Basic Information:

  VIRBase ID:  

VHID00446325

  Virus:  

Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)

  Host:  

Homo sapiens

  Confidence Score:  

0.5908

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      kshv-miR-K12-8-3p:  MIMAT0002186          RHOB:  NM_004040     

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Interactor Information:

	Interactor1	Interactor2
Symbol	kshv-miR-K12-8-3p	RHOB
miRBase Accession/Entrez ID	MIMAT0002186	388
Organism	Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	ARH6//ARHB//MST081//MSTP081//RHOH6

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	RHOB	chr2:20646893-20646894(+) m6A 5'UTR       24284625//24209618//25456834//24981863//22575960//22608085 chr2:20647347-20647348(+) m6A CDS       24284625//24209618//25456834//24981863//26404942//22575960//22608085 chr2:20647401-20647402(+) m6A CDS       24284625//24209618//25456834//24981863//26404942//22575960//22608085 chr2:20647419-20647420(+) m6A CDS       24284625//24209618//25456834//24981863//26404942//22575960//22608085 chr2:20647452-20647453(+) m6A CDS       24284625//24209618//25456834//24981863//26404942//22575960//22608085 chr2:20647485-20647486(+) m6A CDS       24284625//24209618//25456834//24981863//26404942//22575960//22608085 chr2:20647494-20647495(+) m6A CDS       24284625//24209618//25456834//24981863//26404942//22575960//22608085 chr2:20647506-20647507(+) m6A CDS       24284625//24209618//25456834//24981863//26404942//22575960//22608085 chr2:20647584-20647585(+) m6A CDS       24284625//24209618//25456834//24981863//26404942//22575960//22608085 chr2:20647636-20647637(+) m6A CDS       24284625//24209618//25456834//24981863//26404942//22575960//22608085 chr2:20647712-20647713(+) m6A CDS       24284625//24209618//25456834//24981863//26404942//22575960//22608085 chr2:20647866-20647867(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//27773536//22575960//22608085 chr2:20647897-20647898(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//27773536//22575960//22608085 chr2:20647914-20647915(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//27773536//22575960//22608085 chr2:20647967-20647968(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//27773536//22575960//22608085 chr2:20648254-20648255(+) m6A 3'UTR       24284625//24209618//24981863//26404942//27773535//22575960//22608085 chr2:20648396-20648397(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//27773535//22575960//22608085 chr2:20648414-20648415(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//27773535//22575960//22608085 chr2:20648419-20648420(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//27773535//22575960//22608085 chr2:20648449-20648450(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//27773535//22575960//22608085 chr2:20648473-20648474(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//27773535//22575960//22608085 chr2:20648517-20648518(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//27773535//22575960//22608085 chr2:20648582-20648583(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//22575960//22608085 chr2:20648625-20648626(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//22575960//22608085 chr2:20648817-20648818(+) m6A 3'UTR       24981863//26404942//27773535

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	kshv-miR-K12-8-3p	Exosome Jurkat-derived T cell line (J77cl20)     21505438
	RHOB	Cytosol Human myelogenous leukemia cell line (K-562)     21613539 Cytosol HeLa-S3 cells     - Exosome Blood     - Membrane HCC cell line (HepG2)     - Nucleus HeLa-S3 cells     - Ribosome Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: kshv-miR-K12-8-3p
Interactor2: RHOB

Evidence Support:

Weak-Evidence	Microarray
Prediction-Evidence	cRep//miRanda
Support Database	RepTar//ViRBase//VmiReg

References:

PMID	22174674	Target region	None
Source	RepTar//ViRBase//VmiReg	Interactor1 expression	None
Tissue or cell line	EA.hy926 cells	Interactor2 expression	Downregulation
Description	As KSHV putative direct targets we extracted transcripts that were significantly down-regulated significantly in the replicate experiments, and which contained at least one seed-match to one of the KSHV miRNAs. We identified 704 putative direct targets in DG-75 cells (Figure 3B), and 980 putative direct targets in EA.hy926 cells (Figure 3C). A complete list of putative direct targets can be found in Dataset S2 for DG-75 cells and in Dataset S3 for EA.hy926 cells. The overlap between the two datasets contained 153 putative direct targets (Dataset S4).