Basic Information:

  VIRBase ID:  

VHID00446476

  Virus:  

Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)

  Host:  

Homo sapiens

  Confidence Score:  

0.6040

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      kshv-miR-K12-8-3p:  MIMAT0002186          TGFBR2:  NM_001024847     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	kshv-miR-K12-8-3p	TGFBR2
miRBase Accession/Entrez ID	MIMAT0002186	7048
Organism	Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	AAT3//FAA3//LDS1B//LDS2//LDS2B//MFS2//RIIC//TAAD2//TBR-ii//TBRII//TGFbeta-RII//TGFR-2

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	TGFBR2	chr3:30664265(+) A-I      intronic chr3:30664284(+) A-I      intronic chr3:30664287(+) A-I      intronic chr3:30664293(+) A-I      intronic chr3:30664304(+) A-I      intronic chr3:30665746(+) A-I      intronic chr3:30665745(+) A-I      intronic chr3:30665726(+) A-I      intronic chr3:30665720(+) A-I      intronic chr3:30665709(+) A-I      intronic chr3:30665707(+) A-I      intronic chr3:30665703(+) A-I      intronic chr3:30720027(+) A-I      intronic chr3:30719947(+) A-I      intronic chr3:30664329(+) A-I      intronic chr3:30664313(+) A-I      intronic chr3:30664297(+) A-I      intronic chr3:30664269(+) A-I      intronic chr3:30664241(+) A-I      intronic chr3:30664229(+) A-I      intronic chr3:30664225(+) A-I      intronic chr3:30664124(+) A-I      intronic chr3:30664119(+) A-I      intronic chr3:30664118(+) A-I      intronic chr3:30661424(+) A-I      intronic chr3:30669104(+) A-I      intronic chr3:30669107(+) A-I      intronic chr3:30669135(+) A-I      intronic chr3:30669078(+) A-I      intronic chr3:30664110(+) A-I      intronic chr3:30735159(+) A-I      3'UTR chr3:30665855(+) A-I      intronic chr3:30710514(+) A-I      intronic chr3:30717074(+) A-I      intronic chr3:30719919(+) A-I      intronic chr3:30716991(+) A-I      intronic chr3:30664103(+) A-I      intronic chr3:30716983(+) A-I      intronic chr3:30665854(+) A-I      intronic chr3:30710654(+) A-I      intronic chr3:30710520(+) A-I      intronic chr3:30710661(+) A-I      intronic chr3:30719893(+) A-I      intronic chr3:30719961(+) A-I      intronic

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	TGFBR2	chr3:30648140-30648141(+) m6A 5'UTR       24981863 chr3:30648151-30648152(+) m6A 5'UTR       24209618//24981863 chr3:30664743-30664744(+) m6A CDS//intron       26404942 chr3:30664760-30664761(+) m6A CDS//intron       26404942 chr3:30691778-30691779(+) m6A CDS       24284625//26404942 chr3:30691792-30691793(+) m6A CDS       26404942 chr3:30691891-30691892(+) m6A CDS       26404942 chr3:30713281-30713282(+) m6A CDS       26404942 chr3:30713414-30713415(+) m6A CDS       24284625//24981863//26404942//27773535//22608085 chr3:30713471-30713472(+) m6A CDS       24284625//25456834//24981863//26404942//27773535//22575960//22608085 chr3:30713491-30713492(+) m6A CDS       24284625//25456834//24981863//26404942//27773535//22575960//22608085 chr3:30713539-30713540(+) m6A CDS       24284625//25456834//24981863//26404942//27773535//22575960//22608085 chr3:30713549-30713550(+) m6A CDS       24284625//25456834//24981863//26404942//27773535//22575960//22608085 chr3:30713561-30713562(+) m6A CDS       24284625//25456834//24981863//26404942//27773535//22575960//22608085 chr3:30713582-30713583(+) m6A CDS       24284625//25456834//24981863//26404942//27773535//22575960//22608085 chr3:30713631-30713632(+) m6A CDS       24284625//25456834//24981863//26404942//22575960//22608085 chr3:30713711-30713712(+) m6A CDS       24284625//24981863//26404942//22575960//22608085 chr3:30713810-30713811(+) m6A CDS       24284625//24981863//26404942//22575960//22608085 chr3:30713888-30713889(+) m6A CDS       24284625//24981863//26404942 chr3:30715602-30715603(+) m6A CDS       24981863 chr3:30715674-30715675(+) m6A CDS       - chr3:30729944-30729945(+) m6A CDS       - chr3:30732951-30732952(+) m6A CDS       24284625//24981863//26404942 chr3:30733020-30733021(+) m6A CDS       24284625//24981863//26404942 chr3:30733077-30733078(+) m6A CDS       24284625//24981863//26404942 chr3:30733145-30733146(+) m6A 3'UTR       25456834//24981863//26404942//22608085 chr3:30733172-30733173(+) m6A 3'UTR       24284625//25456834//24981863//26404942//22608085 chr3:30733190-30733191(+) m6A 3'UTR       24284625//25456834//24981863//26404942 chr3:30733237-30733238(+) m6A 3'UTR       24284625//25456834//24981863//26404942 chr3:30733467-30733468(+) m6A 3'UTR       26404942 chr3:30733477-30733478(+) m6A 3'UTR       26404942 chr3:30733514-30733515(+) m6A 3'UTR       26404942 chr3:30733522-30733523(+) m6A 3'UTR       26404942 chr3:30733528-30733529(+) Cm 3'UTR       - chr3:30733542-30733543(+) m6A 3'UTR       26404942 chr3:30733599-30733600(+) m6A 3'UTR       26404942 chr3:30733606-30733607(+) m6A 3'UTR       26404942 chr3:30733783-30733784(+) m6A 3'UTR       - chr3:30733831-30733832(+) m6A 3'UTR       27773535 chr3:30733855-30733856(+) m6A 3'UTR       27773535 chr3:30733890-30733891(+) m6A 3'UTR       27773535 chr3:30733929-30733930(+) m6A 3'UTR       27773535 chr3:30734129-30734130(+) m6A 3'UTR       26404942 chr3:30734225-30734226(+) m6A 3'UTR       26404942 chr3:30734247-30734248(+) m6A 3'UTR       26404942 chr3:30734402-30734403(+) m6A 3'UTR       26404942 chr3:30734435-30734436(+) m6A 3'UTR       26404942 chr3:30734463-30734464(+) m6A 3'UTR       26404942 chr3:30735441-30735442(+) m6A 3'UTR       27773535

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	kshv-miR-K12-8-3p	Exosome Jurkat-derived T cell line (J77cl20)     21505438
	TGFBR2	Cytosol HCC cell line (HepG2)|HeLa-S3 cells     - Exosome Blood     - Membrane HCC cell line (HepG2)     - Nucleus HCC cell line (HepG2)     - Ribosome-free cytosol Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: kshv-miR-K12-8-3p
Interactor2: TGFBR2

Evidence Support:

Weak-Evidence	Microarray
Prediction-Evidence	cRep//miRanda//Rep//Targetscan
Support Database	RepTar//ViRBase//VmiReg

References:

PMID	22174674	Target region	None
Source	RepTar//ViRBase//VmiReg	Interactor1 expression	None
Tissue or cell line	EA.hy926 cells	Interactor2 expression	Downregulation
Description	As KSHV putative direct targets we extracted transcripts that were significantly down-regulated significantly in the replicate experiments, and which contained at least one seed-match to one of the KSHV miRNAs. We identified 704 putative direct targets in DG-75 cells (Figure 3B), and 980 putative direct targets in EA.hy926 cells (Figure 3C). A complete list of putative direct targets can be found in Dataset S2 for DG-75 cells and in Dataset S3 for EA.hy926 cells. The overlap between the two datasets contained 153 putative direct targets (Dataset S4).