Basic Information:

  VIRBase ID:  

VHID00278042

  Virus:  

Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)

  Host:  

Homo sapiens

  Confidence Score:  

0.8589

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      ebv-miR-BHRF1-1:  MIMAT0000995          KDM4B:  NM_001370094     

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Interactor Information:

	Interactor1	Interactor2
Symbol	ebv-miR-BHRF1-1	KDM4B
miRBase Accession/Entrez ID	MIMAT0000995	23030
Organism	Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	JMJD2B//TDRD14B

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	KDM4B	chr19:4990155(+) A-I      intronic chr19:5139033(+) A-I      intronic chr19:5105709(+) A-I      intronic chr19:5105683(+) A-I      intronic chr19:4990180(+) A-I      intronic chr19:4990186(+) A-I      intronic chr19:4990203(+) A-I      intronic chr19:5006704(+) A-I      intronic chr19:5006705(+) A-I      intronic chr19:5006734(+) A-I      intronic chr19:5105513(+) A-I      intronic chr19:5105556(+) A-I      intronic chr19:5105578(+) A-I      intronic chr19:5030182(+) A-I      intronic chr19:5029047(+) A-I      intronic chr19:4987190(+) A-I      intronic chr19:4976102(+) A-I      intronic chr19:5021762(+) A-I      intronic chr19:5021744(+) A-I      intronic chr19:5021697(+) A-I      intronic chr19:5021544(+) A-I      intronic chr19:5021451(+) A-I      intronic chr19:5021331(+) A-I      intronic chr19:5110280(+) A-I      intronic chr19:5015627(+) A-I      intronic chr19:4980713(+) A-I      intronic chr19:5044624(+) A-I      intronic chr19:4993238(+) A-I      intronic chr19:5002882(+) A-I      intronic chr19:4976071(+) A-I      intronic chr19:4976101(+) A-I      intronic chr19:4976166(+) A-I      intronic chr19:4976180(+) A-I      intronic chr19:4976181(+) A-I      intronic chr19:4979040(+) A-I      intronic chr19:4979840(+) A-I      intronic chr19:4979842(+) A-I      intronic chr19:5101168(+) A-I      intronic chr19:5101161(+) A-I      intronic chr19:5101139(+) A-I      intronic chr19:5101079(+) A-I      intronic chr19:5101030(+) A-I      intronic chr19:5100661(+) A-I      intronic chr19:5100513(+) A-I      intronic chr19:4979899(+) A-I      intronic chr19:4980103(+) A-I      intronic chr19:5003371(+) A-I      intronic chr19:5003372(+) A-I      intronic chr19:5003398(+) A-I      intronic chr19:5044659(+) A-I      intronic chr19:5003471(+) A-I      intronic chr19:4993721(+) A-I      intronic chr19:5097365(+) A-I      intronic chr19:5097361(+) A-I      intronic chr19:4990280(+) A-I      intronic chr19:4990210(+) A-I      intronic chr19:4990209(+) A-I      intronic chr19:4990160(+) A-I      intronic chr19:5003472(+) A-I      intronic chr19:4990081(+) A-I      intronic chr19:4989429(+) A-I      intronic chr19:4989410(+) A-I      intronic chr19:4989374(+) A-I      intronic chr19:5006529(+) A-I      intronic chr19:5044685(+) A-I      intronic chr19:5089919(+) A-I      intronic chr19:5084906(+) A-I      intronic chr19:5084727(+) A-I      intronic chr19:5084702(+) A-I      intronic chr19:5084696(+) A-I      intronic chr19:5044658(+) A-I      intronic chr19:5061615(+) A-I      intronic chr19:5008038(+) A-I      intronic chr19:5007799(+) A-I      intronic chr19:5006725(+) A-I      intronic chr19:5006637(+) A-I      intronic chr19:5110342(+) A-I      intronic chr19:4982785(+) A-I      intronic chr19:4982760(+) A-I      intronic chr19:4990281(+) A-I      intronic chr19:4991415(+) A-I      intronic chr19:4992522(+) A-I      intronic chr19:4980552(+) A-I      intronic chr19:5006534(+) A-I      intronic chr19:4980590(+) A-I      intronic chr19:4980601(+) A-I      intronic chr19:4980609(+) A-I      intronic chr19:4980700(+) A-I      intronic chr19:4993244(+) A-I      intronic chr19:4993245(+) A-I      intronic chr19:4993268(+) A-I      intronic chr19:4993433(+) A-I      intronic chr19:4993722(+) A-I      intronic chr19:4993858(+) A-I      intronic chr19:4994328(+) A-I      intronic chr19:4994453(+) A-I      intronic chr19:4995419(+) A-I      intronic chr19:4990136(+) A-I      intronic chr19:4994467(+) A-I      intronic chr19:5149455(+) A-I      intronic chr19:5002855(+) A-I      intronic chr19:4992538(+) A-I      intronic chr19:5110227(+) A-I      intronic chr19:4993157(+) A-I      intronic chr19:5015628(+) A-I      intronic chr19:5030187(+) A-I      intronic chr19:4995473(+) A-I      intronic chr19:4992723(+) A-I      intronic chr19:5021063(+) A-I      intronic chr19:4990181(+) A-I      intronic chr19:4993820(+) A-I      intronic chr19:5110368(+) A-I      intronic chr19:5034034(+) A-I      intronic chr19:5138478(+) A-I      intronic chr19:5100603(+) A-I      intronic chr19:5143283(+) A-I      intronic chr19:4973339(+) A-I      intronic chr19:5101179(+) A-I      intronic chr19:5100578(+) A-I      intronic chr19:5105650(+) A-I      intronic chr19:5140667(+) A-I      intronic chr19:4990163(+) A-I      intronic chr19:4993274(+) A-I      intronic chr19:5029727(+) A-I      intronic chr19:5084048(+) A-I      intronic chr19:5101070(+) A-I      intronic chr19:5002272(+) A-I      intronic chr19:5143073(+) A-I      intronic chr19:4973223(+) A-I      intronic chr19:5054264(+) A-I      intronic chr19:5101440(+) A-I      intronic chr19:5100970(+) A-I      intronic chr19:4973300(+) A-I      intronic chr19:5084964(+) A-I      intronic chr19:4980104(+) A-I      intronic chr19:4975759(+) A-I      intronic chr19:5026446(+) A-I      intronic chr19:4990222(+) A-I      intronic chr19:5124859(+) A-I      intronic chr19:4990861(+) A-I      intronic chr19:4994407(+) A-I      intronic chr19:5015356(+) A-I      intronic chr19:4975743(+) A-I      intronic chr19:5100652(+) A-I      intronic chr19:5034065(+) A-I      intronic chr19:4993206(+) A-I      intronic chr19:5097331(+) A-I      intronic chr19:5110292(+) A-I      intronic chr19:5002944(+) A-I      intronic chr19:5023991(+) A-I      intronic chr19:5084926(+) A-I      intronic chr19:5024800(+) A-I      intronic chr19:5034905(+) A-I      intronic chr19:4994159(+) A-I      intronic chr19:4985224(+) A-I      intronic chr19:4976046(+) A-I      intronic chr19:5149409(+) A-I      intronic chr19:4990985(+) A-I      intronic chr19:5044610(+) A-I      intronic chr19:4982157(+) A-I      intronic chr19:4979196(+) A-I      intronic chr19:4978371(+) A-I      intronic chr19:4989683(+) A-I      intronic chr19:4974568(+) A-I      intronic chr19:4990120(+) A-I      intronic chr19:5034014(+) A-I      intronic chr19:5100556(+) A-I      intronic chr19:5023938(+) A-I      intronic chr19:4989442(+) A-I      intronic chr19:4993415(+) A-I      intronic chr19:5146831(+) A-I      intronic chr19:4980126(+) A-I      intronic chr19:4992482(+) A-I      intronic chr19:5100539(+) A-I      intronic chr19:4992598(+) A-I      intronic chr19:4974020(+) A-I      intronic chr19:5124647(+) A-I      intronic chr19:5023828(+) A-I      intronic chr19:5110425(+) A-I      intronic chr19:5139118(+) A-I      intronic chr19:5100615(+) A-I      intronic chr19:5105543(+) A-I      intronic chr19:5021745(+) A-I      intronic chr19:5034060(+) A-I      intronic chr19:5100487(+) A-I      intronic chr19:5084951(+) A-I      intronic chr19:4980673(+) A-I      intronic chr19:5084043(+) A-I      intronic chr19:5139135(+) A-I      intronic chr19:4980564(+) A-I      intronic chr19:5138569(+) A-I      intronic chr19:4973329(+) A-I      intronic chr19:5101031(+) A-I      intronic chr19:5097529(+) A-I      intronic chr19:4973231(+) A-I      intronic chr19:5045501(+) A-I      intronic chr19:5001183(+) A-I      intronic chr19:5029105(+) A-I      intronic chr19:5021453(+) A-I      intronic chr19:5021008(+) A-I      intronic chr19:5101174(+) A-I      intronic chr19:5146818(+) A-I      intronic chr19:4976113(+) A-I      intronic chr19:4994399(+) A-I      intronic chr19:4973380(+) A-I      intronic chr19:5045666(+) A-I      intronic chr19:5138644(+) A-I      intronic chr19:5110435(+) A-I      intronic chr19:5101071(+) A-I      intronic chr19:4980057(+) A-I      intronic chr19:5100689(+) A-I      intronic chr19:4993309(+) A-I      intronic chr19:4992563(+) A-I      intronic chr19:5110385(+) A-I      intronic chr19:4990972(+) A-I      intronic chr19:5101153(+) A-I      intronic chr19:5084991(+) A-I      intronic chr19:5101154(+) A-I      intronic chr19:4980600(+) A-I      intronic chr19:5100644(+) A-I      intronic chr19:5101081(+) A-I      intronic chr19:4975865(+) A-I      intronic chr19:5002851(+) A-I      intronic chr19:5139117(+) A-I      intronic chr19:5101072(+) A-I      intronic chr19:4990150(+) A-I      intronic chr19:4973383(+) A-I      intronic chr19:4993341(+) A-I      intronic chr19:5021786(+) A-I      intronic chr19:4979281(+) A-I      intronic chr19:4994231(+) A-I      intronic chr19:4974156(+) A-I      intronic chr19:5138984(+) A-I      intronic chr19:5060400(+) A-I      intronic chr19:4980615(+) A-I      intronic chr19:4974598(+) A-I      intronic chr19:4975690(+) A-I      intronic chr19:5110220(+) A-I      intronic chr19:4993211(+) A-I      intronic chr19:5045522(+) A-I      intronic chr19:5100532(+) A-I      intronic chr19:5139034(+) A-I      intronic chr19:5042273(+) A-I      intronic chr19:5105577(+) A-I      intronic chr19:4975833(+) A-I      intronic chr19:4974596(+) A-I      intronic chr19:5089940(+) A-I      intronic chr19:4982885(+) A-I      intronic chr19:4994495(+) A-I      intronic chr19:4975629(+) A-I      intronic chr19:5143223(+) A-I      intronic chr19:5021749(+) A-I      intronic chr19:5138642(+) A-I      intronic chr19:4989489(+) A-I      intronic chr19:5006713(+) A-I      intronic chr19:4980535(+) A-I      intronic chr19:5140647(+) A-I      intronic chr19:4976110(+) A-I      intronic chr19:5024862(+) A-I      intronic chr19:5100474(+) A-I      intronic chr19:5039355(+) A-I      intronic chr19:4979973(+) A-I      intronic chr19:5101493(+) A-I      intronic chr19:5097498(+) A-I      intronic chr19:5002035(+) A-I      intronic chr19:4990258(+) A-I      intronic chr19:5110226(+) A-I      intronic chr19:4979956(+) A-I      intronic chr19:5029103(+) A-I      intronic chr19:5097362(+) A-I      intronic chr19:5002825(+) A-I      intronic chr19:5021059(+) A-I      intronic chr19:4987142(+) A-I      intronic chr19:5015404(+) A-I      intronic chr19:4994167(+) A-I      intronic chr19:4989803(+) A-I      intronic chr19:5143259(+) A-I      intronic chr19:5101113(+) A-I      intronic chr19:4979936(+) A-I      intronic chr19:5138563(+) A-I      intronic chr19:4990874(+) A-I      intronic chr19:4980674(+) A-I      intronic chr19:5084051(+) A-I      intronic chr19:4979173(+) A-I      intronic chr19:5101086(+) A-I      intronic chr19:5002555(+) A-I      intronic chr19:4974099(+) A-I      intronic chr19:5149385(+) A-I      intronic chr19:5039368(+) A-I      intronic chr19:5149361(+) A-I      intronic chr19:4973420(+) A-I      intronic

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	KDM4B	chr19:5139117(+) A-I intron -    22327324 chr19:4976113(+) A-G intron -    22484847 chr19:4993383(+) A-G intron -    22484847 chr19:5097356(+) A-G intron -    22484847 chr19:5100591(+) A-G intron -    22484847 chr19:5100603(+) A-G intron -    22484847 chr19:5101086(+) A-G intron -    22484847 chr19:5101113(+) A-G intron -    22484847 chr19:5101414(+) A-G intron -    22484847 chr19:5105543(+) A-G intron -    22484847 chr19:5105577(+) A-G intron -    22484847 chr19:5110262(+) A-G intron -    22484847 chr19:5110281(+) A-G intron -    22484847 chr19:5139034(+) A-G intron -    22484847 chr19:5139051(+) A-G intron -    22484847 chr19:5139117(+) A-G intron -    22484847 chr19:5139118(+) A-G intron -    22484847 chr19:5139119(+) A-G intron -    22484847 chr19:5140647(+) A-G intron -    22484847 chr19:5146831(+) A-G intron -    22484847 chr19:5153054(+) C-A intron -    22484847 chr19:5153055(+) T-A intron -    22484847 chr19:5153056(+) C-A intron -    22484847 chr19:5153057(+) C-A intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	KDM4B	chr19:5016334-5016335(+) m6A 5'UTR//intron       24284625 chr19:5032914-5032915(+) m6A CDS       24284625 chr19:5032929-5032930(+) m6A CDS       24284625 chr19:5032980-5032981(+) m6A CDS       24284625 chr19:5077457-5077458(+) m1A CDS//exon       26863196 chr19:5077984-5077985(+) m6A exon//intron       - chr19:5077991-5077992(+) m6A exon//intron       - chr19:5078044-5078045(+) m6A exon//intron       - chr19:5078253-5078254(+) m6A exon//intron       - chr19:5078389-5078390(+) m6A exon//intron       - chr19:5078484-5078485(+) m6A exon//intron       - chr19:5078712-5078713(+) m6A exon//intron       - chr19:5078764-5078765(+) m6A exon//intron       - chr19:5078941-5078942(+) m6A exon//intron       - chr19:5078969-5078970(+) m6A exon//intron       - chr19:5080906-5080907(+) m6A exon//intron       25456834 chr19:5080915-5080916(+) m6A exon//intron       25456834 chr19:5080946-5080947(+) m6A exon//intron       25456834 chr19:5080988-5080989(+) m6A exon//intron       25456834 chr19:5110726-5110727(+) m6A CDS//exon       - chr19:5110741-5110742(+) m6A CDS//exon       - chr19:5111977-5111978(+) m6A 3'UTR//intron       24981863 chr19:5112064-5112065(+) m6A 3'UTR//intron       24981863 chr19:5112253-5112254(+) m6A 3'UTR//intron       24981863 chr19:5112272-5112273(+) m6A 3'UTR//intron       24981863 chr19:5112280-5112281(+) m6A 3'UTR//intron       24981863 chr19:5112292-5112293(+) m6A 3'UTR//intron       24981863 chr19:5112300-5112301(+) m6A 3'UTR//intron       24981863 chr19:5112331-5112332(+) m6A 3'UTR//intron       24981863 chr19:5113332-5113333(+) m6A 3'UTR//intron       24981863 chr19:5119148-5119149(+) m6A CDS//exon//intron       24981863 chr19:5119165-5119166(+) m6A CDS//exon//intron       24981863 chr19:5119182-5119183(+) m6A CDS//exon//intron       24981863 chr19:5119190-5119191(+) Tm CDS//exon//intron       - chr19:5119210-5119211(+) m6A CDS//exon//intron       24209618//25456834//24981863 chr19:5119707-5119708(+) m6A CDS//exon       24209618//25456834//24981863 chr19:5131136-5131137(+) m1A CDS//exon       26863196 chr19:5131459-5131460(+) m6A CDS//exon       - chr19:5131529-5131530(+) m6A CDS//exon       - chr19:5133971-5133972(+) m6A CDS//exon       - chr19:5135479-5135480(+) m6A CDS//exon       24284625 chr19:5135523-5135524(+) m6A CDS//exon       24284625 chr19:5140547-5140548(+) m6A 3'UTR//intron       - chr19:5140569-5140570(+) m6A 3'UTR//intron       - chr19:5140594-5140595(+) m6A 3'UTR//intron       - chr19:5140643-5140644(+) m6A 3'UTR//intron       - chr19:5141021-5141022(+) m6A 3'UTR//intron       25456834 chr19:5141056-5141057(+) m6A 3'UTR//intron       25456834 chr19:5141070-5141071(+) m6A 3'UTR//intron       25456834 chr19:5151442-5151443(+) m6A CDS//exon       24284625//25456834//24981863//22608085 chr19:5151460-5151461(+) m6A CDS//exon       24284625//25456834//24981863//22608085 chr19:5151523-5151524(+) m6A 3'UTR//exon       24284625//24209618//25456834//24981863//22575960//22608085 chr19:5151695-5151696(+) m6A 3'UTR//exon       24209618//25456834//24981863//27371828//22575960//22608085 chr19:5151768-5151769(+) m6A 3'UTR//exon       24209618//25456834//24981863//27371828 chr19:5152002-5152003(+) m6A 3'UTR       - chr19:5152078-5152079(+) m6A 3'UTR       - chr19:5152095-5152096(+) m6A 3'UTR       - chr19:5152531-5152532(+) Cm 3'UTR       -

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	ebv-miR-BHRF1-1	Exosome Jurkat-derived T cell line (J77cl20)     21505438
	KDM4B	Chromatin K562 cells     - Cytosol Human myelogenous leukemia cell line (K-562)     21613539 Cytosol K562 cells     - Exosome Blood     - Membrane K562 cells     - Nucleolus K562 cells     - Nucleoplasm K562 cells     - Nucleus HCC cell line (HepG2)|HeLa-S3 cells|K562 cells     -

Interaction Network (The top 100 interactions):

Interactor1: ebv-miR-BHRF1-1
Interactor2: KDM4B

Evidence Support:

Strong-Evidence	Dual luciferase reporter assay
Weak-Evidence	PAR-CLIP
Support Database	VmiReg

References:

PMID	22291592	Target region	3'UTR
Source	VmiReg	Interactor1 expression	None
Tissue or cell line	293T cells	Interactor2 expression	Downregulation
Description	Of the 12 PAR-CLIP-identified target 3'UTRs tested against EBV miRNAs, 11 3'UTRs (BACH1, CLEC2D, CLIP1, DAZAP2, KDM4B, LY75, OTUD1, PDE7A, PELI1, SP100, ZNF451) resulted in luciferase inhibition following ectopic viral miRNA expression (Figure S3).