Basic Information:

  VIRBase ID:  

VHID00432690

  Virus:  

Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)

  Host:  

Homo sapiens

  Confidence Score:  

0.9526

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      kshv-miR-K12-1-5p:  MIMAT0002182          NFKB1:  NM_001319226     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	kshv-miR-K12-1-5p	NFKB1
miRBase Accession/Entrez ID	MIMAT0002182	4790
Organism	Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	CVID12//EBP-1//KBF1//NF-kappa-B1//NF-kappaB//NF-kappabeta//NF-kB//NF-kB1//NFkappaB//NFKB-p105//NFKB-p50

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	NFKB1	chr4:103523438(+) A-I      intronic chr4:103523449(+) A-I      intronic chr4:103523459(+) A-I      intronic chr4:103493795(+) A-I      intronic chr4:103492977(+) A-I      intronic chr4:103492953(+) A-I      intronic chr4:103491504(+) A-I      intronic chr4:103491499(+) A-I      intronic chr4:103491467(+) A-I      intronic chr4:103465253(+) A-I      intronic chr4:103524504(+) A-I      intronic chr4:103524508(+) A-I      intronic chr4:103523433(+) A-I      intronic chr4:103524404(+) A-I      intronic chr4:103524369(+) A-I      intronic chr4:103524359(+) A-I      intronic chr4:103524347(+) A-I      intronic chr4:103524330(+) A-I      intronic chr4:103523603(+) A-I      intronic chr4:103481082(+) A-I      intronic chr4:103479949(+) A-I      intronic chr4:103479851(+) A-I      intronic chr4:103433632(+) A-I      intronic chr4:103433605(+) A-I      intronic chr4:103432839(+) A-I      intronic chr4:103432582(+) A-I      intronic chr4:103480975(+) A-I      intronic chr4:103524467(+) A-I      intronic chr4:103433606(+) A-I      intronic chr4:103479909(+) A-I      intronic chr4:103523426(+) A-I      intronic chr4:103432753(+) A-I      intronic chr4:103432590(+) A-I      intronic chr4:103432816(+) A-I      intronic chr4:103523576(+) A-I      intronic chr4:103432576(+) A-I      intronic chr4:103479947(+) A-I      intronic chr4:103432560(+) A-I      intronic chr4:103524281(+) A-I      intronic chr4:103432739(+) A-I      intronic chr4:103479811(+) A-I      intronic chr4:103433631(+) A-I      intronic chr4:103433610(+) A-I      intronic chr4:103479934(+) A-I      intronic chr4:103479920(+) A-I      intronic chr4:103480821(+) A-I      intronic chr4:103433724(+) A-I      intronic chr4:103480827(+) A-I      intronic chr4:103481040(+) A-I      intronic chr4:103435067(+) A-I      intronic chr4:103480989(+) A-I      intronic chr4:103523432(+) A-I      intronic chr4:103481050(+) A-I      intronic chr4:103479831(+) A-I      intronic

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	NFKB1	chr4:103480975(+) A-I intron -    22327324 chr4:103491632(+) A-G intron -    22484847 chr4:103491738(+) A-G intron -    22484847 chr4:103493713(+) A-G intron -    22484847 chr4:103493734(+) A-G intron -    22484847 chr4:103432560(+) A-G intron -    22484847 chr4:103432576(+) A-G intron -    22484847 chr4:103432590(+) A-G intron -    22484847 chr4:103432646(+) A-G intron -    22484847 chr4:103432650(+) A-G intron -    22484847 chr4:103432753(+) A-G intron -    22484847 chr4:103432816(+) A-G intron -    22484847 chr4:103433557(+) A-G intron -    22484847 chr4:103433606(+) A-G intron -    22484847 chr4:103433610(+) A-G intron -    22484847 chr4:103433618(+) A-G intron -    22484847 chr4:103433623(+) A-G intron -    22484847 chr4:103433631(+) A-G intron -    22484847 chr4:103435067(+) A-G intron -    22484847 chr4:103479811(+) A-G intron -    22484847 chr4:103479831(+) A-G intron -    22484847 chr4:103479877(+) A-G intron -    22484847 chr4:103479920(+) A-G intron -    22484847 chr4:103479934(+) A-G intron -    22484847 chr4:103479937(+) A-G intron -    22484847 chr4:103480975(+) A-G intron -    22484847 chr4:103480989(+) A-G intron -    22484847 chr4:103523426(+) A-G intron -    22484847 chr4:103523430(+) A-G intron -    22484847 chr4:103523576(+) A-G intron -    22484847 chr4:103523638(+) A-G intron -    22484847 chr4:103524281(+) A-G intron -    22484847 chr4:103524467(+) A-G intron -    22484847 chr4:103530591(+) A-T intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	NFKB1	chr4:103422502-103422503(+) m6A 5'UTR       24981863 chr4:103517354-103517355(+) m6A CDS//exon       26404942 chr4:103517363-103517364(+) m6A CDS//exon       26404942 chr4:103517386-103517387(+) m6A CDS//exon       26404942 chr4:103517461-103517462(+) m6A CDS//exon       26404942 chr4:103537602-103537603(+) m6A CDS       24284625 chr4:103537634-103537635(+) m6A CDS       24284625//24981863//26404942 chr4:103537648-103537649(+) m6A CDS       24284625//24981863//26404942 chr4:103537729-103537730(+) m6A CDS       24284625//24981863//26404942//22608085 chr4:103537779-103537780(+) m6A 3'UTR       24284625//24981863//26404942//22608085 chr4:103537815-103537816(+) m6A 3'UTR       24284625//24981863//26404942//22608085 chr4:103537886-103537887(+) m6A 3'UTR       24284625//26404942

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	NFKB1	Cytosol Human myelogenous leukemia cell line (K-562)     21613539 Exosome Blood     - Nucleus HeLa-S3 cells     - Ribosome HEK-293 cells     22199352 Ribosome Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: kshv-miR-K12-1-5p
Interactor2: NFKB1

Evidence Support:

Strong-Evidence	Luciferase reporter assay//qRT-PCR//Western blot
Support Database	ViRBase

References:

[1]PMID	20081837	Target region	None
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	293T cells//BCP-1 cells	Interactor2 expression	Downregulation
Description	We further inhibited the function of miR-K1 in KSHV-infected PEL cells using a locked nucleic acids/DNA-mixed oligonucleotide (miR-K1 suppressor), which resulted in the reduction of NF-kaaB activity by 40% (Fig.4c).

[2]PMID	21152115	Target region	None
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	293T cells//BCP-1 cells	Interactor2 expression	Upregulation
Description	More studies from recent reports indicate miR-K12-5, miR-K12-9 and miR-K12-10 repression of Bcl-2-associated transcription factor 1, an apoptosis-inducing factor (238), miR-K12-3 reduction of LRRC8D (leucine rich repeat containing 8 family, member D), a leucin-rich type III transmembrane protein involved in proliferation and activation of lymphocytes, NHP2L1 (non-histone chromosome protein 2-like 1) (239), and a transcription factor NFIB (240), miR-K12-1 inhibition of p21 241 and NFkappaB inhibitor IkappaBalpha (242), miR-K12-4-3p suppression of GEMIN8 (239), miR-K12-10a reduction of TWEAKR (243), miR-K12-1, -6, and -11 decrease of MAF (musculoaponeurotic fibrosarcoma oncogene homolog) (244), K12-4-5p targeting retinoblastoma (Rb)-like protein 2 (Rbl2) and miR-K12-5 and -9*suppressing ORF50 mRNA (245,246).