Basic Information:

  VIRBase ID:  

VHID00436049

  Virus:  

Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)

  Host:  

Homo sapiens

  Confidence Score:  

0.6002

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      kshv-miR-K12-1-5p:  MIMAT0002182          DRAM2:  NR_146303     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	kshv-miR-K12-1-5p	DRAM2
miRBase Accession/Entrez ID	MIMAT0002182	128338
Organism	Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	CORD21//PRO180//TMEM77//WWFQ154

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	DRAM2	chr1:111679487(-) A-I      intronic chr1:111675406(-) A-I      intronic chr1:111675388(-) A-I      intronic chr1:111675387(-) A-I      intronic chr1:111675397(-) A-I      intronic chr1:111679751(-) A-I      intronic chr1:111679743(-) A-I      intronic chr1:111679686(-) A-I      intronic chr1:111675932(-) A-I      intronic chr1:111679514(-) A-I      intronic chr1:111679160(-) A-I      intronic chr1:111679474(-) A-I      intronic chr1:111679763(-) A-I      intronic chr1:111675970(-) A-I      intronic chr1:111678944(-) A-I      intronic chr1:111675889(-) A-I      intronic chr1:111679484(-) A-I      intronic chr1:111675551(-) A-I      intronic chr1:111679488(-) A-I      intronic chr1:111679075(-) A-I      intronic chr1:111675975(-) A-I      intronic chr1:111679039(-) A-I      intronic chr1:111675937(-) A-I      intronic chr1:111675575(-) A-I      intronic chr1:111675876(-) A-I      intronic chr1:111675577(-) A-I      intronic chr1:111675924(-) A-I      intronic chr1:111675574(-) A-I      intronic chr1:111679447(-) A-I      intronic chr1:111679625(-) A-I      intronic chr1:111676064(-) A-I      intronic chr1:111675977(-) A-I      intronic chr1:111679155(-) A-I      intronic chr1:111675994(-) A-I      intronic chr1:111679124(-) A-I      intronic chr1:111676003(-) A-I      intronic chr1:111679065(-) A-I      intronic chr1:111675469(-) A-I      intronic chr1:111679753(-) A-I      intronic chr1:111675896(-) A-I      intronic chr1:111676004(-) A-I      intronic chr1:111679038(-) A-I      intronic chr1:111679144(-) A-I      intronic chr1:111675969(-) A-I      intronic chr1:111679479(-) A-I      intronic chr1:111676086(-) A-I      intronic chr1:111675979(-) A-I      intronic chr1:111675432(-) A-I      intronic chr1:111675988(-) A-I      intronic chr1:111679513(-) A-I      intronic chr1:111679539(-) A-I      intronic chr1:111675911(-) A-I      intronic chr1:111676073(-) A-I      intronic chr1:111679112(-) A-I      intronic chr1:111675928(-) A-I      intronic chr1:111679429(-) A-I      intronic chr1:111679086(-) A-I      intronic chr1:111675436(-) A-I      intronic chr1:111681343(-) A-I      intronic chr1:111675872(-) A-I      intronic chr1:111675946(-) A-I      intronic chr1:111676075(-) A-I      intronic chr1:111676072(-) A-I      intronic chr1:111679092(-) A-I      intronic chr1:111675364(-) A-I      intronic chr1:111675964(-) A-I      intronic chr1:111679518(-) A-I      intronic chr1:111679021(-) A-I      intronic chr1:111675948(-) A-I      intronic chr1:111675973(-) A-I      intronic chr1:111679069(-) A-I      intronic chr1:111667726(-) A-I      intronic chr1:111675376(-) A-I      intronic chr1:111675827(-) A-I      intronic chr1:111675982(-) A-I      intronic chr1:111675978(-) A-I      intronic chr1:111679145(-) A-I      intronic chr1:111679087(-) A-I      intronic chr1:111675500(-) A-I      intronic chr1:111679544(-) A-I      intronic chr1:111675938(-) A-I      intronic chr1:111675505(-) A-I      intronic chr1:111679007(-) A-I      intronic chr1:111675581(-) A-I      intronic chr1:111678995(-) A-I      intronic chr1:111676027(-) A-I      intronic

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	DRAM2	chr1:111675827(-) A-I intron -    15258596 chr1:111675872(-) A-I intron -    15258596 chr1:111675876(-) A-I intron -    15258596 chr1:111675911(-) A-I intron -    15258596 chr1:111675924(-) A-I intron -    15258596 chr1:111675928(-) A-I intron -    15258596 chr1:111675937(-) A-I intron -    15258596   //22327324 chr1:111675946(-) A-I intron -    15258596 chr1:111675948(-) A-I intron -    15258596 chr1:111675964(-) A-I intron -    15258596   //22327324 chr1:111675969(-) A-I intron -    15258596 chr1:111675973(-) A-I intron -    15258596 chr1:111675977(-) A-I intron -    15258596 chr1:111676003(-) A-I intron -    22327324 chr1:111673321(-) G-T intron -    22484847 chr1:111673619(-) C-T intron -    22484847 chr1:111675436(-) A-G intron -    22484847 chr1:111675876(-) A-G intron -    22484847 chr1:111675911(-) A-G intron -    22484847 chr1:111675937(-) A-G intron -    22484847 chr1:111675938(-) A-G intron -    22484847 chr1:111675964(-) A-G intron -    22484847 chr1:111675978(-) A-G intron -    22484847 chr1:111675982(-) A-G intron -    22484847 chr1:111675994(-) A-G intron -    22484847 chr1:111676003(-) A-G intron -    22484847 chr1:111676027(-) A-G intron -    22484847 chr1:111676064(-) A-G intron -    22484847 chr1:111676075(-) A-G intron -    22484847 chr1:111676086(-) A-G intron -    22484847 chr1:111679075(-) A-G intron -    22484847 chr1:111679079(-) G-A intron -    22484847 chr1:111679092(-) A-G intron -    22484847 chr1:111679124(-) A-G intron -    22484847 chr1:111679155(-) A-G intron -    22484847 chr1:111679513(-) A-G intron -    22484847 chr1:111679518(-) A-G intron -    22484847 chr1:111679544(-) A-G intron -    22484847 chr1:111679753(-) A-G intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	DRAM2	chr1:111660495-111660496(-) m6A 3'UTR//exon       24981863//27773535 chr1:111660534-111660535(-) m6A 3'UTR//exon       24981863//27773535//22608085 chr1:111660579-111660580(-) m6A 3'UTR//exon       24981863//27773535//22608085 chr1:111660589-111660590(-) m6A 3'UTR//exon       24981863//27773535//22608085 chr1:111660639-111660640(-) m6A 3'UTR//exon       24981863//27773535//22608085 chr1:111660807-111660808(-) m6A CDS//exon       24981863//22608085

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	DRAM2	Chromatin K562 cells     - Endoplasmic reticulum Human myelogenous leukemia cell line (K-562)     21613539 Exosome Blood     - Nucleolus K562 cells     - Nucleoplasm K562 cells     - Ribosome-free cytosol Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: kshv-miR-K12-1-5p
Interactor2: DRAM2

Evidence Support:

Weak-Evidence	Microarray
Prediction-Evidence	cRep//miRanda//Targetscan
Support Database	RepTar//ViRBase//VmiReg

References:

PMID	22174674	Target region	None
Source	RepTar//ViRBase//VmiReg	Interactor1 expression	None
Tissue or cell line	EA.hy926 cells	Interactor2 expression	Downregulation
Description	As KSHV putative direct targets we extracted transcripts that were significantly down-regulated significantly in the replicate experiments, and which contained at least one seed-match to one of the KSHV miRNAs. We identified 704 putative direct targets in DG-75 cells (Figure 3B), and 980 putative direct targets in EA.hy926 cells (Figure 3C). A complete list of putative direct targets can be found in Dataset S2 for DG-75 cells and in Dataset S3 for EA.hy926 cells. The overlap between the two datasets contained 153 putative direct targets (Dataset S4).