Basic Information:

  VIRBase ID:  

VHID00446741

  Virus:  

Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)

  Host:  

Homo sapiens

  Confidence Score:  

0.6002

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      kshv-miR-K12-8-3p:  MIMAT0002186          ZNF586:  NM_017652     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	kshv-miR-K12-8-3p	ZNF586
miRBase Accession/Entrez ID	MIMAT0002186	54807
Organism	Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	-

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	ZNF586	chr19:58290128(+) A-I      NonSyn:His->Arg chr19:58301758(+) A-I      NonSyn:Ile->Met chr19:58301756(+) A-I      NonSyn:Ile->Val chr19:58301703(+) A-I      NonSyn:Asn->Ser chr19:58301702(+) A-I      NonSyn:Asn->Asp chr19:58287993(+) A-I      NonSyn:Tyr->Cys chr19:58285413(+) A-I      intronic chr19:58290085(+) A-I      intronic chr19:58290097(+) A-I      intronic chr19:58290080(+) A-I      intronic chr19:58285406(+) A-I      intronic chr19:58285400(+) A-I      intronic chr19:58289557(+) A-I      intronic chr19:58289543(+) A-I      intronic chr19:58289540(+) A-I      intronic chr19:58289539(+) A-I      intronic chr19:58289514(+) A-I      intronic chr19:58283398(+) A-I      intronic chr19:58283395(+) A-I      intronic chr19:58283293(+) A-I      intronic chr19:58283292(+) A-I      intronic chr19:58283287(+) A-I      intronic chr19:58285486(+) A-I      intronic chr19:58289508(+) A-I      intronic chr19:58287072(+) A-I      intronic chr19:58289581(+) A-I      intronic chr19:58287070(+) A-I      intronic chr19:58287019(+) A-I      intronic chr19:58285629(+) A-I      intronic chr19:58286387(+) A-I      intronic chr19:58285424(+) A-I      intronic chr19:58286531(+) A-I      intronic chr19:58286833(+) A-I      intronic chr19:58282286(+) A-I      intronic chr19:58289579(+) A-I      intronic chr19:58282296(+) A-I      intronic chr19:58283256(+) A-I      intronic chr19:58286421(+) A-I      intronic chr19:58286530(+) A-I      intronic chr19:58319051(+) A-I      intronic chr19:58287051(+) A-I      intronic chr19:58282262(+) A-I      intronic chr19:58281767(+) A-I      intronic chr19:58283251(+) A-I      intronic chr19:58287071(+) A-I      intronic chr19:58284852(+) A-I      intronic chr19:58281664(+) A-I      intronic chr19:58286792(+) A-I      intronic chr19:58281755(+) A-I      intronic chr19:58286756(+) A-I      intronic chr19:58288558(+) A-I      intronic chr19:58283322(+) A-I      intronic chr19:58282467(+) A-I      intronic chr19:58285312(+) A-I      intronic chr19:58283358(+) A-I      intronic chr19:58285322(+) A-I      intronic chr19:58285350(+) A-I      intronic chr19:58318844(+) A-I      intronic chr19:58285388(+) A-I      intronic chr19:58289307(+) A-I      intronic chr19:58286504(+) A-I      intronic chr19:58285705(+) A-I      intronic chr19:58286570(+) A-I      intronic chr19:58289257(+) A-I      intronic chr19:58285605(+) A-I      intronic chr19:58285704(+) A-I      intronic chr19:58283464(+) A-I      intronic chr19:58287100(+) A-I      intronic chr19:58286752(+) A-I      intronic chr19:58318878(+) A-I      intronic chr19:58282788(+) A-I      intronic chr19:58286436(+) A-I      intronic chr19:58285940(+) A-I      intronic chr19:58285771(+) A-I      intronic chr19:58281693(+) A-I      intronic chr19:58285403(+) A-I      intronic chr19:58286759(+) A-I      intronic chr19:58285734(+) A-I      intronic chr19:58281654(+) A-I      intronic chr19:58286845(+) A-I      intronic chr19:58281754(+) A-I      intronic chr19:58285465(+) A-I      intronic chr19:58288575(+) A-I      intronic chr19:58287035(+) A-I      intronic chr19:58285359(+) A-I      intronic chr19:58286423(+) A-I      intronic chr19:58282319(+) A-I      intronic chr19:58284872(+) A-I      intronic chr19:58282256(+) A-I      intronic chr19:58285726(+) A-I      intronic chr19:58285493(+) A-I      intronic chr19:58289618(+) A-I      intronic

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	ZNF586	chr19:58282262(+) A-I intron -    21960545 chr19:58286421(+) A-I intron -    21960545 chr19:58286423(+) A-I intron -    21960545 chr19:58281755(+) A-G intron -    22484847 chr19:58285322(+) A-G intron -    22484847 chr19:58285388(+) A-G intron -    22484847 chr19:58286570(+) A-G intron -    22484847 chr19:58288558(+) A-G intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	ZNF586	chr19:58281156-58281157(+) m6A 5'UTR       24981863 chr19:58281161-58281162(+) m6A 5'UTR       24981863 chr19:58281173-58281174(+) m6A 5'UTR       24981863 chr19:58290187-58290188(+) m6A CDS//intron       24981863 chr19:58290250-58290251(+) m6A CDS//intron       24284625//24981863//27773535//22608085 chr19:58290269-58290270(+) m6A CDS//intron       24284625//24981863//27773535//22608085 chr19:58290287-58290288(+) m6A CDS//intron       24284625//24981863//27773535//22608085 chr19:58290399-58290400(+) m6A CDS//intron       24284625//24981863//22608085 chr19:58290462-58290463(+) m6A CDS//intron       24284625//24981863//22608085 chr19:58291024-58291025(+) m6A 3'UTR//CDS//intron       24284625//24981863//22608085 chr19:58291040-58291041(+) m6A 3'UTR//CDS//intron       24284625//24981863//22608085 chr19:58291448-58291449(+) m6A 3'UTR//intron       24284625 chr19:58291524-58291525(+) m6A 3'UTR//intron       24284625//24981863//22608085 chr19:58291564-58291565(+) m6A 3'UTR//intron       24284625//24981863//22608085 chr19:58291760-58291761(+) m6A 3'UTR//intron       24284625//24209618//24981863//22608085 chr19:58291785-58291786(+) m6A 3'UTR//intron       24284625//24209618//24981863//22608085 chr19:58291811-58291812(+) m6A 3'UTR//intron       24284625//24981863//22608085 chr19:58291890-58291891(+) m6A 3'UTR//intron       24284625//24981863//22608085 chr19:58291946-58291947(+) m6A 3'UTR//intron       24284625 chr19:58301672-58301673(+) m6A CDS//intron       24284625//27773535 chr19:58331151-58331152(+) m6A 3'UTR       - chr19:58331164-58331165(+) m6A 3'UTR       - chr19:58331178-58331179(+) m6A 3'UTR       - chr19:58331197-58331198(+) m6A 3'UTR       -

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	kshv-miR-K12-8-3p	Exosome Jurkat-derived T cell line (J77cl20)     21505438
	ZNF586	Chromatin K562 cells     - Exosome Blood     - Insoluble cytoplasm HCC cell line (HepG2)     - Nucleolus K562 cells     - Nucleoplasm K562 cells     -

Interaction Network (The top 100 interactions):

Interactor1: kshv-miR-K12-8-3p
Interactor2: ZNF586

Evidence Support:

Weak-Evidence	PAR-CLIP
Prediction-Evidence	cRep//PARalyzer//Targetscan
Support Database	RepTar//ViRBase//VIRmiRNA//VmiReg

References:

PMID	22100165	Target region	3'UTR
Source	RepTar//ViRBase//VIRmiRNA//VmiReg	Interactor1 expression	None
Tissue or cell line	BC-1 cells//BC-3 cells//BJAB cells	Interactor2 expression	None
Description	Using PAR-CLIP, a technology which allows the direct and transcriptome-wide identification of miRNA targets, we delineate the target sites for all viral and cellular miRNAs expressed in PEL cell lines. The resulting data set revealed that KSHV miRNAs directly target more than 2000 cellular mRNAs, including many involved in pathways relevant to KSHV pathogenesis. Moreover, 58% of these mRNAs are also targeted by EBV miRNAs, via distinct binding sites.Clusters with seed matches to expressed miRNAs were considered candidate target sites. Of the clusters mapping to human 3'UTRs, 69% (BC-1) and 70% (BC-3) had seed matches to expressed miRNAs (Tables S6).Table S6A BC-1 derived 3'UTR clusters with seed matches to expressed miRNAs.