Basic Information:

  VIRBase ID:  

VHID00279059

  Virus:  

Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)

  Host:  

Homo sapiens

  Confidence Score:  

0.9523

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      ebv-miR-BHRF1-1:  MIMAT0000995          NAA30:  NM_001011713     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	ebv-miR-BHRF1-1	NAA30
miRBase Accession/Entrez ID	MIMAT0000995	122830
Organism	Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	C14orf35//MAK3//Mak3p//NAT12//NAT12P

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	NAA30	chr14:57873738(+) A-I      intronic chr14:57873753(+) A-I      intronic chr14:57873723(+) A-I      intronic chr14:57873757(+) A-I      intronic chr14:57873737(+) A-I      intronic chr14:57873741(+) A-I      intronic chr14:57873697(+) A-I      intronic chr14:57864412(+) A-I      intronic chr14:57865301(+) A-I      intronic chr14:57865302(+) A-I      intronic chr14:57865321(+) A-I      intronic chr14:57865315(+) A-I      intronic chr14:57873634(+) A-I      intronic chr14:57864416(+) A-I      intronic chr14:57865199(+) A-I      intronic chr14:57864564(+) A-I      intronic chr14:57864555(+) A-I      intronic chr14:57864623(+) A-I      intronic chr14:57870920(+) A-I      intronic chr14:57865450(+) A-I      intronic chr14:57864390(+) A-I      intronic chr14:57865212(+) A-I      intronic chr14:57865195(+) A-I      intronic chr14:57864457(+) A-I      intronic chr14:57865664(+) A-I      intronic chr14:57870961(+) A-I      intronic chr14:57864387(+) A-I      intronic chr14:57873659(+) A-I      intronic chr14:57873746(+) A-I      intronic chr14:57864467(+) A-I      intronic chr14:57870946(+) A-I      intronic chr14:57865476(+) A-I      intronic chr14:57876733(+) A-I      3'UTR

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	NAA30	chr14:57857272-57857273(+) m6A 5'UTR       25456834//24981863 chr14:57857326-57857327(+) m6A 5'UTR       24209618//25456834//24981863 chr14:57857412-57857413(+) m6A 5'UTR       24209618//25456834//24981863 chr14:57857947-57857948(+) m6A CDS//intron       24284625//24209618//25456834//24981863//22608085 chr14:57858116-57858117(+) m6A CDS//intron       24284625//24209618//25456834//24981863//26404942//22575960//22608085 chr14:57858178-57858179(+) m6A CDS//intron       24284625//24209618//25456834//24981863//26404942//22575960//22608085 chr14:57858361-57858362(+) m6A CDS//intron       24284625//24981863//26404942//22608085 chr14:57858385-57858386(+) m6A CDS//intron       24284625//24981863//26404942//22608085 chr14:57866590-57866591(+) m6A CDS//intron       24209618//24981863//22608085 chr14:57876108-57876109(+) m6A CDS       24209618//24981863//22608085 chr14:57876137-57876138(+) m6A 3'UTR//CDS       24284625//24981863//22608085 chr14:57876218-57876219(+) m6A 3'UTR//CDS       24981863//26404942//22608085 chr14:57876237-57876238(+) m6A 3'UTR       24981863//26404942//22608085 chr14:57876251-57876252(+) m6A 3'UTR       24981863//26404942//22608085

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	ebv-miR-BHRF1-1	Exosome Jurkat-derived T cell line (J77cl20)     21505438
	NAA30	Cytosol Human myelogenous leukemia cell line (K-562)     21613539 Cytosol HCC cell line (HepG2)     - Exosome Blood     - Nucleus HCC cell line (HepG2)|HeLa-S3 cells     - Ribosome Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: ebv-miR-BHRF1-1
Interactor2: NAA30

Evidence Support:

Strong-Evidence	Dual luciferase reporter assay//Luciferase reporter assay
Weak-Evidence	PAR-CLIP
Prediction-Evidence	miRanda//PARalyzer//Targetscan
Support Database	ViRBase//VmiReg

References:

[1]PMID	23170179	Target region	3'UTR
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	EF3D-AGO2 cells//LCL35 cells//LCL-BAC cells//LCL-BAC-D1 cells//LCL-BAC-D3 cells	Interactor2 expression	Downregulation
Description	For each of them, direct interactions between 3'UTR and EBV miRNAs were confirmed by luciferase assay but no functional assay has been performed so far (Table 2).Table 2 Validated targets from high-throughput studies. Shown are cellular targets identified by photoactivatable ribonucleoside enhanced crosslinking and immunoprecipitation (PAR-CLIP), High-throughput sequencing of RNA isolated by crosslinking immunoprecipitation (HITS-CLIP) or RNA immunoprecipitation and microarray (RIP-Chip) techniques. Validation of these targets was done using luciferase reporter assays.

[2]PMID	22291592	Target region	3'UTR
Source	ViRBase//VmiReg	Interactor1 expression	None
Tissue or cell line	EF3D-AGO2 cells//LCL35 cells//LCL-BAC cells//LCL-BAC-D1 cells//LCL-BAC-D3 cells	Interactor2 expression	Downregulation
Description	We identified 7,827 miRNA-interaction sites in 3,492 cellular 3'UTRs. 531 of these sites contained seed matches to viral miRNAs.Table S6: High confidence miRNA-interaction sites in 3'UTRs.We identified an additional 2,972 CDS miRNA-interaction sites present in at least two libraries, 89.7% of which could be assigned to a miRNA (Table S7).