Basic Information:

  VIRBase ID:  

VHID00426248

  Virus:  

Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)

  Host:  

Homo sapiens

  Confidence Score:  

0.8847

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      kshv-miR-K12-10b:  MIMAT0002180          PKN2:  NM_006256     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	kshv-miR-K12-10b	PKN2
miRBase Accession/Entrez ID	MIMAT0002180	5586
Organism	Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	Pak-2//PAK2//PRK2//PRKCL2//PRO2042//STK7

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	PKN2	chr1:89289229(+) A-I      intronic chr1:89288792(+) A-I      intronic chr1:89176577(+) A-I      intronic chr1:89176565(+) A-I      intronic chr1:89176560(+) A-I      intronic chr1:89176501(+) A-I      intronic chr1:89176451(+) A-I      intronic chr1:89258579(+) A-I      intronic chr1:89210203(+) A-I      intronic chr1:89208718(+) A-I      intronic chr1:89289244(+) A-I      intronic chr1:89230706(+) A-I      intronic chr1:89230705(+) A-I      intronic chr1:89210243(+) A-I      intronic chr1:89230701(+) A-I      intronic chr1:89179084(+) A-I      intronic chr1:89176757(+) A-I      intronic chr1:89176741(+) A-I      intronic chr1:89176718(+) A-I      intronic chr1:89176712(+) A-I      intronic chr1:89176711(+) A-I      intronic chr1:89176599(+) A-I      intronic chr1:89256972(+) A-I      intronic chr1:89288326(+) A-I      intronic chr1:89210285(+) A-I      intronic chr1:89210288(+) A-I      intronic chr1:89288761(+) A-I      intronic chr1:89282113(+) A-I      intronic chr1:89242267(+) A-I      intronic chr1:89242303(+) A-I      intronic chr1:89244471(+) A-I      intronic chr1:89244498(+) A-I      intronic chr1:89244702(+) A-I      intronic chr1:89249840(+) A-I      intronic chr1:89249968(+) A-I      intronic chr1:89249973(+) A-I      intronic chr1:89250026(+) A-I      intronic chr1:89254295(+) A-I      intronic chr1:89254367(+) A-I      intronic chr1:89255076(+) A-I      intronic chr1:89255145(+) A-I      intronic chr1:89255162(+) A-I      intronic chr1:89255225(+) A-I      intronic chr1:89255328(+) A-I      intronic chr1:89255330(+) A-I      intronic chr1:89272045(+) A-I      intronic chr1:89274226(+) A-I      intronic chr1:89275336(+) A-I      intronic chr1:89275439(+) A-I      intronic chr1:89278339(+) A-I      intronic chr1:89281720(+) A-I      intronic chr1:89281749(+) A-I      intronic chr1:89281755(+) A-I      intronic chr1:89282095(+) A-I      intronic chr1:89282110(+) A-I      intronic chr1:89274128(+) A-I      intronic chr1:89282115(+) A-I      intronic chr1:89282132(+) A-I      intronic chr1:89282139(+) A-I      intronic chr1:89282150(+) A-I      intronic chr1:89286066(+) A-I      intronic chr1:89286096(+) A-I      intronic chr1:89288482(+) A-I      intronic chr1:89288523(+) A-I      intronic chr1:89288652(+) A-I      intronic chr1:89288737(+) A-I      intronic chr1:89288757(+) A-I      intronic chr1:89289183(+) A-I      intronic chr1:89289199(+) A-I      intronic chr1:89289336(+) A-I      intronic chr1:89242246(+) A-I      intronic chr1:89153886(+) A-I      intronic chr1:89153950(+) A-I      intronic chr1:89155374(+) A-I      intronic chr1:89155415(+) A-I      intronic chr1:89155439(+) A-I      intronic chr1:89155449(+) A-I      intronic chr1:89156847(+) A-I      intronic chr1:89156886(+) A-I      intronic chr1:89167817(+) A-I      intronic chr1:89227230(+) A-I      intronic chr1:89229105(+) A-I      intronic chr1:89229135(+) A-I      intronic chr1:89185740(+) A-I      intronic chr1:89185894(+) A-I      intronic chr1:89185939(+) A-I      intronic chr1:89229169(+) A-I      intronic chr1:89229170(+) A-I      intronic chr1:89229196(+) A-I      intronic chr1:89229197(+) A-I      intronic chr1:89229202(+) A-I      intronic chr1:89189230(+) A-I      intronic chr1:89229205(+) A-I      intronic chr1:89238997(+) A-I      intronic chr1:89239006(+) A-I      intronic chr1:89239069(+) A-I      intronic chr1:89239100(+) A-I      intronic chr1:89241577(+) A-I      intronic chr1:89241684(+) A-I      intronic chr1:89241712(+) A-I      intronic chr1:89242149(+) A-I      intronic chr1:89242151(+) A-I      intronic chr1:89242161(+) A-I      intronic chr1:89242247(+) A-I      intronic chr1:89242252(+) A-I      intronic chr1:89208616(+) A-I      intronic chr1:89208627(+) A-I      intronic chr1:89208679(+) A-I      intronic chr1:89208805(+) A-I      intronic chr1:89223676(+) A-I      intronic chr1:89223679(+) A-I      intronic chr1:89223681(+) A-I      intronic chr1:89227197(+) A-I      intronic chr1:89227209(+) A-I      intronic chr1:89153817(+) A-I      intronic chr1:89281805(+) A-I      intronic chr1:89282029(+) A-I      intronic chr1:89288379(+) A-I      intronic chr1:89188381(+) A-I      intronic chr1:89275374(+) A-I      intronic chr1:89285998(+) A-I      intronic chr1:89242261(+) A-I      intronic chr1:89230873(+) A-I      intronic chr1:89210307(+) A-I      intronic chr1:89214533(+) A-I      intronic chr1:89281702(+) A-I      intronic chr1:89286109(+) A-I      intronic chr1:89282032(+) A-I      intronic chr1:89210233(+) A-I      intronic chr1:89231534(+) A-I      intronic chr1:89210204(+) A-I      intronic chr1:89223604(+) A-I      intronic chr1:89254402(+) A-I      intronic chr1:89288754(+) A-I      intronic chr1:89166863(+) A-I      intronic chr1:89255226(+) A-I      intronic chr1:89281607(+) A-I      intronic chr1:89230749(+) A-I      intronic chr1:89189271(+) A-I      intronic chr1:89223636(+) A-I      intronic chr1:89240682(+) A-I      intronic chr1:89289236(+) A-I      intronic chr1:89189286(+) A-I      intronic chr1:89227181(+) A-I      intronic chr1:89255164(+) A-I      intronic chr1:89288646(+) A-I      intronic chr1:89255333(+) A-I      intronic chr1:89239242(+) A-I      intronic chr1:89286123(+) A-I      intronic chr1:89282007(+) A-I      intronic chr1:89156983(+) A-I      intronic chr1:89288837(+) A-I      intronic chr1:89288858(+) A-I      intronic chr1:89188359(+) A-I      intronic chr1:89254375(+) A-I      intronic chr1:89281622(+) A-I      intronic chr1:89278358(+) A-I      intronic chr1:89230745(+) A-I      intronic chr1:89278241(+) A-I      intronic chr1:89289438(+) A-I      intronic chr1:89202245(+) A-I      intronic chr1:89268223(+) A-I      intronic chr1:89282182(+) A-I      intronic chr1:89275363(+) A-I      intronic chr1:89208822(+) A-I      intronic chr1:89167825(+) A-I      intronic chr1:89284348(+) A-I      intronic chr1:89239324(+) A-I      intronic chr1:89255090(+) A-I      intronic chr1:89286058(+) A-I      intronic chr1:89157016(+) A-I      intronic chr1:89189208(+) A-I      intronic chr1:89289308(+) A-I      intronic chr1:89288462(+) A-I      intronic chr1:89288447(+) A-I      intronic chr1:89229052(+) A-I      intronic chr1:89281676(+) A-I      intronic chr1:89268109(+) A-I      intronic chr1:89268244(+) A-I      intronic chr1:89282037(+) A-I      intronic chr1:89274169(+) A-I      intronic chr1:89170384(+) A-I      intronic chr1:89281661(+) A-I      intronic chr1:89283212(+) A-I      intronic chr1:89257045(+) A-I      intronic chr1:89288823(+) A-I      intronic chr1:89254308(+) A-I      intronic chr1:89268188(+) A-I      intronic chr1:89254328(+) A-I      intronic chr1:89278222(+) A-I      intronic chr1:89242316(+) A-I      intronic chr1:89288401(+) A-I      intronic chr1:89289237(+) A-I      intronic chr1:89286003(+) A-I      intronic chr1:89278408(+) A-I      intronic chr1:89282096(+) A-I      intronic chr1:89176578(+) A-I      intronic chr1:89160588(+) A-I      intronic chr1:89240915(+) A-I      intronic chr1:89278227(+) A-I      intronic chr1:89189209(+) A-I      intronic chr1:89289613(+) A-I      intronic chr1:89268182(+) A-I      intronic chr1:89283336(+) A-I      intronic chr1:89242145(+) A-I      intronic chr1:89208752(+) A-I      intronic chr1:89189270(+) A-I      intronic chr1:89288753(+) A-I      intronic chr1:89275518(+) A-I      intronic chr1:89204902(+) A-I      intronic chr1:89230751(+) A-I      intronic chr1:89239063(+) A-I      intronic chr1:89288630(+) A-I      intronic chr1:89281613(+) A-I      intronic chr1:89167852(+) A-I      intronic chr1:89288744(+) A-I      intronic chr1:89229062(+) A-I      intronic chr1:89214557(+) A-I      intronic chr1:89167916(+) A-I      intronic chr1:89204946(+) A-I      intronic chr1:89230880(+) A-I      intronic chr1:89281703(+) A-I      intronic chr1:89176758(+) A-I      intronic chr1:89282164(+) A-I      intronic chr1:89284211(+) A-I      intronic chr1:89283284(+) A-I      intronic chr1:89223575(+) A-I      intronic chr1:89281667(+) A-I      intronic chr1:89254255(+) A-I      intronic chr1:89275519(+) A-I      intronic chr1:89288533(+) A-I      intronic chr1:89286204(+) A-I      intronic chr1:89239160(+) A-I      intronic chr1:89252602(+) A-I      intronic chr1:89240461(+) A-I      intronic chr1:89281565(+) A-I      intronic chr1:89288319(+) A-I      intronic chr1:89288361(+) A-I      intronic chr1:89275520(+) A-I      intronic chr1:89288877(+) A-I      intronic chr1:89286064(+) A-I      intronic chr1:89281985(+) A-I      intronic chr1:89281743(+) A-I      intronic chr1:89176840(+) A-I      intronic chr1:89275404(+) A-I      intronic chr1:89274194(+) A-I      intronic chr1:89268010(+) A-I      intronic chr1:89240553(+) A-I      intronic chr1:89176715(+) A-I      intronic chr1:89278161(+) A-I      intronic chr1:89275576(+) A-I      intronic chr1:89230631(+) A-I      intronic chr1:89282030(+) A-I      intronic chr1:89288342(+) A-I      intronic chr1:89286161(+) A-I      intronic chr1:89230795(+) A-I      intronic chr1:89272817(+) A-I      intronic chr1:89239045(+) A-I      intronic chr1:89223564(+) A-I      intronic chr1:89176768(+) A-I      intronic chr1:89288647(+) A-I      intronic chr1:89252686(+) A-I      intronic chr1:89289541(+) A-I      intronic chr1:89176716(+) A-I      intronic chr1:89288815(+) A-I      intronic chr1:89282069(+) A-I      intronic chr1:89268194(+) A-I      intronic chr1:89268032(+) A-I      intronic chr1:89282059(+) A-I      intronic chr1:89288796(+) A-I      intronic chr1:89275507(+) A-I      intronic chr1:89188363(+) A-I      intronic chr1:89239341(+) A-I      intronic chr1:89275384(+) A-I      intronic chr1:89288445(+) A-I      intronic chr1:89275405(+) A-I      intronic chr1:89257061(+) A-I      intronic chr1:89208709(+) A-I      intronic chr1:89281712(+) A-I      intronic chr1:89283149(+) A-I      intronic chr1:89221752(+) A-I      intronic chr1:89210266(+) A-I      intronic chr1:89223568(+) A-I      intronic chr1:89267064(+) A-I      intronic chr1:89188224(+) A-I      intronic chr1:89289439(+) A-I      intronic chr1:89289510(+) A-I      intronic chr1:89278167(+) A-I      intronic chr1:89278168(+) A-I      intronic chr1:89289240(+) A-I      intronic chr1:89266812(+) A-I      intronic chr1:89288307(+) A-I      intronic chr1:89288536(+) A-I      intronic chr1:89289123(+) A-I      intronic chr1:89167004(+) A-I      intronic chr1:89160653(+) A-I      intronic chr1:89275568(+) A-I      intronic chr1:89278298(+) A-I      intronic chr1:89288362(+) A-I      intronic chr1:89278226(+) A-I      intronic chr1:89254185(+) A-I      intronic chr1:89230700(+) A-I      intronic chr1:89278221(+) A-I      intronic chr1:89240492(+) A-I      intronic chr1:89293630(+) A-I      intronic chr1:89202219(+) A-I      intronic chr1:89255098(+) A-I      intronic chr1:89288427(+) A-I      intronic chr1:89230797(+) A-I      intronic chr1:89244514(+) A-I      intronic chr1:89289156(+) A-I      intronic chr1:89223578(+) A-I      intronic chr1:89289581(+) A-I      intronic chr1:89268231(+) A-I      intronic

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	PKN2	chr1:89176758(+) A-I intron -    22327324 chr1:89160992(+) A-I intron -    21960545 chr1:89176578(+) A-G intron -    22484847 chr1:89176715(+) A-G intron -    22484847 chr1:89176716(+) A-G intron -    22484847 chr1:89176768(+) A-G intron -    22484847 chr1:89176840(+) A-G intron -    22484847 chr1:89160588(+) A-G intron -    22484847 chr1:89160653(+) A-G intron -    22484847 chr1:89166863(+) A-G intron -    22484847 chr1:89167825(+) A-G intron -    22484847 chr1:89167852(+) A-G intron -    22484847 chr1:89167916(+) A-G intron -    22484847 chr1:89188224(+) A-G intron -    22484847 chr1:89188359(+) A-G intron -    22484847 chr1:89188363(+) A-G intron -    22484847 chr1:89189208(+) A-G intron -    22484847 chr1:89189286(+) A-G intron -    22484847 chr1:89202219(+) A-G intron -    22484847 chr1:89202245(+) A-G intron -    22484847 chr1:89208752(+) A-G intron -    22484847 chr1:89221752(+) A-G intron -    22484847 chr1:89229052(+) A-G intron -    22484847 chr1:89229062(+) A-G intron -    22484847 chr1:89239324(+) A-G intron -    22484847 chr1:89239341(+) A-G intron -    22484847 chr1:89240461(+) A-G intron -    22484847 chr1:89240492(+) A-G intron -    22484847 chr1:89242261(+) A-G intron -    22484847 chr1:89244514(+) A-G intron -    22484847 chr1:89254185(+) A-G intron -    22484847 chr1:89254255(+) A-G intron -    22484847 chr1:89254308(+) A-G intron -    22484847 chr1:89254328(+) A-G intron -    22484847 chr1:89254375(+) A-G intron -    22484847 chr1:89255098(+) A-G intron -    22484847 chr1:89255164(+) A-G intron -    22484847 chr1:89255242(+) C-A intron -    22484847 chr1:89255333(+) A-G intron -    22484847 chr1:89268032(+) A-G intron -    22484847 chr1:89268109(+) A-G intron -    22484847 chr1:89268182(+) A-G intron -    22484847 chr1:89268188(+) A-G intron -    22484847 chr1:89275363(+) A-G intron -    22484847 chr1:89275374(+) A-G intron -    22484847 chr1:89275404(+) A-G intron -    22484847 chr1:89275405(+) A-G intron -    22484847 chr1:89275507(+) A-G intron -    22484847 chr1:89275518(+) A-G intron -    22484847 chr1:89275519(+) A-G intron -    22484847 chr1:89275520(+) A-G intron -    22484847 chr1:89275568(+) A-G intron -    22484847 chr1:89278161(+) A-G intron -    22484847 chr1:89278168(+) A-G intron -    22484847 chr1:89278221(+) A-G intron -    22484847 chr1:89278222(+) A-G intron -    22484847 chr1:89278226(+) A-G intron -    22484847 chr1:89278227(+) A-G intron -    22484847 chr1:89281607(+) A-G intron -    22484847 chr1:89281622(+) A-G intron -    22484847 chr1:89281667(+) A-G intron -    22484847 chr1:89281676(+) A-G intron -    22484847 chr1:89281702(+) A-G intron -    22484847 chr1:89281703(+) A-G intron -    22484847 chr1:89281712(+) A-G intron -    22484847 chr1:89281985(+) A-G intron -    22484847 chr1:89282029(+) A-G intron -    22484847 chr1:89282030(+) A-G intron -    22484847 chr1:89282032(+) A-G intron -    22484847 chr1:89282059(+) A-G intron -    22484847 chr1:89282069(+) A-G intron -    22484847 chr1:89282096(+) A-G intron -    22484847 chr1:89283149(+) A-G intron -    22484847 chr1:89288319(+) A-G intron -    22484847 chr1:89288342(+) A-G intron -    22484847 chr1:89288361(+) A-G intron -    22484847 chr1:89288362(+) A-G intron -    22484847 chr1:89288379(+) A-G intron -    22484847 chr1:89288462(+) A-G intron -    22484847 chr1:89288630(+) A-G intron -    22484847 chr1:89288646(+) A-G intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	PKN2	chr1:89150025-89150026(+) m6A 5'UTR       24284625//24981863//22608085 chr1:89150092-89150093(+) m6A 5'UTR       24284625//24209618//24981863//22608085 chr1:89150304-89150305(+) m6A 5'UTR//CDS       24284625//24981863//22608085 chr1:89206725-89206726(+) m6A 5'UTR//CDS       24284625//24981863//27773535//22608085 chr1:89206798-89206799(+) m6A 5'UTR//CDS       24284625//24981863//27773535//22608085 chr1:89206869-89206870(+) m6A 5'UTR//CDS       24981863//27773535//22608085 chr1:89206906-89206907(+) m6A 5'UTR//CDS       24981863//27773535//22608085 chr1:89225915-89225916(+) m6A 5'UTR//CDS       24981863//27773535//22608085 chr1:89225983-89225984(+) m6A 5'UTR//CDS       24981863 chr1:89225998-89225999(+) m6A 5'UTR//CDS       24981863 chr1:89226010-89226011(+) m6A 5'UTR//CDS       24981863 chr1:89294237-89294238(+) m6A CDS       24981863 chr1:89298654-89298655(+) m6A exon//intron       24981863 chr1:89298664-89298665(+) m6A exon//intron       24981863 chr1:89298952-89298953(+) m6A CDS//exon       24284625//24209618//24981863//27773535//22575960//22608085 chr1:89299061-89299062(+) m6A CDS//exon       24284625//24209618//25456834//24981863//26404942//27773535//22575960//22608085 chr1:89299139-89299140(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//27773535//22575960//22608085 chr1:89299149-89299150(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//27773535//22575960//22608085 chr1:89299170-89299171(+) m6A 3'UTR       24284625//24209618//25456834//24981863//26404942//27773535//22575960//22608085 chr1:89299253-89299254(+) m6A 3'UTR       24284625//24209618//24981863//27773535//22575960//22608085 chr1:89299342-89299343(+) m6A 3'UTR       24284625//24981863//27773535//22608085 chr1:89299370-89299371(+) m6A 3'UTR       24284625//24981863//27773535//22608085 chr1:89299439-89299440(+) m6A 3'UTR       22608085 chr1:89299610-89299611(+) m6A 3'UTR       22608085 chr1:89299630-89299631(+) m6A 3'UTR       22608085 chr1:89299677-89299678(+) m6A 3'UTR       22608085 chr1:89299730-89299731(+) m6A 3'UTR       22608085 chr1:89299797-89299798(+) m6A 3'UTR       24981863//22608085 chr1:89299818-89299819(+) m6A 3'UTR       24981863//22608085 chr1:89299829-89299830(+) m6A 3'UTR       24981863//22608085 chr1:89299870-89299871(+) m6A 3'UTR       24981863//22608085 chr1:89299886-89299887(+) m6A 3'UTR       24981863//22608085 chr1:89299964-89299965(+) m6A 3'UTR       22608085 chr1:89300163-89300164(+) Y 3'UTR       25219674 chr1:89301109-89301110(+) m6A 3'UTR       27773535

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	PKN2	Chromatin K562 cells     - Cytosol HCC cell line (HepG2)     - Exosome Blood     - Membrane HCC cell line (HepG2)     - Nucleolus K562 cells     - Nucleoplasm K562 cells     - Nucleus Colon cancer cells     24393600 Nucleus HCC cell line (HepG2)|K562 cells     -

Interaction Network (The top 100 interactions):

Interactor1: kshv-miR-K12-10b
Interactor2: PKN2

Evidence Support:

Strong-Evidence	3'UTR indicator assay
Weak-Evidence	PAR-CLIP
Prediction-Evidence	PARalyzer
Support Database	ViRBase

References:

PMID	23170179	Target region	3'UTR
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	BC-1 cells//BC-3 cells	Interactor2 expression	Downregulation
Description	Here,we explore the similarities and differences in the functions of miRNA targets from the limited number of experimentally validated miRNA targets and the larger number of targets predicted by recent reports using high-throughput sequencing of RNA isolated by crosslinking immunoprecipitation(HITS-CLIP and PAR-CLIP) (Table 2).Table 2 Validated targets from high-throughput studies. Shown are cellular targets identified by photoactivatable ribonucleoside enhanced crosslinking and immunoprecipitation(PAR-CLIP), High-throughput sequencing of RNA isolated by crosslinking immunoprecipitation and microarray(RIP-Chip)techniques. Validation of these targets was done using luciferase reporter assays.