Basic Information:

  VIRBase ID:  

VHID00326665

  Virus:  

Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)

  Host:  

Homo sapiens

  Confidence Score:  

0.6002

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      ebv-miR-BART8-3p:  MIMAT0003418          SPRYD4:  NM_207344     

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Interactor Information:

	Interactor1	Interactor2
Symbol	ebv-miR-BART8-3p	SPRYD4
miRBase Accession/Entrez ID	MIMAT0003418	283377
Organism	Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	-

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	SPRYD4	chr12:56864318(+) A-I      3'UTR chr12:56864371(+) A-I      3'UTR chr12:56864209(+) A-I      3'UTR chr12:56864311(+) A-I      3'UTR chr12:56864370(+) A-I      3'UTR chr12:56864208(+) A-I      3'UTR

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	SPRYD4	chr12:56864208(+) A-G intron -    22484847 chr12:56864209(+) A-G intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	SPRYD4	chr12:56862835-56862836(+) m6A 5'UTR//CDS       24284625//24981863//22608085 chr12:56862848-56862849(+) m6A 5'UTR//CDS       24284625//24981863//22608085 chr12:56862975-56862976(+) m6A 5'UTR//CDS       24284625//24209618//25456834//24981863//22575960//22608085 chr12:56862997-56862998(+) m6A 5'UTR//CDS       24284625//24209618//25456834//24981863//22575960//22608085 chr12:56863059-56863060(+) m6A 5'UTR//CDS       24284625//24209618//25456834//24981863//22575960//22608085 chr12:56863268-56863269(+) m6A 5'UTR//CDS       24284625//25456834//24981863 chr12:56863541-56863542(+) m6A 3'UTR//5'UTR       24284625//24981863 chr12:56863656-56863657(+) m6A 3'UTR//CDS       24284625//24981863 chr12:56872891-56872892(+) m6A 3'UTR       - chr12:56872915-56872916(+) m6A 3'UTR       - chr12:56872976-56872977(+) m6A 3'UTR       - chr12:56873014-56873015(+) m6A 3'UTR       -

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	SPRYD4	Cytoplasm Nasopharyngeal carcinoma cells     20498841 Cytosol Human myelogenous leukemia cell line (K-562)     21613539 Exosome Blood     - Nucleolus K562 cells     - Nucleoplasm K562 cells     - Nucleus Nasopharyngeal carcinoma cells     20498841 Ribosome Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: ebv-miR-BART8-3p
Interactor2: SPRYD4

Evidence Support:

Weak-Evidence	PAR-CLIP
Prediction-Evidence	PARalyzer//PITA//Targetscan
Support Database	ViRBase//VIRmiRNA//VmiReg

References:

PMID	22100165	Target region	3'UTR
Source	ViRBase//VIRmiRNA//VmiReg	Interactor1 expression	None
Tissue or cell line	BC-1 cells	Interactor2 expression	Downregulation
Description	Using PAR-CLIP, a technology which allows the direct and transcriptome-wide identification of miRNA targets, we delineate the target sites for all viral and cellular miRNAs expressed in PEL cell lines. The resulting data set revealed that KSHV miRNAs directly target more than 2000 cellular mRNAs, including many involved in pathways relevant to KSHV pathogenesis. Moreover, 58% of these mRNAs are also targeted by EBV miRNAs, via distinct binding sites.Clusters with seed matches to expressed miRNAs were considered candidate target sites. Of the clusters mapping to human 3'UTRs, 69% (BC-1) and 70% (BC-3) had seed matches to expressed miRNAs (Tables S6).Table S6A BC-1 derived 3'UTR clusters with seed matches to expressed miRNAs.