Basic Information:

  VIRBase ID:  

VHID00424510

  Virus:  

Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)

  Host:  

Homo sapiens

  Confidence Score:  

0.9978

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      kshv-miR-K12-10a-3p:  MIMAT0002179          TNFRSF12A:  NM_016639     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	kshv-miR-K12-10a-3p	TNFRSF12A
miRBase Accession/Entrez ID	MIMAT0002179	51330
Organism	Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	CD266//FN14//TWEAKR

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	TNFRSF12A	chr16:3072242(+) A-I      3'UTR chr16:3072245(+) A-I      3'UTR chr16:3072286(+) A-I      3'UTR chr16:3072284(+) A-I      3'UTR chr16:3072264(+) A-I      3'UTR chr16:3072248(+) A-I      3'UTR

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	TNFRSF12A	chr16:3070372-3070373(+) m6A 5'UTR//intron       24284625//24981863 chr16:3070501-3070502(+) m6A CDS//exon//intron       24284625//24981863//22575960 chr16:3070517-3070518(+) m6A CDS//exon//intron       24284625//22575960 chr16:3070543-3070544(+) m6A CDS//exon//intron       24284625//22575960 chr16:3070559-3070560(+) m6A CDS//exon//intron       24284625 chr16:3070569-3070570(+) m6A CDS//exon//intron       24284625 chr16:3070578-3070579(+) m6A CDS//exon//intron       24284625 chr16:3070617-3070618(+) m6A CDS//exon//intron       25456834 chr16:3071160-3071161(+) m6A CDS//exon//intron       22575960 chr16:3071254-3071255(+) m6A 5'UTR//CDS//exon//intron       24284625//22575960 chr16:3071260-3071261(+) m6A 5'UTR//CDS//exon//intron       24284625//22575960 chr16:3071272-3071273(+) m6A CDS//exon//intron       24284625//22575960 chr16:3071785-3071786(+) m6A 3'UTR//CDS//exon       24284625//24209618//22575960 chr16:3071990-3071991(+) m6A 3'UTR//exon       24284625//24209618//25456834//24981863//26404942//22575960//22608085 chr16:3072030-3072031(+) m6A 3'UTR//exon       24284625//24209618//25456834//24981863//26404942//22575960//22608085 chr16:3072063-3072064(+) m6A 3'UTR//exon       24284625//24209618//25456834//24981863//26404942//27773535//22575960//22608085 chr16:3072084-3072085(+) m6A 3'UTR//exon       24284625//24209618//25456834//24981863//26404942//27773535//22575960//22608085 chr16:3072160-3072161(+) m6A 3'UTR//exon       24284625//24209618//24981863//26404942//27773535//22575960 chr16:3072231-3072232(+) m6A 3'UTR//exon       24284625//26404942//27773535//22575960 chr16:3072287-3072288(+) m6A 3'UTR//exon       27773535

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	TNFRSF12A	Endoplasmic reticulum Human myelogenous leukemia cell line (K-562)     21613539 Exosome Blood     - Nucleoplasm K562 cells     - Ribosome Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: kshv-miR-K12-10a-3p
Interactor2: TNFRSF12A

Evidence Support:

Strong-Evidence	3'UTR indicator assay//3'UTR reporter assay//Luciferase reporter assay//qRT-PCR//Western blot
Weak-Evidence	Microarray//PAR-CLIP
Prediction-Evidence	cRep//miRanda//PARalyzer//RNAHybrid//Targetscan
Support Database	RepTar//ViRBase//VIRmiRNA//VmiReg

References:

[1]PMID	23170179	Target region	3'UTR
Source	RepTar//ViRBase	Interactor1 expression	None
Tissue or cell line	293 cells//SLK+K cells	Interactor2 expression	Downregulation
Description	Each method has inherent advantages and limitations, but the combination of these approaches have been successful in identifying many miRNA targets (Table 1). Table 1 List of experimentally validated cellular targets of KSHV- and EBV- encoded miRNAs. The normal cellular functions of these proteins and the effect of their knockdown during viral infection are also listed. The targets that are underlined are repressed by both KSHV- and EBV-encoded miRNAs.

[2]PMID	21152115	Target region	3'UTR
Source	RepTar//ViRBase	Interactor1 expression	None
Tissue or cell line	293 cells//SLK+K cells	Interactor2 expression	Downregulation
Description	More studies from recent reports indicate miR-K12-5, miR-K12-9 and miR-K12-10 repression of Bcl-2-associated transcription factor 1, an apoptosis-inducing factor (238), miR-K12-3 reduction of LRRC8D (leucine rich repeat containing 8 family, member D), a leucin-rich type III transmembrane protein involved in proliferation and activation of lymphocytes, NHP2L1 (non-histone chromosome protein 2-like 1) (239), and a transcription factor NFIB (240), miR-K12-1 inhibition of p21 241 and NFkappaB inhibitor IkappaBalpha (242), miR-K12-4-3p suppression of GEMIN8 (239), miR-K12-10a reduction of TWEAKR (243), miR-K12-1, -6, and -11 decrease of MAF (musculoaponeurotic fibrosarcoma oncogene homolog) (244), K12-4-5p targeting retinoblastoma (Rb)-like protein 2 (Rbl2) and miR-K12-5 and -9*suppressing ORF50 mRNA (245,246).

[3]PMID	21277611	Target region	3'UTR
Source	RepTar//ViRBase	Interactor1 expression	None
Tissue or cell line	293 cells//SLK+K cells	Interactor2 expression	Downregulation
Description	Furthermore, miR-K10a-mediated downregulation of TWEAKR prevents TWEAK-induced caspase activation, and alleviating TWEAKR repression in infected cells via a specific miR-K10a inhibitor reverses this effect.

[4]PMID	21616184	Target region	3'UTR
Source	RepTar//ViRBase//VIRmiRNA//VmiReg	Interactor1 expression	None
Tissue or cell line	293 cells//SLK+K cells	Interactor2 expression	Downregulation
Description	Previous literature suggested TNFRSF12A plays a role in apoptosis and the proinflammatory response. Multiple assays revealed miR-K12-10a repression of TNFRSF12A protects cells from cytokine-induced apoptosis and suppresses a proinflammatory cytokine response induced by TNFSF12/TWEAK.

[5]PMID	22100165	Target region	3'UTR
Source	RepTar//ViRBase//VIRmiRNA//VmiReg	Interactor1 expression	None
Tissue or cell line	BC-3 cells	Interactor2 expression	None
Description	Our dataset also confirmed 12 out of 29 validated KSHV miRNA targets with expression in our dataset(>40%). Confirmed interactions include those with BACH1, FOS, CDKN1A(p21), TNFRSF12A(TWEAKR), RAD21 and RBL2 mRNAs, whose regulation had previously been validated at the level of protein expression. A list summarizing the recovery of previously published targets of the KSHV and EBV miRNAs is presented in Table S9. NIHMS333942-supplement-01 Table S9 Recovery of previously published targets for KSHV or EBV miRNAs.

[6]PMID	20844036	Target region	3'UTR
Source	RepTar//ViRBase//VIRmiRNA//VmiReg	Interactor1 expression	None
Tissue or cell line	293 cells//SLK+K cells	Interactor2 expression	Downregulation
Description	Tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) receptor (TWEAKR) was among the most consistently and robustly downregulated genes in the presence of KSHV miR-K12-10a (miR-K10a).