Basic Information:

  VIRBase ID:  

VHID00290296

  Virus:  

Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)

  Host:  

Homo sapiens

  Confidence Score:  

0.8847

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      ebv-miR-BART1-5p:  MIMAT0000999          CLEC2D:  NR_036693     

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Interactor Information:

	Interactor1	Interactor2
Symbol	ebv-miR-BART1-5p	CLEC2D
miRBase Accession/Entrez ID	MIMAT0000999	29121
Organism	Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	CLAX//LLT1//OCIL

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	CLEC2D	chr12:9844887(+) A-I      intronic chr12:9844796(+) A-I      intronic chr12:9841236(+) A-I      intronic chr12:9841204(+) A-I      intronic chr12:9841164(+) A-I      intronic chr12:9841163(+) A-I      intronic chr12:9841148(+) A-I      intronic chr12:9844918(+) A-I      intronic chr12:9844906(+) A-I      intronic chr12:9844859(+) A-I      intronic chr12:9841224(+) A-I      intronic chr12:9844806(+) A-I      intronic chr12:9844920(+) A-I      intronic chr12:9841184(+) A-I      intronic chr12:9844898(+) A-I      intronic chr12:9844916(+) A-I      intronic chr12:9844893(+) A-I      intronic chr12:9841159(+) A-I      intronic chr12:9844812(+) A-I      intronic chr12:9831248(+) A-I      intronic chr12:9841138(+) A-I      intronic chr12:9844915(+) A-I      intronic chr12:9841255(+) A-I      intronic chr12:9831414(+) A-I      intronic chr12:9828064(+) A-I      intronic chr12:9844849(+) A-I      intronic chr12:9831273(+) A-I      intronic chr12:9841176(+) A-I      intronic chr12:9841081(+) A-I      intronic chr12:9841217(+) A-I      intronic chr12:9841221(+) A-I      intronic chr12:9841307(+) A-I      intronic chr12:9844825(+) A-I      intronic chr12:9844833(+) A-I      intronic chr12:9844758(+) A-I      intronic chr12:9831373(+) A-I      intronic chr12:9844958(+) A-I      intronic chr12:9844956(+) A-I      intronic chr12:9844879(+) A-I      intronic chr12:9841168(+) A-I      intronic chr12:9841080(+) A-I      intronic chr12:9844775(+) A-I      intronic chr12:9844871(+) A-I      intronic chr12:9844861(+) A-I      intronic chr12:9841194(+) A-I      intronic chr12:9844945(+) A-I      intronic chr12:9841094(+) A-I      intronic chr12:9844768(+) A-I      intronic chr12:9844970(+) A-I      intronic chr12:9831433(+) A-I      intronic chr12:9831733(+) A-I      intronic chr12:9841113(+) A-I      intronic chr12:9844809(+) A-I      intronic chr12:9841257(+) A-I      intronic chr12:9828058(+) A-I      intronic chr12:9841156(+) A-I      intronic chr12:9841154(+) A-I      intronic chr12:9841208(+) A-I      intronic chr12:9844897(+) A-I      intronic chr12:9823884(+) A-I      intronic chr12:9844857(+) A-I      intronic chr12:9841155(+) A-I      intronic chr12:9844950(+) A-I      intronic chr12:9844811(+) A-I      intronic chr12:9844873(+) A-I      intronic chr12:9841186(+) A-I      intronic chr12:9844858(+) A-I      intronic chr12:9831279(+) A-I      intronic chr12:9841268(+) A-I      intronic chr12:9841261(+) A-I      intronic chr12:9850784(+) A-I      3'UTR chr12:9850755(+) A-I      3'UTR chr12:9850787(+) A-I      3'UTR

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	CLEC2D	chr12:9841080(+) A-I intron -    22327324 chr12:9841186(+) A-I intron -    22327324 chr12:9841208(+) A-I intron -    22327324 chr12:9841217(+) A-I intron -    22327324 chr12:9841261(+) A-I intron -    22327324 chr12:9841268(+) A-I intron -    22327324 chr12:9844809(+) A-I intron -    22327324 chr12:9844857(+) A-I intron -    22327324 chr12:9844879(+) A-I intron -    22327324 chr12:9850784(+) A-I intron -    21960545 chr12:9846013(+) A-G intron -    21725310 chr12:9830325(+) A-T intron -    22484847 chr12:9841080(+) A-G intron -    22484847 chr12:9841094(+) A-G intron -    22484847 chr12:9841113(+) A-G intron -    22484847 chr12:9841138(+) A-G intron -    22484847 chr12:9841154(+) A-G intron -    22484847 chr12:9841155(+) A-G intron -    22484847 chr12:9841168(+) A-G intron -    22484847 chr12:9841176(+) A-G intron -    22484847 chr12:9841186(+) A-G intron -    22484847 chr12:9841194(+) A-G intron -    22484847 chr12:9841208(+) A-G intron -    22484847 chr12:9841255(+) A-G intron -    22484847 chr12:9841257(+) A-G intron -    22484847 chr12:9841261(+) A-G intron -    22484847 chr12:9841268(+) A-G intron -    22484847 chr12:9844811(+) A-G intron -    22484847 chr12:9844812(+) A-G intron -    22484847 chr12:9844825(+) A-G intron -    22484847 chr12:9844849(+) A-G intron -    22484847 chr12:9844857(+) A-G intron -    22484847 chr12:9844871(+) A-G intron -    22484847 chr12:9844879(+) A-G intron -    22484847 chr12:9844915(+) A-G intron -    22484847 chr12:9844945(+) A-G intron -    22484847 chr12:9844956(+) A-G intron -    22484847 chr12:9844958(+) A-G intron -    22484847 chr12:9830330(+) G-T intron -    22484847 chr12:9830409(+) T-C intron -    22484847 chr12:9831273(+) A-G intron -    22484847 chr12:9831279(+) A-G intron -    22484847 chr12:9831373(+) A-G intron -    22484847 chr12:9841081(+) A-G intron -    22484847 chr12:9841156(+) A-G intron -    22484847 chr12:9841159(+) A-G intron -    22484847 chr12:9841319(+) T-A intron -    22484847 chr12:9844758(+) A-G intron -    22484847 chr12:9844768(+) A-G intron -    22484847 chr12:9844819(+) T-C intron -    22484847 chr12:9844833(+) A-G intron -    22484847 chr12:9844858(+) A-G intron -    22484847 chr12:9844950(+) A-G intron -    22484847 chr12:9844970(+) A-G intron -    22484847 chr12:9846481(+) A-G intron -    22484847 chr12:9846526(+) T-C intron -    22484847 chr12:9846527(+) G-A intron -    22484847 chr12:9846539(+) C-T intron -    22484847 chr12:9846690(+) G-T intron -    22484847 chr12:9846692(+) G-C intron -    22484847 chr12:9846693(+) A-C intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	CLEC2D	chr12:9821376-9821377(+) m6A exon//intron       26404942 chr12:9822318-9822319(+) m6A 5'UTR//intron       26404942 chr12:9822358-9822359(+) m6A CDS//intron       26404942 chr12:9822385-9822386(+) m6A CDS//intron       26404942 chr12:9826629-9826630(+) m6A 5'UTR//intron       26404942 chr12:9826643-9826644(+) m6A 5'UTR//intron       26404942 chr12:9847513-9847514(+) m6A 3'UTR       26404942 chr12:9847690-9847691(+) m6A 3'UTR       22608085 chr12:9847817-9847818(+) m6A 3'UTR       22608085 chr12:9847831-9847832(+) m6A 3'UTR       24209618//26404942//22608085 chr12:9847864-9847865(+) m6A 3'UTR       24209618//26404942 chr12:9847870-9847871(+) m6A 3'UTR       24209618//26404942 chr12:9847995-9847996(+) m6A 3'UTR       - chr12:9848001-9848002(+) m6A 3'UTR       22608085 chr12:9848025-9848026(+) m6A 3'UTR       22608085 chr12:9848044-9848045(+) m6A 3'UTR       22608085 chr12:9848096-9848097(+) m6A 3'UTR       25456834//22608085 chr12:9848145-9848146(+) m6A 3'UTR       22608085 chr12:9848273-9848274(+) m6A 3'UTR       22608085 chr12:9848276-9848277(+) Y 3'UTR       26075521 chr12:9848316-9848317(+) m6A 3'UTR       22608085 chr12:9848379-9848380(+) m6A 3'UTR       24981863//22608085 chr12:9848436-9848437(+) m6A 3'UTR       22608085 chr12:9848579-9848580(+) m6A 3'UTR       22608085 chr12:9848586-9848587(+) m6A 3'UTR       22608085 chr12:9848613-9848614(+) m6A 3'UTR       24981863//22608085 chr12:9848631-9848632(+) m6A 3'UTR       24981863 chr12:9848650-9848651(+) m6A 3'UTR       - chr12:9848678-9848679(+) m6A 3'UTR       - chr12:9848737-9848738(+) m6A 3'UTR       - chr12:9848922-9848923(+) m6A 3'UTR       - chr12:9849011-9849012(+) m6A 3'UTR       - chr12:9849059-9849060(+) m6A 3'UTR       - chr12:9849081-9849082(+) m6A 3'UTR       - chr12:9849100-9849101(+) m6A 3'UTR       -

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	CLEC2D	Exosome Blood     - Nucleoplasm K562 cells     -

Related Drug Information:

Resource	Symbol	Drug Name	PubChem ID
RNAactDrug	ebv-miR-BART1-5p	Panobinostat       6918837 Irinotecan       60838 Topotecan       60700 l-685,458       5479543 Palbociclib       5330286 Dovitinib       135398510 Crizotinib       11626560

Interaction Network (The top 100 interactions):

Interactor1: ebv-miR-BART1-5p
Interactor2: CLEC2D

Evidence Support:

Strong-Evidence	Luciferase reporter assay
Weak-Evidence	PAR-CLIP
Prediction-Evidence	PARalyzer
Support Database	ViRBase

References:

[1]PMID	23170179	Target region	3'UTR
Source	ViRBase	Interactor1 expression	Downregulation
Tissue or cell line	EF3D-AGO2 cells//LCL35 cells//LCL-BAC cells//LCL-BAC-D1 cells//LCL-BAC-D3 cells	Interactor2 expression	None
Description	In addition, CLEC2D/LLT1 was identified as a target of BART1 and BART3 by PAR-CLIP.

[2]PMID	23342366	Target region	3'UTR
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	EF3D-AGO2 cells//LCL35 cells//LCL-BAC cells//LCL-BAC-D1 cells//LCL-BAC-D3 cells	Interactor2 expression	None
Description	On the other hand, EBV miRNAs repress cellular proteins, which include p53 up-regulated modulator of apoptosis (PUMA), DICER1, and BIM [96,97,98] (Table 2).

[3]PMID	22291592	Target region	3'UTR
Source	ViRBase	Interactor1 expression	Downregulation
Tissue or cell line	EF3D-AGO2 cells//LCL35 cells//LCL-BAC cells//LCL-BAC-D1 cells//LCL-BAC-D3 cells	Interactor2 expression	None
Description	Of the 12 PAR-CLIP-identified target 3'UTRs tested against EBV miRNAs, 11 3'UTRs (BACH1, CLEC2D, CLIP1, DAZAP2, KDM4B, LY75, OTUD1, PDE7A, PELI1, SP100, ZNF451) resulted in luciferase inhibition following ectopic viral miRNA expression (Figure S3).