Basic Information:

  VIRBase ID:  

VHID00433130

  Virus:  

Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)

  Host:  

Homo sapiens

  Confidence Score:  

0.9797

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      kshv-miR-K12-1-5p:  MIMAT0002182          THBS1:  NM_003246     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	kshv-miR-K12-1-5p	THBS1
miRBase Accession/Entrez ID	MIMAT0002182	7057
Organism	Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	THBS//THBS-1//TSP//TSP-1//TSP1

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	THBS1	chr15:39874657-39874658(+) m6A CDS       22575960 chr15:39874711-39874712(+) m6A CDS       22575960 chr15:39874813-39874814(+) m6A CDS       24284625//22575960 chr15:39874894-39874895(+) m6A CDS       24284625 chr15:39874913-39874914(+) m6A CDS       24284625 chr15:39875850-39875851(+) m6A CDS       24284625 chr15:39875863-39875864(+) m6A CDS       24284625 chr15:39875875-39875876(+) m6A CDS       24284625 chr15:39876271-39876272(+) m6A CDS       24284625 chr15:39876278-39876279(+) m6A CDS       24284625 chr15:39876333-39876334(+) m6A CDS       24284625 chr15:39876371-39876372(+) m6A CDS       24284625 chr15:39876510-39876511(+) m6A CDS       24284625 chr15:39876590-39876591(+) m6A CDS       24284625 chr15:39878867-39878868(+) m6A intron       - chr15:39878944-39878945(+) m6A intron       - chr15:39878986-39878987(+) m6A intron       - chr15:39879684-39879685(+) m6A CDS//exon       22575960 chr15:39879690-39879691(+) m6A CDS//exon       22575960 chr15:39880246-39880247(+) m6A CDS//exon       22575960 chr15:39881457-39881458(+) m6A CDS//exon       24284625 chr15:39881463-39881464(+) m6A CDS//exon       24284625 chr15:39881533-39881534(+) m6A CDS//exon       24284625 chr15:39881551-39881552(+) m6A CDS//exon       24284625 chr15:39882038-39882039(+) m6A CDS//exon       24284625 chr15:39882153-39882154(+) m6A CDS       24284625//22575960 chr15:39882159-39882160(+) m6A CDS       24284625//22575960 chr15:39882183-39882184(+) m6A CDS       24284625 chr15:39882753-39882754(+) m6A CDS       24284625 chr15:39883150-39883151(+) m6A exon//intron       24284625 chr15:39883354-39883355(+) m6A exon//intron       24284625 chr15:39883392-39883393(+) m6A CDS//exon       24284625 chr15:39883452-39883453(+) m6A CDS//exon       24284625//22575960 chr15:39883497-39883498(+) m6A CDS//exon       24284625//22575960 chr15:39883503-39883504(+) m6A CDS//exon       24284625//22575960 chr15:39883536-39883537(+) m6A CDS//exon       24284625//26404942//22575960 chr15:39883723-39883724(+) m6A CDS//exon       24284625//26404942//22575960 chr15:39883750-39883751(+) m6A CDS//exon       24284625//22575960 chr15:39883759-39883760(+) m6A CDS//exon       24284625//22575960 chr15:39883808-39883809(+) m6A CDS//exon       24284625//22575960 chr15:39884772-39884773(+) m6A CDS       24284625//22575960 chr15:39884784-39884785(+) m6A CDS       24284625 chr15:39884841-39884842(+) m6A CDS       24284625//24981863 chr15:39884850-39884851(+) m6A CDS       24284625//24981863//26404942 chr15:39884958-39884959(+) m6A CDS       26404942 chr15:39884968-39884969(+) m6A CDS       26404942 chr15:39884994-39884995(+) m6A CDS       26404942 chr15:39886543-39886544(+) m6A CDS//exon       24209618 chr15:39886559-39886560(+) m6A CDS//exon       24284625//24209618//24981863 chr15:39886576-39886577(+) m6A CDS//exon       24284625//24209618//24981863 chr15:39887592-39887593(+) m6A 3'UTR//exon       24284625//24209618//24981863//26404942//22575960 chr15:39887603-39887604(+) m6A 3'UTR//exon       24284625//24209618//24981863//26404942//22575960 chr15:39887630-39887631(+) m6A 3'UTR//exon       24284625//24209618//25456834//24981863//26404942//22575960 chr15:39887647-39887648(+) m6A 3'UTR//exon       24284625//24209618//25456834//24981863//26404942//22575960 chr15:39887703-39887704(+) m6A 3'UTR//exon       24284625//24209618//25456834//24981863//22575960 chr15:39887749-39887750(+) m6A 3'UTR//exon       24284625//24209618//25456834//24981863//22575960 chr15:39887781-39887782(+) m6A 3'UTR       24284625//24209618//25456834//24981863//22575960 chr15:39887800-39887801(+) m6A 3'UTR       24284625//24209618//25456834//24981863//22575960 chr15:39887919-39887920(+) m6A 3'UTR       24284625//24209618//25456834//24981863//27773535//22575960 chr15:39887956-39887957(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//27773535//22575960 chr15:39887974-39887975(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//27773535//22575960 chr15:39887979-39887980(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//27773535//22575960 chr15:39888004-39888005(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//27773535//22575960 chr15:39888079-39888080(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//27773535//22575960 chr15:39888123-39888124(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//22575960 chr15:39888207-39888208(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//22575960 chr15:39888213-39888214(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//22575960 chr15:39888225-39888226(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//22575960 chr15:39888291-39888292(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//22575960 chr15:39888391-39888392(+) m6A 3'UTR       24284625//24209618 chr15:39888638-39888639(+) m6A 3'UTR       24981863 chr15:39888656-39888657(+) m6A 3'UTR       24981863 chr15:39890382-39890383(+) m6A 3'UTR       24981863 chr15:39890750-39890751(+) m6A 3'UTR       24981863 chr15:39890774-39890775(+) m6A 3'UTR       24981863 chr15:39890835-39890836(+) m6A 3'UTR       24981863 chr15:39890873-39890874(+) m6A 3'UTR       24981863

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	THBS1	Cytosol HeLa-S3 cells     - Exosome Blood     -

Interaction Network (The top 100 interactions):

Interactor1: kshv-miR-K12-1-5p
Interactor2: THBS1

Evidence Support:

Strong-Evidence	Luciferase reporter assay//Northern blot//qRT-PCR
Weak-Evidence	HITS-CLIP
Prediction-Evidence	miRanda
Support Database	ViRBase//VIRmiRNA//VmiReg

References:

[1]PMID	17381317	Target region	3'UTR
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	BCBL1 cells	Interactor2 expression	None
Description	Recovery of previously experimentally verified targets of KSHV miRNAs by Ago-HITS-CLIP.

[2]PMID	20580307	Target region	3'UTR
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	293 cells//BJAB cells	Interactor2 expression	Downregulation
Description	From the targets identified to date (Table 1, Figure 1) it is apparent that viral miRNAs play an important role in immune evasion by inhibiting immune surveillance and extending the life of the infected host cell. Table 1 Immunomodulatory viral miRNAs.

[3]PMID	21152115	Target region	3'UTR
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	293 cells//BJAB cells	Interactor2 expression	Downregulation
Description	KSHV miR-K12-1, miR-K12-3-3p, miR-K12-6-3p, and miR-K12-11 reduce the expression of THBS1(thrombospondin 1) by interaction with the THBS1 3'UTR.

[4]PMID	17500590	Target region	3'UTR
Source	ViRBase//VIRmiRNA//VmiReg	Interactor1 expression	None
Tissue or cell line	293 cells//BJAB cells	Interactor2 expression	Downregulation
Description	We performed luciferase de-repression assays by co-transfection of the THBS1 3'UTR reporter and 2'OMe RNAs specific to individual KSHV miRNAs. Our results demonstrate that THBS1 is targeted by multiple KSHV miRNAs; in particular, miR-K12-1, miR-K12-3-3p, miR-K12-6-3p, and miR-K12-11 lead to strong de-repression of the reporter.

[5]PMID	21616184	Target region	3'UTR
Source	ViRBase//VIRmiRNA//VmiReg	Interactor1 expression	None
Tissue or cell line	293 cells//BJAB cells	Interactor2 expression	Downregulation
Description	Currently, the validated targets of KSHV miRNAs (Table 1) represent a collection of genes identified by unbiased methods or chosen by previously identified functions.Table 1 KSHVmiRNA validated targets. All targets have been validated using ectopic expression of miRNAs to demonstrate that a luciferase reporter or ectopic reporter are repressed. Additional validations include(1) exogenous reporters have been mutated to disrupt targeting by a ectopic miRNA,(2) ectopic miRNA suppresses endogenous target mRNA and/or protein levels,(3) de novo infection with KSHV represses endogenous target expression,(4) miRNA inhibitors and/or mutant virus display derepression of endogenous target mRNA and/or protein levels, and(5) repression of miRNA target is observed in KSHV clinical samples.