The nucleoplasmic location of the EBER1 RNP suggests that the formation of EBER1-L22 complexes in vivo could occur either prior to L22 assembly into the ribosome or by removal of L22 from preexisting ribosomes and subsequent sequestration in the nucleus.
EBER1 also forms an RNP with ribosomal protein L22; indeed, three molecules of ribosomal protein L22 can bind to EBER1 through three of its stem-loops (Fok et al. 2006a) (Fig.1B). The binding of L22 to EBER1 causes L22 to appear in the nucleoplasm, as well as its normal location in the nucleolus and cytoplasm (Toczyski et al. 1994).
