Basic Information:

  VIRBase ID:  

VHID00277357

  Virus:  

Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)

  Host:  

Homo sapiens

  Confidence Score:  

0.8847

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      ebv-miR-BHRF1-1:  MIMAT0000995          NASP:  NM_001195193     

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Interactor Information:

	Interactor1	Interactor2
Symbol	ebv-miR-BHRF1-1	NASP
miRBase Accession/Entrez ID	MIMAT0000995	4678
Organism	Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)	Homo sapiens
Category	miRNA	mRNA
Alias	-	FLB7527//HMDRA1//PRO1999

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	NASP	chr1:46075712(+) A-I      ncRNA chr1:46075634(+) A-I      ncRNA chr1:46075653(+) A-I      ncRNA chr1:46075826(+) A-I      ncRNA chr1:46076312(+) A-I      ncRNA chr1:46076351(+) A-I      ncRNA chr1:46076564(+) A-I      ncRNA chr1:46065200(+) A-I      intronic chr1:46075710(+) A-I      ncRNA chr1:46058461(+) A-I      intronic chr1:46058376(+) A-I      intronic chr1:46058332(+) A-I      intronic chr1:46058328(+) A-I      intronic chr1:46058075(+) A-I      intronic chr1:46058020(+) A-I      intronic chr1:46057254(+) A-I      intronic chr1:46057234(+) A-I      intronic chr1:46057209(+) A-I      intronic chr1:46057106(+) A-I      intronic chr1:46055005(+) A-I      intronic chr1:46054874(+) A-I      intronic chr1:46054867(+) A-I      intronic chr1:46054820(+) A-I      intronic chr1:46054577(+) A-I      intronic chr1:46054423(+) A-I      intronic chr1:46053962(+) A-I      intronic chr1:46053959(+) A-I      intronic chr1:46053933(+) A-I      intronic chr1:46060688(+) A-I      intronic chr1:46063982(+) A-I      intronic chr1:46063947(+) A-I      intronic chr1:46060765(+) A-I      intronic chr1:46062900(+) A-I      intronic chr1:46062858(+) A-I      intronic chr1:46062832(+) A-I      intronic chr1:46062829(+) A-I      intronic chr1:46062818(+) A-I      intronic chr1:46062795(+) A-I      intronic chr1:46062794(+) A-I      intronic chr1:46059054(+) A-I      intronic chr1:46059055(+) A-I      intronic chr1:46059082(+) A-I      intronic chr1:46059084(+) A-I      intronic chr1:46060331(+) A-I      intronic chr1:46059408(+) A-I      intronic chr1:46060318(+) A-I      intronic chr1:46060934(+) A-I      intronic chr1:46060969(+) A-I      intronic chr1:46062476(+) A-I      intronic chr1:46059921(+) A-I      intronic chr1:46060256(+) A-I      intronic chr1:46062504(+) A-I      intronic chr1:46062557(+) A-I      intronic chr1:46058287(+) A-I      intronic chr1:46053469(+) A-I      intronic chr1:46062967(+) A-I      intronic chr1:46076401(+) A-I      ncRNA chr1:46075777(+) A-I      ncRNA chr1:46075685(+) A-I      ncRNA chr1:46076573(+) A-I      ncRNA chr1:46075715(+) A-I      ncRNA chr1:46076524(+) A-I      ncRNA chr1:46075803(+) A-I      ncRNA chr1:46075623(+) A-I      ncRNA chr1:46076642(+) A-I      ncRNA chr1:46076481(+) A-I      ncRNA chr1:46076349(+) A-I      ncRNA chr1:46075612(+) A-I      ncRNA chr1:46075675(+) A-I      ncRNA chr1:46076468(+) A-I      ncRNA chr1:46076590(+) A-I      ncRNA chr1:46075719(+) A-I      ncRNA chr1:46075724(+) A-I      ncRNA chr1:46076529(+) A-I      ncRNA chr1:46075664(+) A-I      ncRNA chr1:46082719(+) A-I      intronic chr1:46059607(+) A-I      intronic chr1:46055071(+) A-I      intronic chr1:46062489(+) A-I      intronic chr1:46052147(+) A-I      intronic chr1:46060403(+) A-I      intronic chr1:46057103(+) A-I      intronic chr1:46053674(+) A-I      intronic chr1:46057246(+) A-I      intronic chr1:46062821(+) A-I      intronic chr1:46054350(+) A-I      intronic chr1:46055048(+) A-I      intronic chr1:46055026(+) A-I      intronic chr1:46065241(+) A-I      intronic chr1:46082119(+) A-I      intronic chr1:46064950(+) A-I      intronic chr1:46060768(+) A-I      intronic chr1:46059019(+) A-I      intronic chr1:46054433(+) A-I      intronic chr1:46054560(+) A-I      intronic chr1:46058426(+) A-I      intronic chr1:46055035(+) A-I      intronic chr1:46053698(+) A-I      intronic chr1:46062727(+) A-I      intronic chr1:46053807(+) A-I      intronic chr1:46057235(+) A-I      intronic chr1:46075601(+) A-I      ncRNA chr1:46076462(+) A-I      ncRNA chr1:46076543(+) A-I      ncRNA chr1:46076528(+) A-I      ncRNA chr1:46075604(+) A-I      ncRNA chr1:46076505(+) A-I      ncRNA chr1:46075783(+) A-I      ncRNA chr1:46076599(+) A-I      ncRNA chr1:46075694(+) A-I      ncRNA chr1:46075718(+) A-I      ncRNA chr1:46075760(+) A-I      ncRNA chr1:46075711(+) A-I      ncRNA chr1:46075709(+) A-I      ncRNA chr1:46075632(+) A-I      ncRNA chr1:46075780(+) A-I      ncRNA chr1:46075663(+) A-I      ncRNA chr1:46076358(+) A-I      ncRNA chr1:46075779(+) A-I      ncRNA chr1:46075720(+) A-I      ncRNA chr1:46076492(+) A-I      ncRNA chr1:46076406(+) A-I      ncRNA chr1:46076553(+) A-I      ncRNA chr1:46075767(+) A-I      ncRNA chr1:46076633(+) A-I      ncRNA chr1:46075672(+) A-I      ncRNA chr1:46075756(+) A-I      ncRNA chr1:46075610(+) A-I      ncRNA chr1:46075721(+) A-I      ncRNA chr1:46075648(+) A-I      ncRNA chr1:46076650(+) A-I      ncRNA chr1:46075695(+) A-I      ncRNA chr1:46075791(+) A-I      ncRNA chr1:46076488(+) A-I      ncRNA chr1:46076570(+) A-I      ncRNA chr1:46075618(+) A-I      ncRNA chr1:46076664(+) A-I      ncRNA chr1:46075684(+) A-I      ncRNA chr1:46075670(+) A-I      ncRNA chr1:46076500(+) A-I      ncRNA chr1:46075821(+) A-I      ncRNA chr1:46075713(+) A-I      ncRNA chr1:46076517(+) A-I      ncRNA chr1:46075661(+) A-I      ncRNA chr1:46075792(+) A-I      ncRNA chr1:46075805(+) A-I      ncRNA chr1:46076467(+) A-I      ncRNA chr1:46075626(+) A-I      ncRNA chr1:46075812(+) A-I      ncRNA chr1:46076533(+) A-I      ncRNA chr1:46056142(+) A-I      intronic chr1:46059998(+) A-I      intronic chr1:46059922(+) A-I      intronic chr1:46058326(+) A-I      intronic chr1:46054929(+) A-I      intronic chr1:46062748(+) A-I      intronic chr1:46059058(+) A-I      intronic chr1:46059603(+) A-I      intronic chr1:46062939(+) A-I      intronic chr1:46053578(+) A-I      intronic chr1:46054831(+) A-I      intronic chr1:46057129(+) A-I      intronic chr1:46057051(+) A-I      intronic chr1:46060718(+) A-I      intronic chr1:46060878(+) A-I      intronic chr1:46059040(+) A-I      intronic chr1:46062523(+) A-I      intronic chr1:46053973(+) A-I      intronic chr1:46059951(+) A-I      intronic chr1:46057076(+) A-I      intronic chr1:46065053(+) A-I      intronic chr1:46064931(+) A-I      intronic chr1:46065204(+) A-I      intronic chr1:46054183(+) A-I      intronic chr1:46057077(+) A-I      intronic chr1:46063936(+) A-I      intronic chr1:46068531(+) A-I      intronic chr1:46059415(+) A-I      intronic chr1:46082777(+) A-I      intronic chr1:46059630(+) A-I      intronic chr1:46060278(+) A-I      intronic chr1:46054824(+) A-I      intronic chr1:46054182(+) A-I      intronic chr1:46059003(+) A-I      intronic chr1:46079412(+) A-I      intronic chr1:46056141(+) A-I      intronic chr1:46062458(+) A-I      intronic chr1:46054853(+) A-I      intronic chr1:46057159(+) A-I      intronic chr1:46063894(+) A-I      intronic chr1:46082127(+) A-I      intronic chr1:46059881(+) A-I      intronic chr1:46053435(+) A-I      intronic chr1:46057840(+) A-I      intronic chr1:46058950(+) A-I      intronic chr1:46062615(+) A-I      intronic chr1:46054115(+) A-I      intronic chr1:46064993(+) A-I      intronic chr1:46054986(+) A-I      intronic chr1:46062924(+) A-I      intronic chr1:46064904(+) A-I      intronic chr1:46057198(+) A-I      intronic chr1:46065298(+) A-I      intronic chr1:46062771(+) A-I      intronic chr1:46059880(+) A-I      intronic chr1:46055386(+) A-I      intronic chr1:46054879(+) A-I      intronic chr1:46058408(+) A-I      intronic chr1:46058995(+) A-I      intronic chr1:46058973(+) A-I      intronic chr1:46057562(+) A-I      intronic chr1:46063943(+) A-I      intronic chr1:46062475(+) A-I      intronic chr1:46082201(+) A-I      intronic chr1:46079287(+) A-I      intronic chr1:46062455(+) A-I      intronic chr1:46062904(+) A-I      intronic chr1:46076903(+) A-I      intronic chr1:46062576(+) A-I      intronic chr1:46054848(+) A-I      intronic chr1:46062817(+) A-I      intronic chr1:46053657(+) A-I      intronic chr1:46057510(+) A-I      intronic chr1:46055019(+) A-I      intronic

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	NASP	chr1:46076462(+) A-I intron -    15342557   //15258596 chr1:46076467(+) A-I intron -    15342557   //15258596   //21960545 chr1:46076468(+) A-I intron -    15258596   //21960545 chr1:46076481(+) A-I intron -    15342557   //15258596   //21960545 chr1:46076529(+) A-I intron -    15342557   //15258596   //22327324 chr1:46076543(+) A-I intron -    15342557   //15258596 chr1:46076633(+) A-I intron -    15342557   //15258596 chr1:46076642(+) A-I intron -    15342557   //15258596   //22327324 chr1:46076903(+) A-I intron -    15342557 chr1:46078630(+) A-I intron -    15342557 chr1:46078854(+) A-I exon CDS    15342557 chr1:46075664(+) A-I intron -    21960545   //22327324 chr1:46075724(+) A-I intron -    22327324 chr1:46076533(+) A-I intron -    22327324 chr1:46075604(+) A-I intron -    21960545 chr1:46075612(+) A-I intron -    21960545 chr1:46075661(+) A-I intron -    21960545 chr1:46075663(+) A-I intron -    21960545 chr1:46053578(+) A-G intron -    22484847 chr1:46053698(+) A-G intron -    22484847 chr1:46053973(+) A-G intron -    22484847 chr1:46054115(+) A-G intron -    22484847 chr1:46054350(+) A-G intron -    22484847 chr1:46054433(+) A-G intron -    22484847 chr1:46054477(+) T-C intron -    22484847 chr1:46054824(+) A-G intron -    22484847 chr1:46054831(+) A-G intron -    22484847 chr1:46054848(+) A-G intron -    22484847 chr1:46054853(+) A-G intron -    22484847 chr1:46054879(+) A-G intron -    22484847 chr1:46054986(+) A-G intron -    22484847 chr1:46055026(+) A-G intron -    22484847 chr1:46055048(+) A-G intron -    22484847 chr1:46057051(+) A-G intron -    22484847 chr1:46057103(+) A-G intron -    22484847 chr1:46057129(+) A-G intron -    22484847 chr1:46057159(+) A-G intron -    22484847 chr1:46057198(+) A-G intron -    22484847 chr1:46057840(+) A-G intron -    22484847 chr1:46058326(+) A-G intron -    22484847 chr1:46058950(+) A-G intron -    22484847 chr1:46058973(+) A-G intron -    22484847 chr1:46058995(+) A-G intron -    22484847 chr1:46059003(+) A-G intron -    22484847 chr1:46059540(+) T-A intron -    22484847 chr1:46059951(+) A-G intron -    22484847 chr1:46062727(+) A-G intron -    22484847 chr1:46062748(+) A-G intron -    22484847 chr1:46062817(+) A-G intron -    22484847 chr1:46062821(+) A-G intron -    22484847 chr1:46062904(+) A-G intron -    22484847 chr1:46064796(+) T-G intron -    22484847 chr1:46064798(+) G-T intron -    22484847 chr1:46064993(+) A-G intron -    22484847 chr1:46065053(+) A-G intron -    22484847 chr1:46065204(+) A-G intron -    22484847 chr1:46071465(+) T-A intron -    22484847 chr1:46071636(+) T-C intron -    22484847 chr1:46075604(+) A-G intron -    22484847 chr1:46075612(+) A-G intron -    22484847 chr1:46075623(+) A-G intron -    22484847 chr1:46075632(+) A-G intron -    22484847 chr1:46075648(+) A-G intron -    22484847 chr1:46075661(+) A-G intron -    22484847 chr1:46075663(+) A-G intron -    22484847 chr1:46075664(+) A-G intron -    22484847 chr1:46075670(+) A-G intron -    22484847 chr1:46075715(+) A-G intron -    22484847 chr1:46075718(+) A-G intron -    22484847 chr1:46075719(+) A-G intron -    22484847 chr1:46075720(+) A-G intron -    22484847 chr1:46075724(+) A-G intron -    22484847 chr1:46075767(+) A-G intron -    22484847 chr1:46075777(+) A-G intron -    22484847 chr1:46075805(+) A-G intron -    22484847 chr1:46076406(+) A-G intron -    22484847 chr1:46076462(+) A-G intron -    22484847 chr1:46076467(+) A-G intron -    22484847 chr1:46076468(+) A-G intron -    22484847 chr1:46076481(+) A-G intron -    22484847 chr1:46076500(+) A-G intron -    22484847 chr1:46076505(+) A-G intron -    22484847 chr1:46076529(+) A-G intron -    22484847 chr1:46076533(+) A-G intron -    22484847 chr1:46076543(+) A-G intron -    22484847 chr1:46076599(+) A-G intron -    22484847 chr1:46076642(+) A-G intron -    22484847 chr1:46077218(+) A-G intron -    22484847 chr1:46077345(+) A-G intron -    22484847 chr1:46079412(+) A-G intron -    22484847 chr1:46082210(+) T-C intron -    22484847 chr1:46082211(+) T-C intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	NASP	chr1:46049536-46049537(+) m6A 5'UTR       24284625 chr1:46049830-46049831(+) Y 5'UTR//CDS//exon       25219674 chr1:46054584-46054585(+) m6A 3'UTR//intron       24209618 chr1:46054598-46054599(+) m6A 3'UTR//intron       24209618 chr1:46067953-46067954(+) m6A 3'UTR//CDS//exon//intron       24209618 chr1:46067968-46067969(+) m6A 3'UTR//CDS//exon//intron       24209618 chr1:46067974-46067975(+) m6A 3'UTR//CDS//exon//intron       24209618 chr1:46070626-46070627(+) m6A 3'UTR//CDS//exon//intron       24209618//24981863//27773535//22575960 chr1:46072209-46072210(+) m1A 3'UTR//CDS//exon//intron       26863196 chr1:46072220-46072221(+) m6A 3'UTR//CDS//exon//intron       24209618//24981863//27773535//22575960//22608085 chr1:46073017-46073018(+) m6A 3'UTR//CDS//exon//intron       24284625//24209618//25456834//24981863//27773535//22608085 chr1:46073049-46073050(+) m1A 3'UTR//CDS//exon//intron       26863196 chr1:46073060-46073061(+) m6A 3'UTR//CDS//exon//intron       24284625//24209618//25456834//24981863//27773535//22608085 chr1:46073067-46073068(+) m6A 3'UTR//CDS//exon//intron       24284625//24209618//25456834//24981863//27773535//22608085 chr1:46073090-46073091(+) m6A 3'UTR//CDS//exon//intron       24284625//24209618//25456834//24981863//27773535//22608085 chr1:46073101-46073102(+) m6A 3'UTR//CDS//exon//intron       24284625//24209618//25456834//24981863//27773535//22608085 chr1:46073106-46073107(+) m6A 3'UTR//CDS//exon//intron       24284625//24209618//25456834//24981863//27773535//22608085 chr1:46073207-46073208(+) m6A 3'UTR//CDS//exon//intron       24284625//24209618//25456834//24981863//27773535//22608085 chr1:46073216-46073217(+) Gm 3'UTR//CDS//exon//intron       - chr1:46073245-46073246(+) m6A 3'UTR//CDS//exon//intron       24284625//24209618//25456834//24981863//27773535//22608085 chr1:46073281-46073282(+) m6A 3'UTR//CDS//exon//intron       24284625//24209618//25456834//24981863//27773535//22608085 chr1:46073324-46073325(+) m6A 3'UTR//CDS//exon//intron       24284625//24209618//25456834//24981863//27773535//22608085 chr1:46073484-46073485(+) m6A 3'UTR//CDS//intron       24284625//24209618//25456834//24981863//27773535//22608085 chr1:46073575-46073576(+) m6A 3'UTR//CDS//intron       24209618//24981863//22575960 chr1:46073728-46073729(+) m6A 3'UTR//CDS//intron       24284625//24209618//25456834//24981863//27773535//22575960//22608085 chr1:46073750-46073751(+) m6A 3'UTR//CDS//intron       24284625//24209618//25456834//24981863//27773535//22575960//22608085 chr1:46073761-46073762(+) m6A 3'UTR//CDS//intron       24284625//24209618//25456834//24981863//27773535//22575960//22608085 chr1:46073768-46073769(+) m6A 3'UTR//CDS//intron       24284625//24209618//25456834//24981863//27773535//22575960//22608085 chr1:46073782-46073783(+) m6A 3'UTR//CDS//intron       24284625//24209618//25456834//24981863//27773535//22575960//22608085 chr1:46073792-46073793(+) m6A 3'UTR//CDS//intron       24284625//24209618//25456834//24981863//27773535//22575960//22608085 chr1:46073809-46073810(+) m6A 3'UTR//CDS//intron       24284625//24209618//25456834//24981863//27773535//22575960//22608085 chr1:46073855-46073856(+) m6A 3'UTR//CDS//intron       24284625//24209618//25456834//24981863//27773535//22575960//22608085 chr1:46073943-46073944(+) m6A 3'UTR//CDS//intron       24284625//24209618//24981863//27773535//22608085 chr1:46073972-46073973(+) m6A 3'UTR//CDS//intron       24209618//24981863//27773535//22608085 chr1:46073989-46073990(+) m6A 3'UTR//CDS//intron       24209618//24981863//27773535 chr1:46074985-46074986(+) m6A exon//intron       24209618//24981863//27773535 chr1:46075005-46075006(+) m6A exon//intron       24981863//27773535 chr1:46075019-46075020(+) m6A exon//intron       24981863//27773535 chr1:46075671-46075672(+) m6A exon//intron       - chr1:46075694-46075695(+) m6A exon//intron       - chr1:46078842-46078843(+) m6A 3'UTR//CDS//exon       24981863 chr1:46078847-46078848(+) Am 3'UTR//CDS//exon       - chr1:46078881-46078882(+) m6A 3'UTR//CDS//exon       24981863//27773535 chr1:46078915-46078916(+) m6A 3'UTR//CDS//exon       24981863//27773535 chr1:46079796-46079797(+) Y 3'UTR//CDS//exon       25219674 chr1:46079801-46079802(+) m6A 3'UTR//CDS//exon       27773535 chr1:46079971-46079972(+) m6A 3'UTR//CDS//exon       27773535 chr1:46080736-46080737(+) m6A 3'UTR//CDS//exon       22575960 chr1:46080773-46080774(+) m6A 3'UTR//CDS//exon       22575960 chr1:46080864-46080865(+) m6A 3'UTR//CDS//exon       24981863//27773535 chr1:46081043-46081044(+) m6A 3'UTR//CDS//exon       24981863//27773535 chr1:46081052-46081053(+) m6A 3'UTR//CDS//exon       24981863//27773535 chr1:46081121-46081122(+) m6A CDS//exon       24981863//27773535 chr1:46082029-46082030(+) Y CDS//exon       26075521 chr1:46082953-46082954(+) m6A exon//intron       24981863//27773535 chr1:46083010-46083011(+) m6A exon//intron       24981863//27773535 chr1:46083092-46083093(+) m6A exon//intron       24981863 chr1:46083139-46083140(+) m6A CDS//exon//intron       24981863//27773535 chr1:46083184-46083185(+) m6A CDS//exon//intron       24981863//27773535//22575960 chr1:46083249-46083250(+) m6A CDS//exon//intron       24981863//27773535//22575960 chr1:46083771-46083772(+) m6A CDS//exon       24981863//27773535//22575960 chr1:46084190-46084191(+) m6A 3'UTR       24284625 chr1:46084239-46084240(+) m6A 3'UTR       24284625//25456834//24981863//22608085 chr1:46084327-46084328(+) m6A 3'UTR       24284625//25456834//24981863//27773535//22608085 chr1:46084336-46084337(+) m6A 3'UTR       24284625//25456834//24981863//27773535//22608085 chr1:46084408-46084409(+) m6A 3'UTR       24284625//25456834//24981863//27773535//22608085 chr1:46084419-46084420(+) m6A 3'UTR       24284625//25456834//24981863//27773535//22608085

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	ebv-miR-BHRF1-1	Exosome Jurkat-derived T cell line (J77cl20)     21505438
	NASP	Chromatin K562 cells     - Cytosol Human myelogenous leukemia cell line (K-562)     21613539 Cytosol HeLa-S3 cells     - Exosome Blood     - Membrane K562 cells     - Nucleolus K562 cells     - Nucleoplasm K562 cells     - Nucleus Colon cancer cells     24393600 Nucleus HeLa-S3 cells|K562 cells     - Ribosome HEK-293 cells     22199352

Interaction Network (The top 100 interactions):

Interactor1: ebv-miR-BHRF1-1
Interactor2: NASP

Evidence Support:

Strong-Evidence	Luciferase reporter assay
Weak-Evidence	PAR-CLIP
Prediction-Evidence	PARalyzer
Support Database	ViRBase//VIRmiRNA

References:

PMID	22291592	Target region	CDS
Source	ViRBase//VIRmiRNA	Interactor1 expression	None
Tissue or cell line	EF3D-AGO2 cells//LCL35 cells//LCL-BAC cells//LCL-BAC-D1 cells//LCL-BAC-D3 cells	Interactor2 expression	Downregulation
Description	We identified an additional 2,972 CDS miRNA-interaction sites present in at least two libraries, 89.7% of which could be assigned to a miRNA (Table S7).