Basic Information:

  VIRBase ID:  

VHID00277590

  Virus:  

Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)

  Host:  

Homo sapiens

  Confidence Score:  

0.9612

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      ebv-miR-BHRF1-1:  MIMAT0000995          TP53:  NM_001276697     

      *It may take a few minutes to display results.

Interactor Information:

	Interactor1	Interactor2
Symbol	ebv-miR-BHRF1-1	TP53
miRBase Accession/Entrez ID	MIMAT0000995	7157
Organism	Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	BCC7//BMFS5//LFS1//P53//TRP53

RNA Editing:

Resource	Symbol	Editing Position
Lncediting	TP53	chr17: 7588904     chr17: 7588877

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	TP53	chr17:7574250(-) A-I      intronic chr17:7573520(-) A-I      intronic chr17:7573351(-) A-I      intronic chr17:7573387(-) A-I      intronic chr17:7573391(-) A-I      intronic chr17:7573398(-) A-I      intronic chr17:7573399(-) A-I      intronic chr17:7573478(-) A-I      intronic chr17:7574292(-) A-I      intronic chr17:7574227(-) A-I      intronic chr17:7573369(-) A-I      intronic chr17:7575291(-) A-I      intronic chr17:7575306(-) A-I      intronic chr17:7575453(-) A-I      intronic chr17:7576230(-) A-I      intronic chr17:7576261(-) A-I      intronic chr17:7576264(-) A-I      intronic chr17:7576361(-) A-I      intronic chr17:7587661(-) A-I      intronic chr17:7576380(-) A-I      intronic chr17:7576396(-) A-I      intronic chr17:7576405(-) A-I      intronic chr17:7576421(-) A-I      intronic chr17:7586541(-) A-I      intronic chr17:7575466(-) A-I      intronic chr17:7575516(-) A-I      intronic chr17:7575536(-) A-I      intronic chr17:7575592(-) A-I      intronic chr17:7575608(-) A-I      intronic chr17:7576437(-) A-I      intronic chr17:7583952(-) A-I      intronic chr17:7574403(-) A-I      intronic chr17:7574462(-) A-I      intronic chr17:7574463(-) A-I      intronic chr17:7574479(-) A-I      intronic chr17:7574730(-) A-I      intronic chr17:7574756(-) A-I      intronic chr17:7574767(-) A-I      intronic chr17:7574782(-) A-I      intronic chr17:7574800(-) A-I      intronic chr17:7574811(-) A-I      intronic chr17:7574821(-) A-I      intronic chr17:7574826(-) A-I      intronic chr17:7588904(-) A-I      intronic chr17:7588877(-) A-I      intronic chr17:7587669(-) A-I      intronic chr17:7587624(-) A-I      intronic chr17:7587598(-) A-I      intronic chr17:7587574(-) A-I      intronic chr17:7587205(-) A-I      intronic chr17:7583956(-) A-I      intronic chr17:7587199(-) A-I      intronic chr17:7575775(-) A-I      intronic chr17:7575868(-) A-I      intronic chr17:7575939(-) A-I      intronic chr17:7576110(-) A-I      intronic chr17:7576184(-) A-I      intronic chr17:7573279(-) A-I      intronic chr17:7573315(-) A-I      intronic chr17:7573355(-) A-I      intronic chr17:7586686(-) A-I      intronic chr17:7573435(-) A-I      intronic chr17:7574228(-) A-I      intronic chr17:7574305(-) A-I      intronic chr17:7572048(-) A-I      3'UTR chr17:7572107(-) A-I      3'UTR chr17:7571943(-) A-I      3'UTR chr17:7571926(-) A-I      3'UTR chr17:7571944(-) A-I      3'UTR chr17:7574393(-) A-I      intronic chr17:7586338(-) A-I      intronic chr17:7587302(-) A-I      intronic chr17:7574688(-) A-I      intronic chr17:7574296(-) A-I      intronic chr17:7587561(-) A-I      intronic chr17:7587082(-) A-I      intronic chr17:7574520(-) A-I      intronic chr17:7584245(-) A-I      intronic chr17:7587693(-) A-I      intronic chr17:7574572(-) A-I      intronic chr17:7587621(-) A-I      intronic chr17:7575299(-) A-I      intronic chr17:7576319(-) A-I      intronic chr17:7574311(-) A-I      intronic chr17:7584291(-) A-I      intronic chr17:7573395(-) A-I      intronic chr17:7574545(-) A-I      intronic chr17:7575897(-) A-I      intronic chr17:7587676(-) A-I      intronic chr17:7587514(-) A-I      intronic chr17:7587473(-) A-I      intronic chr17:7587534(-) A-I      intronic chr17:7587218(-) A-I      intronic chr17:7574298(-) A-I      intronic chr17:7585684(-) A-I      intronic chr17:7587701(-) A-I      intronic chr17:7573459(-) A-I      intronic chr17:7573390(-) A-I      intronic chr17:7587545(-) A-I      intronic chr17:7582810(-) A-I      intronic chr17:7574715(-) A-I      intronic chr17:7575858(-) A-I      intronic chr17:7573473(-) A-I      intronic chr17:7574318(-) A-I      intronic chr17:7587126(-) A-I      intronic chr17:7572004(-) A-I      3'UTR chr17:7574265(-) A-I      intronic chr17:7587660(-) A-I      intronic chr17:7571913(-) A-I      3'UTR chr17:7572141(-) A-I      3'UTR chr17:7587472(-) A-I      intronic chr17:7575233(-) A-I      intronic chr17:7573467(-) A-I      intronic chr17:7572026(-) A-I      3'UTR chr17:7587671(-) A-I      intronic chr17:7587663(-) A-I      intronic chr17:7573334(-) A-I      intronic chr17:7587200(-) A-I      intronic chr17:7574341(-) A-I      intronic chr17:7575528(-) A-I      intronic chr17:7574913(-) A-I      intronic chr17:7575356(-) A-I      intronic chr17:7574407(-) A-I      intronic chr17:7587537(-) A-I      intronic chr17:7575244(-) A-I      intronic chr17:7576064(-) A-I      intronic chr17:7587301(-) A-I      intronic chr17:7587668(-) A-I      intronic chr17:7586352(-) A-I      intronic chr17:7574783(-) A-I      intronic chr17:7574517(-) A-I      intronic chr17:7573472(-) A-I      intronic chr17:7575465(-) A-I      intronic chr17:7573360(-) A-I      intronic chr17:7587190(-) A-I      intronic chr17:7575947(-) A-I      intronic chr17:7587198(-) A-I      intronic chr17:7576006(-) A-I      intronic chr17:7587499(-) A-I      intronic chr17:7574757(-) A-I      intronic chr17:7582804(-) A-I      intronic chr17:7582053(-) A-I      intronic chr17:7574539(-) A-I      intronic chr17:7574258(-) A-I      intronic chr17:7573454(-) A-I      intronic chr17:7574354(-) A-I      intronic chr17:7574328(-) A-I      intronic chr17:7573361(-) A-I      intronic chr17:7576027(-) A-I      intronic chr17:7587525(-) A-I      intronic chr17:7587645(-) A-I      intronic chr17:7575777(-) A-I      intronic chr17:7574547(-) A-I      intronic chr17:7571931(-) A-I      3'UTR

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	TP53	chr17:7587671(-) A-I intron -    22327324 chr17:7572004(-) A-G exon 3'UTR    22484847 chr17:7572148(-) C-T exon 3'UTR    22484847 chr17:7572154(-) C-T exon 3'UTR    22484847 chr17:7572173(-) C-T exon 3'UTR    22484847 chr17:7573360(-) A-G intron -    22484847 chr17:7573361(-) A-G intron -    22484847 chr17:7574354(-) A-G intron -    22484847 chr17:7574547(-) A-G intron -    22484847 chr17:7587198(-) A-G intron -    22484847 chr17:7587301(-) A-G intron -    22484847 chr17:7587302(-) A-G intron -    22484847 chr17:7587472(-) A-G intron -    22484847 chr17:7587645(-) A-G intron -    22484847 chr17:7587663(-) A-G intron -    22484847 chr17:7587668(-) A-G intron -    22484847 chr17:7587671(-) A-G intron -    22484847 chr17:7587676(-) A-G intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	TP53	chr17:7569558-7569559(-) m6A CDS//intron       24981863//27773535 chr17:7571749-7571750(-) m6A 3'UTR//intron       24981863//27773535 chr17:7571942-7571943(-) m6A 3'UTR//intron       27773535 chr17:7572719-7572720(-) m6A 3'UTR//intron       26404942 chr17:7572771-7572772(-) m6A 3'UTR//intron       - chr17:7572804-7572805(-) m6A 3'UTR//intron       - chr17:7572843-7572844(-) m6A 3'UTR//intron       - chr17:7572930-7572931(-) m6A 3'UTR//CDS//intron       27773535//27371828 chr17:7572949-7572950(-) m6A 3'UTR//CDS//intron       27773535//27371828 chr17:7572962-7572963(-) m6A 3'UTR//CDS//intron       27773535//27371828 chr17:7573979-7573980(-) m6A 3'UTR//CDS//intron       27371828 chr17:7576546-7576547(-) m6A 3'UTR//CDS//intron       27371828 chr17:7576650-7576651(-) m6A CDS//intron       24981863//27371828 chr17:7576882-7576883(-) m6A CDS//intron       24981863//27371828//27773536 chr17:7577095-7577096(-) m6A CDS//intron       - chr17:7578511-7578512(-) m6A 5'UTR//CDS//exon       24284625//27773535 chr17:7579334-7579335(-) m6A CDS//exon//intron       24284625 chr17:7579382-7579383(-) m6A CDS//exon//intron       24284625 chr17:7579516-7579517(-) m6A CDS//exon//intron       24284625//22575960 chr17:7579533-7579534(-) m6A CDS//exon//intron       24284625//22575960 chr17:7579709-7579710(-) m6A 5'UTR//CDS//exon//intron       24284625//22575960 chr17:7579720-7579721(-) m6A 5'UTR//CDS//exon//intron       24284625//22575960 chr17:7579840-7579841(-) m6A 5'UTR//CDS//exon//intron       24284625//27371828//27773536 chr17:7579850-7579851(-) m6A 5'UTR//CDS//exon//intron       24284625//27371828//27773536 chr17:7579860-7579861(-) m6A 5'UTR//CDS//exon//intron       24284625//27371828//27773536 chr17:7579932-7579933(-) m6A 5'UTR//exon//intron       24284625//27371828//27773536 chr17:7580702-7580703(-) m6A 5'UTR//intron       24284625//24981863//27371828//27773536 chr17:7588791-7588792(-) m6A intron       27371828//27773536 chr17:7588861-7588862(-) m6A intron       27371828//27773536 chr17:7588979-7588980(-) m6A intron       24284625//25456834//24981863//27371828//22608085 chr17:7589074-7589075(-) m6A intron       24284625//25456834//24981863//22608085 chr17:7589175-7589176(-) m6A intron       24284625//24981863//27773535//22608085 chr17:7589398-7589399(-) m6A intron       24284625//22608085 chr17:7589550-7589551(-) m6A intron       22608085 chr17:7590754-7590755(-) m6A 5'UTR//exon       24284625//25456834//24981863 chr17:7590831-7590832(-) m6A 5'UTR       25456834//24981863

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	ebv-miR-BHRF1-1	Exosome Jurkat-derived T cell line (J77cl20)     21505438
	TP53	Cytosol HCC cell line (HepG2)|K562 cells     - Exosome Blood     - Membrane HCC cell line (HepG2)|K562 cells     - Nucleolus K562 cells     - Nucleoplasm K562 cells     - Nucleus HeLa-S3 cells     - Ribosome HEK-293 cells     22199352 Ribosome Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: ebv-miR-BHRF1-1
Interactor2: TP53

Evidence Support:

Strong-Evidence	Luciferase reporter assay//RT-PCR//Western blot
Prediction-Evidence	MicroInspector
Support Database	ViRBase//VmiReg

References:

[1]PMID	22108199	Target region	3'UTR
Source	ViRBase//VmiReg	Interactor1 expression	None
Tissue or cell line	B95-8 cells//H1299 cells//HK1 cells//NCI-H1299 cells//NIH3T3 cells//SUNE1 cells	Interactor2 expression	Downregulation
Description	Here, we explored the possible role of the EBV encoded miR-BHRF-1-1 in regulating the host-virus interaction in NPC in late viral lytic stage by targeting p53. The data suggest that the absence of miR-BHRF1-1 in NPC cells is necessary to maintain EBV latent infection in NPC tumorigenesis.

[2]PMID	23170179	Target region	3'UTR
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	B95-8 cells//H1299 cells//HK1 cells//NCI-H1299 cells//NIH3T3 cells//SUNE1 cells	Interactor2 expression	Downregulation
Description	Each method has inherent advantages and limitations, but the combination of these approaches have been successful in identifying many miRNA targets (Table 1). Table 1 List of experimentally validated cellular targets of KSHV- and EBV- encoded miRNAs. The normal cellular functions of these proteins and the effect of their knockdown during viral infection are also listed. The targets that are underlined are repressed by both KSHV- and EBV-encoded miRNAs.