Basic Information:

  VIRBase ID:  

VHID00446329

  Virus:  

Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)

  Host:  

Homo sapiens

  Confidence Score:  

0.4752

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      kshv-miR-K12-8-3p:  MIMAT0002186          BCKDHB:  NR_134945     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	kshv-miR-K12-8-3p	BCKDHB
miRBase Accession/Entrez ID	MIMAT0002186	594
Organism	Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	BCKDE1B//BCKDH E1-beta//E1B

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	BCKDHB	chr6:81028237(+) A-I      intronic chr6:81028242(+) A-I      intronic chr6:81052191(+) A-I      intronic chr6:81036515(+) A-I      intronic chr6:81036518(+) A-I      intronic chr6:81018839(+) A-I      intronic chr6:81052426(+) A-I      intronic chr6:80903316(+) A-I      intronic chr6:80903341(+) A-I      intronic chr6:81018386(+) A-I      intronic chr6:81036540(+) A-I      intronic chr6:81019029(+) A-I      intronic chr6:81025481(+) A-I      intronic chr6:81028194(+) A-I      intronic chr6:81018441(+) A-I      intronic chr6:81052299(+) A-I      intronic chr6:81018405(+) A-I      intronic chr6:80829247(+) A-I      intronic chr6:81018933(+) A-I      intronic chr6:80829243(+) A-I      intronic chr6:80829273(+) A-I      intronic chr6:80902677(+) A-I      intronic chr6:80902735(+) A-I      intronic chr6:81052466(+) A-I      intronic chr6:81018880(+) A-I      intronic chr6:81036508(+) A-I      intronic chr6:80941561(+) A-I      intronic chr6:80825471(+) A-I      intronic chr6:80825575(+) A-I      intronic chr6:80825535(+) A-I      intronic chr6:80988996(+) A-I      intronic chr6:81028275(+) A-I      intronic chr6:81028295(+) A-I      intronic chr6:81036327(+) A-I      intronic chr6:81036376(+) A-I      intronic chr6:81036454(+) A-I      intronic chr6:81036458(+) A-I      intronic chr6:80989037(+) A-I      intronic chr6:80989036(+) A-I      intronic chr6:81052183(+) A-I      intronic chr6:81053797(+) A-I      3'UTR chr6:81053782(+) A-I      3'UTR chr6:81053822(+) A-I      3'UTR chr6:81053942(+) A-I      3'UTR chr6:81053781(+) A-I      3'UTR chr6:81052384(+) A-I      intronic chr6:80872658(+) A-I      intronic chr6:80825560(+) A-I      intronic chr6:80828873(+) A-I      intronic chr6:80934227(+) A-I      intronic chr6:81028255(+) A-I      intronic chr6:81018991(+) A-I      intronic chr6:81018505(+) A-I      intronic chr6:80928993(+) A-I      intronic chr6:81018453(+) A-I      intronic chr6:80847759(+) A-I      intronic chr6:80941455(+) A-I      intronic chr6:81052476(+) A-I      intronic chr6:80902675(+) A-I      intronic chr6:80941520(+) A-I      intronic chr6:80902629(+) A-I      intronic chr6:81053766(+) A-I      3'UTR chr6:81054095(+) A-I      3'UTR chr6:80828861(+) A-I      intronic chr6:80877197(+) A-I      intronic chr6:80941521(+) A-I      intronic chr6:81018898(+) A-I      intronic chr6:81028148(+) A-I      intronic chr6:81028243(+) A-I      intronic chr6:80886969(+) A-I      intronic chr6:81019056(+) A-I      intronic chr6:80941517(+) A-I      intronic chr6:81036380(+) A-I      intronic chr6:80902681(+) A-I      intronic chr6:81018874(+) A-I      intronic chr6:81018881(+) A-I      intronic chr6:81052398(+) A-I      intronic chr6:81018572(+) A-I      intronic chr6:80902740(+) A-I      intronic chr6:81052176(+) A-I      intronic chr6:80903330(+) A-I      intronic chr6:80955663(+) A-I      intronic chr6:80903310(+) A-I      intronic chr6:80934239(+) A-I      intronic chr6:80941522(+) A-I      intronic

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	BCKDHB	chr6:80828861(+) A-G intron -    22484847 chr6:80828873(+) A-G intron -    22484847 chr6:80902629(+) A-G intron -    22484847 chr6:80902636(+) A-G intron -    22484847 chr6:80902675(+) A-G intron -    22484847 chr6:80902681(+) A-G intron -    22484847 chr6:80902702(+) A-G intron -    22484847 chr6:80902740(+) A-G intron -    22484847 chr6:80903330(+) A-G intron -    22484847 chr6:80928993(+) A-G intron -    22484847 chr6:80941455(+) A-G intron -    22484847 chr6:80941517(+) A-G intron -    22484847 chr6:80941520(+) A-G intron -    22484847 chr6:80941521(+) A-G intron -    22484847 chr6:80941522(+) A-G intron -    22484847 chr6:81018453(+) A-G intron -    22484847 chr6:81018874(+) A-G intron -    22484847 chr6:81018881(+) A-G intron -    22484847 chr6:81018898(+) A-G intron -    22484847 chr6:81018991(+) A-G intron -    22484847 chr6:81019056(+) A-G intron -    22484847 chr6:81028148(+) A-G intron -    22484847 chr6:81028175(+) A-G intron -    22484847 chr6:81036431(+) T-C intron -    22484847 chr6:81052176(+) A-G intron -    22484847 chr6:81053719(+) G-T intron -    22484847 chr6:81053731(+) G-C intron -    22484847 chr6:81053733(+) T-G intron -    22484847 chr6:81053766(+) A-G intron -    22484847 chr6:81053773(+) A-G intron -    22484847 chr6:81054066(+) T-G intron -    22484847 chr6:81054095(+) A-G intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	BCKDHB	chr6:80877444-80877445(+) Y CDS       26075521 chr6:81053590-81053591(+) m6A 3'UTR//intron       24981863 chr6:81053623-81053624(+) m6A 3'UTR//intron       24981863

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	kshv-miR-K12-8-3p	Exosome Jurkat-derived T cell line (J77cl20)     21505438
	BCKDHB	Chromatin K562 cells     - Cytoplasm Nasopharyngeal carcinoma cells     20498841 Cytosol HCC cell line (HepG2)     - Exosome Blood     - Membrane HCC cell line (HepG2)|K562 cells     - Nucleolus K562 cells     - Nucleoplasm K562 cells     - Nucleus Nasopharyngeal carcinoma cells     20498841 Nucleus HCC cell line (HepG2)|K562 cells     - Ribosome Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: kshv-miR-K12-8-3p
Interactor2: BCKDHB

Evidence Support:

Weak-Evidence	Microarray
Support Database	ViRBase

References:

PMID	22174674	Target region	None
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	EA.hy926 cells	Interactor2 expression	Downregulation
Description	As KSHV putative direct targets we extracted transcripts that were significantly down-regulated significantly in the replicate experiments, and which contained at least one seed-match to one of the KSHV miRNAs. We identified 704 putative direct targets in DG-75 cells (Figure 3B), and 980 putative direct targets in EA.hy926 cells (Figure 3C). A complete list of putative direct targets can be found in Dataset S2 for DG-75 cells and in Dataset S3 for EA.hy926 cells. The overlap between the two datasets contained 153 putative direct targets (Dataset S4).