Detail Information

Basic Information:


  VIRBase ID:  

VHID00432568

  Virus:  

Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)

  Host:  

Homo sapiens

  Confidence Score:  

0.9812

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      kshv-miR-K12-1-5p:           MAF:      

      *It may take a few minutes to display results.

Interactor Information:


Interactor1 Interactor2
Symbol kshv-miR-K12-1-5p MAF
miRBase
Accession/Entrez ID		 MIMAT0002182 4094
Organism Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV) Homo sapiens
Category miRNA mRNA
Alias - AYGRP//c-MAF//CCA4//CTRCT21

RNA Modification:


Resource Symbol ModificationPosition Type Genomic Context PMID
RMBase MAF
chr16:79630602-79630603(-) m6A 3'UTR//intron       27773535
chr16:79630674-79630675(-) m6A 3'UTR//intron       27773535
chr16:79630775-79630776(-) m6A 3'UTR//intron       27773535//22608085
chr16:79630831-79630832(-) m6A 3'UTR//intron       27773535//22608085
chr16:79630933-79630934(-) m6A 3'UTR//intron       27773535//22608085
chr16:79631028-79631029(-) m6A 3'UTR//intron       27773535
chr16:79631298-79631299(-) m6A 3'UTR//intron       27773535//22608085
chr16:79631354-79631355(-) m6A 3'UTR//intron       24981863//27773535//22608085
chr16:79631400-79631401(-) m6A 3'UTR//intron       24981863//27773535//22608085
chr16:79631410-79631411(-) m6A 3'UTR//intron       24284625//24209618//24981863//27773535//22608085
chr16:79631484-79631485(-) m6A 3'UTR//intron       24284625//24209618//24981863//27773535//22608085
chr16:79631504-79631505(-) m6A 3'UTR//intron       24284625//24209618//24981863//27773535//22608085
chr16:79631555-79631556(-) m6A 3'UTR//intron       24284625//24209618//24981863//27773535//22608085
chr16:79631560-79631561(-) m6A 3'UTR//intron       24284625//24209618//24981863//27773535//22608085
chr16:79631619-79631620(-) m6A 3'UTR//intron       24209618//24981863//27773535//22608085
chr16:79631683-79631684(-) m6A 3'UTR//intron       24209618//24981863//27773535//22608085
chr16:79631720-79631721(-) m6A 3'UTR//intron       24209618//24981863//27773535//22608085
chr16:79631736-79631737(-) m6A 3'UTR//intron       24209618//24981863//27773535//22608085
chr16:79631754-79631755(-) m6A 3'UTR//intron       24209618//24981863//27773535//22608085
chr16:79631809-79631810(-) m6A 3'UTR//intron       24209618//24981863//27773535//22608085
chr16:79631906-79631907(-) m6A 3'UTR//intron       24209618//24981863//27773535//22608085
chr16:79631982-79631983(-) m6A 3'UTR//intron       22608085
chr16:79632217-79632218(-) m6A 3'UTR//intron       24981863//27773535
chr16:79632328-79632329(-) m6A 3'UTR//intron       24981863//27773535
chr16:79632435-79632436(-) m6A 3'UTR//intron       24209618//24981863//27773535//22608085
chr16:79632522-79632523(-) m6A 3'UTR//intron       27773535
chr16:79632837-79632838(-) m6A CDS       24284625//24209618//25456834//24981863//27371828//27773536//22608085
chr16:79632860-79632861(-) m6A CDS       24284625//24209618//25456834//24981863//27371828//27773536//22608085
chr16:79632906-79632907(-) m6A CDS       24209618//24981863//27371828//27773536//22608085
chr16:79632916-79632917(-) m6A CDS       24209618//24981863//27371828//27773536//22608085
chr16:79632975-79632976(-) m6A CDS       24209618//27371828//27773536//22608085
chr16:79633494-79633495(-) m6A CDS       24981863//27371828//27773536//22608085
chr16:79633530-79633531(-) m6A CDS       24981863//27371828//27773536//22608085
chr16:79633684-79633685(-) m6A CDS       24209618//24981863//27773535//27371828//27773536//22608085
chr16:79633694-79633695(-) m6A CDS       24209618//24981863//27773535//27371828//27773536//22608085
chr16:79633787-79633788(-) m6A CDS       24209618//24981863//27371828//27773536//22608085
chr16:79634300-79634301(-) m6A 5'UTR       24209618//24981863//27773535//27773536//22608085
chr16:79634476-79634477(-) m6A 5'UTR       24981863//27773535//22608085
chr16:79634481-79634482(-) m6A 5'UTR       24981863//27773535//22608085
chr16:79634520-79634521(-) m6A 5'UTR       24981863//27773535//22608085

RNA Localization:


Resource Symbol Subcellular Localization Tissue or Cell Line PMID
RNALocate MAF
Exosome Blood     -

Interaction Network (The top 100 interactions):


Interactor1: kshv-miR-K12-1-5p
Interactor2: MAF

Evidence Support:


Strong-Evidence Luciferase reporter assay//RT-PCR//Western blot
Weak-Evidence Microarray
Prediction-Evidence cRep//miRanda//PITA//Targetscan
Support Database RepTar//ViRBase//VIRmiRNA//VmiReg

References:


[1]PMID 21152115 Target region 3'UTR
Source RepTar//ViRBase Interactor1 expression None
Tissue or cell line 293T cells//HeLa cells Interactor2 expression Downregulation
Description More studies from recent reports indicate miR-K12-5, miR-K12-9 and miR-K12-10 repression of Bcl-2-associated transcription factor 1, an apoptosis-inducing factor (238), miR-K12-3 reduction of LRRC8D (leucine rich repeat containing 8 family, member D), a leucin-rich type III transmembrane protein involved in proliferation and activation of lymphocytes, NHP2L1 (non-histone chromosome protein 2-like 1) (239), and a transcription factor NFIB (240), miR-K12-1 inhibition of p21 241 and NFkappaB inhibitor IkappaBalpha (242), miR-K12-4-3p suppression of GEMIN8 (239), miR-K12-10a reduction of TWEAKR (243), miR-K12-1, -6, and -11 decrease of MAF (musculoaponeurotic fibrosarcoma oncogene homolog) (244), K12-4-5p targeting retinoblastoma (Rb)-like protein 2 (Rbl2) and miR-K12-5 and -9*suppressing ORF50 mRNA (245,246).
[2]PMID 21277611 Target region 3'UTR
Source RepTar//ViRBase Interactor1 expression None
Tissue or cell line 293T cells//HeLa cells Interactor2 expression Downregulation
Description Analysis of the negatively regulated transcripts with the PITA algorithm (Kertesz et al., 2007) led to the identification of the cellular transcription factor MAF (musculoaponeurotic fibrosarcoma oncogene homolog), which was experimentally confirmed to be a direct target of several KSHV-encoded miRNAs.
[3]PMID 20080955 Target region 3'UTR
Source RepTar//ViRBase//VIRmiRNA//VmiReg Interactor1 expression None
Tissue or cell line 293T cells//HeLa cells Interactor2 expression Downregulation
Description Site-directed mutagenesis of KSHV miRNA predicted target sites in the MAF 3'UTR abolished silencing (Fig.3D; Supplemental Fig.2D), confirming that miR-K12-1, miR-K12-6-5p, and miR-K12-11 regulate MAF by interacting with the 3'UTR.
[4]PMID 21616184 Target region 3'UTR
Source RepTar//ViRBase//VIRmiRNA//VmiReg Interactor1 expression None
Tissue or cell line 293T cells//HeLa cells Interactor2 expression Downregulation
Description Currently, the validated targets of KSHV miRNAs (Table 1) represent a collection of genes identified by unbiased methods or chosen by previously identified functions.Table 1 KSHVmiRNA validated targets. All targets have been validated using ectopic expression of miRNAs to demonstrate that a luciferase reporter or ectopic reporter are repressed. Additional validations include(1) exogenous reporters have been mutated to disrupt targeting by a ectopic miRNA,(2) ectopic miRNA suppresses endogenous target mRNA and/or protein levels,(3) de novo infection with KSHV represses endogenous target expression,(4) miRNA inhibitors and/or mutant virus display derepression of endogenous target mRNA and/or protein levels, and(5) repression of miRNA target is observed in KSHV clinical samples.

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