Basic Information:

  VIRBase ID:  

VHID00434411

  Virus:  

Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)

  Host:  

Homo sapiens

  Confidence Score:  

0.6002

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      kshv-miR-K12-1-5p:  MIMAT0002182          PDCD4:  NM_145341     

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Interactor Information:

	Interactor1	Interactor2
Symbol	kshv-miR-K12-1-5p	PDCD4
miRBase Accession/Entrez ID	MIMAT0002182	27250
Organism	Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	H731

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	PDCD4	chr10:112650922(+) A-I      intronic chr10:112636041(+) A-I      intronic chr10:112639276(+) A-I      intronic chr10:112650998(+) A-I      intronic chr10:112639286(+) A-I      intronic chr10:112635876(+) A-I      intronic chr10:112635992(+) A-I      intronic chr10:112635991(+) A-I      intronic chr10:112635900(+) A-I      intronic

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	PDCD4	chr10:112639276(+) A-G intron -    22484847 chr10:112639286(+) A-G intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	PDCD4	chr10:112631658-112631659(+) m6A 5'UTR//exon       24284625//24209618//25456834//24981863//27371828//22575960//22608085 chr10:112631664-112631665(+) m6A 5'UTR//exon       24284625//24209618//25456834//24981863//27371828//22575960//22608085 chr10:112631689-112631690(+) m6A 5'UTR//exon       24284625//24209618//25456834//24981863//27371828//22575960//22608085 chr10:112631711-112631712(+) m6A 5'UTR//exon       24284625//24209618//25456834//24981863//27371828//22575960//22608085 chr10:112631745-112631746(+) m6A 5'UTR//exon       24284625//24209618//25456834//24981863//27371828//22575960//22608085 chr10:112635819-112635820(+) m6A 5'UTR//CDS//exon//intron       24209618//24981863 chr10:112641043-112641044(+) m6A CDS//exon       22608085 chr10:112641143-112641144(+) m6A CDS//exon       24981863//22575960//22608085 chr10:112641155-112641156(+) m6A CDS//exon       24981863//22575960//22608085 chr10:112641279-112641280(+) m6A CDS//exon       24981863//22575960//22608085 chr10:112642797-112642798(+) m6A CDS//exon       24981863//27773535//22575960 chr10:112645028-112645029(+) m6A CDS//exon       22575960 chr10:112645067-112645068(+) m6A CDS//exon       22575960 chr10:112657830-112657831(+) m6A CDS//exon       26404942 chr10:112657854-112657855(+) m6A 3'UTR//exon       26404942 chr10:112658366-112658367(+) m6A 3'UTR//exon       27773535 chr10:112658388-112658389(+) m6A 3'UTR//exon       27773535 chr10:112658393-112658394(+) m6A 3'UTR//exon       27773535 chr10:112658417-112658418(+) m6A 3'UTR//exon       27773535 chr10:112658436-112658437(+) m6A 3'UTR//exon       27773535 chr10:112658449-112658450(+) m6A 3'UTR//exon       27773535 chr10:112658482-112658483(+) m6A 3'UTR       27773535 chr10:112658537-112658538(+) m6A 3'UTR       27773535 chr10:112658587-112658588(+) m6A 3'UTR       27773535 chr10:112658621-112658622(+) m6A 3'UTR       27773535

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	PDCD4	Chromatin K562 cells     - Cytosol Human myelogenous leukemia cell line (K-562)     21613539 Cytosol HCC cell line (HepG2)     - Exosome Blood     - Membrane HCC cell line (HepG2)     - Nucleus HCC cell line (HepG2)     - Ribosome HEK-293 cells     22199352

Interaction Network (The top 100 interactions):

Interactor1: kshv-miR-K12-1-5p
Interactor2: PDCD4

Evidence Support:

Weak-Evidence	Microarray
Prediction-Evidence	miRanda//Rep//Targetscan
Support Database	RepTar//ViRBase//VmiReg

References:

PMID	22174674	Target region	None
Source	RepTar//ViRBase//VmiReg	Interactor1 expression	None
Tissue or cell line	DG-75 cells	Interactor2 expression	Downregulation
Description	As KSHV putative direct targets we extracted transcripts that were significantly down-regulated significantly in the replicate experiments, and which contained at least one seed-match to one of the KSHV miRNAs. We identified 704 putative direct targets in DG-75 cells (Figure 3B), and 980 putative direct targets in EA.hy926 cells (Figure 3C). A complete list of putative direct targets can be found in Dataset S2 for DG-75 cells and in Dataset S3 for EA.hy926 cells. The overlap between the two datasets contained 153 putative direct targets (Dataset S4).