Detail Information

Basic Information:


  VIRBase ID:  

VHID00426616

  Virus:  

Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)

  Host:  

Homo sapiens

  Confidence Score:  

0.9612

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      kshv-miR-K12-10b:           BCLAF1:      

      *It may take a few minutes to display results.

Interactor Information:


Interactor1 Interactor2
Symbol kshv-miR-K12-10b BCLAF1
miRBase
Accession/Entrez ID		 MIMAT0002180 9774
Organism Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV) Homo sapiens
Category miRNA mRNA
Alias - bK211L9.1//BTF

RNA Editing:


Resource Symbol Editing Position Change Genetic Region
RADAR BCLAF1
chr6:136607070(-) A-I      intronic
chr6:136585549(-) A-I      intronic

RNA Modification:


Resource Symbol ModificationPosition Type Genomic Context PMID
RMBase BCLAF1
chr6:136578085-136578086(-) m6A 3'UTR       27773535
chr6:136580764-136580765(-) m6A 3'UTR//intron       24981863
chr6:136580775-136580776(-) m6A 3'UTR//intron       24981863
chr6:136580780-136580781(-) m6A 3'UTR//intron       24981863
chr6:136580790-136580791(-) m6A 3'UTR//intron       24981863
chr6:136580803-136580804(-) m6A 3'UTR//intron       24981863
chr6:136580852-136580853(-) m6A 3'UTR//intron       24981863
chr6:136582081-136582082(-) m6A 3'UTR//exon//intron       27773535
chr6:136582215-136582216(-) m6A 3'UTR//exon       24981863//28052920//27773535//22608085
chr6:136582421-136582422(-) m6A 3'UTR//CDS//exon       24209618//24981863//28052920//27773535//27371828//22608085
chr6:136582449-136582450(-) m6A 3'UTR//CDS//exon       24209618//24981863//28052920//27773535//27371828//22608085
chr6:136582497-136582498(-) m6A 3'UTR//CDS//exon       24209618//24981863//28052920//27773535//27371828//22608085
chr6:136588223-136588224(-) m6A 3'UTR//CDS//exon//intron       24981863//27773535//27371828//22608085
chr6:136588251-136588252(-) m6A 3'UTR//CDS//exon//intron       24981863//27773535//27371828
chr6:136588267-136588268(-) m6A 3'UTR//CDS//exon//intron       24981863//27773535//27371828
chr6:136589304-136589305(-) m6A 3'UTR//CDS//exon       27773535
chr6:136589361-136589362(-) m6A 3'UTR//CDS//exon       27773535
chr6:136589473-136589474(-) m6A 3'UTR//CDS//exon//intron       24981863//27773535
chr6:136589709-136589710(-) m6A exon//intron       27773535
chr6:136589731-136589732(-) m6A exon//intron       27773535
chr6:136589736-136589737(-) m6A exon//intron       27773535
chr6:136589764-136589765(-) m6A exon//intron       27773535
chr6:136590561-136590562(-) m6A exon//intron       24981863
chr6:136590617-136590618(-) m6A 3'UTR//CDS//exon       24981863//27773535
chr6:136594254-136594255(-) m6A 3'UTR//CDS//exon       27773535
chr6:136596818-136596819(-) m6A 3'UTR//CDS       27773535
chr6:136597142-136597143(-) m6A CDS//intron       24284625//24981863//27773535//22608085
chr6:136597182-136597183(-) m6A CDS//intron       24284625//24981863//27773535//22608085
chr6:136597217-136597218(-) m6A CDS//intron       24284625//24209618//24981863//28052920//27773535//22608085
chr6:136597272-136597273(-) m6A CDS//intron       24284625//24209618//24981863//28052920//27773535//22608085
chr6:136597282-136597283(-) m6A CDS//intron       24284625//24209618//24981863//28052920//27773535//22575960//22608085
chr6:136597357-136597358(-) m6A CDS//intron       24284625//24209618//24981863//28052920//27773535//27371828//22575960//22608085
chr6:136597435-136597436(-) m6A CDS//intron       24284625//24209618//24981863//28052920//27773535//27371828//22575960//22608085
chr6:136597449-136597450(-) m6A CDS//intron       24284625//24209618//24981863//28052920//27773535//27371828//22575960//22608085
chr6:136597489-136597490(-) m6A CDS//intron       24284625//24981863//27773535//27371828//22608085
chr6:136599021-136599022(-) m6A CDS       24284625//27773535//27371828
chr6:136599030-136599031(-) m6A CDS       24284625//27773535//27371828
chr6:136599108-136599109(-) m6A CDS       27773535
chr6:136599442-136599443(-) m6A CDS       24284625//25456834//24981863//28052920//27773535//22608085
chr6:136599460-136599461(-) m6A CDS       24284625//25456834//24981863//28052920//27773535//22608085
chr6:136599502-136599503(-) m6A CDS       24284625//25456834//24981863//28052920//27773535//22608085
chr6:136599526-136599527(-) m6A CDS       24284625//25456834//24981863//28052920//27773535//22608085
chr6:136603756-136603757(-) m6A 5'UTR//exon       24284625
chr6:136610869-136610870(-) m6A 5'UTR//exon       24284625//24981863

RNA Localization:


Resource Symbol Subcellular Localization Tissue or Cell Line PMID
RNALocate BCLAF1
Chromatin HCC cell line (HepG2)|K562 cells     -
Cytosol Human myelogenous leukemia cell line (K-562)     21613539
Cytosol HCC cell line (HepG2)|K562 cells     -
Exosome Blood     -
Nucleolus K562 cells     -
Nucleoplasm HCC cell line (HepG2)|K562 cells     -
Nucleus Colon cancer cells     24393600
Nucleus HCC cell line (HepG2)|HeLa-S3 cells|K562 cells     -
Ribosome HEK-293 cells     22199352

Interaction Network (The top 100 interactions):


Interactor1: kshv-miR-K12-10b
Interactor2: BCLAF1

Evidence Support:


Strong-Evidence Luciferase reporter assay//Western blot
Weak-Evidence Microarray//PAR-CLIP
Prediction-Evidence miRanda//PARalyzer//Targetscan
Support Database ViRBase//VmiReg

References:


[1]PMID 20580307 Target region 3'UTR
Source ViRBase Interactor1 expression None
Tissue or cell line 293 cells//BCBL-1 cells//BCBL-3 cells//BJAB cells//HUVECs Interactor2 expression None
Description From the targets identified to date (Table 1, Figure 1) it is apparent that viral miRNAs play an important role in immune evasion by inhibiting immune surveillance and extending the life of the infected host cell. Table 1 Immunomodulatory viral miRNAs.
[2]PMID 21277611 Target region 3'UTR
Source ViRBase Interactor1 expression None
Tissue or cell line 293 cells//BCBL-1 cells//BCBL-3 cells//BJAB cells//HUVECs Interactor2 expression Downregulation
Description An overview of known targets of cellular or viral targets is given in tables 2 and 3.Table 2 Cellular Targets of viral miRNAs.Table 3 Viral Targets of viral miRNAs.
[3]PMID 23170179 Target region 3'UTR
Source ViRBase Interactor1 expression None
Tissue or cell line 293 cells//BCBL-1 cells//BCBL-3 cells//BJAB cells//HUVECs Interactor2 expression Downregulation
Description Each method has inherent advantages and limitations, but the combination of these approaches have been successful in identifying many miRNA targets (Table 1). Table 1 List of experimentally validated cellular targets of KSHV- and EBV- encoded miRNAs. The normal cellular functions of these proteins and the effect of their knockdown during viral infection are also listed. The targets that are underlined are repressed by both KSHV- and EBV-encoded miRNAs.
[4]PMID 19098914 Target region 3'UTR
Source ViRBase//VmiReg Interactor1 expression None
Tissue or cell line 293 cells//BCBL-1 cells//BCBL-3 cells//BJAB cells//HUVECs Interactor2 expression Downregulation
Description Fig.3a shows that, in addition to miR-K5, several other KSHV miRs down-regulated this protein reproducibly-notably, miRs K9, K10a and K10b. Each of these miRNAs was tested for the ability to downregulate the LUC-BCLAF1 3'UTR reporter construct (Fig.3b).
[5]PMID 21616184 Target region 3'UTR
Source ViRBase//VmiReg Interactor1 expression None
Tissue or cell line 293 cells//BCBL-1 cells//BCBL-3 cells//BJAB cells//HUVECs Interactor2 expression Downregulation
Description Currently, the validated targets of KSHV miRNAs (Table 1) represent a collection of genes identified by unbiased methods or chosen by previously identified functions.Table 1 KSHVmiRNA validated targets. All targets have been validated using ectopic expression of miRNAs to demonstrate that a luciferase reporter or ectopic reporter are repressed. Additional validations include(1) exogenous reporters have been mutated to disrupt targeting by a ectopic miRNA,(2) ectopic miRNA suppresses endogenous target mRNA and/or protein levels,(3) de novo infection with KSHV represses endogenous target expression,(4) miRNA inhibitors and/or mutant virus display derepression of endogenous target mRNA and/or protein levels, and(5) repression of miRNA target is observed in KSHV clinical samples.
[6]PMID 22100165 Target region 3'UTR
Source ViRBase//VmiReg Interactor1 expression None
Tissue or cell line PEL cells Interactor2 expression None
Description Our dataset also confirmed 12 out of 29 validated KSHV miRNA targets with expression in our dataset(>40%). Confirmed interactions include those with BACH1, FOS, CDKN1A(p21), TNFRSF12A(TWEAKR), RAD21 and RBL2 mRNAs, whose regulation had previously been validated at the level of protein expression. A list summarizing the recovery of previously published targets of the KSHV and EBV miRNAs is presented in Table S9. NIHMS333942-supplement-01 Table S9 Recovery of previously published targets for KSHV or EBV miRNAs.

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