Basic Information:

  VIRBase ID:  

VHID00446673

  Virus:  

Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)

  Host:  

Homo sapiens

  Confidence Score:  

0.6002

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      kshv-miR-K12-8-3p:  MIMAT0002186          PNKD:  NM_022572     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	kshv-miR-K12-8-3p	PNKD
miRBase Accession/Entrez ID	MIMAT0002186	25953
Organism	Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	BRP17//DYT8//FKSG19//FPD1//KIPP1184//MR-1//MR-1S//MR1//PDC//PKND1//PNKD1//R1//TAHCCP2

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	PNKD	chr2:219174639(+) A-I      intronic chr2:219169009(+) A-I      intronic chr2:219168996(+) A-I      intronic chr2:219168970(+) A-I      intronic chr2:219166999(+) A-I      intronic chr2:219166996(+) A-I      intronic chr2:219198560(+) A-I      intronic chr2:219178043(+) A-I      intronic chr2:219193702(+) A-I      intronic chr2:219195956(+) A-I      intronic chr2:219198580(+) A-I      intronic chr2:219202035(+) A-I      intronic chr2:219202652(+) A-I      intronic chr2:219202661(+) A-I      intronic chr2:219203228(+) A-I      intronic chr2:219203555(+) A-I      intronic chr2:219203615(+) A-I      intronic chr2:219203619(+) A-I      intronic chr2:219193681(+) A-I      intronic chr2:219193676(+) A-I      intronic chr2:219193675(+) A-I      intronic chr2:219189538(+) A-I      intronic chr2:219185232(+) A-I      intronic chr2:219185212(+) A-I      intronic chr2:219178820(+) A-I      intronic chr2:219185273(+) A-I      intronic chr2:219189547(+) A-I      intronic chr2:219177388(+) A-I      intronic chr2:219177973(+) A-I      intronic chr2:219170248(+) A-I      intronic chr2:219189579(+) A-I      intronic chr2:219190057(+) A-I      intronic chr2:219177389(+) A-I      intronic chr2:219169784(+) A-I      intronic chr2:219162614(+) A-I      intronic chr2:219166239(+) A-I      intronic chr2:219177546(+) A-I      intronic chr2:219179546(+) A-I      intronic chr2:219174237(+) A-I      intronic chr2:219173005(+) A-I      intronic chr2:219161675(+) A-I      intronic chr2:219158120(+) A-I      intronic chr2:219166348(+) A-I      intronic chr2:219189602(+) A-I      intronic chr2:219172990(+) A-I      intronic chr2:219201316(+) A-I      intronic chr2:219189947(+) A-I      intronic chr2:219179648(+) A-I      intronic chr2:219169783(+) A-I      intronic chr2:219179665(+) A-I      intronic chr2:219159304(+) A-I      intronic chr2:219178008(+) A-I      intronic chr2:219173704(+) A-I      intronic chr2:219174188(+) A-I      intronic chr2:219179693(+) A-I      intronic chr2:219174400(+) A-I      intronic chr2:219190203(+) A-I      intronic chr2:219179991(+) A-I      intronic chr2:219177362(+) A-I      intronic chr2:219177449(+) A-I      intronic chr2:219166248(+) A-I      intronic chr2:219190191(+) A-I      intronic chr2:219166255(+) A-I      intronic

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	PNKD	chr2:219189537(+) A-G intron -    22484847 chr2:219189547(+) A-G intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	PNKD	chr2:219135209-219135210(+) m6A 5'UTR       24981863 chr2:219135256-219135257(+) m6A 5'UTR//exon       24981863 chr2:219135337-219135338(+) m6A exon//intron       24284625//24981863 chr2:219135354-219135355(+) m6A exon//intron       24284625 chr2:219135732-219135733(+) m6A exon//intron       - chr2:219135777-219135778(+) m6A exon//intron       - chr2:219135853-219135854(+) m6A exon//intron       - chr2:219136147-219136148(+) m6A CDS//exon       24981863 chr2:219136191-219136192(+) m6A CDS//exon       24981863 chr2:219136197-219136198(+) m6A CDS//exon       - chr2:219136244-219136245(+) m6A CDS//exon       - chr2:219136263-219136264(+) m6A CDS//exon       - chr2:219137348-219137349(+) m6A CDS//exon//intron       24284625 chr2:219137354-219137355(+) m6A CDS//exon//intron       24284625 chr2:219137384-219137385(+) m6A CDS//exon//intron       24284625 chr2:219137479-219137480(+) m6A CDS//exon//intron       24284625//24981863//22575960 chr2:219137599-219137600(+) m6A 3'UTR//exon//intron       24284625//24981863//22575960//22608085 chr2:219182841-219182842(+) m6A exon//intron       25456834//22608085 chr2:219182874-219182875(+) m6A exon//intron       25456834//22608085 chr2:219182883-219182884(+) m6A exon//intron       25456834//22608085 chr2:219182909-219182910(+) m6A exon//intron       25456834//22608085 chr2:219182920-219182921(+) m6A exon//intron       25456834//22608085 chr2:219182928-219182929(+) m6A exon//intron       25456834//22608085 chr2:219182948-219182949(+) m6A exon//intron       25456834//22608085 chr2:219182966-219182967(+) m6A exon//intron       25456834//22608085 chr2:219206843-219206844(+) m6A CDS       24284625 chr2:219208227-219208228(+) m6A CDS       24284625 chr2:219208248-219208249(+) m6A CDS       24284625 chr2:219208267-219208268(+) m6A CDS       24284625 chr2:219209198-219209199(+) m6A CDS       24284625 chr2:219209231-219209232(+) m6A CDS       24284625 chr2:219209578-219209579(+) m6A CDS       24284625//24209618 chr2:219209865-219209866(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//22608085 chr2:219209905-219209906(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//22575960//22608085 chr2:219209960-219209961(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//22575960//22608085 chr2:219209970-219209971(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//22575960//22608085 chr2:219210027-219210028(+) m6A 3'UTR       24284625//24209618//25456834//24981863//22575960//22608085 chr2:219210085-219210086(+) m6A 3'UTR       24284625//24209618//25456834//24981863//22575960 chr2:219210197-219210198(+) m6A 3'UTR       24284625//24209618//25456834//24981863//22575960 chr2:219210213-219210214(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//22575960 chr2:219210272-219210273(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942 chr2:219210290-219210291(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942 chr2:219211116-219211117(+) m6A 3'UTR       24284625 chr2:219211147-219211148(+) m6A 3'UTR       24284625 chr2:219211394-219211395(+) m6A 3'UTR       - chr2:219211417-219211418(+) m6A 3'UTR       -

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	kshv-miR-K12-8-3p	Exosome Jurkat-derived T cell line (J77cl20)     21505438
	PNKD	Chromatin K562 cells     - Endoplasmic reticulum Human myelogenous leukemia cell line (K-562)     21613539 Exosome Blood     - Nucleolus K562 cells     - Nucleoplasm K562 cells     - Nucleus HCC cell line (HepG2)|K562 cells     - Ribosome Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: kshv-miR-K12-8-3p
Interactor2: PNKD

Evidence Support:

Weak-Evidence	Microarray
Prediction-Evidence	cRep//miRanda//Targetscan
Support Database	RepTar//ViRBase//VmiReg

References:

PMID	22174674	Target region	None
Source	RepTar//ViRBase//VmiReg	Interactor1 expression	None
Tissue or cell line	DG-75 cells	Interactor2 expression	Downregulation
Description	As KSHV putative direct targets we extracted transcripts that were significantly down-regulated significantly in the replicate experiments, and which contained at least one seed-match to one of the KSHV miRNAs. We identified 704 putative direct targets in DG-75 cells (Figure 3B), and 980 putative direct targets in EA.hy926 cells (Figure 3C). A complete list of putative direct targets can be found in Dataset S2 for DG-75 cells and in Dataset S3 for EA.hy926 cells. The overlap between the two datasets contained 153 putative direct targets (Dataset S4).