Basic Information:

  VIRBase ID:  

VHID00371275

  Virus:  

Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)

  Host:  

Homo sapiens

  Confidence Score:  

0.8808

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      Predicted binding sites in PRIdictor:   BCL6:  NP_001128210     

Binding sites in PDB
PDB	1R28//1R29//1R2B//2EN2//2EOS//2LCE//2YRM//3BIM//3E4U//3LBZ//4CP3//4U2M

Interactor Information:

	Interactor1	Interactor2
Symbol	ebv-miR-BART17-5p	BCL6
miRBase Accession/Entrez ID	MIMAT0003715	604
Organism	Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)	Homo sapiens
Category	miRNA	protein
Alias	-	BCL5//BCL6A//LAZ3//ZBTB27//ZNF51

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	BCL6	chr3:187442376-187442377(-) m6A exon//intron       27773535 chr3:187442435-187442436(-) m6A exon//intron       27773535 chr3:187443309-187443310(-) m6A 3'UTR//CDS//exon       27773535 chr3:187443354-187443355(-) m6A 3'UTR//CDS//exon       27773535 chr3:187443409-187443410(-) m6A 3'UTR//CDS//exon       27773535 chr3:187444530-187444531(-) m6A 3'UTR//CDS//intron       27773535 chr3:187444594-187444595(-) m6A 3'UTR//CDS//intron       27773535 chr3:187444608-187444609(-) m6A 3'UTR//CDS//intron       27773535 chr3:187446177-187446178(-) m6A 3'UTR//CDS       27773535 chr3:187446882-187446883(-) m6A 3'UTR//CDS       24284625//27773535//27371828 chr3:187446927-187446928(-) m6A 3'UTR//CDS       24284625//27773535//27371828 chr3:187447019-187447020(-) m6A 3'UTR//CDS       24284625//24981863//27773535//27371828 chr3:187447221-187447222(-) m6A 3'UTR//CDS       24284625//24209618//24981863//27773535 chr3:187447277-187447278(-) m6A 3'UTR//CDS       24284625//24209618//24981863//27773535 chr3:187447284-187447285(-) m1A 3'UTR//CDS       26863196 chr3:187447346-187447347(-) m6A 3'UTR//CDS       24284625//24981863//27773535 chr3:187447402-187447403(-) m6A 3'UTR//CDS       24284625//24981863//27773535 chr3:187447698-187447699(-) m6A 3'UTR//CDS       27773535 chr3:187447734-187447735(-) m6A 3'UTR//CDS       27773535 chr3:187451485-187451486(-) m6A 5'UTR//exon       27371828 chr3:187463203-187463204(-) m6A 5'UTR//exon       - chr3:187463252-187463253(-) m6A 5'UTR//exon       -

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	BCL6	Cytosol Human myelogenous leukemia cell line (K-562)     21613539 Cytosol HCC cell line (HepG2)|K562 cells     - Exosome Blood     -

Interaction Network (The top 100 interactions):

Interactor1: ebv-miR-BART17-5p
Interactor2: BCL6

Evidence Support:

Strong-Evidence	Luciferase reporter assay//Western blot
Support Database	VmiReg

References:

PMID	21788950	Target region	3'UTR
Source	VmiReg	Interactor1 expression	None
Tissue or cell line	DB cells//HEK293T cells//SU-DHL-4 cells//SU-DHL-6 cells	Interactor2 expression	None
Description	With this approach, we noted a significant reduction in luciferase signal for three of the four viral miRNAs assayed (Figure 2a) when compared with either the reporter transfected with a miRNA mimic negative control sequence or the vector without the 3'UTR of BCL6. Ebv-miR-BART3, ebv-miR-BART9 and ebvmiR- BART17-5p reduced the luciferase signal by at least 60%.