Basic Information:

  VIRBase ID:  

VHID00284983

  Virus:  

Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)

  Host:  

Homo sapiens

  Confidence Score:  

0.8847

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      ebv-miR-BHRF1-2-3p:  MIMAT0000997          ZNF451:  NM_001031623     

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Interactor Information:

	Interactor1	Interactor2
Symbol	ebv-miR-BHRF1-2-3p	ZNF451
miRBase Accession/Entrez ID	MIMAT0000997	26036
Organism	Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	COASTER//dJ417I1.1

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	ZNF451	chr6:56981282(+) A-I      intronic chr6:57003664(+) A-I      intronic chr6:56983384(+) A-I      intronic chr6:56983442(+) A-I      intronic chr6:56983465(+) A-I      intronic chr6:56983472(+) A-I      intronic chr6:56984092(+) A-I      intronic chr6:56998631(+) A-I      intronic chr6:57025271(+) A-I      intronic chr6:57025212(+) A-I      intronic chr6:56983448(+) A-I      intronic chr6:56983428(+) A-I      intronic chr6:56963005(+) A-I      intronic chr6:56963026(+) A-I      intronic chr6:56963029(+) A-I      intronic chr6:56979803(+) A-I      intronic chr6:56979811(+) A-I      intronic chr6:56979825(+) A-I      intronic chr6:56979901(+) A-I      intronic chr6:56979929(+) A-I      intronic chr6:56979937(+) A-I      intronic chr6:56980047(+) A-I      intronic chr6:56980044(+) A-I      intronic chr6:56980043(+) A-I      intronic chr6:56980019(+) A-I      intronic chr6:57009139(+) A-I      intronic chr6:56979947(+) A-I      intronic chr6:56979951(+) A-I      intronic chr6:56979989(+) A-I      intronic chr6:56980005(+) A-I      intronic chr6:56980009(+) A-I      intronic chr6:56981078(+) A-I      intronic chr6:56981234(+) A-I      intronic chr6:56981274(+) A-I      intronic chr6:57025307(+) A-I      intronic chr6:56983398(+) A-I      intronic chr6:56983415(+) A-I      intronic chr6:56983420(+) A-I      intronic chr6:56983516(+) A-I      intronic chr6:56983534(+) A-I      intronic chr6:56984070(+) A-I      intronic chr6:56984110(+) A-I      intronic chr6:56984138(+) A-I      intronic chr6:56984139(+) A-I      intronic chr6:56984174(+) A-I      intronic chr6:56984203(+) A-I      intronic chr6:56984267(+) A-I      intronic chr6:56984272(+) A-I      intronic chr6:56984310(+) A-I      intronic chr6:56987711(+) A-I      intronic chr6:56987723(+) A-I      intronic chr6:56987766(+) A-I      intronic chr6:56987859(+) A-I      intronic chr6:56987954(+) A-I      intronic chr6:57025316(+) A-I      intronic chr6:57020074(+) A-I      intronic chr6:57020082(+) A-I      intronic chr6:57020085(+) A-I      intronic chr6:57020108(+) A-I      intronic chr6:57020109(+) A-I      intronic chr6:57020119(+) A-I      intronic chr6:57020124(+) A-I      intronic chr6:57020137(+) A-I      intronic chr6:57020190(+) A-I      intronic chr6:57020209(+) A-I      intronic chr6:57025152(+) A-I      intronic chr6:57025154(+) A-I      intronic chr6:57025322(+) A-I      intronic chr6:57025183(+) A-I      intronic chr6:57025354(+) A-I      intronic chr6:57025395(+) A-I      intronic chr6:57028447(+) A-I      intronic chr6:57025355(+) A-I      intronic chr6:57025362(+) A-I      intronic chr6:57025380(+) A-I      intronic chr6:57020180(+) A-I      intronic chr6:57025187(+) A-I      intronic chr6:57025201(+) A-I      intronic chr6:57025218(+) A-I      intronic chr6:57025231(+) A-I      intronic chr6:57025232(+) A-I      intronic chr6:57025298(+) A-I      intronic chr6:57003655(+) A-I      intronic chr6:57003650(+) A-I      intronic chr6:56972880(+) A-I      3'UTR chr6:56972835(+) A-I      3'UTR chr6:57020126(+) A-I      intronic chr6:57025367(+) A-I      intronic chr6:57025249(+) A-I      intronic chr6:56978626(+) A-I      intronic chr6:57025289(+) A-I      intronic chr6:57009543(+) A-I      intronic chr6:57009133(+) A-I      intronic chr6:56979786(+) A-I      intronic chr6:56983380(+) A-I      intronic chr6:56963031(+) A-I      intronic chr6:57020122(+) A-I      intronic chr6:56978549(+) A-I      intronic chr6:57025206(+) A-I      intronic chr6:57025297(+) A-I      intronic chr6:56961245(+) A-I      intronic chr6:57020164(+) A-I      intronic chr6:56983310(+) A-I      intronic chr6:56972843(+) A-I      3'UTR chr6:56979784(+) A-I      intronic chr6:57025394(+) A-I      intronic chr6:56962951(+) A-I      intronic chr6:56963030(+) A-I      intronic chr6:57020123(+) A-I      intronic chr6:56963093(+) A-I      intronic chr6:57018446(+) A-I      intronic chr6:56981304(+) A-I      intronic chr6:56962948(+) A-I      intronic chr6:56983558(+) A-I      intronic chr6:56984166(+) A-I      intronic chr6:57025225(+) A-I      intronic chr6:56983381(+) A-I      intronic chr6:56963013(+) A-I      intronic chr6:56963032(+) A-I      intronic chr6:57004680(+) A-I      intronic chr6:56983410(+) A-I      intronic chr6:56978494(+) A-I      intronic

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	ZNF451	chr6:56962948(+) A-G intron -    22484847 chr6:56962951(+) A-G intron -    22484847 chr6:56963013(+) A-G intron -    22484847 chr6:56963030(+) A-G intron -    22484847 chr6:56963031(+) A-G intron -    22484847 chr6:56963032(+) A-G intron -    22484847 chr6:56963093(+) A-G intron -    22484847 chr6:56971347(+) G-A intron -    22484847 chr6:56972655(+) G-T intron -    22484847 chr6:56972843(+) A-G intron -    22484847 chr6:56975716(+) C-T intron -    22484847 chr6:56976183(+) G-T intron -    22484847 chr6:56978494(+) A-G intron -    22484847 chr6:56978537(+) A-G intron -    22484847 chr6:56978549(+) A-G intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	ZNF451	chr6:56955010-56955011(+) m6A 5'UTR//exon//intron       24981863 chr6:56955049-56955050(+) m6A 5'UTR//exon//intron       24981863 chr6:56955058-56955059(+) m6A CDS//exon//intron       24981863 chr6:56965541-56965542(+) m6A CDS//exon//intron       24981863//22608085 chr6:56965557-56965558(+) m6A CDS//exon//intron       24284625//24981863//22575960//22608085 chr6:56965569-56965570(+) m6A CDS//exon//intron       24284625//24981863//22575960//22608085 chr6:56965613-56965614(+) m6A CDS//intron       24284625//24981863//22575960//22608085 chr6:56965619-56965620(+) m6A CDS//intron       24284625//24209618//24981863//22575960//22608085 chr6:56965646-56965647(+) m6A CDS//intron       24284625//24209618//24981863//22575960//22608085 chr6:56965772-56965773(+) m6A CDS//intron       24284625//24209618//24981863//22608085 chr6:56965857-56965858(+) m6A CDS//intron       24284625//24981863//22608085 chr6:56965875-56965876(+) m6A CDS//intron       24284625//24981863//22608085 chr6:56965922-56965923(+) m6A CDS//intron       24284625//24981863//22608085 chr6:56966011-56966012(+) m6A CDS//intron       24284625//24981863//22608085 chr6:56966071-56966072(+) m6A CDS//intron       24284625//24981863//22608085 chr6:56966154-56966155(+) m6A CDS//intron       24284625//24209618//25456834//24981863//22575960//22608085 chr6:56966188-56966189(+) m6A CDS//intron       24284625//24209618//24981863//22575960//22608085 chr6:56966214-56966215(+) m6A CDS//intron       24284625//24209618//24981863//22575960//22608085 chr6:56966235-56966236(+) m6A CDS//intron       24284625//24209618//24981863//22575960//22608085 chr6:56966272-56966273(+) m6A CDS//intron       24284625//24981863//22575960//22608085 chr6:56966289-56966290(+) m6A CDS//intron       24284625//24981863//22575960//22608085 chr6:56966416-56966417(+) m6A CDS//intron       24284625//24209618//24981863//22575960//22608085 chr6:56966427-56966428(+) m6A CDS//intron       24284625//24209618//24981863//22575960//22608085 chr6:56966532-56966533(+) m6A CDS//intron       24284625//24209618//25456834//24981863//27773535//22575960//22608085 chr6:56966541-56966542(+) m6A CDS//intron       24284625//24209618//25456834//24981863//22575960//22608085 chr6:56966574-56966575(+) m6A CDS//intron       24284625//24209618//25456834//24981863//22575960//22608085 chr6:56966621-56966622(+) m6A CDS//intron       24284625//24209618//25456834//24981863//22575960//22608085 chr6:56966671-56966672(+) m6A CDS//intron       24284625//24981863//22575960//22608085 chr6:56966700-56966701(+) m6A CDS//intron       24981863//22575960//22608085 chr6:56966824-56966825(+) m6A CDS//intron       24209618//24981863//22608085 chr6:56966832-56966833(+) m6A CDS//intron       24209618//24981863//22608085 chr6:56966862-56966863(+) m6A CDS//intron       24209618//24981863//22608085 chr6:56966996-56966997(+) m6A 3'UTR//intron       24284625//24209618//24981863//22575960//22608085 chr6:56967042-56967043(+) m6A 3'UTR//intron       24284625//24209618//24981863//22575960//22608085 chr6:56967071-56967072(+) m6A 3'UTR//intron       24284625//24209618//24981863//22575960//22608085 chr6:56967104-56967105(+) m6A 3'UTR//intron       24284625//24209618//24981863//22575960//22608085 chr6:56967123-56967124(+) m6A 3'UTR//intron       24284625//24981863//22575960//22608085 chr6:56967171-56967172(+) m6A 3'UTR//intron       24981863//22575960//22608085 chr6:56967248-56967249(+) m6A 3'UTR//intron       24981863//22608085 chr6:56967255-56967256(+) m6A 3'UTR//intron       24981863//22608085 chr6:56967304-56967305(+) m6A 3'UTR//intron       24981863//22608085 chr6:56967457-56967458(+) m6A 3'UTR//exon//intron       24284625//24981863//22575960//22608085 chr6:56967565-56967566(+) m6A 3'UTR//exon//intron       24284625//24209618//24981863//22575960//22608085 chr6:56967582-56967583(+) m6A 3'UTR//exon//intron       24284625//24209618//24981863//22575960//22608085 chr6:56967635-56967636(+) m6A 3'UTR//exon//intron       24284625//24209618//24981863//22575960//22608085 chr6:56967748-56967749(+) m6A 3'UTR//intron       24284625//24209618//24981863//22575960//22608085 chr6:56967807-56967808(+) m6A 3'UTR//intron       24284625//24209618//24981863//22608085 chr6:56967823-56967824(+) m6A 3'UTR//intron       24284625//24209618//24981863//22608085 chr6:56967935-56967936(+) m6A 3'UTR//intron       24209618//24981863//22575960//22608085 chr6:56967944-56967945(+) m6A 3'UTR//intron       24209618//24981863//22575960//22608085 chr6:56967958-56967959(+) m6A 3'UTR//intron       24981863//22575960//22608085 chr6:56968068-56968069(+) m6A 3'UTR//intron       22608085 chr6:56968203-56968204(+) m6A 3'UTR//intron       24981863//22608085 chr6:56968259-56968260(+) m6A 3'UTR//intron       24981863//22608085 chr6:56968308-56968309(+) m6A 3'UTR//intron       24981863//22608085 chr6:56968441-56968442(+) m6A 3'UTR//intron       24981863//22608085 chr6:56993582-56993583(+) m6A 3'UTR//5'UTR//CDS//exon//intron       24981863 chr6:56993606-56993607(+) m6A 3'UTR//5'UTR//CDS//exon//intron       24981863 chr6:56993617-56993618(+) m6A 3'UTR//5'UTR//CDS//exon//intron       24981863 chr6:56997847-56997848(+) m6A 3'UTR//CDS//exon//intron       24981863 chr6:56997868-56997869(+) m6A 3'UTR//CDS//exon//intron       24981863 chr6:57011908-57011909(+) m6A CDS//exon//intron       24284625 chr6:57011919-57011920(+) Tm CDS//exon//intron       - chr6:57012005-57012006(+) m6A CDS//exon//intron       24284625//24981863//22608085 chr6:57012037-57012038(+) m6A CDS//exon//intron       24284625//24981863//22608085 chr6:57012105-57012106(+) m6A CDS//exon//intron       24284625//24981863//22608085 chr6:57012170-57012171(+) m6A CDS//exon//intron       24284625//24981863//22608085 chr6:57012293-57012294(+) m6A CDS//exon//intron       24284625//24209618//24981863//22608085 chr6:57012363-57012364(+) m6A CDS//exon//intron       24284625//24209618//24981863//27773535//22575960//22608085 chr6:57012485-57012486(+) m6A CDS//exon//intron       24284625//24209618//24981863//26404942//27773535//22575960//22608085 chr6:57012550-57012551(+) m6A CDS//exon//intron       24284625//24209618//24981863//26404942//27773535//22575960//22608085 chr6:57012590-57012591(+) m6A CDS//exon//intron       24284625//24209618//24981863//26404942//27773535//22575960//22608085 chr6:57012748-57012749(+) m6A CDS//exon//intron       24284625//24209618//24981863//22575960//22608085 chr6:57012830-57012831(+) m6A CDS//exon//intron       24284625//24981863//22608085 chr6:57012983-57012984(+) m6A CDS//exon//intron       24284625//24981863//26404942//27773535//22608085 chr6:57012989-57012990(+) m6A CDS//exon//intron       24284625//24981863//26404942//27773535//22608085 chr6:57013001-57013002(+) m6A CDS//exon//intron       24284625//24981863//27773535//22608085 chr6:57013028-57013029(+) m6A CDS//exon//intron       24284625//24981863//22608085 chr6:57013132-57013133(+) m6A CDS//exon//intron       24284625//24981863//22608085 chr6:57013188-57013189(+) m6A CDS//exon//intron       24284625//24209618//24981863//22575960//22608085 chr6:57013315-57013316(+) m6A CDS//exon//intron       24284625//24981863//27773535//22575960//22608085 chr6:57013333-57013334(+) m6A CDS//exon//intron       24284625//24981863//27773535//22575960//22608085 chr6:57013368-57013369(+) m6A CDS//exon//intron       24284625//24981863//27773535//22575960//22608085 chr6:57013411-57013412(+) m6A CDS//exon//intron       24284625//24981863//27773535//22608085 chr6:57013417-57013418(+) m6A CDS//exon//intron       24284625//24981863//27773535//22608085 chr6:57013428-57013429(+) m6A CDS//exon//intron       24284625//24981863//27773535//22608085 chr6:57013446-57013447(+) m6A CDS//exon//intron       24284625//24981863//27773535//22608085 chr6:57015557-57015558(+) m6A CDS//exon//intron       24981863//22608085 chr6:57018754-57018755(+) m6A CDS//exon//intron       27773535 chr6:57018779-57018780(+) m6A CDS//exon//intron       27773535 chr6:57034669-57034670(+) m6A 3'UTR       24981863//27773535 chr6:57034676-57034677(+) m6A 3'UTR       24981863//27773535 chr6:57034766-57034767(+) m6A 3'UTR       24981863//27773535 chr6:57034808-57034809(+) m6A 3'UTR       24981863//27773535 chr6:57034875-57034876(+) m6A 3'UTR       24981863//27773535

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	ebv-miR-BHRF1-2-3p	Exosome K562 cells     24460325
	ZNF451	Chromatin K562 cells     - Cytosol HCC cell line (HepG2)|K562 cells     - Exosome Blood     - Membrane K562 cells     - Nucleolus K562 cells     - Nucleoplasm K562 cells     - Nucleus HCC cell line (HepG2)|HeLa-S3 cells|K562 cells     -

Interaction Network (The top 100 interactions):

Interactor1: ebv-miR-BHRF1-2-3p
Interactor2: ZNF451

Evidence Support:

Strong-Evidence	Dual luciferase reporter assay
Weak-Evidence	PAR-CLIP
Prediction-Evidence	PARalyzer
Support Database	ViRBase//VIRmiRNA//VmiReg

References:

[1]PMID	23170179	Target region	3'UTR
Source	ViRBase	Interactor1 expression	Downregulation
Tissue or cell line	EF3D-AGO2 cells//LCL35 cells//LCL-BAC cells//LCL-BAC-D1 cells//LCL-BAC-D3 cells	Interactor2 expression	None
Description	For each of them, direct interactions between 3'UTR and EBV miRNAs were confirmed by luciferase assay but no functional assay has been performed so far (Table 2).Table 2 Validated targets from high-throughput studies. Shown are cellular targets identified by photoactivatable ribonucleoside enhanced crosslinking and immunoprecipitation (PAR-CLIP), High-throughput sequencing of RNA isolated by crosslinking immunoprecipitation (HITS-CLIP) or RNA immunoprecipitation and microarray (RIP-Chip) techniques. Validation of these targets was done using luciferase reporter assays.

[2]PMID	22291592	Target region	3'UTR
Source	ViRBase//VIRmiRNA//VmiReg	Interactor1 expression	Downregulation
Tissue or cell line	EF3D-AGO2 cells//LCL35 cells//LCL-BAC cells//LCL-BAC-D1 cells//LCL-BAC-D3 cells	Interactor2 expression	None
Description	For each of them, direct interactions between 3'UTR and EBV miRNAs were confirmed by luciferase assay but no functional assay has been performed so far (Table 2).Table 2 Validated targets from high-throughput studies. Shown are cellular targets identified by photoactivatable ribonucleoside enhanced crosslinking and immunoprecipitation (PAR-CLIP), High-throughput sequencing of RNA isolated by crosslinking immunoprecipitation (HITS-CLIP) or RNA immunoprecipitation and microarray (RIP-Chip) techniques. Validation of these targets was done using luciferase reporter assays.