Basic Information:

  VIRBase ID:  

VHID00276922

  Virus:  

Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)

  Host:  

Homo sapiens

  Confidence Score:  

0.9526

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      Predicted binding sites in PRIdictor:   RPL22:  NP_000974     

Interactor Information:

	Interactor1	Interactor2
Symbol	EBER1	RPL22
miRBase Accession/Entrez ID	EBER1	6146
Organism	Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)	Homo sapiens
Category	lncRNA	protein
Alias	-	EAP//HBP15//HBP15/L22//L22

RNA Editing:

Resource	Symbol	Editing Position	Change	Genetic Region
RADAR	RPL22	chr1:6245417(-) A-I      3'UTR chr1:6245412(-) A-I      3'UTR chr1:6245411(-) A-I      3'UTR chr1:6245396(-) A-I      3'UTR chr1:6248220(-) A-I      intronic chr1:6248240(-) A-I      intronic chr1:6248248(-) A-I      intronic chr1:6248260(-) A-I      intronic chr1:6248286(-) A-I      intronic chr1:6248289(-) A-I      intronic chr1:6248330(-) A-I      intronic chr1:6250615(-) A-I      intronic chr1:6250647(-) A-I      intronic chr1:6249027(-) A-I      intronic chr1:6245395(-) A-I      3'UTR chr1:6252139(-) A-I      intronic chr1:6253558(-) A-I      intronic chr1:6253559(-) A-I      intronic chr1:6253562(-) A-I      intronic chr1:6253565(-) A-I      intronic chr1:6253576(-) A-I      intronic chr1:6245463(-) A-I      3'UTR chr1:6245462(-) A-I      3'UTR chr1:6245445(-) A-I      3'UTR chr1:6245436(-) A-I      3'UTR chr1:6245434(-) A-I      3'UTR chr1:6250643(-) A-I      intronic chr1:6250644(-) A-I      intronic chr1:6250652(-) A-I      intronic chr1:6251079(-) A-I      intronic chr1:6251813(-) A-I      intronic chr1:6251875(-) A-I      intronic chr1:6251996(-) A-I      intronic chr1:6249511(-) A-I      intronic chr1:6252194(-) A-I      intronic chr1:6249513(-) A-I      intronic chr1:6249823(-) A-I      intronic chr1:6249846(-) A-I      intronic chr1:6249869(-) A-I      intronic chr1:6249881(-) A-I      intronic chr1:6249894(-) A-I      intronic chr1:6255247(-) A-I      intronic chr1:6252589(-) A-I      intronic chr1:6257363(-) A-I      intronic chr1:6252596(-) A-I      intronic chr1:6255025(-) A-I      intronic chr1:6255031(-) A-I      intronic chr1:6252673(-) A-I      intronic chr1:6252697(-) A-I      intronic chr1:6252733(-) A-I      intronic chr1:6252768(-) A-I      intronic chr1:6253461(-) A-I      intronic chr1:6255153(-) A-I      intronic chr1:6255171(-) A-I      intronic chr1:6256011(-) A-I      intronic chr1:6255324(-) A-I      intronic chr1:6257291(-) A-I      intronic chr1:6255301(-) A-I      intronic chr1:6252642(-) A-I      intronic chr1:6253468(-) A-I      intronic chr1:6255742(-) A-I      intronic chr1:6257227(-) A-I      intronic chr1:6249024(-) A-I      intronic chr1:6249021(-) A-I      intronic chr1:6249018(-) A-I      intronic chr1:6249015(-) A-I      intronic chr1:6249010(-) A-I      intronic chr1:6248897(-) A-I      intronic chr1:6248558(-) A-I      intronic chr1:6250468(-) A-I      intronic chr1:6257381(-) A-I      intronic chr1:6255316(-) A-I      intronic chr1:6250467(-) A-I      intronic chr1:6254993(-) A-I      intronic chr1:6255014(-) A-I      intronic chr1:6257187(-) A-I      intronic chr1:6252532(-) A-I      intronic chr1:6252541(-) A-I      intronic chr1:6248306(-) A-I      intronic chr1:6248211(-) A-I      intronic chr1:6248203(-) A-I      intronic chr1:6248200(-) A-I      intronic chr1:6250506(-) A-I      intronic chr1:6250534(-) A-I      intronic chr1:6250562(-) A-I      intronic chr1:6250573(-) A-I      intronic chr1:6250579(-) A-I      intronic chr1:6250619(-) A-I      intronic chr1:6248506(-) A-I      intronic chr1:6248269(-) A-I      intronic chr1:6256135(-) A-I      intronic chr1:6252306(-) A-I      intronic chr1:6252575(-) A-I      intronic chr1:6249528(-) A-I      intronic chr1:6248578(-) A-I      intronic chr1:6249562(-) A-I      intronic chr1:6252186(-) A-I      intronic chr1:6255249(-) A-I      intronic chr1:6253340(-) A-I      intronic chr1:6249127(-) A-I      intronic chr1:6252540(-) A-I      intronic chr1:6250552(-) A-I      intronic chr1:6251983(-) A-I      intronic chr1:6249613(-) A-I      intronic chr1:6253633(-) A-I      intronic chr1:6249501(-) A-I      intronic chr1:6251118(-) A-I      intronic chr1:6257166(-) A-I      intronic chr1:6248491(-) A-I      intronic chr1:6252265(-) A-I      intronic chr1:6254094(-) A-I      intronic chr1:6248493(-) A-I      intronic chr1:6253656(-) A-I      intronic chr1:6252712(-) A-I      intronic chr1:6249536(-) A-I      intronic chr1:6249070(-) A-I      intronic chr1:6247526(-) A-I      intronic chr1:6248539(-) A-I      intronic chr1:6252676(-) A-I      intronic chr1:6253481(-) A-I      intronic chr1:6248983(-) A-I      intronic chr1:6252273(-) A-I      intronic chr1:6252167(-) A-I      intronic chr1:6248149(-) A-I      intronic chr1:6249763(-) A-I      intronic chr1:6251976(-) A-I      intronic chr1:6251067(-) A-I      intronic chr1:6248288(-) A-I      intronic chr1:6250613(-) A-I      intronic chr1:6249023(-) A-I      intronic chr1:6251977(-) A-I      intronic chr1:6251895(-) A-I      intronic chr1:6251853(-) A-I      intronic chr1:6252593(-) A-I      intronic chr1:6248532(-) A-I      intronic chr1:6248505(-) A-I      intronic chr1:6252647(-) A-I      intronic chr1:6252308(-) A-I      intronic chr1:6250633(-) A-I      intronic chr1:6252202(-) A-I      intronic chr1:6257362(-) A-I      intronic chr1:6248320(-) A-I      intronic chr1:6249979(-) A-I      intronic chr1:6249735(-) A-I      intronic chr1:6254907(-) A-I      intronic chr1:6248177(-) A-I      intronic chr1:6251121(-) A-I      intronic chr1:6255034(-) A-I      intronic chr1:6254074(-) A-I      intronic chr1:6254144(-) A-I      intronic chr1:6248364(-) A-I      intronic chr1:6251823(-) A-I      intronic chr1:6250537(-) A-I      intronic chr1:6254100(-) A-I      intronic chr1:6248229(-) A-I      intronic chr1:6252566(-) A-I      intronic chr1:6253522(-) A-I      intronic chr1:6251910(-) A-I      intronic chr1:6248371(-) A-I      intronic chr1:6257201(-) A-I      intronic chr1:6252720(-) A-I      intronic chr1:6252555(-) A-I      intronic chr1:6251866(-) A-I      intronic chr1:6252396(-) A-I      intronic chr1:6249617(-) A-I      intronic chr1:6248259(-) A-I      intronic chr1:6250504(-) A-I      intronic chr1:6252485(-) A-I      intronic chr1:6247308(-) A-I      intronic chr1:6250695(-) A-I      intronic chr1:6252325(-) A-I      intronic chr1:6253614(-) A-I      intronic chr1:6251163(-) A-I      intronic chr1:6252132(-) A-I      intronic chr1:6248334(-) A-I      intronic chr1:6250578(-) A-I      intronic chr1:6249118(-) A-I      intronic chr1:6250716(-) A-I      intronic chr1:6252238(-) A-I      intronic chr1:6254886(-) A-I      intronic chr1:6250666(-) A-I      intronic chr1:6253710(-) A-I      intronic chr1:6251296(-) A-I      intronic chr1:6251337(-) A-I      intronic chr1:6252590(-) A-I      intronic chr1:6252635(-) A-I      intronic chr1:6251248(-) A-I      intronic chr1:6248325(-) A-I      intronic chr1:6249078(-) A-I      intronic chr1:6247525(-) A-I      intronic chr1:6254836(-) A-I      intronic chr1:6248359(-) A-I      intronic chr1:6247485(-) A-I      intronic chr1:6250508(-) A-I      intronic chr1:6253729(-) A-I      intronic chr1:6253655(-) A-I      intronic chr1:6250637(-) A-I      intronic chr1:6251810(-) A-I      intronic chr1:6248405(-) A-I      intronic chr1:6253341(-) A-I      intronic chr1:6252162(-) A-I      intronic chr1:6252302(-) A-I      intronic chr1:6248942(-) A-I      intronic chr1:6252185(-) A-I      intronic chr1:6251255(-) A-I      intronic chr1:6252745(-) A-I      intronic chr1:6251901(-) A-I      intronic chr1:6250509(-) A-I      intronic chr1:6247507(-) A-I      intronic chr1:6255009(-) A-I      intronic chr1:6250657(-) A-I      intronic chr1:6256139(-) A-I      intronic chr1:6248256(-) A-I      intronic chr1:6254049(-) A-I      intronic chr1:6257349(-) A-I      intronic chr1:6252379(-) A-I      intronic chr1:6251238(-) A-I      intronic chr1:6251308(-) A-I      intronic chr1:6251246(-) A-I      intronic chr1:6253734(-) A-I      intronic chr1:6251892(-) A-I      intronic chr1:6251847(-) A-I      intronic chr1:6257295(-) A-I      intronic chr1:6249942(-) A-I      intronic chr1:6252224(-) A-I      intronic chr1:6253719(-) A-I      intronic chr1:6253586(-) A-I      intronic chr1:6249140(-) A-I      intronic chr1:6251252(-) A-I      intronic chr1:6251136(-) A-I      intronic chr1:6252637(-) A-I      intronic chr1:6254804(-) A-I      intronic chr1:6250610(-) A-I      intronic chr1:6252554(-) A-I      intronic chr1:6248957(-) A-I      intronic chr1:6248367(-) A-I      intronic chr1:6248984(-) A-I      intronic chr1:6249978(-) A-I      intronic chr1:6248365(-) A-I      intronic chr1:6250641(-) A-I      intronic chr1:6252247(-) A-I      intronic chr1:6251886(-) A-I      intronic chr1:6252501(-) A-I      intronic chr1:6248230(-) A-I      intronic chr1:6249119(-) A-I      intronic chr1:6248236(-) A-I      intronic chr1:6249943(-) A-I      intronic chr1:6249499(-) A-I      intronic chr1:6250501(-) A-I      intronic chr1:6252677(-) A-I      intronic chr1:6257228(-) A-I      intronic chr1:6248274(-) A-I      intronic chr1:6251911(-) A-I      intronic chr1:6252182(-) A-I      intronic chr1:6250605(-) A-I      intronic

Resource	Symbol	Editing Position	Change	SeqReg	exReg	PMID
DARNED	RPL22	chr1:6247197(-) T-C intron -    22484847 chr1:6247672(-) A-T intron -    22484847 chr1:6247673(-) G-T intron -    22484847 chr1:6247688(-) T-G intron -    22484847 chr1:6248229(-) A-G intron -    22484847 chr1:6248334(-) A-G intron -    22484847 chr1:6248364(-) A-G intron -    22484847 chr1:6248367(-) A-G intron -    22484847 chr1:6248371(-) A-G intron -    22484847 chr1:6249023(-) A-G intron -    22484847 chr1:6249078(-) A-G intron -    22484847 chr1:6250501(-) A-G intron -    22484847 chr1:6250504(-) A-G intron -    22484847 chr1:6250508(-) A-G intron -    22484847 chr1:6250509(-) A-G intron -    22484847 chr1:6250537(-) A-G intron -    22484847 chr1:6250578(-) A-G intron -    22484847 chr1:6250605(-) A-G intron -    22484847 chr1:6250641(-) A-G intron -    22484847 chr1:6250657(-) A-G intron -    22484847 chr1:6251118(-) A-G intron -    22484847 chr1:6251163(-) A-G intron -    22484847 chr1:6251246(-) A-G intron -    22484847 chr1:6251252(-) A-G intron -    22484847 chr1:6251255(-) A-G intron -    22484847 chr1:6251296(-) A-G intron -    22484847 chr1:6251810(-) A-G intron -    22484847 chr1:6251823(-) A-G intron -    22484847 chr1:6251886(-) A-G intron -    22484847 chr1:6251910(-) A-G intron -    22484847 chr1:6251911(-) A-G intron -    22484847 chr1:6251983(-) A-G intron -    22484847 chr1:6252185(-) A-G intron -    22484847 chr1:6252224(-) A-G intron -    22484847 chr1:6252247(-) A-G intron -    22484847 chr1:6252306(-) A-G intron -    22484847 chr1:6252325(-) A-G intron -    22484847 chr1:6252379(-) A-G intron -    22484847 chr1:6252396(-) A-G intron -    22484847 chr1:6252647(-) A-G intron -    22484847 chr1:6252676(-) A-G intron -    22484847 chr1:6252677(-) A-G intron -    22484847 chr1:6252720(-) A-G intron -    22484847 chr1:6252745(-) A-G intron -    22484847 chr1:6253710(-) A-G intron -    22484847 chr1:6254144(-) A-G intron -    22484847 chr1:6254886(-) A-G intron -    22484847 chr1:6254907(-) A-G intron -    22484847 chr1:6255009(-) A-G intron -    22484847 chr1:6257228(-) A-G intron -    22484847 chr1:6257295(-) A-G intron -    22484847 chr1:6257349(-) A-G intron -    22484847 chr1:6257362(-) A-G intron -    22484847

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	RPL22	chr1:6245937-6245938(-) m6A 3'UTR//intron       26404942 chr1:6246029-6246030(-) Y 3'UTR//intron       26075521 chr1:6246688-6246689(-) m6A 3'UTR//exon//intron       24981863//27773535 chr1:6246841-6246842(-) m1A CDS//exon//intron       26863196 chr1:6253067-6253068(-) m6A CDS//exon       24981863//27773535 chr1:6258264-6258265(-) m6A exon//intron       27773535 chr1:6258273-6258274(-) m6A exon//intron       27773535 chr1:6258285-6258286(-) m6A exon//intron       27773535 chr1:6258320-6258321(-) m6A exon//intron       27773535 chr1:6258369-6258370(-) m6A exon//intron       27773535 chr1:6259224-6259225(-) m6A exon//intron       27773536 chr1:6259269-6259270(-) m6A exon//intron       25456834//27773536 chr1:6259278-6259279(-) m1A exon//intron       26863196 chr1:6259321-6259322(-) m6A exon//intron       27773536 chr1:6259352-6259353(-) m6A exon//intron       27773536 chr1:6260558-6260559(-) m6A 5'UTR//intron       27371828 chr1:6260589-6260590(-) m6A 5'UTR//intron       27371828 chr1:6260645-6260646(-) m6A 5'UTR//intron       27371828 chr1:6260709-6260710(-) m6A 5'UTR//intron       27371828 chr1:6260752-6260753(-) m6A 5'UTR//intron       27371828 chr1:6260757-6260758(-) m6A 5'UTR//intron       27371828 chr1:6260833-6260834(-) m6A 5'UTR//intron       27371828

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	EBER1	EBV-postitive Burkitt lymphoma-derived cell line (Raji) Cytoplasm     1332043 EBV-postitive Burkitt lymphoma-derived cell line (Raji) Nucleus     1332043
	RPL22	Chromatin K562 cells     - Cytosol K562 cells     - Exosome Blood     - Nucleolus K562 cells     - Nucleoplasm K562 cells     - Ribosome Colon cancer cells     24393600

Interaction Network (The top 100 interactions):

Interactor1: EBER1
Interactor2: RPL22

Evidence Support:

Strong-Evidence	IP//ISH//Western blot
Support Database	ViRBase

References:

[1]PMID	8159770	Target region	None
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	BJAB cells//BJAB-B1 cells//Raji cells	Interactor2 expression	None
Description	The Epstein-Barr virus (EBV) small RNA EBER1 binds and relocalizes ribosomal protein L22 in EBV-infected human B lymphocytes. In situ hybridization indicates that the EBER RNPs are predominantly nucleoplasmic, suggesting that L22 relocalization correlates with binding to EBER1 in vivo.

[2]PMID	16556938	Target region	None
Source	ViRBase	Interactor1 expression	None
Tissue or cell line	B cells	Interactor2 expression	None
Description	Electrophoretic mobility-shift assays(EMSAs) show that recombinant L22(rL22) and maltose-binding protein(MBP)-tagged L22 protein bind EBER 1 in vitro, both forming three specific protein-dependent mobility shifts.