Detail Information

Basic Information:


  VIRBase ID:  

VHID00276920

  Virus:  

Human gammaherpesvirus 4 (Epstein-Barr virus, EPV)

  Host:  

Homo sapiens

  Confidence Score:  

0.9975

  Interaction Type:  

Virus-Host interaction

Interactor Information:


Interactor1 Interactor2
Symbol EBER1 DDX58
miRBase
Accession/Entrez ID		 EBER1 23586
Organism Human gammaherpesvirus 4 (Epstein-Barr virus, EPV) Homo sapiens
Category lncRNA mRNA
Alias - RIG-I//RIG1//RIGI//RLR-1//SGMRT2

RNA Editing:


Resource Symbol Editing Position Change Genetic Region
RADAR DDX58
chr9:32501381(-) A-I      intronic
chr9:32499647(-) A-I      intronic
chr9:32499649(-) A-I      intronic
chr9:32499657(-) A-I      intronic
chr9:32501386(-) A-I      intronic
chr9:32482774(-) A-I      intronic
chr9:32482775(-) A-I      intronic
chr9:32501496(-) A-I      intronic
chr9:32520987(-) A-I      intronic
chr9:32482749(-) A-I      intronic
chr9:32524707(-) A-I      intronic
chr9:32486278(-) A-I      intronic
chr9:32524761(-) A-I      intronic
chr9:32524777(-) A-I      intronic
chr9:32524783(-) A-I      intronic
chr9:32486405(-) A-I      intronic
chr9:32478338(-) A-I      intronic
chr9:32481585(-) A-I      intronic
chr9:32481708(-) A-I      intronic
chr9:32481712(-) A-I      intronic
chr9:32499591(-) A-I      intronic
chr9:32499592(-) A-I      intronic
chr9:32499614(-) A-I      intronic
chr9:32475078(-) A-I      intronic
chr9:32475088(-) A-I      intronic
chr9:32475114(-) A-I      intronic
chr9:32478322(-) A-I      intronic
chr9:32481730(-) A-I      intronic
chr9:32520964(-) A-I      intronic
chr9:32481748(-) A-I      intronic
chr9:32481778(-) A-I      intronic
chr9:32481855(-) A-I      intronic
chr9:32501292(-) A-I      intronic
chr9:32481737(-) A-I      intronic
chr9:32499623(-) A-I      intronic
chr9:32499629(-) A-I      intronic
chr9:32456407(-) A-I      3'UTR
chr9:32456374(-) A-I      3'UTR
chr9:32504018(-) A-I      intronic
chr9:32462601(-) A-I      intronic
chr9:32462576(-) A-I      intronic
chr9:32501394(-) A-I      intronic
chr9:32524686(-) A-I      intronic
chr9:32459156(-) A-I      intronic
chr9:32524833(-) A-I      intronic
chr9:32524708(-) A-I      intronic
chr9:32495796(-) A-I      intronic
chr9:32482794(-) A-I      intronic
chr9:32498818(-) A-I      intronic
chr9:32462985(-) A-I      intronic
chr9:32501291(-) A-I      intronic
chr9:32456507(-) A-I      3'UTR
chr9:32456480(-) A-I      3'UTR
chr9:32456367(-) A-I      3'UTR
chr9:32481829(-) A-I      intronic
chr9:32524679(-) A-I      intronic
chr9:32524755(-) A-I      intronic
chr9:32462963(-) A-I      intronic
chr9:32482677(-) A-I      intronic
chr9:32481777(-) A-I      intronic
chr9:32524706(-) A-I      intronic
chr9:32481878(-) A-I      intronic
chr9:32463039(-) A-I      intronic
chr9:32501275(-) A-I      intronic
chr9:32482748(-) A-I      intronic
chr9:32473422(-) A-I      intronic
chr9:32481681(-) A-I      intronic
chr9:32462625(-) A-I      intronic
chr9:32463047(-) A-I      intronic
chr9:32481754(-) A-I      intronic
chr9:32524716(-) A-I      intronic
Resource Symbol Editing Position Change SeqReg exReg PMID
DARNED DDX58
chr9:32455466(-) A-I exon 3'UTR    15258596
chr9:32456316(-) A-I exon 3'UTR    15258596
chr9:32456318(-) A-I exon 3'UTR    15258596   //21960545
chr9:32456365(-) A-I exon 3'UTR    15258596   //19478186
chr9:32456380(-) A-I exon 3'UTR    15258596
chr9:32456486(-) A-I exon 3'UTR    15258596   //22327324
chr9:32456488(-) A-I exon 3'UTR    15258596
chr9:32456316(-) A-G exon 3'UTR    21725310   //22484847
chr9:32456486(-) A-G exon 3'UTR    21725310
chr9:32479714(-) T-C intron -    22484847
chr9:32456302(-) A-G exon 3'UTR    22484847
chr9:32456318(-) A-G exon 3'UTR    22484847
chr9:32456365(-) A-G exon 3'UTR    22484847
chr9:32456368(-) A-G exon 3'UTR    22484847
chr9:32456371(-) A-G exon 3'UTR    22484847
chr9:32456380(-) A-G exon 3'UTR    22484847
chr9:32456413(-) A-G exon 3'UTR    22484847
chr9:32456449(-) A-G exon 3'UTR    22484847
chr9:32456453(-) A-G exon 3'UTR    22484847

RNA Modification:


Resource Symbol ModificationPosition Type Genomic Context PMID
RMBase DDX58
chr9:32457063-32457064(-) m6A 3'UTR       24209618//24981863//27773535//27371828//22575960//22608085
chr9:32457168-32457169(-) m6A CDS       24284625//24209618//25456834//24981863//27773535//27371828//22575960//22608085
chr9:32457190-32457191(-) m6A CDS       24284625//24209618//25456834//24981863//27773535//27371828//22575960//22608085
chr9:32457256-32457257(-) m6A CDS       24284625//24981863//27773535//27371828//22608085
chr9:32457294-32457295(-) m6A CDS       24284625//24981863//27773535//27371828//22608085
chr9:32457362-32457363(-) m6A CDS       24981863//27773535
chr9:32467765-32467766(-) m6A CDS       27773535
chr9:32476997-32476998(-) m6A CDS       27773535
chr9:32477021-32477022(-) m6A CDS       27773535
chr9:32477029-32477030(-) m6A CDS       27773535
chr9:32477065-32477066(-) m6A CDS       27773535
chr9:32477073-32477074(-) m6A CDS       27773535
chr9:32526102-32526103(-) m6A 5'UTR//CDS       24981863
chr9:32526109-32526110(-) m6A 5'UTR//CDS       24981863

RNA Localization:


Resource Symbol Subcellular Localization Tissue or Cell Line PMID
RNALocate EBER1
EBV-postitive Burkitt lymphoma-derived cell line (Raji) Cytoplasm     1332043
EBV-postitive Burkitt lymphoma-derived cell line (Raji) Nucleus     1332043
DDX58
Cytosol HCC cell line (HepG2)|K562 cells     -
Endoplasmic reticulum Human myelogenous leukemia cell line (K-562)     21613539
Exosome Blood     -
Insoluble cytoplasm HCC cell line (HepG2)     -
Nucleus HCC cell line (HepG2)|HeLa-S3 cells     -
Ribosome HEK-293 cells     22199352

Interaction Network (The top 100 interactions):


Interactor1: EBER1
Interactor2: DDX58

Evidence Support:


Strong-Evidence Dual luciferase reporter assay//ELISA//IP//Luciferase reporter assay//RT-PCR//Western blot
Support Database ViRBase

References:


[1]PMID 16946700 Target region None
Source ViRBase Interactor1 expression None
Tissue or cell line Daudi cells//Mutu cells Interactor2 expression Downregulation
Description The induction of IFN-beta peaked as early as 6 h after transfection, suggesting that EBERs readily interacted with RIG-I after being expressed from the transgene (Figure 3B).
[2]PMID 18362887 Target region None
Source ViRBase Interactor1 expression None
Tissue or cell line Akata cells//BL cells//Mutu cells Interactor2 expression Downregulation
Description Furthermore, it was demonstrated that only EBER1 as well as only EBER2 could induce IL-10 in RIG-I expressing cell clones (Figure 2f).
[3]PMID 20399962 Target region None
Source ViRBase Interactor1 expression None
Tissue or cell line Akata cells//BL cells//Mutu cells Interactor2 expression Downregulation
Description Previous studies demonstrated that EBERs (EBER1 and EBER2) play significant roles in various EBV-infected cancer cells. EBERs induce the expression of interleukin(IL)-10 in BL cells, insulin-like growth factor(IGF)-1 in gastric carcinoma and nasopharyngeal carcinoma cells, IL-9 in T cells that act as an autocrine growth factor. It was also reported that EBERs play critical roles in the B cell growth transformation including IL-6 induction by EBER2.Recently, it was demonstrated that EBERs bind RIG-I and activate its downstream signaling, which induces expression of type-I interferon (IFN)s.

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