chr16:74667818(-) A-I      intronic chr16:74667699(-) A-I      intronic chr16:74667663(-) A-I      intronic chr16:74667643(-) A-I      intronic chr16:74667630(-) A-I      intronic chr16:74687142(-) A-I      intronic chr16:74687154(-) A-I      intronic chr16:74687161(-) A-I      intronic chr16:74667613(-) A-I      intronic chr16:74682870(-) A-I      intronic chr16:74658386(-) A-I      intronic chr16:74667129(-) A-I      intronic chr16:74666190(-) A-I      intronic chr16:74665199(-) A-I      intronic chr16:74665179(-) A-I      intronic chr16:74665147(-) A-I      intronic chr16:74665125(-) A-I      intronic chr16:74664201(-) A-I      intronic chr16:74663479(-) A-I      intronic chr16:74663354(-) A-I      intronic chr16:74659038(-) A-I      intronic chr16:74659029(-) A-I      intronic chr16:74679705(-) A-I      intronic chr16:74676680(-) A-I      intronic chr16:74676663(-) A-I      intronic chr16:74676467(-) A-I      intronic chr16:74658830(-) A-I      intronic chr16:74658750(-) A-I      intronic chr16:74658691(-) A-I      intronic chr16:74656306(-) A-I      3'UTR chr16:74656266(-) A-I      3'UTR chr16:74675356(-) A-I      intronic chr16:74675778(-) A-I      intronic chr16:74682484(-) A-I      intronic chr16:74679856(-) A-I      intronic chr16:74663511(-) A-I      intronic chr16:74673084(-) A-I      intronic chr16:74681983(-) A-I      intronic chr16:74658637(-) A-I      intronic chr16:74670773(-) A-I      intronic chr16:74682621(-) A-I      intronic chr16:74675762(-) A-I      intronic chr16:74682798(-) A-I      intronic chr16:74675118(-) A-I      intronic chr16:74680521(-) A-I      intronic chr16:74671328(-) A-I      intronic chr16:74691538(-) A-I      intronic chr16:74681694(-) A-I      intronic chr16:74675653(-) A-I      intronic chr16:74670804(-) A-I      intronic chr16:74679530(-) A-I      intronic chr16:74694349(-) A-I      intronic chr16:74668380(-) A-I      intronic chr16:74664221(-) A-I      intronic chr16:74677151(-) A-I      intronic chr16:74666099(-) A-I      intronic chr16:74664277(-) A-I      intronic chr16:74669351(-) A-I      intronic chr16:74690456(-) A-I      intronic chr16:74659921(-) A-I      intronic chr16:74681709(-) A-I      intronic chr16:74658649(-) A-I      intronic chr16:74681277(-) A-I      intronic chr16:74675748(-) A-I      intronic chr16:74687011(-) A-I      intronic chr16:74675146(-) A-I      intronic chr16:74667788(-) A-I      intronic chr16:74667128(-) A-I      intronic chr16:74670807(-) A-I      intronic chr16:74690168(-) A-I      intronic chr16:74681630(-) A-I      intronic chr16:74694307(-) A-I      intronic chr16:74682896(-) A-I      intronic chr16:74663377(-) A-I      intronic chr16:74685460(-) A-I      intronic chr16:74687054(-) A-I      intronic chr16:74668537(-) A-I      intronic chr16:74671118(-) A-I      intronic chr16:74671449(-) A-I      intronic chr16:74682885(-) A-I      intronic chr16:74676094(-) A-I      intronic chr16:74676218(-) A-I      intronic chr16:74681706(-) A-I      intronic chr16:74682584(-) A-I      intronic chr16:74699470(-) A-I      intronic chr16:74699868(-) A-I      intronic chr16:74664193(-) A-I      intronic chr16:74690494(-) A-I      intronic chr16:74698135(-) A-I      intronic chr16:74656355(-) A-I      3'UTR chr16:74656358(-) A-I      3'UTR chr16:74681646(-) A-I      intronic chr16:74690538(-) A-I      intronic chr16:74675765(-) A-I      intronic chr16:74658458(-) A-I      intronic chr16:74686980(-) A-I      intronic chr16:74675334(-) A-I      intronic chr16:74682928(-) A-I      intronic chr16:74671403(-) A-I      intronic chr16:74675319(-) A-I      intronic chr16:74673215(-) A-I      intronic chr16:74699563(-) A-I      intronic chr16:74681947(-) A-I      intronic chr16:74670814(-) A-I      intronic chr16:74681971(-) A-I      intronic chr16:74664171(-) A-I      intronic chr16:74682575(-) A-I      intronic chr16:74681595(-) A-I      intronic chr16:74681379(-) A-I      intronic chr16:74675087(-) A-I      intronic chr16:74682949(-) A-I      intronic chr16:74679852(-) A-I      intronic chr16:74674435(-) A-I      intronic chr16:74682588(-) A-I      intronic chr16:74670760(-) A-I      intronic chr16:74663327(-) A-I      intronic chr16:74697077(-) A-I      intronic chr16:74685298(-) A-I      intronic chr16:74667736(-) A-I      intronic chr16:74674113(-) A-I      intronic chr16:74675716(-) A-I      intronic chr16:74665791(-) A-I      intronic chr16:74682325(-) A-I      intronic chr16:74671122(-) A-I      intronic chr16:74690605(-) A-I      intronic chr16:74694275(-) A-I      intronic chr16:74698453(-) A-I      intronic chr16:74659015(-) A-I      intronic chr16:74689418(-) A-I      intronic chr16:74658676(-) A-I      intronic chr16:74671333(-) A-I      intronic chr16:74681586(-) A-I      intronic chr16:74698073(-) A-I      intronic chr16:74690645(-) A-I      intronic chr16:74686986(-) A-I      intronic chr16:74674522(-) A-I      intronic chr16:74694345(-) A-I      intronic chr16:74664131(-) A-I      intronic chr16:74682570(-) A-I      intronic chr16:74658431(-) A-I      intronic chr16:74692758(-) A-I      intronic chr16:74659963(-) A-I      intronic chr16:74682540(-) A-I      intronic chr16:74658846(-) A-I      intronic chr16:74671121(-) A-I      intronic chr16:74675655(-) A-I      intronic chr16:74687232(-) A-I      intronic chr16:74698121(-) A-I      intronic chr16:74664194(-) A-I      intronic chr16:74682489(-) A-I      intronic chr16:74690534(-) A-I      intronic chr16:74687071(-) A-I      intronic chr16:74671517(-) A-I      intronic chr16:74666838(-) A-I      intronic chr16:74675566(-) A-I      intronic chr16:74680572(-) A-I      intronic chr16:74675564(-) A-I      intronic chr16:74667591(-) A-I      intronic chr16:74699429(-) A-I      intronic chr16:74696979(-) A-I      intronic chr16:74681324(-) A-I      intronic chr16:74674222(-) A-I      intronic chr16:74692810(-) A-I      intronic chr16:74696980(-) A-I      intronic chr16:74675153(-) A-I      intronic chr16:74667053(-) A-I      intronic chr16:74674159(-) A-I      intronic chr16:74682503(-) A-I      intronic chr16:74685393(-) A-I      intronic chr16:74681224(-) A-I      intronic chr16:74674140(-) A-I      intronic chr16:74685417(-) A-I      intronic chr16:74677152(-) A-I      intronic chr16:74680081(-) A-I      intronic chr16:74680100(-) A-I      intronic chr16:74699228(-) A-I      intronic chr16:74658422(-) A-I      intronic chr16:74667796(-) A-I      intronic chr16:74687365(-) A-I      intronic chr16:74674108(-) A-I      intronic chr16:74682414(-) A-I      intronic chr16:74685395(-) A-I      intronic chr16:74681917(-) A-I      intronic chr16:74675680(-) A-I      intronic chr16:74685465(-) A-I      intronic chr16:74690072(-) A-I      intronic chr16:74666792(-) A-I      intronic chr16:74665764(-) A-I      intronic chr16:74675786(-) A-I      intronic chr16:74680435(-) A-I      intronic chr16:74663300(-) A-I      intronic chr16:74675741(-) A-I      intronic chr16:74663487(-) A-I      intronic chr16:74694268(-) A-I      intronic chr16:74686851(-) A-I      intronic chr16:74674223(-) A-I      intronic chr16:74676301(-) A-I      intronic chr16:74658659(-) A-I      intronic chr16:74681380(-) A-I      intronic chr16:74690084(-) A-I      intronic chr16:74674103(-) A-I      intronic chr16:74676544(-) A-I      intronic chr16:74666032(-) A-I      intronic chr16:74693487(-) A-I      intronic chr16:74658683(-) A-I      intronic chr16:74674217(-) A-I      intronic chr16:74699869(-) A-I      intronic chr16:74674276(-) A-I      intronic chr16:74681991(-) A-I      intronic chr16:74663481(-) A-I      intronic chr16:74674473(-) A-I      intronic chr16:74693393(-) A-I      intronic chr16:74697074(-) A-I      intronic chr16:74681459(-) A-I      intronic chr16:74666769(-) A-I      intronic chr16:74675702(-) A-I      intronic chr16:74685434(-) A-I      intronic chr16:74681326(-) A-I      intronic chr16:74674581(-) A-I      intronic chr16:74665790(-) A-I      intronic chr16:74658440(-) A-I      intronic chr16:74681587(-) A-I      intronic chr16:74680090(-) A-I      intronic chr16:74689696(-) A-I      intronic chr16:74675579(-) A-I      intronic chr16:74698057(-) A-I      intronic chr16:74663445(-) A-I      intronic chr16:74658527(-) A-I      intronic chr16:74681453(-) A-I      intronic chr16:74699828(-) A-I      intronic chr16:74676215(-) A-I      intronic chr16:74680554(-) A-I      intronic chr16:74694229(-) A-I      intronic chr16:74674182(-) A-I      intronic chr16:74685435(-) A-I      intronic chr16:74681627(-) A-I      intronic chr16:74675157(-) A-I      intronic chr16:74679851(-) A-I      intronic chr16:74686992(-) A-I      intronic chr16:74675712(-) A-I      intronic chr16:74679523(-) A-I      intronic chr16:74699877(-) A-I      intronic chr16:74698488(-) A-I      intronic chr16:74686846(-) A-I      intronic chr16:74664158(-) A-I      intronic chr16:74680101(-) A-I      intronic chr16:74693410(-) A-I      intronic chr16:74687507(-) A-I      intronic chr16:74664269(-) A-I      intronic chr16:74676188(-) A-I      intronic chr16:74690048(-) A-I      intronic chr16:74667748(-) A-I      intronic chr16:74658367(-) A-I      intronic chr16:74671406(-) A-I      intronic chr16:74666789(-) A-I      intronic chr16:74697075(-) A-I      intronic chr16:74679789(-) A-I      intronic chr16:74667726(-) A-I      intronic chr16:74693947(-) A-I      intronic chr16:74690073(-) A-I      intronic chr16:74668431(-) A-I      intronic chr16:74665335(-) A-I      intronic chr16:74664268(-) A-I      intronic chr16:74675240(-) A-I      intronic chr16:74681678(-) A-I      intronic chr16:74681601(-) A-I      intronic chr16:74682366(-) A-I      intronic chr16:74679918(-) A-I      intronic chr16:74682732(-) A-I      intronic chr16:74689417(-) A-I      intronic chr16:74687057(-) A-I      intronic chr16:74675333(-) A-I      intronic chr16:74674715(-) A-I      intronic chr16:74680781(-) A-I      intronic chr16:74676153(-) A-I      intronic chr16:74666837(-) A-I      intronic chr16:74680222(-) A-I      intronic chr16:74690602(-) A-I      intronic chr16:74689697(-) A-I      intronic chr16:74692782(-) A-I      intronic chr16:74665723(-) A-I      intronic chr16:74671124(-) A-I      intronic chr16:74671690(-) A-I      intronic chr16:74666918(-) A-I      intronic chr16:74674117(-) A-I      intronic chr16:74655721(-) A-I      3'UTR chr16:74656352(-) A-I      3'UTR chr16:74656305(-) A-I      3'UTR chr16:74655677(-) A-I      3'UTR chr16:74655731(-) A-I      3'UTR chr16:74655704(-) A-I      3'UTR

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	RFWD3	chr16:74698523(-) A-I intron -    15258596 chr16:74698525(-) A-I intron -    15258596   //21960545 chr16:74698531(-) A-I intron -    15258596 chr16:74664194(-) A-I intron -    22327324 chr16:74698535(-) A-I intron -    21960545 chr16:74655592(-) A-C exon 3'UTR    22484847 chr16:74655597(-) C-A exon 3'UTR    22484847 chr16:74655598(-) A-C exon 3'UTR    22484847 chr16:74656361(-) T-G exon 3'UTR    22484847 chr16:74656367(-) T-A exon 3'UTR    22484847 chr16:74663377(-) A-G intron -    22484847 chr16:74663481(-) A-G intron -    22484847 chr16:74663487(-) A-G intron -    22484847 chr16:74664193(-) A-G intron -    22484847 chr16:74664194(-) A-G intron -    22484847 chr16:74665790(-) A-G intron -    22484847 chr16:74674103(-) A-G intron -    22484847 chr16:74675702(-) A-G intron -    22484847 chr16:74675716(-) A-G intron -    22484847 chr16:74675748(-) A-G intron -    22484847 chr16:74677151(-) A-G intron -    22484847 chr16:74682949(-) A-G intron -    22484847 chr16:74693410(-) A-G intron -    22484847 chr16:74698135(-) A-G intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	RFWD3	chr16:74655385-74655386(-) m6A 3'UTR       27773535 chr16:74655432-74655433(-) m6A 3'UTR       27773535 chr16:74655551-74655552(-) m6A 3'UTR       24981863//27773535 chr16:74655583-74655584(-) m6A 3'UTR       24981863//27773535 chr16:74655593-74655594(-) m6A 3'UTR       24981863//27773535 chr16:74655601-74655602(-) m6A 3'UTR       24981863//27773535 chr16:74655618-74655619(-) m6A 3'UTR       24981863//27773535 chr16:74656317-74656318(-) m6A 3'UTR       - chr16:74657370-74657371(-) Tm 3'UTR       - chr16:74657666-74657667(-) m6A 3'UTR       - chr16:74657682-74657683(-) m6A 3'UTR       - chr16:74657724-74657725(-) m6A 3'UTR       24284625//24981863 chr16:74657811-74657812(-) m6A 3'UTR       24284625//24981863//27773535 chr16:74657886-74657887(-) m6A CDS       24284625//24981863//27371828//22575960 chr16:74657907-74657908(-) m6A CDS       24284625//24981863//27371828//22575960 chr16:74657926-74657927(-) m6A CDS       24284625//24981863//27371828//22575960 chr16:74657934-74657935(-) m6A CDS       24284625//24981863//27371828//22575960 chr16:74660330-74660331(-) m6A CDS       24284625//27773535 chr16:74660342-74660343(-) m6A CDS       24284625//27773535 chr16:74662384-74662385(-) m6A CDS       24284625//27773535 chr16:74666503-74666504(-) m6A CDS//exon       27773535 chr16:74670348-74670349(-) m6A CDS       27773535 chr16:74670463-74670464(-) m6A CDS       27773535 chr16:74671837-74671838(-) m6A CDS//exon       27773535 chr16:74671852-74671853(-) m6A CDS//exon       27773535 chr16:74678276-74678277(-) m6A CDS//exon       - chr16:74678282-74678283(-) Tm CDS//exon       - chr16:74678286-74678287(-) m6A CDS//exon       - chr16:74678302-74678303(-) m6A CDS//exon       - chr16:74683032-74683033(-) m6A 5'UTR//CDS//exon       27773535 chr16:74683043-74683044(-) m6A 5'UTR//CDS//exon       27773535 chr16:74685940-74685941(-) m6A 5'UTR//CDS       24209618//27773535//27371828 chr16:74685979-74685980(-) m6A 5'UTR//CDS       24284625//24209618//27773535//27371828 chr16:74694627-74694628(-) m6A exon//intron       25456834//27773535 chr16:74694644-74694645(-) m6A exon//intron       27773535 chr16:74694753-74694754(-) m6A exon//intron       24981863//27773535//27371828//22608085 chr16:74694775-74694776(-) m6A exon//intron       24284625//25456834//24981863//27773535//27371828//22608085 chr16:74694842-74694843(-) m6A CDS//exon//intron       24284625//24209618//25456834//24981863//27773535//27371828//22575960//22608085 chr16:74694896-74694897(-) m6A CDS//exon//intron       24284625//24209618//25456834//24981863//27773535//27371828//27773536//22575960//22608085 chr16:74694919-74694920(-) m6A CDS//exon//intron       24284625//24209618//25456834//24981863//26404942//27773535//27371828//27773536//22575960//22608085 chr16:74694933-74694934(-) m6A CDS//exon//intron       24284625//24209618//25456834//24981863//26404942//27773535//27371828//27773536//22575960//22608085 chr16:74694960-74694961(-) m6A CDS//exon//intron       24284625//24209618//25456834//24981863//26404942//27773535//27371828//27773536//22575960//22608085 chr16:74694969-74694970(-) m6A CDS//exon//intron       24284625//24209618//25456834//24981863//26404942//27773535//27371828//27773536//22575960//22608085 chr16:74695047-74695048(-) m6A CDS//exon//intron       24284625//24209618//25456834//24981863//27773535//27371828//27773536//22575960//22608085 chr16:74695055-74695056(-) m6A CDS//exon//intron       24284625//24209618//25456834//24981863//27773535//27371828//27773536//22575960//22608085 chr16:74695069-74695070(-) m6A CDS//exon//intron       24284625//24209618//25456834//24981863//27773535//27371828//27773536//22575960//22608085 chr16:74695081-74695082(-) m6A CDS//exon//intron       24284625//24209618//25456834//24981863//27773535//27371828//27773536//22575960//22608085 chr16:74695251-74695252(-) m6A CDS//exon//intron       24284625//27773535 chr16:74695293-74695294(-) m6A CDS//exon//intron       24284625//27773535 chr16:74698527-74698528(-) m6A 5'UTR//exon//intron       24209618 chr16:74699139-74699140(-) m6A 5'UTR//intron       24209618

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	kshv-miR-K12-8-3p	Exosome Jurkat-derived T cell line (J77cl20)     21505438
	RFWD3	Chromatin K562 cells     - Cytosol HCC cell line (HepG2)|K562 cells     - Exosome Blood     - Insoluble cytoplasm K562 cells     - Membrane HCC cell line (HepG2)|K562 cells     - Nucleolus K562 cells     - Nucleoplasm K562 cells     - Nucleus HCC cell line (HepG2)|HeLa-S3 cells|K562 cells     -

Interaction Network (The top 100 interactions):

Interactor1: kshv-miR-K12-8-3p
Interactor2: RFWD3

Evidence Support:

Weak-Evidence	Microarray
Prediction-Evidence	cRep//miRanda//Targetscan
Support Database	RepTar//ViRBase//VmiReg

References:

PMID	22174674	Target region	None
Source	RepTar//ViRBase//VmiReg	Interactor1 expression	None
Tissue or cell line	EA.hy926 cells	Interactor2 expression	Downregulation
Description	As KSHV putative direct targets we extracted transcripts that were significantly down-regulated significantly in the replicate experiments, and which contained at least one seed-match to one of the KSHV miRNAs. We identified 704 putative direct targets in DG-75 cells (Figure 3B), and 980 putative direct targets in EA.hy926 cells (Figure 3C). A complete list of putative direct targets can be found in Dataset S2 for DG-75 cells and in Dataset S3 for EA.hy926 cells. The overlap between the two datasets contained 153 putative direct targets (Dataset S4).