Basic Information:

  VIRBase ID:  

VHID00277824

  Virus:  

Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)

  Host:  

Homo sapiens

  Confidence Score:  

0.9523

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      ebv-miR-BHRF1-1:  MIMAT0000995          SCRN1:  NM_014766     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	ebv-miR-BHRF1-1	SCRN1
miRBase Accession/Entrez ID	MIMAT0000995	9805
Organism	Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	SES1

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	SCRN1	chr7:29992088(-) A-I      intronic chr7:29989243(-) A-I      intronic chr7:29988868(-) A-I      intronic chr7:29988681(-) A-I      intronic chr7:29979744(-) A-I      intronic chr7:29979720(-) A-I      intronic chr7:29994425(-) A-I      intronic chr7:29979708(-) A-I      intronic chr7:29979642(-) A-I      intronic chr7:29977821(-) A-I      intronic chr7:29979026(-) A-I      intronic chr7:29977815(-) A-I      intronic chr7:30018870(-) A-I      intronic chr7:29979600(-) A-I      intronic chr7:30022217(-) A-I      intronic chr7:29977808(-) A-I      intronic chr7:29976660(-) A-I      intronic chr7:29976654(-) A-I      intronic chr7:29977813(-) A-I      intronic chr7:29976610(-) A-I      intronic chr7:30018922(-) A-I      intronic chr7:30022242(-) A-I      intronic chr7:30022592(-) A-I      intronic chr7:30022725(-) A-I      intronic chr7:30025666(-) A-I      intronic chr7:30025668(-) A-I      intronic chr7:29977812(-) A-I      intronic chr7:29994418(-) A-I      intronic chr7:29994386(-) A-I      intronic chr7:29979640(-) A-I      intronic chr7:29979586(-) A-I      intronic chr7:29992182(-) A-I      intronic chr7:29977866(-) A-I      intronic chr7:30023649(-) A-I      intronic chr7:29977741(-) A-I      intronic chr7:29979005(-) A-I      intronic chr7:29979733(-) A-I      intronic chr7:30014488(-) A-I      intronic chr7:29979523(-) A-I      intronic chr7:29976418(-) A-I      intronic chr7:29976473(-) A-I      intronic chr7:30022199(-) A-I      intronic chr7:29988742(-) A-I      intronic chr7:30025846(-) A-I      intronic chr7:29989090(-) A-I      intronic chr7:30022551(-) A-I      intronic chr7:30025740(-) A-I      intronic chr7:30014517(-) A-I      intronic chr7:30014571(-) A-I      intronic chr7:30022566(-) A-I      intronic chr7:29979015(-) A-I      intronic chr7:29978993(-) A-I      intronic chr7:29977677(-) A-I      intronic chr7:30022570(-) A-I      intronic chr7:30024290(-) A-I      intronic chr7:29979644(-) A-I      intronic chr7:30022472(-) A-I      intronic chr7:29977713(-) A-I      intronic chr7:29972630(-) A-I      intronic chr7:30022223(-) A-I      intronic chr7:30022061(-) A-I      intronic chr7:30023700(-) A-I      intronic chr7:29979077(-) A-I      intronic chr7:29989397(-) A-I      intronic chr7:30010992(-) A-I      intronic chr7:29989421(-) A-I      intronic chr7:29977850(-) A-I      intronic chr7:30023759(-) A-I      intronic chr7:29989382(-) A-I      intronic chr7:30022204(-) A-I      intronic chr7:29976406(-) A-I      intronic chr7:29994466(-) A-I      intronic chr7:30023587(-) A-I      intronic chr7:29978928(-) A-I      intronic chr7:30026479(-) A-I      intronic chr7:30022213(-) A-I      intronic chr7:30023656(-) A-I      intronic chr7:29978922(-) A-I      intronic chr7:30022619(-) A-I      intronic chr7:30011149(-) A-I      intronic chr7:29994434(-) A-I      intronic chr7:30014424(-) A-I      intronic chr7:29979006(-) A-I      intronic chr7:30022593(-) A-I      intronic chr7:30025606(-) A-I      intronic chr7:30022175(-) A-I      intronic chr7:29989488(-) A-I      intronic chr7:29979749(-) A-I      intronic chr7:30022159(-) A-I      intronic chr7:29989329(-) A-I      intronic chr7:29977809(-) A-I      intronic chr7:29978890(-) A-I      intronic chr7:29972057(-) A-I      intronic chr7:30025805(-) A-I      intronic chr7:29989265(-) A-I      intronic chr7:30022748(-) A-I      intronic chr7:29979700(-) A-I      intronic chr7:29978996(-) A-I      intronic chr7:29989550(-) A-I      intronic chr7:29992071(-) A-I      intronic chr7:29994437(-) A-I      intronic chr7:29977783(-) A-I      intronic

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	SCRN1	chr7:30001688(-) A-I intron -    21960545 chr7:30023700(-) A-I intron -    21960545 chr7:29977712(-) A-G intron -    22484847 chr7:29977717(-) A-G intron -    22484847 chr7:29977783(-) A-G intron -    22484847 chr7:29977809(-) A-G intron -    22484847 chr7:29978967(-) A-G intron -    22484847 chr7:29978969(-) A-G intron -    22484847 chr7:29979005(-) A-G intron -    22484847 chr7:29979006(-) A-G intron -    22484847 chr7:29979523(-) A-G intron -    22484847 chr7:29979641(-) A-G intron -    22484847 chr7:29988742(-) A-G intron -    22484847 chr7:29989260(-) A-G intron -    22484847 chr7:29989265(-) A-G intron -    22484847 chr7:29989382(-) A-G intron -    22484847 chr7:29989397(-) A-G intron -    22484847 chr7:29989488(-) A-G intron -    22484847 chr7:29989550(-) A-G intron -    22484847 chr7:29992071(-) A-G intron -    22484847 chr7:29992224(-) A-G intron -    22484847 chr7:29992294(-) A-G intron -    22484847 chr7:29992308(-) T-C intron -    22484847 chr7:29994434(-) A-G intron -    22484847 chr7:29994437(-) A-G intron -    22484847 chr7:30014424(-) A-G intron -    22484847 chr7:30014488(-) A-G intron -    22484847 chr7:30014517(-) A-G intron -    22484847 chr7:30014544(-) A-G intron -    22484847 chr7:30014549(-) A-G intron -    22484847 chr7:30014570(-) A-G intron -    22484847 chr7:30014571(-) A-G intron -    22484847 chr7:30022061(-) A-G intron -    22484847 chr7:30022159(-) A-G intron -    22484847 chr7:30022174(-) A-G intron -    22484847 chr7:30022177(-) A-G intron -    22484847 chr7:30022204(-) A-G intron -    22484847 chr7:30022213(-) A-G intron -    22484847 chr7:30022223(-) A-G intron -    22484847 chr7:30022551(-) A-G intron -    22484847 chr7:30022570(-) A-G intron -    22484847 chr7:30022593(-) A-G intron -    22484847 chr7:30022611(-) A-G intron -    22484847 chr7:30022619(-) A-G intron -    22484847 chr7:30022726(-) A-G intron -    22484847 chr7:30022741(-) A-G intron -    22484847 chr7:30022748(-) A-G intron -    22484847 chr7:30023587(-) A-G intron -    22484847 chr7:30024950(-) C-T intron -    22484847 chr7:30025606(-) A-G intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	SCRN1	chr7:29960650-29960651(-) m6A 3'UTR       24981863//26404942 chr7:29960719-29960720(-) m6A 3'UTR       24981863//26404942 chr7:29960756-29960757(-) m6A 3'UTR       24981863//26404942 chr7:29960809-29960810(-) m6A 3'UTR       24981863 chr7:29962147-29962148(-) Cm 3'UTR       - chr7:29962344-29962345(-) m6A 3'UTR       24981863//26404942//27773535 chr7:29962442-29962443(-) m6A 3'UTR       24981863//26404942//27773535 chr7:29962467-29962468(-) m6A 3'UTR       24981863//26404942//27773535 chr7:29962543-29962544(-) m6A 3'UTR       27773535 chr7:29962554-29962555(-) m6A 3'UTR       27773535 chr7:29962600-29962601(-) Y 3'UTR       26075521 chr7:29963260-29963261(-) m6A 3'UTR       26404942//27773535 chr7:29963283-29963284(-) m6A 3'UTR       26404942//27773535 chr7:29963440-29963441(-) m6A 3'UTR       24284625//24209618//26404942//27773535 chr7:29963510-29963511(-) m6A 3'UTR       24284625//24209618//24981863//28052920//26404942//27773535//22608085 chr7:29963539-29963540(-) m6A 3'UTR       24284625//24209618//24981863//28052920//26404942//27773535//22608085 chr7:29963618-29963619(-) m6A CDS       24284625//24209618//24981863//28052920//26404942//27773535//27371828//22575960//22608085 chr7:29963636-29963637(-) m6A CDS       24284625//24209618//24981863//28052920//26404942//27773535//27371828//22575960//22608085 chr7:29980427-29980428(-) m6A CDS       22575960 chr7:29983772-29983773(-) m6A CDS//exon       24981863//27773535 chr7:29986847-29986848(-) m6A CDS//intron       24981863//27773535 chr7:29994880-29994881(-) m6A CDS//exon       24981863//26404942//27773535 chr7:29994919-29994920(-) m6A CDS//exon       26404942//27773535 chr7:29994942-29994943(-) m6A 5'UTR//CDS//exon       27773535 chr7:30008542-30008543(-) m6A CDS//exon//intron       27773535 chr7:30008745-30008746(-) m6A 5'UTR//intron       27773536 chr7:30008776-30008777(-) m6A 5'UTR//intron       27773536 chr7:30009494-30009495(-) m6A 5'UTR//intron       27773535 chr7:30029337-30029338(-) m6A 5'UTR//intron       24981863//27371828//27773536

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	ebv-miR-BHRF1-1	Exosome Jurkat-derived T cell line (J77cl20)     21505438
	SCRN1	Cytosol Human myelogenous leukemia cell line (K-562)     21613539 Exosome Blood     - Ribosome HEK-293 cells     22199352 Ribosome Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: ebv-miR-BHRF1-1
Interactor2: SCRN1

Evidence Support:

Strong-Evidence	Dual luciferase reporter assay//Luciferase reporter assay
Weak-Evidence	PAR-CLIP
Prediction-Evidence	miRanda//PARalyzer//Targetscan
Support Database	ViRBase//VIRmiRNA//VmiReg

References:

[1]PMID	23170179	Target region	3'UTR
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	EF3D-AGO2 cells//LCL35 cells//LCL-BAC cells//LCL-BAC-D1 cells//LCL-BAC-D3 cells	Interactor2 expression	Downregulation
Description	For each of them, direct interactions between 3'UTR and EBV miRNAs were confirmed by luciferase assay but no functional assay has been performed so far (Table 2).Table 2 Validated targets from high-throughput studies. Shown are cellular targets identified by photoactivatable ribonucleoside enhanced crosslinking and immunoprecipitation (PAR-CLIP), High-throughput sequencing of RNA isolated by crosslinking immunoprecipitation (HITS-CLIP) or RNA immunoprecipitation and microarray (RIP-Chip) techniques. Validation of these targets was done using luciferase reporter assays.

[2]PMID	22291592	Target region	3'UTR
Source	ViRBase//VIRmiRNA//VmiReg	Interactor1 expression	None
Tissue or cell line	EF3D-AGO2 cells//LCL35 cells//LCL-BAC cells//LCL-BAC-D1 cells//LCL-BAC-D3 cells	Interactor2 expression	Downregulation
Description	We selected three 3'UTRs (GUF1, NAT12, and SCRN1) that were identified as high confidence targets for miR-BHRF1-1 for further analysis by luciferase reporter assays.