Detail Information
Interactor1 | Interactor2 | |
---|---|---|
Symbol | kshv-miR-K12-10a-3p | BCLAF1 |
miRBase Accession/Entrez ID |
MIMAT0002179 | 9774 |
Organism | Human gammaherpesvirus 8 (Kaposi's sarcoma herpesvirus, KSHV) | Homo sapiens |
Category | miRNA | mRNA |
Alias | - | bK211L9.1//BTF |
Resource | Symbol | Editing Position | Change | Genetic Region | ||||||
---|---|---|---|---|---|---|---|---|---|---|
RADAR | BCLAF1 |
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Resource | Symbol | ModificationPosition | Type | Genomic Context | PMID | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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RMBase | BCLAF1 |
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Resource | Symbol | Subcellular Localization | Tissue or Cell Line | PMID | |||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
RNALocate | BCLAF1 |
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Interactor1: kshv-miR-K12-10a-3p | Interactor2: BCLAF1 |
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Strong-Evidence | Luciferase reporter assay//Western blot | |
---|---|---|
Weak-Evidence | Microarray//PAR-CLIP | |
Prediction-Evidence | miRanda//PARalyzer//Targetscan | |
Support Database | ViRBase//VmiReg |
[1]PMID | 21277611 | Target region | 3'UTR |
---|---|---|---|
Source | ViRBase | Interactor1 expression | None |
Tissue or cell line | 293 cells//BCBL-1 cells//BCBL-3 cells//BJAB cells//HUVECs | Interactor2 expression | Downregulation |
Description | An overview of known targets of cellular or viral targets is given in tables 2 and 3.Table 2 Cellular Targets of viral miRNAs.Table 3 Viral Targets of viral miRNAs. |
[2]PMID | 19098914 | Target region | 3'UTR |
---|---|---|---|
Source | ViRBase//VmiReg | Interactor1 expression | None |
Tissue or cell line | 293 cells//BCBL-1 cells//BCBL-3 cells//BJAB cells//HUVECs | Interactor2 expression | Downregulation |
Description | Fig.3a shows that, in addition to miR-K5, several other KSHV miRs down-regulated this protein reproducibly-notably, miRs K9, K10a and K10b. Each of these miRNAs was tested for the ability to downregulate the LUC-BCLAF1 3'UTR reporter construct (Fig.3b). |
[3]PMID | 21616184 | Target region | 3'UTR |
---|---|---|---|
Source | ViRBase//VmiReg | Interactor1 expression | None |
Tissue or cell line | 293 cells//BCBL-1 cells//BCBL-3 cells//BJAB cells//HUVECs | Interactor2 expression | Downregulation |
Description | Currently, the validated targets of KSHV miRNAs (Table 1) represent a collection of genes identified by unbiased methods or chosen by previously identified functions.Table 1 KSHVmiRNA validated targets. All targets have been validated using ectopic expression of miRNAs to demonstrate that a luciferase reporter or ectopic reporter are repressed. Additional validations include(1) exogenous reporters have been mutated to disrupt targeting by a ectopic miRNA,(2) ectopic miRNA suppresses endogenous target mRNA and/or protein levels,(3) de novo infection with KSHV represses endogenous target expression,(4) miRNA inhibitors and/or mutant virus display derepression of endogenous target mRNA and/or protein levels, and(5) repression of miRNA target is observed in KSHV clinical samples. |
[4]PMID | 22100165 | Target region | 3'UTR |
---|---|---|---|
Source | ViRBase//VmiReg | Interactor1 expression | None |
Tissue or cell line | PEL cells | Interactor2 expression | None |
Description | Our dataset also confirmed 12 out of 29 validated KSHV miRNA targets with expression in our dataset(>40%). Confirmed interactions include those with BACH1, FOS, CDKN1A(p21), TNFRSF12A(TWEAKR), RAD21 and RBL2 mRNAs, whose regulation had previously been validated at the level of protein expression. A list summarizing the recovery of previously published targets of the KSHV and EBV miRNAs is presented in Table S9. NIHMS333942-supplement-01 Table S9 Recovery of previously published targets for KSHV or EBV miRNAs. |