Detail Information

Basic Information:


  VIRBase ID:  

VHID00444808

  Virus:  

Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)

  Host:  

Homo sapiens

  Confidence Score:  

0.9797

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      kshv-miR-K12-9-3p:           IRAK1:      

      *It may take a few minutes to display results.

Interactor Information:


Interactor1 Interactor2
Symbol kshv-miR-K12-9-3p IRAK1
miRBase
Accession/Entrez ID		 MIMAT0002185 3654
Organism Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV) Homo sapiens
Category miRNA mRNA
Alias - IRAK//pelle

RNA Editing:


Resource Symbol Editing Position Change Genetic Region
RADAR IRAK1
chrX:153279602(-) A-I      NonSyn:His->Arg
chrX:153276588(-) A-I      3'UTR
chrX:153276744(-) A-I      3'UTR
chrX:153276791(-) A-I      3'UTR
chrX:153276814(-) A-I      3'UTR
chrX:153276750(-) A-I      3'UTR
chrX:153276813(-) A-I      3'UTR
chrX:153276668(-) A-I      3'UTR
chrX:153276687(-) A-I      3'UTR
chrX:153276787(-) A-I      3'UTR
chrX:153276634(-) A-I      3'UTR
chrX:153276650(-) A-I      3'UTR
chrX:153280137(-) A-I      intronic
chrX:153279877(-) A-I      intronic
Resource Symbol Editing Position Change SeqReg exReg PMID
DARNED IRAK1
chrX:153276566(-) A-G exon 3'UTR    22484847
chrX:153276668(-) A-G exon 3'UTR    22484847
chrX:153276687(-) A-G exon 3'UTR    22484847
chrX:153276787(-) A-G exon 3'UTR    22484847

RNA Modification:


Resource Symbol ModificationPosition Type Genomic Context PMID
RMBase IRAK1
chrX:153276155-153276156(-) m6A 3'UTR       22575960
chrX:153276172-153276173(-) m6A 3'UTR//exon       22575960
chrX:153276185-153276186(-) m6A 3'UTR//exon       22575960
chrX:153276230-153276231(-) m6A 3'UTR//exon       22575960
chrX:153276244-153276245(-) m6A 3'UTR//exon       22575960
chrX:153276407-153276408(-) m6A 3'UTR//CDS//intron       -
chrX:153276542-153276543(-) m6A 3'UTR//CDS//intron       27773535
chrX:153276562-153276563(-) m6A 3'UTR//CDS//intron       27773535
chrX:153276705-153276706(-) m6A 3'UTR//intron       24209618//22575960
chrX:153276733-153276734(-) m6A 3'UTR//intron       24284625//24209618//22575960
chrX:153276756-153276757(-) m6A 3'UTR//intron       24284625//24209618//22575960
chrX:153276912-153276913(-) m6A 3'UTR//intron       24284625//24209618//25456834//27371828//27773536//22608085
chrX:153277036-153277037(-) m6A 3'UTR//intron       24284625//24209618//25456834//24981863//27773535//27371828//27773536//22575960
chrX:153277128-153277129(-) m6A 3'UTR//intron       24284625//24209618//24981863//27773535//27371828//27773536//22575960
chrX:153277349-153277350(-) m6A 3'UTR//CDS//intron       24284625//24209618//27773536//22575960
chrX:153277354-153277355(-) m6A 3'UTR//CDS//intron       24284625//24209618//27773536//22575960
chrX:153278014-153278015(-) m6A 3'UTR//CDS//intron       24284625//24209618//26404942//27773536//22575960
chrX:153278552-153278553(-) m6A 3'UTR//CDS//intron       24284625//27371828//27773536//22575960
chrX:153278591-153278592(-) m6A 3'UTR//CDS//intron       24284625//27371828//27773536//22575960
chrX:153278789-153278790(-) m6A 3'UTR//CDS//exon//intron       24284625//27371828//27773536
chrX:153279595-153279596(-) m6A 3'UTR//CDS//exon//intron       24284625//27371828//27773536
chrX:153279724-153279725(-) m6A 3'UTR//CDS//exon//intron       24284625//24981863//27371828//27773536
chrX:153279784-153279785(-) m6A exon//intron       24284625//24981863//27371828//27773536//22575960
chrX:153279792-153279793(-) m6A exon//intron       24284625//24981863//27371828//27773536//22575960
chrX:153279815-153279816(-) m6A exon//intron       24284625//24981863//27371828//27773536//22575960
chrX:153281493-153281494(-) m6A 3'UTR//CDS//exon//intron       24284625//24981863//27371828//27773536//22575960
chrX:153281535-153281536(-) m6A 3'UTR//CDS//exon//intron       24284625//27371828//27773536//22575960
chrX:153281906-153281907(-) m6A 3'UTR//CDS//exon//intron       24284625//27371828//27773536//22575960
chrX:153281912-153281913(-) m6A 3'UTR//CDS//exon//intron       24284625//27371828//27773536//22575960
chrX:153281976-153281977(-) m6A 3'UTR//CDS//exon//intron       24284625//27371828//27773536//22575960
chrX:153281982-153281983(-) m6A 3'UTR//CDS//exon//intron       24284625//27371828//27773536//22575960
chrX:153282050-153282051(-) m6A 3'UTR//CDS//exon//intron       24284625//27773536//22575960
chrX:153282408-153282409(-) m6A CDS//exon//intron       24284625//27773536//22575960
chrX:153282432-153282433(-) m6A CDS//exon//intron       24284625//27773536//22575960
chrX:153282477-153282478(-) m6A CDS//exon//intron       24284625//27773536//22575960
chrX:153283472-153283473(-) m6A CDS//exon//intron       24284625//27773536//22575960
chrX:153283547-153283548(-) m6A CDS//exon//intron       24284625//27773536//22575960
chrX:153283556-153283557(-) m6A CDS//exon//intron       24284625//27773536//22575960
chrX:153283787-153283788(-) m6A CDS//exon//intron       24284625//27773536//22575960
chrX:153284080-153284081(-) m6A CDS//exon//intron       24284625//22575960
chrX:153284129-153284130(-) Am CDS//exon//intron       -
chrX:153284268-153284269(-) m6A exon//intron       24284625
chrX:153284274-153284275(-) m6A exon//intron       24284625
chrX:153284390-153284391(-) m6A exon//intron       24284625
chrX:153284511-153284512(-) m6A CDS       24284625//27773536//22575960
chrX:153284895-153284896(-) m6A CDS       24284625//27773536//22575960
chrX:153285025-153285026(-) m6A CDS       24284625//27773536//22575960
chrX:153285336-153285337(-) m6A 5'UTR       24209618//24981863//27773536

RNA Localization:


Resource Symbol Subcellular Localization Tissue or Cell Line PMID
RNALocate IRAK1
Chromatin K562 cells     -
Cytosol Human myelogenous leukemia cell line (K-562)     21613539
Cytosol K562 cells     -
Exosome Blood     -
Nucleolus K562 cells     -
Nucleoplasm K562 cells     -
Ribosome Colon cancer cells     24393600

Interaction Network (The top 100 interactions):


Interactor1: kshv-miR-K12-9-3p
Interactor2: IRAK1

Evidence Support:


Strong-Evidence Luciferase reporter assay//RT-PCR//Western blot
Weak-Evidence Microarray
Prediction-Evidence Targetscan
Support Database ViRBase//VIRmiRNA

References:


[1]PMID 23170179 Target region 3'UTR
Source ViRBase Interactor1 expression None
Tissue or cell line 293 cells//BCBL-1 cells//BJAB cells Interactor2 expression Downregulation
Description Each method has inherent advantages and limitations, but the combination of these approaches have been successful in identifying many miRNA targets (Table 1). Table 1 List of experimentally validated cellular targets of KSHV- and EBV- encoded miRNAs. The normal cellular functions of these proteins and the effect of their knockdown during viral infection are also listed. The targets that are underlined are repressed by both KSHV- and EBV-encoded miRNAs.
[2]PMID 22896623 Target region 3'UTR
Source ViRBase//VIRmiRNA Interactor1 expression None
Tissue or cell line 293 cells//BCBL-1 cells//BJAB cells Interactor2 expression Downregulation
Description We identified interleukin-1 receptor (IL-1R)-associated kinase 1 (IRAK1) as a potential target of miR-K12-9 (miR-K9) in an array data set examining changes in cellular gene expression levels in the presence of KSHV miRNAs. Using 3'-untranslated region (3'UTR) luciferase reporter assays, we confirmed that miR-K9 and other miRNAs inhibit IRAK1 expression. In addition, IRAK1 expression is downregulated in cells transfected with miR-K9 and during de novo KSHV infection.

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