Basic Information:

  VIRBase ID:  

VHID00307378

  Virus:  

Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)

  Host:  

Homo sapiens

  Confidence Score:  

0.6002

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      ebv-miR-BART4-5p:  MIMAT0003412          MPLKIP:  NM_138701     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	ebv-miR-BART4-5p	MPLKIP
miRBase Accession/Entrez ID	MIMAT0003412	136647
Organism	Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	ABHS//C7orf11//ORF20//TTD4

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	MPLKIP	chr7:40165708-40165709(-) m6A 3'UTR       27773535 chr7:40165833-40165834(-) m6A 3'UTR       27773535 chr7:40165875-40165876(-) m6A 3'UTR       27773535 chr7:40172306-40172307(-) m6A 3'UTR       27773535 chr7:40172355-40172356(-) m6A 3'UTR       27773535 chr7:40172395-40172396(-) m6A 3'UTR       27773535//22608085 chr7:40172604-40172605(-) m6A 3'UTR       24284625//24209618//24981863//28052920//26404942//27773535//27371828//22575960//22608085 chr7:40172609-40172610(-) m6A 3'UTR       24284625//24209618//24981863//28052920//26404942//27773535//27371828//22575960//22608085 chr7:40172618-40172619(-) m6A 3'UTR       24284625//24209618//24981863//28052920//26404942//27773535//27371828//22575960//22608085 chr7:40172692-40172693(-) m6A CDS       24284625//24209618//24981863//28052920//26404942//27773535//27371828//22575960//22608085 chr7:40172738-40172739(-) m6A CDS       24284625//24209618//24981863//26404942//27773535//22575960//22608085 chr7:40172845-40172846(-) m6A CDS       27773536 chr7:40173838-40173839(-) m6A CDS       27773536 chr7:40174061-40174062(-) m6A CDS       24981863//22575960 chr7:40174078-40174079(-) m6A CDS       24981863 chr7:40174248-40174249(-) m6A 5'UTR       24284625//24981863//22608085

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	MPLKIP	Cytosol Human myelogenous leukemia cell line (K-562)     21613539 Exosome Blood     - Ribosome Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: ebv-miR-BART4-5p
Interactor2: MPLKIP

Evidence Support:

Weak-Evidence	PAR-CLIP
Prediction-Evidence	miRanda//PARalyzer//Targetscan
Support Database	ViRBase//VIRmiRNA//VmiReg

References:

[1]PMID	22100165	Target region	3'UTR
Source	ViRBase//VIRmiRNA//VmiReg	Interactor1 expression	None
Tissue or cell line	BC-1 cells	Interactor2 expression	Downregulation
Description	Using PAR-CLIP, a technology which allows the direct and transcriptome-wide identification of miRNA targets, we delineate the target sites for all viral and cellular miRNAs expressed in PEL cell lines. The resulting data set revealed that KSHV miRNAs directly target more than 2000 cellular mRNAs, including many involved in pathways relevant to KSHV pathogenesis. Moreover, 58% of these mRNAs are also targeted by EBV miRNAs, via distinct binding sites.Clusters with seed matches to expressed miRNAs were considered candidate target sites. Of the clusters mapping to human 3'UTRs, 69% (BC-1) and 70% (BC-3) had seed matches to expressed miRNAs (Tables S6).Table S6A BC-1 derived 3'UTR clusters with seed matches to expressed miRNAs.

[2]PMID	22291592	Target region	3'UTR
Source	ViRBase//VIRmiRNA//VmiReg	Interactor1 expression	None
Tissue or cell line	EF3D-AGO2 cells//LCL35 cells//LCL-BAC cells//LCL-BAC-D1 cells//LCL-BAC-D3 cells	Interactor2 expression	Downregulation
Description	We identified 7,827 miRNA-interaction sites in 3,492 cellular 3'UTRs. 531 of these sites contained seed matches to viral miRNAs.Table S6: High confidence miRNA-interaction sites in 3'UTRs.We identified an additional 2,972 CDS miRNA-interaction sites present in at least two libraries, 89.7% of which could be assigned to a miRNA (Table S7).