Basic Information:

  VIRBase ID:  

VHID00289087

  Virus:  

Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)

  Host:  

Homo sapiens

  Confidence Score:  

0.9530

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      ebv-miR-BART1-5p:  MIMAT0000999          LY75:  NM_002349     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	ebv-miR-BART1-5p	LY75
miRBase Accession/Entrez ID	MIMAT0000999	4065
Organism	Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	CD205//CLEC13B//DEC-205//GP200-MR6//LY-75

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	LY75	chr2:160660988(-) A-I      3'UTR chr2:160660860(-) A-I      3'UTR

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	LY75	chr2:160734936-160734937(-) m6A CDS//exon       27773535 chr2:160735171-160735172(-) m6A CDS//exon       27773535 chr2:160735210-160735211(-) m6A CDS//exon       27773535 chr2:160735220-160735221(-) m6A CDS//exon       27773535 chr2:160735811-160735812(-) m6A CDS//exon       27773535 chr2:160735844-160735845(-) m6A CDS//exon       27773535 chr2:160755280-160755281(-) m6A CDS//exon       - chr2:160758941-160758942(-) m6A exon//intron       27371828 chr2:160758952-160758953(-) m6A exon//intron       27371828 chr2:160758981-160758982(-) m6A exon//intron       27371828 chr2:160759151-160759152(-) m6A exon//intron       28052920//27773535 chr2:160759164-160759165(-) m6A exon//intron       28052920//27773535 chr2:160759205-160759206(-) m6A exon//intron       25456834//24981863//28052920//27773535//27371828 chr2:160759211-160759212(-) m6A exon//intron       25456834//24981863//28052920//27773535//27371828 chr2:160759219-160759220(-) m6A exon//intron       25456834//24981863//28052920//27773535//27371828 chr2:160759231-160759232(-) m6A exon//intron       25456834//24981863//28052920//27773535//27371828 chr2:160759250-160759251(-) m6A exon//intron       25456834//24981863//28052920//27773535//27371828 chr2:160759262-160759263(-) m6A exon//intron       25456834//24981863//28052920//27773535//27371828 chr2:160759279-160759280(-) m6A exon//intron       25456834//24981863//28052920//27773535//27371828 chr2:160759325-160759326(-) m6A exon//intron       25456834//27773535 chr2:160759340-160759341(-) m6A exon//intron       25456834//27773535 chr2:160759430-160759431(-) m6A exon//intron       - chr2:160759548-160759549(-) m6A exon//intron       - chr2:160759585-160759586(-) m6A exon//intron       - chr2:160759956-160759957(-) m6A exon//intron       - chr2:160760008-160760009(-) m6A exon//intron       - chr2:160760020-160760021(-) m6A exon//intron       - chr2:160760089-160760090(-) m6A exon//intron       - chr2:160761186-160761187(-) m6A CDS//exon       27371828

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	LY75	Cytoplasm Nasopharyngeal carcinoma cells     20498841 Exosome Blood     - Nucleus Nasopharyngeal carcinoma cells     20498841

Related Drug Information:

Resource	Symbol	Drug Name	PubChem ID
RNAactDrug	ebv-miR-BART1-5p	Panobinostat       6918837 Irinotecan       60838 Topotecan       60700 l-685,458       5479543 Palbociclib       5330286 Dovitinib       135398510 Crizotinib       11626560

Interaction Network (The top 100 interactions):

Interactor1: ebv-miR-BART1-5p
Interactor2: LY75

Evidence Support:

Strong-Evidence	Dual luciferase reporter assay//Luciferase reporter assay
Weak-Evidence	PAR-CLIP
Prediction-Evidence	cRep//miRanda//PARalyzer//PITA//RNAHybrid//Targetscan
Support Database	RepTar//ViRBase//VIRmiRNA//VmiReg

References:

[1]PMID	23170179	Target region	3'UTR
Source	RepTar//ViRBase	Interactor1 expression	Downregulation
Tissue or cell line	EF3D-AGO2 cells//LCL35 cells//LCL-BAC cells//LCL-BAC-D1 cells//LCL-BAC-D3 cells	Interactor2 expression	None
Description	For each of them, direct interactions between 3'UTR and EBV miRNAs were confirmed by luciferase assay but no functional assay has been performed so far (Table 2).Table 2 Validated targets from high-throughput studies. Shown are cellular targets identified by photoactivatable ribonucleoside enhanced crosslinking and immunoprecipitation (PAR-CLIP), High-throughput sequencing of RNA isolated by crosslinking immunoprecipitation (HITS-CLIP) or RNA immunoprecipitation and microarray (RIP-Chip) techniques. Validation of these targets was done using luciferase reporter assays.

[2]PMID	23342366	Target region	3'UTR
Source	RepTar//ViRBase	Interactor1 expression	None
Tissue or cell line	EF3D-AGO2 cells//LCL35 cells//LCL-BAC cells//LCL-BAC-D1 cells//LCL-BAC-D3 cells	Interactor2 expression	None
Description	On the other hand, EBV miRNAs repress cellular proteins, which include p53 up-regulated modulator of apoptosis (PUMA), DICER1, and BIM [96,97,98] (Table 2).

[3]PMID	22291592	Target region	3'UTR//CDS
Source	RepTar//ViRBase//VIRmiRNA//VmiReg	Interactor1 expression	Downregulation
Tissue or cell line	EF3D-AGO2 cells//LCL35 cells//LCL-BAC cells//LCL-BAC-D1 cells//LCL-BAC-D3 cells	Interactor2 expression	None
Description	We identified 7,827 miRNA-interaction sites in 3,492 cellular 3'UTRs. 531 of these sites contained seed matches to viral miRNAs.Table S6: High confidence miRNA-interaction sites in 3'UTRs.We identified an additional 2,972 CDS miRNA-interaction sites present in at least two libraries, 89.7% of which could be assigned to a miRNA (Table S7).