Basic Information:

  VIRBase ID:  

VHID00347238

  Virus:  

Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)

  Host:  

Homo sapiens

  Confidence Score:  

0.6787

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      ebv-miR-BART13-3p:  MIMAT0003424          EIF5:  NM_183004     

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Interactor Information:

	Interactor1	Interactor2
Symbol	ebv-miR-BART13-3p	EIF5
miRBase Accession/Entrez ID	MIMAT0003424	1983
Organism	Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	EIF-5//EIF-5A

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	EIF5	chr14:103803797(+) A-I      intronic chr14:103802797(+) A-I      intronic chr14:103806257(+) A-I      intronic chr14:103803760(+) A-I      intronic

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	EIF5	chr14:103800463-103800464(+) m6A 5'UTR//intron       25456834//24981863 chr14:103800586-103800587(+) m6A 5'UTR//exon//intron       25456834//24981863//27371828//27773536 chr14:103800755-103800756(+) m6A 5'UTR//exon//intron       25456834//24981863//27371828//27773536//22575960 chr14:103800773-103800774(+) m6A 5'UTR//exon//intron       24981863//27371828//22575960 chr14:103800848-103800849(+) m6A 5'UTR//exon//intron       22575960 chr14:103800854-103800855(+) m6A 5'UTR//exon//intron       22575960 chr14:103803051-103803052(+) m6A CDS//exon       27773535 chr14:103803118-103803119(+) m6A CDS//exon       27773535 chr14:103803177-103803178(+) m6A CDS//exon       27773535 chr14:103803290-103803291(+) m6A exon//intron       27773535 chr14:103803297-103803298(+) m6A exon//intron       27773535 chr14:103803473-103803474(+) m6A CDS//exon       27773535 chr14:103803532-103803533(+) m6A CDS//exon       24981863//27773535 chr14:103803555-103803556(+) m6A CDS//exon       24981863//27773535 chr14:103803582-103803583(+) m6A exon//intron       24981863//27773535 chr14:103803620-103803621(+) m6A exon//intron       24981863//27773535 chr14:103803660-103803661(+) m6A exon//intron       24981863//27773535 chr14:103804233-103804234(+) Cm intron       - chr14:103804708-103804709(+) m6A CDS//exon       24981863//27773535//22608085 chr14:103804723-103804724(+) m6A CDS//exon       24981863//27773535//22608085 chr14:103804755-103804756(+) m6A CDS//exon       24981863//27773535//22608085 chr14:103805188-103805189(+) m6A CDS//exon       27773535 chr14:103805570-103805571(+) m6A CDS//exon       27773535 chr14:103805636-103805637(+) m6A CDS//exon       27773535 chr14:103806099-103806100(+) m6A CDS//exon       27773535 chr14:103806742-103806743(+) m6A exon//intron       27773535 chr14:103806789-103806790(+) m6A CDS//exon       24981863//26404942//27773535 chr14:103806819-103806820(+) m6A CDS//exon       24981863//26404942//27773535 chr14:103806890-103806891(+) m6A CDS//exon       26404942//27773535//22575960 chr14:103807338-103807339(+) m6A CDS//exon       26404942//27773535//22575960 chr14:103807351-103807352(+) m6A CDS//exon       26404942//27773535//22575960 chr14:103808560-103808561(+) m6A 3'UTR//exon       - chr14:103808770-103808771(+) m6A 3'UTR       27773535 chr14:103809298-103809299(+) m6A 3'UTR       27773535 chr14:103809318-103809319(+) m6A 3'UTR       27773535 chr14:103809339-103809340(+) m6A 3'UTR       27773535 chr14:103809362-103809363(+) m6A 3'UTR       27773535 chr14:103809395-103809396(+) m6A 3'UTR       27773535 chr14:103811178-103811179(+) m6A 3'UTR       24981863//27773535

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	EIF5	Chromatin K562 cells     - Cytosol Human myelogenous leukemia cell line (K-562)     21613539 Cytosol HCC cell line (HepG2)|K562 cells     - Exosome Blood     - Membrane HCC cell line (HepG2)     - Nucleolus K562 cells     - Nucleus HeLa-S3 cells     - Ribosome HEK-293 cells     22199352 Ribosome Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: ebv-miR-BART13-3p
Interactor2: EIF5

Evidence Support:

Weak-Evidence	HITS-CLIP//PAR-CLIP
Prediction-Evidence	cRep//miRanda//PARalyzer//PITA//Targetscan
Support Database	RepTar//ViRBase//VIRmiRNA//VmiReg

References:

[1]PMID	22100165	Target region	3'UTR
Source	RepTar//ViRBase//VIRmiRNA//VmiReg	Interactor1 expression	None
Tissue or cell line	BC-1 cells	Interactor2 expression	Downregulation
Description	Using PAR-CLIP, a technology which allows the direct and transcriptome-wide identification of miRNA targets, we delineate the target sites for all viral and cellular miRNAs expressed in PEL cell lines. The resulting data set revealed that KSHV miRNAs directly target more than 2000 cellular mRNAs, including many involved in pathways relevant to KSHV pathogenesis. Moreover, 58% of these mRNAs are also targeted by EBV miRNAs, via distinct binding sites.Clusters with seed matches to expressed miRNAs were considered candidate target sites. Of the clusters mapping to human 3'UTRs, 69% (BC-1) and 70% (BC-3) had seed matches to expressed miRNAs (Tables S6).Table S6A BC-1 derived 3'UTR clusters with seed matches to expressed miRNAs.

[2]PMID	22473208	Target region	3'UTR
Source	RepTar//ViRBase//VIRmiRNA//VmiReg	Interactor1 expression	None
Tissue or cell line	Jijoye cells	Interactor2 expression	None
Description	Our HITS-CLIP data yield 1185 human 3'UTRs targeted by members of the miR-17~92 cluster in Jijoye cells. Comparison to the 1664 3'UTRs targeted by EBV miRNAs reveals 740 shared genes (44% of EBV targets and 62% of miR-17~92 targets; Figure 6B and Supplementary Table 7). Thus, EBV miRNAs co-target a majority of miR-17~92 regulated mRNAs, which are assigned to a variety of pathways, most notably regulating transcription, apoptosis, and the cell cycle (Figure 6C). Similarly, other abundant immunologically relevant miRNAs, 142-3p and miR-155, co-target with EBV miRNAs a large fraction of their Ago-bound 3'UTRs, representing ~60% of the targets for each of these host miRNAs (Supplementary Figure 5).