Basic Information:

  VIRBase ID:  

VHID00305883

  Virus:  

Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)

  Host:  

Homo sapiens

  Confidence Score:  

0.6040

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      ebv-miR-BART4-5p:  MIMAT0003412          MAP3K2:  NM_006609     

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Interactor Information:

	Interactor1	Interactor2
Symbol	ebv-miR-BART4-5p	MAP3K2
miRBase Accession/Entrez ID	MIMAT0003412	10746
Organism	Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	MEKK2//MEKK2B

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	MAP3K2	chr2:128113339(-) A-I      intronic chr2:128092221(-) A-I      intronic chr2:128113341(-) A-I      intronic chr2:128113340(-) A-I      intronic chr2:128113329(-) A-I      intronic chr2:128085794(-) A-I      intronic chr2:128113328(-) A-I      intronic chr2:128113327(-) A-I      intronic chr2:128092289(-) A-I      intronic chr2:128092224(-) A-I      intronic chr2:128092223(-) A-I      intronic chr2:128092181(-) A-I      intronic chr2:128092170(-) A-I      intronic chr2:128092166(-) A-I      intronic chr2:128070151(-) A-I      intronic chr2:128125954(-) A-I      intronic chr2:128090549(-) A-I      intronic chr2:128126076(-) A-I      intronic chr2:128126079(-) A-I      intronic chr2:128126086(-) A-I      intronic chr2:128126450(-) A-I      intronic chr2:128133022(-) A-I      intronic chr2:128133074(-) A-I      intronic chr2:128134468(-) A-I      intronic chr2:128134491(-) A-I      intronic chr2:128134509(-) A-I      intronic chr2:128135543(-) A-I      intronic chr2:128098633(-) A-I      intronic chr2:128092625(-) A-I      intronic chr2:128085837(-) A-I      intronic chr2:128125789(-) A-I      intronic chr2:128085729(-) A-I      intronic chr2:128098694(-) A-I      intronic chr2:128105881(-) A-I      intronic chr2:128085727(-) A-I      intronic chr2:128085702(-) A-I      intronic chr2:128085671(-) A-I      intronic chr2:128080910(-) A-I      intronic chr2:128080875(-) A-I      intronic chr2:128080308(-) A-I      intronic chr2:128098385(-) A-I      intronic chr2:128098384(-) A-I      intronic chr2:128098187(-) A-I      intronic chr2:128092616(-) A-I      intronic chr2:128133221(-) A-I      intronic chr2:128092316(-) A-I      intronic chr2:128092215(-) A-I      intronic chr2:128092198(-) A-I      intronic chr2:128098698(-) A-I      intronic chr2:128098730(-) A-I      intronic chr2:128101373(-) A-I      intronic chr2:128101447(-) A-I      intronic chr2:128102146(-) A-I      intronic chr2:128102524(-) A-I      intronic chr2:128105864(-) A-I      intronic chr2:128105878(-) A-I      intronic chr2:128105888(-) A-I      intronic chr2:128105901(-) A-I      intronic chr2:128114136(-) A-I      intronic chr2:128118807(-) A-I      intronic chr2:128121446(-) A-I      intronic chr2:128122487(-) A-I      intronic chr2:128122500(-) A-I      intronic chr2:128122501(-) A-I      intronic chr2:128125152(-) A-I      intronic chr2:128125241(-) A-I      intronic chr2:128125250(-) A-I      intronic chr2:128125672(-) A-I      intronic chr2:128125776(-) A-I      intronic chr2:128133155(-) A-I      intronic chr2:128089319(-) A-I      intronic chr2:128089284(-) A-I      intronic chr2:128087160(-) A-I      intronic chr2:128087159(-) A-I      intronic chr2:128087141(-) A-I      intronic chr2:128087112(-) A-I      intronic chr2:128125896(-) A-I      intronic chr2:128121397(-) A-I      intronic chr2:128133156(-) A-I      intronic chr2:128122596(-) A-I      intronic chr2:128121357(-) A-I      intronic chr2:128089352(-) A-I      intronic chr2:128090646(-) A-I      intronic chr2:128085696(-) A-I      intronic chr2:128092219(-) A-I      intronic chr2:128091492(-) A-I      intronic chr2:128121344(-) A-I      intronic chr2:128092586(-) A-I      intronic chr2:128134484(-) A-I      intronic chr2:128126661(-) A-I      intronic chr2:128126617(-) A-I      intronic chr2:128125247(-) A-I      intronic chr2:128126454(-) A-I      intronic chr2:128092572(-) A-I      intronic chr2:128080364(-) A-I      intronic chr2:128133082(-) A-I      intronic chr2:128090449(-) A-I      intronic chr2:128134513(-) A-I      intronic chr2:128091483(-) A-I      intronic chr2:128085694(-) A-I      intronic chr2:128080334(-) A-I      intronic chr2:128092297(-) A-I      intronic chr2:128102160(-) A-I      intronic chr2:128134568(-) A-I      intronic chr2:128092542(-) A-I      intronic chr2:128122582(-) A-I      intronic chr2:128091551(-) A-I      intronic chr2:128125641(-) A-I      intronic chr2:128121496(-) A-I      intronic chr2:128125309(-) A-I      intronic chr2:128087109(-) A-I      intronic chr2:128092099(-) A-I      intronic chr2:128091509(-) A-I      intronic chr2:128134525(-) A-I      intronic chr2:128125942(-) A-I      intronic chr2:128092523(-) A-I      intronic chr2:128125257(-) A-I      intronic chr2:128092565(-) A-I      intronic chr2:128091472(-) A-I      intronic chr2:128092515(-) A-I      intronic chr2:128092238(-) A-I      intronic chr2:128092581(-) A-I      intronic chr2:128092578(-) A-I      intronic chr2:128092635(-) A-I      intronic chr2:128092443(-) A-I      intronic chr2:128092401(-) A-I      intronic chr2:128136665(-) A-I      intronic chr2:128092540(-) A-I      intronic chr2:128092381(-) A-I      intronic chr2:128085849(-) A-I      intronic chr2:128107549(-) A-I      intronic chr2:128092637(-) A-I      intronic chr2:128125286(-) A-I      intronic chr2:128081089(-) A-I      intronic chr2:128102690(-) A-I      intronic chr2:128123859(-) A-I      intronic chr2:128101529(-) A-I      intronic chr2:128141288(-) A-I      intronic chr2:128116422(-) A-I      intronic chr2:128102163(-) A-I      intronic chr2:128092617(-) A-I      intronic chr2:128109712(-) A-I      intronic chr2:128092257(-) A-I      intronic chr2:128098048(-) A-I      intronic chr2:128109555(-) A-I      intronic chr2:128105816(-) A-I      intronic chr2:128125355(-) A-I      intronic chr2:128089375(-) A-I      intronic chr2:128125964(-) A-I      intronic chr2:128126115(-) A-I      intronic chr2:128085799(-) A-I      intronic chr2:128092458(-) A-I      intronic chr2:128134494(-) A-I      intronic chr2:128091614(-) A-I      intronic chr2:128124523(-) A-I      intronic chr2:128126952(-) A-I      intronic chr2:128090672(-) A-I      intronic chr2:128080291(-) A-I      intronic chr2:128126798(-) A-I      intronic chr2:128122453(-) A-I      intronic chr2:128102523(-) A-I      intronic chr2:128091613(-) A-I      intronic chr2:128126600(-) A-I      intronic chr2:128105950(-) A-I      intronic chr2:128123952(-) A-I      intronic chr2:128090597(-) A-I      intronic chr2:128116423(-) A-I      intronic chr2:128090571(-) A-I      intronic chr2:128092485(-) A-I      intronic chr2:128125841(-) A-I      intronic chr2:128133159(-) A-I      intronic chr2:128085829(-) A-I      intronic chr2:128126414(-) A-I      intronic chr2:128123877(-) A-I      intronic chr2:128126620(-) A-I      intronic chr2:128091662(-) A-I      intronic chr2:128126621(-) A-I      intronic chr2:128121479(-) A-I      intronic chr2:128092318(-) A-I      intronic chr2:128133097(-) A-I      intronic chr2:128089463(-) A-I      intronic chr2:128141276(-) A-I      intronic chr2:128085815(-) A-I      intronic chr2:128124620(-) A-I      intronic chr2:128085738(-) A-I      intronic chr2:128105957(-) A-I      intronic chr2:128085751(-) A-I      intronic chr2:128121400(-) A-I      intronic chr2:128105729(-) A-I      intronic chr2:128092160(-) A-I      intronic chr2:128106875(-) A-I      intronic chr2:128134618(-) A-I      intronic chr2:128085663(-) A-I      intronic chr2:128092239(-) A-I      intronic chr2:128122654(-) A-I      intronic chr2:128125312(-) A-I      intronic chr2:128132993(-) A-I      intronic chr2:128121451(-) A-I      intronic chr2:128136440(-) A-I      intronic chr2:128092263(-) A-I      intronic chr2:128141287(-) A-I      intronic chr2:128105692(-) A-I      intronic chr2:128134490(-) A-I      intronic chr2:128122592(-) A-I      intronic chr2:128105761(-) A-I      intronic chr2:128105737(-) A-I      intronic chr2:128125816(-) A-I      intronic chr2:128134631(-) A-I      intronic chr2:128126731(-) A-I      intronic chr2:128091453(-) A-I      intronic chr2:128090594(-) A-I      intronic chr2:128126964(-) A-I      intronic chr2:128091642(-) A-I      intronic chr2:128091458(-) A-I      intronic chr2:128092311(-) A-I      intronic chr2:128090447(-) A-I      intronic chr2:128085788(-) A-I      intronic chr2:128126460(-) A-I      intronic chr2:128092110(-) A-I      intronic chr2:128092202(-) A-I      intronic chr2:128121432(-) A-I      intronic chr2:128102537(-) A-I      intronic chr2:128125815(-) A-I      intronic chr2:128136501(-) A-I      intronic chr2:128092422(-) A-I      intronic chr2:128092252(-) A-I      intronic chr2:128091471(-) A-I      intronic chr2:128085845(-) A-I      intronic chr2:128141588(-) A-I      intronic

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	MAP3K2	chr2:128091642(-) A-I intron -    22327324 chr2:128092160(-) A-I intron -    22327324 chr2:128092311(-) A-I intron -    22327324 chr2:128092485(-) A-I intron -    22028664   //22327324 chr2:128092381(-) A-I intron -    22028664 chr2:128092399(-) A-I intron -    22028664 chr2:128092401(-) A-I intron -    22028664 chr2:128092422(-) A-I intron -    22028664 chr2:128092443(-) A-I intron -    22028664 chr2:128092458(-) A-I intron -    22028664 chr2:128092515(-) A-I intron -    22028664 chr2:128092523(-) A-I intron -    22028664 chr2:128092540(-) A-I intron -    22028664 chr2:128092541(-) A-I intron -    22028664 chr2:128092542(-) A-I intron -    22028664 chr2:128092543(-) A-I intron -    22028664 chr2:128092565(-) A-I intron -    22028664 chr2:128092572(-) A-I intron -    22028664 chr2:128092578(-) A-I intron -    22028664 chr2:128092581(-) A-I intron -    22028664 chr2:128092586(-) A-I intron -    22028664 chr2:128092617(-) A-I intron -    22028664 chr2:128092635(-) A-I intron -    22028664 chr2:128092637(-) A-I intron -    22028664 chr2:128080291(-) A-G intron -    22484847 chr2:128080334(-) A-G intron -    22484847 chr2:128080364(-) A-G intron -    22484847 chr2:128085663(-) A-G intron -    22484847 chr2:128085694(-) A-G intron -    22484847 chr2:128085696(-) A-G intron -    22484847 chr2:128085738(-) A-G intron -    22484847 chr2:128085741(-) A-G intron -    22484847 chr2:128085751(-) A-G intron -    22484847 chr2:128085768(-) A-G intron -    22484847 chr2:128085788(-) A-G intron -    22484847 chr2:128085799(-) A-G intron -    22484847 chr2:128085815(-) A-G intron -    22484847 chr2:128085849(-) A-G intron -    22484847 chr2:128087043(-) A-G intron -    22484847 chr2:128087071(-) A-G intron -    22484847 chr2:128087097(-) A-G intron -    22484847 chr2:128087109(-) A-G intron -    22484847 chr2:128087137(-) A-G intron -    22484847 chr2:128087217(-) A-G intron -    22484847 chr2:128089376(-) A-G intron -    22484847 chr2:128090597(-) A-G intron -    22484847 chr2:128090672(-) A-G intron -    22484847 chr2:128091472(-) A-G intron -    22484847 chr2:128091492(-) A-G intron -    22484847 chr2:128091642(-) A-G intron -    22484847 chr2:128091662(-) A-G intron -    22484847 chr2:128092085(-) A-G intron -    22484847 chr2:128092099(-) A-G intron -    22484847 chr2:128092110(-) A-G intron -    22484847 chr2:128092160(-) A-G intron -    22484847 chr2:128092202(-) A-G intron -    22484847 chr2:128092311(-) A-G intron -    22484847 chr2:128092422(-) A-G intron -    22484847 chr2:128092458(-) A-G intron -    22484847 chr2:128092485(-) A-G intron -    22484847 chr2:128092541(-) A-G intron -    22484847 chr2:128092542(-) A-G intron -    22484847 chr2:128092543(-) A-G intron -    22484847 chr2:128098133(-) A-G intron -    22484847 chr2:128098181(-) A-G intron -    22484847 chr2:128098704(-) A-G intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	MAP3K2	chr2:128058051-128058052(-) m6A 3'UTR       27773535 chr2:128058068-128058069(-) m6A 3'UTR       27773535 chr2:128058090-128058091(-) m6A 3'UTR       27773535 chr2:128060392-128060393(-) m6A 3'UTR       24981863 chr2:128060413-128060414(-) m6A 3'UTR       24981863 chr2:128060436-128060437(-) m6A 3'UTR       24981863 chr2:128060878-128060879(-) m6A 3'UTR       27773535 chr2:128060981-128060982(-) m6A 3'UTR       27773535 chr2:128060999-128061000(-) m6A 3'UTR       27773535 chr2:128061047-128061048(-) m6A 3'UTR       27773535 chr2:128062314-128062315(-) m6A 3'UTR       24981863 chr2:128062352-128062353(-) m6A 3'UTR       24981863 chr2:128062392-128062393(-) m6A 3'UTR       24981863//27773535 chr2:128062401-128062402(-) m6A 3'UTR       24981863//27773535 chr2:128062429-128062430(-) m6A 3'UTR       24981863//27773535 chr2:128063398-128063399(-) m6A 3'UTR       24981863 chr2:128063403-128063404(-) m6A 3'UTR       24981863 chr2:128063418-128063419(-) m6A 3'UTR       24981863//28052920 chr2:128063495-128063496(-) m6A 3'UTR       24981863 chr2:128063796-128063797(-) m6A 3'UTR       24981863 chr2:128063803-128063804(-) m6A 3'UTR       24981863 chr2:128063834-128063835(-) m6A 3'UTR       24981863 chr2:128064366-128064367(-) m6A 3'UTR       27773535 chr2:128064374-128064375(-) m6A 3'UTR       27773535 chr2:128064417-128064418(-) m6A 3'UTR       27773535 chr2:128064450-128064451(-) m6A 3'UTR       27773535 chr2:128064478-128064479(-) m6A 3'UTR       27773535 chr2:128065044-128065045(-) m6A 3'UTR       24981863//27773535//22608085 chr2:128065187-128065188(-) m6A CDS       24284625//24209618//24981863//27773535//27371828//22575960//22608085 chr2:128065202-128065203(-) m6A CDS       24284625//24209618//24981863//27773535//27371828//22575960//22608085 chr2:128065208-128065209(-) m6A CDS       24284625//24209618//24981863//27773535//27371828//22575960//22608085 chr2:128065251-128065252(-) m6A CDS       24284625//24209618//24981863//28052920//27773535//27371828//22575960//22608085 chr2:128065278-128065279(-) m6A CDS       24284625//24209618//24981863//28052920//27773535//27371828//22575960//22608085 chr2:128066166-128066167(-) m6A CDS       24284625//24209618//24981863//27773535//27371828//22575960//22608085 chr2:128066248-128066249(-) m6A CDS       24209618//24981863//27773535//27371828//22608085 chr2:128066266-128066267(-) m6A CDS       24209618//24981863//27773535//27371828//22608085 chr2:128066300-128066301(-) m6A CDS       24209618//27371828 chr2:128079641-128079642(-) m6A CDS       27773535 chr2:128079665-128079666(-) m6A CDS       27773535 chr2:128079789-128079790(-) m6A CDS       27773535 chr2:128079798-128079799(-) m6A CDS       27773535 chr2:128079819-128079820(-) m6A CDS       - chr2:128093478-128093479(-) m6A CDS       24981863 chr2:128093517-128093518(-) m6A CDS       24981863 chr2:128093526-128093527(-) m6A CDS       24981863 chr2:128095330-128095331(-) m6A CDS       24981863 chr2:128096510-128096511(-) m6A CDS       24981863 chr2:128096542-128096543(-) m6A CDS       24981863 chr2:128145272-128145273(-) m6A 5'UTR//intron       27773536 chr2:128145430-128145431(-) m6A 5'UTR//intron       24284625//27773536 chr2:128145831-128145832(-) m6A 5'UTR       27773536 chr2:128145888-128145889(-) m6A 5'UTR       27773536 chr2:128146008-128146009(-) m6A 5'UTR       -

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	MAP3K2	Chromatin K562 cells     - Cytosol Human myelogenous leukemia cell line (K-562)     21613539 Cytosol HCC cell line (HepG2)     - Exosome Blood     - Insoluble cytoplasm HCC cell line (HepG2)     - Membrane HCC cell line (HepG2)     - Nucleolus K562 cells     - Nucleoplasm K562 cells     - Nucleus HCC cell line (HepG2)|HeLa-S3 cells|K562 cells     - Ribosome HEK-293 cells     22199352 Ribosome Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: ebv-miR-BART4-5p
Interactor2: MAP3K2

Evidence Support:

Weak-Evidence	PAR-CLIP
Prediction-Evidence	miRanda//PARalyzer//PITA//Targetscan
Support Database	ViRBase//VIRmiRNA//VmiReg

References:

[1]PMID	22100165	Target region	3'UTR
Source	ViRBase//VIRmiRNA//VmiReg	Interactor1 expression	None
Tissue or cell line	BC-1 cells	Interactor2 expression	Downregulation
Description	Using PAR-CLIP, a technology which allows the direct and transcriptome-wide identification of miRNA targets, we delineate the target sites for all viral and cellular miRNAs expressed in PEL cell lines. The resulting data set revealed that KSHV miRNAs directly target more than 2000 cellular mRNAs, including many involved in pathways relevant to KSHV pathogenesis. Moreover, 58% of these mRNAs are also targeted by EBV miRNAs, via distinct binding sites.Clusters with seed matches to expressed miRNAs were considered candidate target sites. Of the clusters mapping to human 3'UTRs, 69% (BC-1) and 70% (BC-3) had seed matches to expressed miRNAs (Tables S6).Table S6A BC-1 derived 3'UTR clusters with seed matches to expressed miRNAs.

[2]PMID	22291592	Target region	3'UTR
Source	ViRBase//VIRmiRNA//VmiReg	Interactor1 expression	None
Tissue or cell line	EF3D-AGO2 cells//LCL35 cells//LCL-BAC cells//LCL-BAC-D1 cells//LCL-BAC-D3 cells	Interactor2 expression	Downregulation
Description	We identified 7,827 miRNA-interaction sites in 3,492 cellular 3'UTRs. 531 of these sites contained seed matches to viral miRNAs.Table S6: High confidence miRNA-interaction sites in 3'UTRs.We identified an additional 2,972 CDS miRNA-interaction sites present in at least two libraries, 89.7% of which could be assigned to a miRNA (Table S7).