Basic Information:

  VIRBase ID:  

VHID00279855

  Virus:  

Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)

  Host:  

Homo sapiens

  Confidence Score:  

0.6002

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      ebv-miR-BHRF1-2-5p:  MIMAT0000996          GNAS:  NR_132273     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	ebv-miR-BHRF1-2-5p	GNAS
miRBase Accession/Entrez ID	MIMAT0000996	2778
Organism	Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	AHO//C20orf45//GNAS1//GPSA//GSA//GSP//NESP//PITA3//POH//SCG6//SgVI

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	GNAS	chr20:57468506(+) A-I      intronic chr20:57480322(+) A-I      intronic chr20:57436943(+) A-I      intronic

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	GNAS	chr20:57414775-57414776(+) m6A 5'UTR       25456834//24981863 chr20:57414832-57414833(+) m6A 5'UTR       25456834//24981863 chr20:57414877-57414878(+) m6A 5'UTR       25456834//24981863 chr20:57414903-57414904(+) m6A 5'UTR       25456834//24981863 chr20:57414908-57414909(+) m6A 5'UTR       25456834//24981863 chr20:57415435-57415436(+) m6A CDS       25456834 chr20:57415491-57415492(+) m6A CDS       25456834 chr20:57415503-57415504(+) m6A CDS       25456834 chr20:57415527-57415528(+) m6A CDS       25456834 chr20:57415539-57415540(+) m6A CDS       25456834 chr20:57415551-57415552(+) m6A CDS       25456834 chr20:57415575-57415576(+) m6A CDS       25456834 chr20:57415753-57415754(+) m6A CDS       25456834//24981863 chr20:57415768-57415769(+) m6A CDS       25456834//24981863 chr20:57415874-57415875(+) m6A CDS       25456834//24981863 chr20:57416414-57416415(+) m6A exon//intron       25456834 chr20:57419117-57419118(+) m6A exon//intron       - chr20:57427772-57427773(+) m6A 5'UTR//intron       - chr20:57427912-57427913(+) m6A 5'UTR//intron       25456834 chr20:57427960-57427961(+) m6A 5'UTR//intron       25456834 chr20:57427997-57427998(+) m6A 5'UTR//intron       25456834 chr20:57428042-57428043(+) m6A 5'UTR//intron       25456834 chr20:57428104-57428105(+) m6A 5'UTR//intron       25456834 chr20:57428164-57428165(+) m6A 5'UTR//intron       - chr20:57428204-57428205(+) m6A 5'UTR//intron       - chr20:57428867-57428868(+) m6A CDS//exon//intron       - chr20:57428986-57428987(+) m6A CDS//exon//intron       25456834 chr20:57429189-57429190(+) m6A CDS//exon//intron       - chr20:57429237-57429238(+) m6A CDS//exon//intron       - chr20:57429296-57429297(+) m6A CDS//exon//intron       - chr20:57429372-57429373(+) m6A CDS//exon//intron       - chr20:57429562-57429563(+) m6A CDS//exon//intron       - chr20:57463533-57463534(+) m6A exon//intron       24284625 chr20:57463581-57463582(+) m6A exon//intron       24284625 chr20:57464228-57464229(+) m6A 5'UTR//exon//intron       25456834 chr20:57464482-57464483(+) m6A exon//intron       24209618 chr20:57465823-57465824(+) m6A exon//intron       24209618 chr20:57466797-57466798(+) m6A CDS//exon//intron       24284625//24209618//24981863//27773535//27371828//22575960 chr20:57466805-57466806(+) m6A CDS//exon//intron       24284625//24209618//24981863//27773535//27371828//22575960 chr20:57466812-57466813(+) m6A CDS//exon//intron       24284625//24209618//24981863//27773535//27371828//22575960 chr20:57466853-57466854(+) Am CDS//exon//intron       - chr20:57466878-57466879(+) m6A CDS//exon//intron       24284625//24209618//24981863//26404942//27773535//27371828//22575960 chr20:57467077-57467078(+) m6A exon//intron       24284625//24209618//27773535//22575960 chr20:57470235-57470236(+) m6A exon//intron       24284625 chr20:57470585-57470586(+) m6A exon//intron       22575960 chr20:57470590-57470591(+) m6A exon//intron       22575960 chr20:57470603-57470604(+) m6A exon//intron       22575960 chr20:57470647-57470648(+) m6A exon//intron       24981863//22575960 chr20:57472503-57472504(+) m6A exon//intron       24981863//27773535//22575960 chr20:57472508-57472509(+) m6A exon//intron       24981863//27773535//22575960 chr20:57473926-57473927(+) m6A exon//intron       24209618//24981863 chr20:57474009-57474010(+) m6A 3'UTR//CDS//exon//intron       24209618//24981863//22575960 chr20:57475094-57475095(+) m6A 3'UTR//exon//intron       24209618//24981863//22575960 chr20:57475191-57475192(+) m6A 3'UTR//exon//intron       24284625//24981863 chr20:57475353-57475354(+) m6A 3'UTR//exon//intron       24284625 chr20:57475361-57475362(+) m6A 3'UTR//exon//intron       24284625 chr20:57477534-57477535(+) Tm intron       - chr20:57478485-57478486(+) m6A exon//intron       - chr20:57478608-57478609(+) m6A 3'UTR//CDS//exon//intron       24981863//22575960 chr20:57478617-57478618(+) m6A 3'UTR//CDS//exon//intron       24981863//22575960 chr20:57478784-57478785(+) m6A 3'UTR//CDS//exon//intron       24981863//26404942//22575960 chr20:57478799-57478800(+) m6A 3'UTR//CDS//exon//intron       24981863//26404942//22575960 chr20:57478831-57478832(+) Tm 3'UTR//CDS//exon//intron       - chr20:57484446-57484447(+) m6A 3'UTR//CDS//exon//intron       22575960 chr20:57484462-57484463(+) m6A 3'UTR//CDS//exon//intron       22575960 chr20:57485084-57485085(+) Am 3'UTR//CDS//exon       - chr20:57485094-57485095(+) m6A 3'UTR//CDS//exon       26404942//22575960 chr20:57485099-57485100(+) Tm 3'UTR//CDS//exon       - chr20:57485409-57485410(+) m6A 3'UTR//CDS//exon       22575960 chr20:57485801-57485802(+) m6A 3'UTR//CDS//exon       26404942//27773535//22575960 chr20:57485810-57485811(+) m6A 3'UTR//CDS//exon       26404942//27773535//22575960 chr20:57485891-57485892(+) m6A 3'UTR//exon       26404942//27773535 chr20:57486022-57486023(+) m6A 3'UTR//exon       24981863//26404942//27773535 chr20:57486140-57486141(+) m6A 3'UTR//exon       24981863//27773535//22608085 chr20:57486153-57486154(+) m6A 3'UTR//exon       24981863//27773535//22608085 chr20:57486176-57486177(+) m6A 3'UTR//exon       24981863//27773535//22608085

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	ebv-miR-BHRF1-2-5p	Exosome B cell line (Raji)     21505438
	GNAS	Chromatin K562 cells     - Cytoplasm Nasopharyngeal carcinoma cells     20498841 Cytoplasm Nasopharyngeal carcinoma cells     20498841 Cytoplasm Nasopharyngeal carcinoma cells     20498841 Cytoplasm Nasopharyngeal carcinoma cells     20498841 Cytosol Human myelogenous leukemia cell line (K-562)     21613539 Cytosol HCC cell line (HepG2)|HeLa-S3 cells|K562 cells     - Exosome Blood     - Insoluble cytoplasm HCC cell line (HepG2)|K562 cells     - Membrane HCC cell line (HepG2)|K562 cells     - Nucleolus K562 cells     - Nucleoplasm K562 cells     - Nucleus Nasopharyngeal carcinoma cells     20498841 Nucleus Nasopharyngeal carcinoma cells     20498841 Nucleus Nasopharyngeal carcinoma cells     20498841 Nucleus Nasopharyngeal carcinoma cells     20498841 Nucleus HCC cell line (HepG2)|HeLa-S3 cells|K562 cells     - Ribosome Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: ebv-miR-BHRF1-2-5p
Interactor2: GNAS

Evidence Support:

Weak-Evidence	PAR-CLIP
Prediction-Evidence	miRanda//PARalyzer//Targetscan
Support Database	ViRBase//VIRmiRNA//VmiReg

References:

PMID	22291592	Target region	3'UTR
Source	ViRBase//VIRmiRNA//VmiReg	Interactor1 expression	None
Tissue or cell line	EF3D-AGO2 cells//LCL35 cells//LCL-BAC cells//LCL-BAC-D1 cells//LCL-BAC-D3 cells	Interactor2 expression	Downregulation
Description	We identified 7,827 miRNA-interaction sites in 3,492 cellular 3'UTRs. 531 of these sites contained seed matches to viral miRNAs.Table S6: High confidence miRNA-interaction sites in 3'UTRs.We identified an additional 2,972 CDS miRNA-interaction sites present in at least two libraries, 89.7% of which could be assigned to a miRNA (Table S7).