Basic Information:

  VIRBase ID:  

VHID00263485

  Virus:  

Human betaherpesvirus 5 (HHV-5)

  Host:  

Homo sapiens

  Confidence Score:  

0.9526

  Interaction Type:  

Virus-Host interaction

  Predicted Binding:

 

      hcmv-miR-US22-3p:  MIMAT0028166          EGR1:  NM_001964     

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Interactor Information:

	Interactor1	Interactor2
Symbol	hcmv-miR-US22-3p	EGR1
miRBase Accession/Entrez ID	MIMAT0028166	1958
Organism	Human betaherpesvirus 5 (HHV-5)	Homo sapiens
Category	miRNA	mRNA
Alias	-	AT225//G0S30//KROX-24//NGFI-A//TIS8//ZIF-268//ZNF225

RNA Modification:

Resource	Symbol	ModificationPosition	Type	Genomic Context	PMID
RMBase	EGR1	chr5:137801187-137801188(+) m6A 5'UTR       24981863 chr5:137801242-137801243(+) m6A 5'UTR       24209618//24981863 chr5:137801538-137801539(+) m6A CDS       26404942 chr5:137801721-137801722(+) m6A CDS       24209618//24981863//26404942 chr5:137802469-137802470(+) m6A CDS       24209618//24981863//26404942 chr5:137802492-137802493(+) m6A CDS       24209618//24981863//26404942 chr5:137802510-137802511(+) m6A CDS       24209618//24981863//26404942 chr5:137802775-137802776(+) m6A CDS       24284625//24209618//25456834//24981863//26404942//22575960 chr5:137802828-137802829(+) m6A CDS       24284625//24209618//25456834//24981863//26404942//22575960 chr5:137802946-137802947(+) m6A CDS       24284625//24209618//24981863//26404942 chr5:137803045-137803046(+) m6A CDS       24284625//24209618//24981863//26404942 chr5:137803088-137803089(+) m6A CDS       24284625//24209618//24981863//26404942 chr5:137803405-137803406(+) m6A CDS       24284625//24209618//25456834//24981863//26404942 chr5:137803417-137803418(+) m6A CDS       24284625//24209618//25456834//24981863//26404942 chr5:137803726-137803727(+) m6A CDS       24284625//24209618//25456834//24981863//26404942//22575960//22608085 chr5:137803752-137803753(+) m6A CDS       24284625//24209618//25456834//24981863//26404942//22575960//22608085 chr5:137803808-137803809(+) m6A 3'UTR       24209618//25456834//24981863//26404942//22575960//22608085 chr5:137803817-137803818(+) m6A 3'UTR       24209618//25456834//24981863//26404942//22575960//22608085 chr5:137803826-137803827(+) m6A 3'UTR       24209618//25456834//24981863//26404942//22575960//22608085 chr5:137803995-137803996(+) m6A 3'UTR       24209618//24981863//26404942//22575960 chr5:137804031-137804032(+) m6A 3'UTR       24981863//26404942//22575960 chr5:137804535-137804536(+) Y 3'UTR       25219674 chr5:137804592-137804593(+) m6A 3'UTR       24981863 chr5:137804705-137804706(+) m6A 3'UTR       24981863 chr5:137804717-137804718(+) m6A 3'UTR       24981863 chr5:137804728-137804729(+) m6A 3'UTR       24981863

RNA Localization:

Resource	Symbol	Subcellular Localization	Tissue or Cell Line	PMID
RNALocate	EGR1	Chromatin K562 cells     - Cytoplasm Nasopharyngeal carcinoma cells     20498841 Cytosol Human myelogenous leukemia cell line (K-562)     21613539 Exosome Blood     - Nucleoplasm K562 cells     - Nucleus Nasopharyngeal carcinoma cells     20498841 Nucleus HeLa-S3 cells     -

Interaction Network (The top 100 interactions):

Interactor1: hcmv-miR-US22-3p
Interactor2: EGR1

Evidence Support:

Strong-Evidence	Luciferase reporter assay//qRT-PCR//Western blot
Support Database	ViRBase

References:

PMID	31725809	Target region	3'UTR
Source	ViRBase	Interactor1 expression	Upregulation
Tissue or cell line	HEK 293 cells//NHDF cells	Interactor2 expression	Downregulation
Description	A 3-4 fold decrease in luciferase reporter activity was observed with transfection of miR-US22. In contrast, transfection of miR-US5-1 and miR-UL112-3p resulted in up to a two-fold increase in promoter activity.