Basic Information:

  VIRBase ID:  

VHID00397299

  Virus:  

Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)

  Host:  

Homo sapiens

  Confidence Score:  

0.6002

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      ebv-miR-BART9-5p:  MIMAT0004816          PRKAR1A:  NM_212471     

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Interactor Information:

	Interactor1	Interactor2
Symbol	ebv-miR-BART9-5p	PRKAR1A
miRBase Accession/Entrez ID	MIMAT0004816	5573
Organism	Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	ACRDYS1//ADOHR//CAR//CNC//CNC1//PKR1//PPNAD1//PRKAR1//TSE1

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	PRKAR1A	chr17:66508177-66508178(+) m6A 5'UTR       24284625//24209618//25456834//24981863//27371828//22608085 chr17:66508249-66508250(+) m6A 5'UTR       24284625//24209618//25456834//24981863//27371828//22608085 chr17:66508265-66508266(+) m6A 5'UTR       24284625//24209618//25456834//24981863//27371828//22608085 chr17:66508279-66508280(+) m6A 5'UTR       24284625//24209618//25456834//24981863//27371828//22608085 chr17:66508637-66508638(+) Y 5'UTR//exon//intron       26075521 chr17:66509051-66509052(+) m6A 5'UTR//intron       - chr17:66511674-66511675(+) m6A CDS//exon       27773535 chr17:66518907-66518908(+) m6A CDS//exon       27773535 chr17:66518945-66518946(+) m6A CDS//exon       27773535 chr17:66520202-66520203(+) m6A 3'UTR//CDS//exon       27773535 chr17:66520265-66520266(+) m6A exon//intron       24981863//27773535 chr17:66521950-66521951(+) m6A 3'UTR//CDS       24981863//26404942//27773535 chr17:66521969-66521970(+) m6A 3'UTR//CDS       24981863//26404942//27773535 chr17:66521999-66522000(+) m6A 3'UTR//CDS       24981863//26404942//27773535 chr17:66522030-66522031(+) m6A 3'UTR//CDS       24981863//26404942//27773535 chr17:66524853-66524854(+) m6A 3'UTR//intron       27773535 chr17:66525019-66525020(+) m6A 3'UTR//CDS//exon       27773535 chr17:66525056-66525057(+) m6A 3'UTR//CDS//exon       27773535 chr17:66525101-66525102(+) m6A 3'UTR//CDS//exon       27773535 chr17:66526982-66526983(+) m6A 3'UTR//intron       26404942 chr17:66527034-66527035(+) m6A 3'UTR//intron       26404942 chr17:66527618-66527619(+) m6A 3'UTR//intron       26404942 chr17:66528407-66528408(+) Y 3'UTR//intron       25219674 chr17:66528783-66528784(+) m6A 3'UTR//intron       27773535 chr17:66541435-66541436(+) m6A intron       27773535 chr17:66541450-66541451(+) m6A intron       27773535 chr17:66541484-66541485(+) m6A intron       27773535

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	PRKAR1A	Chromatin K562 cells     - Cytosol Human myelogenous leukemia cell line (K-562)     21613539 Exosome Blood     - Nucleolus K562 cells     - Nucleus HCC cell line (HepG2)|HeLa-S3 cells|K562 cells     - Ribosome Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: ebv-miR-BART9-5p
Interactor2: PRKAR1A

Evidence Support:

Weak-Evidence	PAR-CLIP
Prediction-Evidence	miRanda//PARalyzer//Targetscan
Support Database	ViRBase//VIRmiRNA//VmiReg

References:

PMID	22100165	Target region	3'UTR
Source	ViRBase//VIRmiRNA//VmiReg	Interactor1 expression	None
Tissue or cell line	BC-1 cells	Interactor2 expression	Downregulation
Description	Using PAR-CLIP, a technology which allows the direct and transcriptome-wide identification of miRNA targets, we delineate the target sites for all viral and cellular miRNAs expressed in PEL cell lines. The resulting data set revealed that KSHV miRNAs directly target more than 2000 cellular mRNAs, including many involved in pathways relevant to KSHV pathogenesis. Moreover, 58% of these mRNAs are also targeted by EBV miRNAs, via distinct binding sites.Clusters with seed matches to expressed miRNAs were considered candidate target sites. Of the clusters mapping to human 3'UTRs, 69% (BC-1) and 70% (BC-3) had seed matches to expressed miRNAs (Tables S6).Table S6A BC-1 derived 3'UTR clusters with seed matches to expressed miRNAs.